Processing of cellulose synthase (AcsAB) from Gluconacetobacter hansenii 23769.

abstract：:The state of the various, spectroscopically distinguishable copper sites (type 1, type 2, and type 3 copper) of human ceruloplasmin was investigated by electron spin resonance (ESR) spectroscopy. The ESR measurements were performed at 100 K and at X-band during the reaction of the protein with either ascorbate or with...

journal_title：Archives of biochemistry and biophysics

authors： Musci G,Bonaccorsi di Patti MC,Calabrese L

更新日期：1993-10-01 00:00:00

abstract：:Two cDNAs encoding taxoid-O-acetyl transferases (TAX 9 and TAX 14) were obtained from a previously isolated family of Taxus acyl/aroyl transferase cDNA clones. The recombinant enzymes catalyze the acetylation of taxadien-5alpha,13alpha-diacetoxy-9alpha,10beta-diol to generate taxadien-5alpha,10beta,13alpha-tri-acetoxy...

journal_title：Archives of biochemistry and biophysics

authors： Hampel D,Mau CJ,Croteau RB

更新日期：2009-07-15 00:00:00

abstract：:Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool to investigate the structure and dynamics of RNA, because many biologically important RNAs have conformationally flexible structures, which makes them difficult to crystallize. Functional, independently folded RNA domains, range in size between simple st...

journal_title：Archives of biochemistry and biophysics

authors： Barnwal RP,Yang F,Varani G

更新日期：2017-08-15 00:00:00

abstract：:The influences of selenium deficiency (Se-D), chronic training, and an acute bout of exercise on hepatic and skeletal muscle antioxidant enzymes, i.e., superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX), as well as glutathione S-transferase (GST) and tissue lipid peroxidation, were investigated in ...

journal_title：Archives of biochemistry and biophysics

authors： Ji LL,Stratman FW,Lardy HA

更新日期：1988-05-15 00:00:00

abstract：:Oxygenation of a tryptophan residue analog by ascorbate in the presence of catalytic amounts of iron(II) and ethylenediaminetetraacetic acid (EDTA) has been studied. Under physiological conditions, reaction of the tryptophan derivative (N-t-butoxycarbonyl-L-tryptophan) with Fe(II)-EDTA and ascorbate resulted mainly in...

journal_title：Archives of biochemistry and biophysics

authors： Uchida K,Enomoto N,Itakura K,Kawakishi S

更新日期：1990-05-15 00:00:00

abstract：:We have examined the intrinsic fluorescence properties of a highly purified chloroplast H(+)-ATPase (CF0F1) preparation [R. D. Kirch and P. Graber (1992) Acta Physiol. Scand. 746, 9-12). Unlike the catalytic CF1 portion alone, CF0F1 fluorescence was dominated by tryptophan fluorescence both at 277-nm excitation, favor...

journal_title：Archives of biochemistry and biophysics

authors： Kirch RD,Colvert KK,Richter ML,Gräber P

更新日期：1995-02-20 00:00:00

abstract：:The velocity of acetaldehyde metabolism in rat liver may be governed either by the rate of regeneration of NAD from NADH through the electron transport system or by the activity of aldehyde dehydrogenase (ALDH). Measurements of oxygen consumption revealed that the electron transport system was capable of reoxidizing A...

journal_title：Archives of biochemistry and biophysics

authors： Svanas GW,Weiner H

更新日期：1985-01-01 00:00:00

abstract：:The pH gradient, delta pH, present across the inner mitochondrial membrane in isolated rat hepatocytes was continuously monitored with a novel spectroscopic technique that utilizes the weak acid fluorescein. Unlike most cytosolic pH indicators, such as 2',7'-bis(carboxyethyl)-5,(6)-carboxyfluorescein (BCECF), fluoresc...

journal_title：Archives of biochemistry and biophysics

authors： Thomas PJ,Gaspers LD,Pharr C,Thomas JA

更新日期：1991-07-01 00:00:00

abstract：:Vitamin E (vE) is a biological free radical scavenger capable of providing antioxidant protection depending upon its tissue content. In previous studies, we observed that vE increased significantly in rat lungs after oxidant exposure, and we postulated that vE may be mobilized to the lung from other body sites under o...

journal_title：Archives of biochemistry and biophysics

authors： Elsayed NM,Mustafa MG,Mead JF

更新日期：1990-11-01 00:00:00

abstract：:In contrast to other P450 enzymes purified from rat liver microsomes, purified P450 IIIA1 (P450p) is catalytically inactive when reconstituted with NADPH-cytochrome P450 reductase and the synthetic lipid, dilauroylphosphatidylcholine. However, purified P450 IIIA1 catalyzes the oxidation of testosterone when reconstitu...

journal_title：Archives of biochemistry and biophysics

authors： Eberhart DC,Parkinson A

更新日期：1991-12-01 00:00:00

abstract：:Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and b...

journal_title：Archives of biochemistry and biophysics

authors： Kakizaki I,Itano N,Kimata K,Hanada K,Kon A,Yamaguchi M,Takahashi T,Takagaki K

更新日期：2008-03-01 00:00:00

abstract：:Although an elastolytic activity in eosinophil-rich cell fractions from mice has been reported, this enzyme has not been purified and characterized as yet in any mammalian species. Eosinophilic elastase was isolated from human eosinophil fragments (cytosomes) obtained from normal and eosinophilic subjects. The enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： Lungarella G,Menegazzi R,Gardi C,Spessotto P,de Santi MM,Bertoncin P,Patriarca P,Calzoni P,Zabucchi G

更新日期：1992-01-01 00:00:00

abstract：:Rat hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was purified to homogeneity using agarose-HMG-CoA affinity chromatography. Additional protein was isolated from the affinity column with 0.5 M KCl that demonstrated no HMG-CoA reductase activity, yet comigrated with purified HMG-CoA reductase on sod...

journal_title：Archives of biochemistry and biophysics

authors： Whitehead TR,Vlahcevic ZR,Beg ZH,Hylemon PB

更新日期：1984-05-01 00:00:00

abstract：:The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca2+ transients (CaT) of mouse single cardiomyocytes were simultaneously ...

journal_title：Archives of biochemistry and biophysics

authors： Hayamizu K,Morimoto S,Nonaka M,Hoka S,Sasaguri T

更新日期：2018-01-01 00:00:00

abstract：:Iron-sulfur [Fe-S] clusters are ubiquitous ancient prosthetic groups that are required to sustain fundamental life processes. Formation of intracellular [Fe-S] clusters does not occur spontaneously but requires a complex biosynthetic machinery. Different types of [Fe-S] cluster assembly systems have been discovered. A...

journal_title：Archives of biochemistry and biophysics

authors： Fontecave M,Ollagnier-de-Choudens S

更新日期：2008-06-15 00:00:00

abstract：:The paper reports a study on the reactions of grass pea (Lathyrus sativus) amine oxidase (GPAO) with several aliphatic diamines. The influence of the chain length and of unsaturations in the molecules was examined. Kinetic measurements confirmed that trans-, i.e., (E)-2-butene-1,4-diamine (TDABE) and cis-, i.e., (Z)-2...

journal_title：Archives of biochemistry and biophysics

authors： Sebela M,Frébort I,Lemr K,Brauner F,Pec P

更新日期：2000-12-01 00:00:00

abstract：:Antioxidative flavonoids, ubiquitously included in vegetables, fruits and teas, are expected to prevent degenerative diseases. It is unclear, however, whether flavonoids can enter the cellular nuclei and suppress the oxidative damage of DNA. Here, several flavonoids at the physiological concentration of 10 microM were...

journal_title：Archives of biochemistry and biophysics

authors： Kanazawa K,Uehara M,Yanagitani H,Hashimoto T

更新日期：2006-11-15 00:00:00

abstract：:Bacillus subtilis 168 EpsC is annotated as "Probable polysaccharide biosynthesis protein" in the SwissProt database. epsC is part of the eps operon, thought to be involved in the biosynthesis of exopolymeric substances (EPS). The present study was undertaken to determine the molecular function of EpsC. Sequence analys...

journal_title：Archives of biochemistry and biophysics

authors： Kaundinya CR,Savithri HS,Krishnamurthy Rao K,Balaji PV

更新日期：2018-11-01 00:00:00

abstract：:Three forms of 20-kDa myosin light chain (MLC), unphosphorylated, monophosphorylated, and diphosphorylated MLC (designated 20K, 20K-P, and 20K-PP) were demonstrated in thrombin-stimulated human platelets by two different gel electrophoretic methods: in the presence of glycerol urea or in two dimensions (isoelectric an...

journal_title：Archives of biochemistry and biophysics

authors： Naka M,Saitoh M,Hidaka H

更新日期：1988-03-01 00:00:00

abstract：:The mitochondrial glycerol phosphate dehydrogenase (mGPD) is one of several proteins that are abundant in the pancreatic islet. Hormonal and nutritional influences confer tissue-specific patterns of expression on many of these proteins and the primary amino acid sequence of these proteins in the islet often differs fr...

journal_title：Archives of biochemistry and biophysics

authors： MacDonald MJ,Moran SM,Simonson GD

更新日期：1995-05-10 00:00:00

abstract：:The specific activity of cytochrome P450-linked coumarin 7-hydroxylase (COH) of hepatic mitoplasts from DBA/2N mice is up to 55% as great as the microsomal activity. According to Western blot and immunodiffusion analysis and inhibition studies with anti-P450Coh and metyrapone, the mitoplastic P450Coh had the same mole...

journal_title：Archives of biochemistry and biophysics

authors： Honkakoski P,Kojo A,Raunio H,Pasanen M,Juvonen R,Lang MA

更新日期：1988-12-01 00:00:00

abstract：:Numerous conditions induce expression of manganese-containing superoxide dismutase (MnSOD) in mammalian cells. The reported inducers of MnSOD are all agents that activate two transcription factors, AP-1 and NF kappa B, but several reports have suggested that MnSOD induction relies solely on NF kappa B. We investigated...

journal_title：Archives of biochemistry and biophysics

authors： Borrello S,Demple B

更新日期：1997-12-15 00:00:00

abstract：:(+)-Menthofuran is an undesirable monoterpenoid component of peppermint (Mentha x piperita) essential oil that is derived from the alpha,beta-unsaturated ketone (+)-pulegone. Microsomal preparations, from the oil gland secretory cells of a high (+)-menthofuran-producing chemotype of Mentha pulegium, transform (+)-pule...

journal_title：Archives of biochemistry and biophysics

authors： Bertea CM,Schalk M,Karp F,Maffei M,Croteau R

更新日期：2001-06-15 00:00:00

abstract：:Rats fed an ethanol-containing diet for 4 weeks showed a 3- to 5-fold increase over isocalorically pair-fed controls with respect to cytosolic NAD(P)H-quinone oxidoreductase (NQOR) (E.C.1.6.99.2) with both menadione and dichlorophenol-indophenol as substrates. Rates of NAD(P)H-dependent p-nitrosophenol (pNSP) reductio...

journal_title：Archives of biochemistry and biophysics

authors： Hajos AK,Winston GW

更新日期：1992-06-01 00:00:00

abstract：:Previous studies have suggested that pyroptosis may play an important role in LPS-induced acute lung injury (ALI), but the exact mechanism of pyroptosis induction and the role of Angiotensin-converting enzyme 2 (ACE2)/Ang (1-7)/Mas axis in pyroptosis has not been investigated yet. The present study aimed to establish ...

journal_title：Archives of biochemistry and biophysics

authors： Huang H,Wang J,Liu Z,Gao F

更新日期：2020-10-30 00:00:00

abstract：:Carbamoyl phosphate synthetase I (CPSase I) catalyzes the first reaction of the urea cycle in mammalian ureotelic species. The positive allosteric cofactor N-acetyl-L-glutamate (AGA) is required for CPSase I activity and is important for regulation of the urea cycle. A similar enzyme, CPSase III, catalyzes this reacti...

journal_title：Archives of biochemistry and biophysics

authors： Julsrud EA,Walsh PJ,Anderson PM

更新日期：1998-02-01 00:00:00

abstract：:The steric course of methyl group transfer catalyzed by two DNA methylases, HhaI methylase, a DNA (cytosine-5)-methyltransferase, and EcoRI methylase, which methylates at N6 of adenosine, has been studied with (methyl-R)- and (methyl-S)-[methyl-2H1,3H]adenosylmethionine as the methyl donor, using as substrates poly-d(...

journal_title：Archives of biochemistry and biophysics

authors： Ho DK,Wu JC,Santi DV,Floss HG

更新日期：1991-02-01 00:00:00

abstract：:Interleukin-1 alpha (IL-1) stimulated the release of degraded proteoglycan from primary cultures of chondrocyte monolayers in a time- and dose-dependent fashion. Bafilomycin A1, a specific inhibitor of the vacuolar H(+)-ATPase, efficiently blocked acidification of the chondrocyte vacuolar system. Under these condition...

journal_title：Archives of biochemistry and biophysics

authors： Yocum SA,Lopresti-Morrow LL,Gabel CA,Milici AJ,Mitchell PG

更新日期：1995-02-01 00:00:00

abstract：:The reduction of duroquinone (DQ), 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone (DB), and dichlorophenol indophenol (DCIP) by succinate and NADH was investigated in yeast mitochondria which have no spectrally detectable cytochrome b. Succinate reduces DB in the cytochrome b-deficient mitochondria at rates comparabl...

journal_title：Archives of biochemistry and biophysics

authors： Zhu QS,Sprague SG,Beattie DS

更新日期：1988-09-01 00:00:00

abstract：:In the present study we show that monospecific antibody against cytochrome P-450a completely inhibits testosterone 7 alpha-hydroxylation in hepatic microsomes of untreated male or female rats or rats of either sex treated with dexamethasone. These data are in contrast with those of K. Nagata et al. (1987, J. Biol. Che...

journal_title：Archives of biochemistry and biophysics

authors： Levin W,Thomas PE,Ryan DE,Wood AW

更新日期：1987-11-01 00:00:00