Two phosphorylated forms of myosin in thrombin-stimulated platelets.

abstract：:Calmodulin is phosphorylated by the purified insulin receptor on tyrosine residues with a maximum stoichiometry of 1 mol phosphate/mol of calmodulin. Isolated tryptic phosphopeptides were sequenced by manual Edman degradation and demonstrated that calmodulin is equally phosphorylated on tyrosine 99 and tyrosine 138. P...

journal_title：Archives of biochemistry and biophysics

authors： Williams JP,Jo H,Sacks DB,Crimmins DL,Thoma RS,Hunnicutt RE,Radding W,Sharma RK,McDonald JM

更新日期：1994-11-15 00:00:00

abstract：:A neutral proteolytic activity that converts human Big endothelin-1 (Big Et-1) to endothelin-1 has been identified from a human endothelial hybrid cell line, EAHY 926. This enzyme is an integral membrane protein and cofractionates with other enzymes typically found in the plasma membrane. The activity has been solubil...

journal_title：Archives of biochemistry and biophysics

authors： Waxman L,Doshi KP,Gaul SL,Wang S,Bednar RA,Stern AM

更新日期：1994-01-01 00:00:00

abstract：:Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, stimulates both the cellular uptake of radiolabeled choline and the activity of CTP:phosphocholine cytidylyltransferase resulting in increased incorporation of choline into phosphatidylcholine (PtdCho). Because of these multiple actions, it...

journal_title：Archives of biochemistry and biophysics

authors： Kiss Z

更新日期：1996-11-01 00:00:00

abstract：:Nitroxides stable radicals are unreactive toward most diamagnetic molecules, but readily undergo one-electron redox reactions with paramagnetic species such as free radicals and transition metals, thus serving as cell-permeable antioxidants. The cytotoxicity of juglone (5-hydroxy-1,4-naphthoquinone), like that of othe...

journal_title：Archives of biochemistry and biophysics

authors： Zhang R,Hirsch O,Mohsen M,Samuni A

更新日期：1994-08-01 00:00:00

abstract：:The partial degradation of proteoglycan aggregate by human leucocyte elastase yielded products that banded with Mr 190,000, 140,000, 88,000, and 71,000 when analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis. Analysis of these bands revealed that the 190,000- and 140,000-Da bands contained chondro...

journal_title：Archives of biochemistry and biophysics

authors： Mok MT,Ilic MZ,Handley CJ,Robinson HC

更新日期：1992-02-01 00:00:00

abstract：:Cytochrome P4502E (P4502E), the major ethanol-inducible P450 metabolizes ethanol to acetaldehyde and bioactivates procarcinogens to ultimate carcinogens. Metabolism of ethanol to acetaldehyde in the brain could be deleterious since it can react with cytoskeletal proteins, forming adducts. In the present study, rats we...

journal_title：Archives of biochemistry and biophysics

authors： Upadhya SC,Tirumalai PS,Boyd MR,Mori T,Ravindranath V

更新日期：2000-01-01 00:00:00

abstract：:Paxilline, an indole alkaloid mycotoxin from Penicillium paxilli, is an inhibitor of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA). Paxilline inhibited differing isoforms of SERCA with IC50s between 5 and 50 microM. It inhibited more potently the purified Ca2+ ATPase activity from skeletal muscle with an IC50 of...

journal_title：Archives of biochemistry and biophysics

authors： Bilmen JG,Wootton LL,Michelangeli F

更新日期：2002-10-01 00:00:00

abstract：:2-Isopropenyl-2-methyladamantane (2-PMADA) and 3-isopropenyl-3-methyldiamantane (3-PMDIA) showed potent and selective inhibition of cytochrome P450 (CYP) 2B6-mediated reactions with K(i) values of 5.27 and 2.17 microM, respectively. No effect on activities of other human CYP was found even at concentrations 100-fold h...

journal_title：Archives of biochemistry and biophysics

authors： Stiborová M,Borek-Dohalská L,Hodek P,Mráz J,Frei E

更新日期：2002-07-01 00:00:00

abstract：:A detailed knowledge about the substrate specificities of the heparin lyases is necessary when using these enzymes as tools for elucidating the sequence of heparin and heparan sulfate. The substrate specificity of heparin lyases I, II, and III have been profiled with structurally defined, heparin-derived oligosacchari...

journal_title：Archives of biochemistry and biophysics

authors： Desai UR,Wang HM,Linhardt RJ

更新日期：1993-11-01 00:00:00

abstract：:Chaperonins are multi-subunit complexes that enhance the efficiency of protein-folding reactions by capturing protein substrates in their central cavities. They occur in all prokaryotic and eukaryotic cell types and, alone amongst molecular chaperones, chaperonin knockouts are always lethal. Chaperonins come in two fo...

journal_title：Archives of biochemistry and biophysics

authors： Bigotti MG,Clarke AR

更新日期：2008-06-15 00:00:00

abstract：:Cancer osaka thyroid (COT), a human MAP 3 K, is essential for lipopolysaccharide activation of the Erk MAPK cascade in macrophages. COT 30--467 is insoluble, whereas low levels of COT 30--397 can be expressed, but this protein is unstable. However, both COT 30--467 and COT 30--397 are expressed in a soluble and stable...

journal_title：Archives of biochemistry and biophysics

authors： Jia Y,Quinn CM,Bump NJ,Clark KM,Clabbers A,Hardman J,Gagnon A,Kamens J,Tomlinson MJ,Wishart N,Allen H

更新日期：2005-09-01 00:00:00

abstract：:The amino acid L-lysine is synthesized in Saccharomyces cerevisiae via the α-aminoadipate pathway. An as yet unidentified PLP-containing aminotransferase is thought to catalyze the formation of α-aminoadipate from α-ketoadipate in the L-lysine biosynthetic pathway that could be the yeast Aro8 gene product. A screen of...

journal_title：Archives of biochemistry and biophysics

authors： Karsten WE,Reyes ZL,Bobyk KD,Cook PF,Chooback L

更新日期：2011-12-01 00:00:00

abstract：:Lipophosphoglycan (LPG) is the major surface glycoconjugate of Leishmania donovani promastigotes and is composed of a capped polymer of repeating PO4-6Gal(beta 1,4)Man alpha 1 disaccharide units linked via a phosphosaccharide core to a lyso-1-O-alkylphosphatidylinositol anchor. An exogenous acceptor composed of the gl...

journal_title：Archives of biochemistry and biophysics

authors： Carver MA,Turco SJ

更新日期：1992-06-01 00:00:00

abstract：:Xenopus laevis nucleoplasmin is a pentameric nuclear chaperone. The relation between the structure and the multifunctional aspects of the molecule has not yet been clearly established. In the course of analysing a C-terminally His-tagged recombinant version of the region equivalent to the trypsin resistant core (r-NP1...

journal_title：Archives of biochemistry and biophysics

authors： Arnan C,Prieto C,Chiva M,Salvany L,Ausió J,Subirana JA,Saperas N

更新日期：2005-05-15 00:00:00

abstract：:Two peptides are specified when the noncoding DNA strand is read in the 5' to 3', or the 3' to 5' direction, and both peptides form strong complexes with the natural peptide, as found by J. E. Blalock and K. L. Bost with ACTH [1986) Biochem. J. 234, 679-683). We report here that strong hydropathic complementarity (pai...

journal_title：Archives of biochemistry and biophysics

authors： Markus G,Tritsch GL,Parthasarathy R

更新日期：1989-08-01 00:00:00

abstract：:CYP2D15 is the canine ortholog of human CYP2D6, the human CYP2D isoform involved in the metabolism of drugs such as antiarhythmics, adrenoceptor antagonists, and tricyclic antidepressants. Similar to human, canine CYP2D15 is expressed in the liver, with detectable levels in several other tissues. Three different CYP2D...

journal_title：Archives of biochemistry and biophysics

authors： Roussel F,Duignan DB,Lawton MP,Obach RS,Strick CA,Tweedie DJ

更新日期：1998-09-01 00:00:00

abstract：:Altered iso-1- and iso-2-cytochromes c, with certain amino acid replacements, occur at diminished levels due to degradation in the yeast Saccharomyces cerevisiae. A subclass of the labile isocytochromes c are significantly protected from degradation by the presence of cytochromes a.a3 and c1, the physiological partner...

journal_title：Archives of biochemistry and biophysics

authors： Pearce DA,Sherman F

更新日期：1998-04-01 00:00:00

abstract：:The oxidation of quercetin by horseradish peroxidase/H(2)O(2) was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the absence of GSH revealed formation of at least 20 different products, a result in line with other studies reporting the ...

journal_title：Archives of biochemistry and biophysics

authors： Awad HM,Boersma MG,Vervoort J,Rietjens IM

更新日期：2000-06-15 00:00:00

abstract：:Regional hyperthermia has potential for human cancer treatment, particularly in combination with systemic chemotherapy or radiotherapy. The mechanisms involved in heat-induced cell killing are currently unknown. Hyperthermia may increase oxidative stress in cells, and thus, oxidative stress could have a role in the me...

journal_title：Archives of biochemistry and biophysics

authors： Lord-Fontaine S,Averill DA

更新日期：1999-03-15 00:00:00

abstract：:The earthworm-derived chemoattractant ES20 interacts with its G-protein-coupled receptors on the plasma membrane of vomeronasal (VN) sensory neurons of garter snakes, resulting in an increase in inositol trisphosphate [J. Biol. Chem. 269 (1994) 16867] and a rapid phosphorylation of the membrane-bound proteins, p42/44 ...

journal_title：Archives of biochemistry and biophysics

authors： Wang D,Liu W,Liu J,Chen P,Quan W,Halpern M

更新日期：2002-12-15 00:00:00

abstract：:Excessive proliferation, migration and dedifferentiation of vascular smooth muscle cells (VSMCs) are the center of intimal formation during in-stent restenosis and vein graft disease. Paeoniflorin-6'-O-benzene sulfonate (CP-25) is known to suppress inflammation and atherogenesis. However, the potential effect of CP-25...

journal_title：Archives of biochemistry and biophysics

authors： Zhang J,Liu Y,Long M,Li J,Zhao W,Su Q

更新日期：2020-11-15 00:00:00

abstract：:Cofactor and tryptophan accessibility of the 65-kDa form of rat brain glutamate decarboxylase (GAD) was investigated by fluorescence quenching measurements using acrylamide, I-, and Cs+ as the quenchers. Trp residues were partially exposed to solvent. I- was less able and Cs+ was more able to quench the fluorescence o...

journal_title：Archives of biochemistry and biophysics

authors： Rust E,Martin DL,Chen CH

更新日期：2001-08-15 00:00:00

abstract：:Prothymosin alpha, a polypeptide of 109 to 111 amino acid residues, contains the entire thymosin alpha 1 sequence (residues 1-28) at its amino terminal. Human peripheral blood monocytes incubated with prothymosin alpha release thymosin alpha 1 in the culture supernatants. In addition total RNA is found to increase. Th...

journal_title：Archives of biochemistry and biophysics

authors： Frillingos S,Seferiadis K,Papanastasiou M,Baxevanis CN,Frangou-Lazaridis M,Economou M,Papamichail M,Tsolas O

更新日期：1992-07-01 00:00:00

abstract：:Treatment of streptozotocin (ST), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) resulted in destroying insulin-secreting beta-cells of pancreatic islets and impairment of islet glucose oxidation and glucose-induced insulin secretion. IL-1beta and TNF-alpha inhibited insulin release and gluc...

journal_title：Archives of biochemistry and biophysics

authors： Park C,Kim JR,Shim JK,Kang BS,Park YG,Nam KS,Lee YC,Kim CH

更新日期：1999-02-15 00:00:00

abstract：:Secreted frizzled-related protein (sFRP)-1 is a Wnt antagonist that inhibits breast carcinoma cell motility, whereas the secreted glycoprotein thrombospondin-1 stimulates adhesion and motility of the same cells. We examined whether thrombospondin-1 and sFRP-1 interact directly or indirectly to modulate cell behavior. ...

journal_title：Archives of biochemistry and biophysics

authors： Martin-Manso G,Calzada MJ,Chuman Y,Sipes JM,Xavier CP,Wolf V,Kuznetsova SA,Rubin JS,Roberts DD

更新日期：2011-05-15 00:00:00

abstract：:Initiation of lipid peroxidation in the inner mitochondrial membrane was investigated using respiratory substrates and inhibitors and various iron chelates. An iron chelate was required for initiation of lipid peroxidation in the presence of either NADH or NADPH. The two nicotinamide nucleotides exhibited different ac...

journal_title：Archives of biochemistry and biophysics

authors： Glinn M,Ernster L,Lee CP

更新日期：1991-10-01 00:00:00

abstract：:The role of the inhibitor of apoptosis (IAP) family members in tumor necrosis factor-α (TNF-α)-induced apoptosis of human gastric cancer MKN28 cells was explored. TNF-α induced up-regulation of cIAP2, whereas cycloheximide (CHX) induced down-regulation of XIAP and survivin. Degradation of cIAP1 and XIAP, but not survi...

journal_title：Archives of biochemistry and biophysics

authors： Kitagawa M,Shiozaki A,Ichikawa D,Nakashima S,Kosuga T,Konishi H,Komatsu S,Fujiwara H,Okamoto K,Otsuji E

更新日期：2015-01-15 00:00:00

abstract：:Progress of mutagenesis studies on the relation of the structure of Na+/K+-ATPase to its reaction mechanism has been impeded by the paucity of information on the properties of small amounts of impure recombinant enzyme obtained in the currently available expression systems, and the uncertainty of whether expression in...

journal_title：Archives of biochemistry and biophysics

authors： Xie Z,Wang Y,Liu G,Zolotarjova N,Periyasamy SM,Askari A

更新日期：1996-06-01 00:00:00

abstract：:The steric course of methyl group transfer catalyzed by two DNA methylases, HhaI methylase, a DNA (cytosine-5)-methyltransferase, and EcoRI methylase, which methylates at N6 of adenosine, has been studied with (methyl-R)- and (methyl-S)-[methyl-2H1,3H]adenosylmethionine as the methyl donor, using as substrates poly-d(...

journal_title：Archives of biochemistry and biophysics

authors： Ho DK,Wu JC,Santi DV,Floss HG

更新日期：1991-02-01 00:00:00

abstract：:Mutations in MYBPC3, the gene encoding cardiac myosin binding protein C (cMyBP-C), are a major cause of hypertrophic cardiomyopathy (HCM). While most mutations encode premature stop codons, missense mutations causing single amino acid substitutions are also common. Here we investigated effects of a single proline for ...

journal_title：Archives of biochemistry and biophysics

authors： van Dijk SJ,Bezold Kooiker K,Mazzalupo S,Yang Y,Kostyukova AS,Mustacich DJ,Hoye ER,Stern JA,Kittleson MD,Harris SP

更新日期：2016-07-01 00:00:00