Abstract:
:The interactions of Cu, Zn superoxide dismutase (SOD) with nitroxyl (NO-) and nitric oxide (NO), both of which are thought to be biologically significant, have been studied but remain undefined. Having previously noted that NO- can reduce Cu (II), Zn SOD aerobically, we now report that it also can do so anaerobically and that Cu, Zn SOD can catalyze the elimination of NO(-) in the absence of O2.NO- acts as a reductant of ferricytochrome c anaerobically, but in the presence of O2 causes the oxidation of ferrocytochrome c and NADPH. Equivalent fluxes of NO-, and NO + O2- were able to comparably oxidize NADPH, but the oxidation by NO + O2- was more than fivefold more sensitive to inhibition by Cu, Zn SOD than was the oxidation by NO-. Thus Cu, Zn SOD inhibited NADPH oxidation by NO- by a route independent of catalyzing the dismutation of O2. Plausible mechanisms for those observations are offered and rate constants are estimated.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Liochev SI,Fridovich Idoi
10.1016/S0003-9861(02)00074-7subject
Has Abstractpub_date
2002-06-15 00:00:00pages
166-71issue
2eissn
0003-9861issn
1096-0384pii
S0003-9861(02)00074-7journal_volume
402pub_type
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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更新日期:2020-09-30 00:00:00