Stereochemical studies of the C-methylation of deoxycytidine catalyzed by HhaI methylase and the N-methylation of deoxyadenosine catalyzed by EcoRI methylase.

abstract：:Bovine collateral ligament synthesized a 35S-labeled large proteoglycan species which eluted with a Kav of approximately 0.27 on Sepharose CL-2B and contained only chondroitin sulfate chains with a molecular mass of approximately 32 kDa. Fluorography of the 35S-labeled core proteins derived from the large ligament pro...

journal_title：Archives of biochemistry and biophysics

authors： Campbell MA,Tester AM,Handley CJ,Checkley GJ,Chow GL,Cant AE,Winter AD,Cain WE

更新日期：1996-05-15 00:00:00

abstract：:Aldehyde oxidase (EC 1.2.3.1) plays a role in the oxidation of aromatic heterocyclic compounds ingested by some higher vertebrates. To better understand this function, the specificity of the rabbit liver enzyme toward purines and their analogs was quantitatively studied. The chemical nature of the 6-substituent of pur...

journal_title：Archives of biochemistry and biophysics

authors： Hall WW,Krenitsky TA

更新日期：1986-11-15 00:00:00

abstract：:The interactions of Cu, Zn superoxide dismutase (SOD) with nitroxyl (NO-) and nitric oxide (NO), both of which are thought to be biologically significant, have been studied but remain undefined. Having previously noted that NO- can reduce Cu (II), Zn SOD aerobically, we now report that it also can do so anaerobically ...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：2002-06-15 00:00:00

abstract：:It has now been more than 20years since the vitamin D receptor was identified in cells of the immune system. The immune system has now been established as an important target of vitamin D. Vitamin D receptor knockout and vitamin D deficient mice have a surplus of effector T cells that have been implicated in the patho...

journal_title：Archives of biochemistry and biophysics

authors： Cantorna MT

更新日期：2012-07-01 00:00:00

abstract：:Much has been written recently on osteoclast biology, but this cell type still astonishes scientists with its multifaceted functions and unique properties. The last three decades have seen a change in thinking about the osteoclast, from a cell with a single function, which just destroys the tissue it belongs to, to an...

journal_title：Archives of biochemistry and biophysics

authors： Cappariello A,Maurizi A,Veeriah V,Teti A

更新日期：2014-11-01 00:00:00

abstract：:The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

journal_title：Archives of biochemistry and biophysics

authors： Salvador JM,Mata AM

更新日期：1998-03-15 00:00:00

abstract：:A catalase-related allene oxide synthase (cAOS) and true catalases that metabolize hydrogen peroxide have similar structure around the heme. One of the distal heme residues considered to help control catalysis is a highly conserved asparagine. Here we addressed the role of this residue in metabolism of the natural sub...

journal_title：Archives of biochemistry and biophysics

authors： Gao B,Boeglin WE,Brash AR

更新日期：2008-09-15 00:00:00

abstract：:One of the key steps involved in T-cell activation is binding of the tyrosine kinase ZAP-70 via its two SH2 domains to peptide segments termed tyrosine-based activation motifs (ITAM) which are present in three of the T-cell receptor (TCR) subunits. The crystal structure of the ZAP-70 SH2 domains complexed to phosphope...

journal_title：Archives of biochemistry and biophysics

authors： Labadia ME,Jakes S,Grygon CA,Greenwood DJ,Schembri-King J,Lukas SM,Warren TC,Ingraham RH

更新日期：1997-06-01 00:00:00

abstract：:Fresh human A1 erythrocytes, washed and pretreated in phosphate buffer with or without papain, were incubated at 37 degrees C with blood group-degrading enzymes from the human fecal Ruminococcus torques strain IX-70. The effects were assayed as changes in hemagglutination patterns, and blood group activities of alkali...

journal_title：Archives of biochemistry and biophysics

authors： Falk P,Hoskins LC,Lindstedt R,Svanborg C,Larson G

更新日期：1991-11-01 00:00:00

abstract：:Two new types of bacterial Fe-S L-serine dehydratases have been identified. These join two previously recognized enzyme types, for a total of four, that are distinguished on the basis of domain arrangement and amino acid sequence. A Type 3 enzyme from Amphibacillus xylanus (axLSD) and a Type 4 enzyme from Heliscomenob...

journal_title：Archives of biochemistry and biophysics

authors： Xu XL,Grant GA

更新日期：2013-12-01 00:00:00

abstract：:Xenopus laevis nucleoplasmin is a pentameric nuclear chaperone. The relation between the structure and the multifunctional aspects of the molecule has not yet been clearly established. In the course of analysing a C-terminally His-tagged recombinant version of the region equivalent to the trypsin resistant core (r-NP1...

journal_title：Archives of biochemistry and biophysics

authors： Arnan C,Prieto C,Chiva M,Salvany L,Ausió J,Subirana JA,Saperas N

更新日期：2005-05-15 00:00:00

abstract：:Recently we reported that oxidant tumor promoters can induce the oxidative modification of protein kinase C (PKC) resulting in either activation or inactivation of the kinase (R. Gopalakrishna and W. B. Anderson, Arch. Biochem. Biophys. 285, 382-387, 1991). Since retinoids previously have been shown to antagonize the ...

journal_title：Archives of biochemistry and biophysics

authors： Gundimeda U,Hara SK,Anderson WB,Gopalakrishna R

更新日期：1993-01-01 00:00:00

abstract：:The aim of this study was to investigate the effect of insulin resistance on glycogen concentration and glycogen synthase activity in the red and white gastrocnemius muscles and to determine whether the inverse relationship existing between glycogen concentration and enzyme activity is maintained in insulin resistant ...

journal_title：Archives of biochemistry and biophysics

authors： Coderre L,Vallega GA,Pilch PF,Chipkin SR

更新日期：2007-08-01 00:00:00

abstract：:Soybean Bowman-Birk protease inhibitor (BBI) is an inhibitor of serine proteases with two functional inhibitory domains of different specificities: one is specific for chymotrypsin-like proteases, the other for trypsin-like proteases. Chymase and tryptase are serine proteases which are stored in mast cell granules and...

journal_title：Archives of biochemistry and biophysics

authors： Ware JH,Wan XS,Rubin H,Schechter NM,Kennedy AR

更新日期：1997-08-01 00:00:00

abstract：:We have previously identified and characterized GSHPx-GI, which is a cellular selenium-dependent glutathione peroxidase (GSHPx) distinct from the classic GSHPx-1 and phospholipid hydroperoxide glutathione peroxidase (PHGPX). We have determined the level of GSHPx-GI mRNA expression in the rat gastrointestinal tract fro...

journal_title：Archives of biochemistry and biophysics

authors： Chu FF,Esworthy RS

更新日期：1995-11-10 00:00:00

abstract：:The effects of nitrous oxide inactivation of the vitamin B12-dependent enzyme, methionine synthetase, on the distribution of folic acid derivatives in rat bone marrow cells, kidney, brain, and liver were determined. Methionine synthetase activity was decreased by about 90% in bone marrow cells, kidney, and brain and b...

journal_title：Archives of biochemistry and biophysics

authors： Wilson SD,Horne DW

更新日期：1986-01-01 00:00:00

abstract：:Bcl-2 family proteins regulate apoptosis through their homo- and heterodimerization. By protein sequence analysis and structural comparison, we have identified a conserved hydrophobic core at the BH1 and BH2 domains of Bcl-2 family proteins. The hydrophobic core is stabilized by hydrophobic interactions among the resi...

journal_title：Archives of biochemistry and biophysics

authors： Feng Y,Zhang L,Hu T,Shen X,Ding J,Chen K,Jiang H,Liu D

更新日期：2009-04-01 00:00:00

abstract：:Prostaglandin E (PGE) stimulates resorption in bone. Since osteoblast-like osteosarcoma cells secrete PGE2, the possibility that osteoclasts were the major target for PGE was considered. To study this question, it was first established that in isolated bone cells enriched for either osteoclastic (OC) or osteoblastic (...

journal_title：Archives of biochemistry and biophysics

authors： Wong GL,Kocour BA

更新日期：1983-07-01 00:00:00

abstract：:The mechanism of the antiatherogenic activity of n-3 polyunsaturated fatty acids is not well understood. In these studies, we studied the metabolism of unsaturated fatty acids in murine macrophage-like J774A.1 cells. The major metabolic pathway of 18- and 20-carbon chain unsaturated fatty acids in these cells is the s...

journal_title：Archives of biochemistry and biophysics

authors： Shichiri G,Kinoshita M,Saeki Y

更新日期：1993-06-01 00:00:00

abstract：:The nuclear Ah receptor from mouse hepatoma (Hepa-1c1c9) cells is a 176-kDa multimeric protein which is stable under conditions of up to 1 M KCl. Under denaturing conditions, the Hepa-1 nuclear receptor can be dissociated into a ligand-binding subunit of Mr approximately 91,000. The identity of subunits that compose t...

journal_title：Archives of biochemistry and biophysics

authors： Prokipcak RD,Denison MS,Okey AB

更新日期：1990-12-01 00:00:00

abstract：:A monoclonal antibody specific for cytochrome P-450 1 that extensively (greater than 95%) inhibits the hepatic 21-hydroxylation of progesterone was used in a two-site immunoradiometric assay to estimate the concentration of cytochrome P-450 1 in microsomes prepared from 24 individual, untreated New Zealand White rabbi...

journal_title：Archives of biochemistry and biophysics

authors： Johnson EF,Griffin KJ

更新日期：1985-02-15 00:00:00

abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD, dioxin) produces many of its biological effects by binding to a soluble, intracellular protein (the Ah receptor (AhR]. The hepatic AhR, from a variety of species, is present in low salt cytosol as a form which sediments at 8-10 S. High salt (0.4 M KCL) dissociates the rat, gu...

journal_title：Archives of biochemistry and biophysics

authors： Denison MS,Vella LM

更新日期：1990-03-01 00:00:00

abstract：:African trypanosomes of the Trypanosoma brucei group are agents of disease in man and animals. They present unique biochemical characteristics such as the need for preformed purines and have extensive salvage mechanisms for nucleoside recovery. In this regard we have shown that trypanosomes have a dedicated transporte...

journal_title：Archives of biochemistry and biophysics

authors： Goldberg B,Rattendi D,Lloyd D,Yarlett N,Bacchi CJ

更新日期：1999-04-01 00:00:00

abstract：:To study the relationship between neutral aminopeptidase activity and hemoglobin accumulation in malaria parasites, we treated mice infected with Plasmodium berghei NYU-2 with chloroquine intraperitoneally in doses ranging from 0.3 to 3 micromol per 25 g mouse. Preparations of infected erythrocytes (normalized to repr...

journal_title：Archives of biochemistry and biophysics

authors： Fitch CD,Cai GZ,Chen YF,Ryerse JS

更新日期：2003-02-15 00:00:00

abstract：:Radiotherapy plays a limited role in the treatment of hepatocellular carcinoma (HCC) due to the development of resistance. Therefore, further investigation of underlying mechanisms involved in HCC radioresistance is warranted. Increasing evidence shows that long non-coding RNAs (linc-RNAs) are involved in the patholog...

journal_title：Archives of biochemistry and biophysics

authors： Chen Y,Shen Z,Zhi Y,Zhou H,Zhang K,Wang T,Feng B,Chen Y,Song H,Wang R,Chu X

更新日期：2018-05-01 00:00:00

abstract：:Immunological cross-reactivities between isolated subunits of the scorpion Androctonus australis (Aa) and of the horseshoe crab Limulus polyphemus (Lp) hemocyanins were studied using subunit-specific antibodies prepared through immunoadsorption to pure immobilized subunits. Rocket immunoelectrophoreses of the various ...

journal_title：Archives of biochemistry and biophysics

authors： Lamy J,Compin S,Lamy JN

更新日期：1983-06-01 00:00:00

abstract：:Vanadate-dependent oxidation of NADH by xanthine oxidase does not require the presence of xanthine and therefore is not due to cooxidation. Addition of NADH or xanthine had no effect on the oxidation of the other substrate. Oxidation of NADH was high at acid pH and oxidation of xanthine was high at alkaline pH. The sp...

journal_title：Archives of biochemistry and biophysics

authors： Khandke L,Gullapalli S,Patole MS,Ramasarma T

更新日期：1986-02-01 00:00:00

abstract：:Effects of free fatty acids on parameters of (Na+,K+)-ATPase regulation related to enzyme conformation were examined. Sensitivity to inhibition by free fatty acid increased as the number of double bonds increased. Free fatty acids reduced affinity for K+ or Na+ at their regulatory sites without altering apparent K+ af...

journal_title：Archives of biochemistry and biophysics

authors： Swann AC

更新日期：1984-09-01 00:00:00

abstract：:Cytochrome P450 reductase was purified to homogeneity from cell suspension cultures of the opium poppy Papaver somniferum, the enzyme was characterized (K(m) cytochrome c, 8.3 microM; K(m) NADPH, 4.2 microM; pH optimum, 8.0; M(r), 80 kDa), and the amino acid sequence of internal peptides was determined. Partial cDNA c...

journal_title：Archives of biochemistry and biophysics

authors： Rosco A,Pauli HH,Priesner W,Kutchan TM

更新日期：1997-12-15 00:00:00

abstract：:The mechanism by which dietary cholesterol regulates expression of the hepatic low-density lipoprotein (LDL) receptor was investigated. In a previous study (Arch. Biochem. Biophys. 325, 242-248, 1996), we demonstrated that dietary cholesterol reduces the rate of LDL receptor protein degradation without affecting stead...

journal_title：Archives of biochemistry and biophysics

authors： Lopez D,Ness GC

更新日期：1997-08-01 00:00:00