Analysis of the stability and function of nucleoplasmin through cysteine mutants.

abstract：:The plasma membrane of cells contains enzymes whose active sites face the external medium rather than the cytoplasm. The activities of these enzymes, referred to as ectoenzymes, can be measured using living cells. In this work we describe the ability of living promastigotes of Leishmania amazonensis to hydrolyze extra...

journal_title：Archives of biochemistry and biophysics

authors： Berrêdo-Pinho M,Peres-Sampaio CE,Chrispim PP,Belmont-Firpo R,Lemos AP,Martiny A,Vannier-Santos MA,Meyer-Fernandes JR

更新日期：2001-07-01 00:00:00

abstract：:Phospholipase C-gamma1, a substrate for many growth factor receptor and nonreceptor tyrosine kinases, produces second messenger molecules that are elements of signal transduction pathways related to cell proliferation. The influence of deletion mutations, which do not intrude on the domains required for catalytic func...

journal_title：Archives of biochemistry and biophysics

authors： Horstman DA,Chattopadhyay A,Carpenter G

更新日期：1999-01-01 00:00:00

abstract：:The metabolite 5-aminolevulinic acid (ALA) is an early committed intermediate in the biosynthetic pathway of heme and chlorophyll formation. In plants, 5-aminolevulinic acid is synthesized via a two-step pathway in which glutamyl-tRNA(Glu) is reduced by glutamyl-tRNA(Glu) reductase (GluTR) to glutamate 1-semialdehyde,...

journal_title：Archives of biochemistry and biophysics

authors： Loida PJ,Thompson RL,Walker DM,CaJacob CA

更新日期：1999-12-15 00:00:00

abstract：:An S-adenosyl-L-methionine:caffeoyl-CoA 3-O-methyltransferase was purified 82-fold from elicitor-induced parsley cell suspension cultures by ammonium sulfate fractionation, anionic exchange and hydrophobic interaction chromatographies, and chromatofocusing. The enzyme has an apparent pI of 5.7 and a molecular weight o...

journal_title：Archives of biochemistry and biophysics

authors： Pakusch AE,Kneusel RE,Matern U

更新日期：1989-06-01 00:00:00

abstract：:B4-2-1 cells (Lec15 cells) are Chinese hamster ovary cells deficient in mannosylphosphoryldolichol synthase activity. They synthesize the truncated lipid intermediate Man5GlcNAc2-P-P-dolichol rather than the Glc3Man9GlcNAc2-P-P-dolichol synthesized by wild-type cells. In this report we present evidence that these cell...

journal_title：Archives of biochemistry and biophysics

authors： Stoll J,Cacan R,Verbert A,Krag SS

更新日期：1992-12-01 00:00:00

abstract：:Arginase activity is elevated in livers of diabetic animals compared to controls and there is evidence that this is due in part to increased specific activity (activity/mg arginase protein). To investigate the molecular basis of this increased activity, the physicochemical and kinetic properties of hepatic arginase fr...

journal_title：Archives of biochemistry and biophysics

authors： Spolarics Z,Bond JS

更新日期：1989-11-01 00:00:00

abstract：:The retinal pigment epithelium (RPE)-specific protein RPE65 is the major protein of the RPE microsomal membrane fraction. Though RPE65 lacks transmembrane domains or signal peptide, detergents are required for its maximally effective solubilization in isotonic buffers. However, in 0.75-1.0 M KCl, RPE65 is as soluble w...

journal_title：Archives of biochemistry and biophysics

authors： Tsilou E,Hamel CP,Yu S,Redmond TM

更新日期：1997-10-01 00:00:00

abstract：:Bovine kidney mitochondrial extracts contain an inactive protamine kinase and an inactive casein kinase. The protamine kinase was activated by chromatography on poly(L-lysine)-agarose. Two forms of this soluble mitochondrial protamine kinase were separated by chromatography on protamine-agarose. Both forms were purifi...

journal_title：Archives of biochemistry and biophysics

authors： Damuni Z,Reed LJ

更新日期：1988-05-01 00:00:00

abstract：:By anion-exchange chromatography, the nitrogen fixing photosynthetic stem nodules and nonphotosynthetic root nodules of Sesbania rostrata are shown to contain the same seven major components of leghemoglobin (Lb), numbered LbI-LbVII in order of elution, although in different proportions. No novel component was found i...

journal_title：Archives of biochemistry and biophysics

authors： Bogusz D,Kortt AA,Appleby CA

更新日期：1987-04-01 00:00:00

abstract：:Diphtheria toxin (DT) induces the leakage of dipalmitoylphosphatidic acid (DPPA) membranes but not neutral dipalmitoylphosphatidylcholine (DPPC) membranes. Cholesterol incorporated into liposomes enhances the membrane leakage induced by DT in acidic DPPA membranes but not in neutral DPPC membranes. Membrane leakage wa...

journal_title：Archives of biochemistry and biophysics

authors： Lai CS,Kushnaryov V,Panz T,Basosi R

更新日期：1984-10-01 00:00:00

abstract：:Plant polyphenols are extremely diverse, due to the occurrence of several basic structures, numerous substitutions and, for some groups, of polymers (tannins). Plant polyphenol composition depends on the plant species and organ, with some molecules specific of particular plant families while others are ubiquitous. The...

journal_title：Archives of biochemistry and biophysics

authors： Tarascou I,Souquet JM,Mazauric JP,Carrillo S,Coq S,Canon F,Fulcrand H,Cheynier V

更新日期：2010-09-01 00:00:00

abstract：:Lipophosphoglycan (LPG) is the major surface glycoconjugate of Leishmania donovani promastigotes and is composed of a capped polymer of repeating PO4-6Gal(beta 1,4)Man alpha 1 disaccharide units linked via a phosphosaccharide core to a lyso-1-O-alkylphosphatidylinositol anchor. An exogenous acceptor composed of the gl...

journal_title：Archives of biochemistry and biophysics

authors： Carver MA,Turco SJ

更新日期：1992-06-01 00:00:00

abstract：:Peroxynitrite, a biological oxidant formed from the reaction of nitric oxide with the superoxide radical, is associated with many pathologies, including neurodegenerative diseases, such as multiple sclerosis (MS). Gout (hyperuricemic) and MS are almost mutually exclusive, and uric acid has therapeutic effects in mice ...

journal_title：Archives of biochemistry and biophysics

authors： Squadrito GL,Cueto R,Splenser AE,Valavanidis A,Zhang H,Uppu RM,Pryor WA

更新日期：2000-04-15 00:00:00

abstract：:Treatment with neuronal growth factor (NGF) results in the growth of neuronal processes by PC12 cells and a concomitant 70% increase in the area of the Golgi apparatus. To define the observed morphologic changes in biochemical terms, we investigated the effect of NGF treatment on some Golgi and lysosomal enzyme activi...

journal_title：Archives of biochemistry and biophysics

authors： Rhodes CH,Mezitis SG,Gonatas NK,Fleischer B

更新日期：1989-07-01 00:00:00

abstract：:The relative reduction potentials of a variety of carotenoids have been established by monitoring the reaction of carotenoid radical anion (CAR1(*-)) with another carotenoid (CAR2) in hexane and benzene. This order is consistent with the reactivities of the carotenoid radical anions with porphyrins and oxygen in hexan...

journal_title：Archives of biochemistry and biophysics

authors： Edge R,El-Agamey A,Land EJ,Navaratnam S,George Truscott T

更新日期：2007-02-15 00:00:00

abstract：:A catalase-related allene oxide synthase (cAOS) and true catalases that metabolize hydrogen peroxide have similar structure around the heme. One of the distal heme residues considered to help control catalysis is a highly conserved asparagine. Here we addressed the role of this residue in metabolism of the natural sub...

journal_title：Archives of biochemistry and biophysics

authors： Gao B,Boeglin WE,Brash AR

更新日期：2008-09-15 00:00:00

abstract：:A monoclonal antibody specific for cytochrome P-450 1 that extensively (greater than 95%) inhibits the hepatic 21-hydroxylation of progesterone was used in a two-site immunoradiometric assay to estimate the concentration of cytochrome P-450 1 in microsomes prepared from 24 individual, untreated New Zealand White rabbi...

journal_title：Archives of biochemistry and biophysics

authors： Johnson EF,Griffin KJ

更新日期：1985-02-15 00:00:00

abstract：:Ca2+ binding and internalization in sarcoplasmic reticulum ATPase can be investigated by the use of La3+ as a Ca2+ analog. Displacement kinetics of Ca2+ bound by La3+ in native vesicles is a slow biphasic process (k1 = 0.55 s-1 and k2 = 0.05 s-1) that is consistent with the existence of two Ca2+ binding populations wh...

journal_title：Archives of biochemistry and biophysics

authors： Fernandez-Belda F

更新日期：1988-12-01 00:00:00

abstract：:Since the hepatic LDL receptor is regarded as a major determinant of plasma LDL levels, the effect of diabetes on the expression of this receptor was examined in rat liver. Inducing diabetes with streptozotocin caused a significant reduction in hepatic LDL receptor mRNA levels in concert with an increase in serum chol...

journal_title：Archives of biochemistry and biophysics

authors： Niesen M,Bedi M,Lopez D

更新日期：2008-02-15 00:00:00

abstract：:The allylamine class of antifungal compounds are specific inhibitors of squalene epoxidase (SE). However, depending on their chemical structure, allylamine derivatives can be highly selective for either fungal or mammalian SEs. All allylamines tested previously, irrespective of their selectivity, inhibit fungal SEs in...

journal_title：Archives of biochemistry and biophysics

authors： Favre B,Ryder NS

更新日期：1997-04-15 00:00:00

abstract：:Prothymosin alpha, a polypeptide of 109 to 111 amino acid residues, contains the entire thymosin alpha 1 sequence (residues 1-28) at its amino terminal. Human peripheral blood monocytes incubated with prothymosin alpha release thymosin alpha 1 in the culture supernatants. In addition total RNA is found to increase. Th...

journal_title：Archives of biochemistry and biophysics

authors： Frillingos S,Seferiadis K,Papanastasiou M,Baxevanis CN,Frangou-Lazaridis M,Economou M,Papamichail M,Tsolas O

更新日期：1992-07-01 00:00:00

abstract：:delta 2-Isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase, which catalyzes the formation of isopentenyl-AMP from delta 2-isopentenylpyrophosphate and 5'AMP, was purified 6800-fold from the fruiting body of the cellular slime mold Dictyostelium discoideum using several separation procedures including 5'AMPo...

journal_title：Archives of biochemistry and biophysics

authors： Ihara M,Taya Y,Nishimura S,Tanaka Y

更新日期：1984-05-01 00:00:00

abstract：:Phosphate-dependent glutaminase is associated with the inner membrane of rat renal mitochondria. The orientation of this enzyme was characterized by comparing its sensitivity in isolated mitochondria and in mitoplasts to two membrane impermeable inhibitors. Mitoplasts were prepared by repeated swelling of mitochondria...

journal_title：Archives of biochemistry and biophysics

authors： Shapiro RA,Haser WG,Curthoys NP

更新日期：1985-11-15 00:00:00

abstract：:A new strategy for the functional reconstitution of membrane proteins is described. This approach introduces a new class of protein stabilizing agents--osmolytes--whose presence at high concentration (10-20%) during detergent solubilization prevents the inactivations that normally occur when proteins are extracted fro...

journal_title：Archives of biochemistry and biophysics

authors： Maloney PC,Ambudkar SV

更新日期：1989-02-15 00:00:00

abstract：:The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

journal_title：Archives of biochemistry and biophysics

authors： Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

更新日期：1999-03-15 00:00:00

abstract：:Recombinant ethanolamine ammonia-lyase from S. typhimurium has been overexpressed and purified in large quantities by a simple procedure. The molecular weight of the native enzyme is about 480 kDa, and it contains two active sites/molecule as shown by kinetic studies and by titration with CNCbl. Analysis by sodium dod...

journal_title：Archives of biochemistry and biophysics

authors： Faust LP,Babior BM

更新日期：1992-04-01 00:00:00

abstract：:This work was purposed to speculate the possible association of rs2910164hsa-miR-146a C>G gene single nucleotide polymorphism in the pathogenesis of schizophrenia and subsequently their relevance to neuro-inflammatory, vascular and oxidative stress pathways as acute ischemic stroke (AIS) risk factors in chronic schizo...

journal_title：Archives of biochemistry and biophysics

authors： Ibrahim RR,Amer RA,Abozeid AA,Elsharaby RM,Shafik NM

更新日期：2020-01-15 00:00:00

abstract：:The appearance of a high molecular weight gelatinolytic enzyme (230 kDa) correlated with cartilage collagen loss in chick embryonic tibias cultured with lipopolysaccharide. This 230 kDa enzyme was purified and its activity was measured on synthetic and natural substrates. The enzyme was activated by aminophenylmercuri...

journal_title：Archives of biochemistry and biophysics

authors： Patchigolla RK,Knudson W,Schmid TM

更新日期：2012-04-01 00:00:00

abstract：:Autophagy, a ubiquitous degradation pathway, is important for the survival and homeostasis of cells. Previous studies have demonstrated the role of autophagy in host defense against bacterial infection, but the importance of autophagy in the intestinal epithelium for the regulation of bacterial infection has not been ...

journal_title：Archives of biochemistry and biophysics

authors： Inoue J,Nishiumi S,Fujishima Y,Masuda A,Shiomi H,Yamamoto K,Nishida M,Azuma T,Yoshida M

更新日期：2012-05-01 00:00:00

abstract：:The single-stranded, DNA-dependent ATPase activity of purified recA protein was found to be inhibited competitively by ribose-modified analogs of ATP, 3'-O-anthraniloyl-ATP (Ant-ATP), and 3'-O-(N-methylanthraniloyl)-ATP (Mant-ATP). The Ki values for Ant-ATP and Mant-ATP were around 7 and 3 microM at pH 7.5, respective...

journal_title：Archives of biochemistry and biophysics

authors： Karasaki Y,Higashi K

更新日期：1984-09-01 00:00:00