The amino acid sequence of the pancreatic islet mitochondrial glycerol phosphate dehydrogenase is not unique and the enzyme is not thyroid or glucose responsive.

abstract：:A series of peptidyl alpha-keto esters, alpha-keto amides, alpha-keto acids, and alpha-diketones were synthesized which reversibly inhibit papain and cathepsin B. Methyl 3-(N-benzyloxycarbonyl-L-phenylalanyl)amino-2-oxopropionate (a dicarbonyl compound) inhibits papain with a Ki value of 1 microM, whereas the Ki of 3-...

journal_title：Archives of biochemistry and biophysics

authors： Hu LY,Abeles RH

更新日期：1990-09-01 00:00:00

abstract：:The Ah receptor mediates the induction of cytochrome P450 1A1 (CYP1A1) and toxicities of 2,3,7,8tetrachlorodibanzo-p-dioxin (TCDD). It has been detected in tissues of many species and in murine and human hepatoma lines. We show that the human hepatoma line SK-Hep-1 has cytosolic Ah receptor detectable by specific bind...

journal_title：Archives of biochemistry and biophysics

authors： Roberts EA,Harper PA,Wong JM,Wang Y,Yang S

更新日期：2000-12-01 00:00:00

abstract：:The cDNA probe H-9, originally constructed to recognize a portion of the mRNA for lapine synovial collagenase, also hybridized with a RNA of the same size (approximately 2.0 kb) isolated from activated lapine articular chondrocytes. Primary, monolayer cultures of lapine articular chondrocytes did not contain detectabl...

journal_title：Archives of biochemistry and biophysics

authors： Lin CW,Phillips SL,Brinckerhoff CE,Georgescu HI,Bandara G,Evans CH

更新日期：1988-07-01 00:00:00

abstract：:Two different fractions were present in crystalline bovine liver catalase, and could be resolved using dye-ligand affinity chromatography with Red-A Matrex gel containing Procion HE 3B. The major part (alpha) was not adsorbed on this gel. The second fraction (beta) was firmly adsorbed to the gel, and could be eluted e...

journal_title：Archives of biochemistry and biophysics

authors： Jouve HM,Pelmont J,Gaillard J

更新日期：1986-07-01 00:00:00

abstract：:The hydrolysis of 32 X-phenyl-N-methanesulfonyl glycinates by papain was investigated. It was found that the variation in the Michaelis constants could be rationalized by the following correlation equation: log 1/Km = 0.61 pi '3 + 0.46 MR4 + 0.55 sigma + 2.00 with a correlation coefficient of 0.945. In this expression...

journal_title：Archives of biochemistry and biophysics

authors： Carotti A,Smith RN,Wong S,Hansch C,Blaney JM,Langridge R

更新日期：1984-02-15 00:00:00

abstract：:Kinetic analysis has shown that dimethylnitrosamine, dipropylnitrosamine, dibutylnitrosamine, and diphenylnitrosamine initially act as reversible competitive inhibitors with respect to the substrate, acetylthiocholine chloride. The inhibitor constants Ki vary from 21-30 microM for the aliphatic nitrosamines to 8.2 mic...

journal_title：Archives of biochemistry and biophysics

authors： Shuttleworth B,de Villiers DL,Whiteley CG

更新日期：1990-06-01 00:00:00

abstract：:Two recombinant sesquiterpene synthases from grand fir, delta-selinene synthase and gamma-humulene synthase, each produce more than 30 sesquiterpene olefins from the acyclic precursor farnesyl diphosphate. These enzymes contain a pair of DDxxD motifs, on opposite lips of the presumptive active site, which are thought ...

journal_title：Archives of biochemistry and biophysics

authors： Little DB,Croteau RB

更新日期：2002-06-01 00:00:00

abstract：:Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (SceADH) binds NAD+ and NADH less tightly and turns over substrates more rapidly than does horse (Equus caballus) liver alcohol dehydrogenase E isoenzyme (EcaADH), and neither enzyme uses NADP efficiently. Amino acid residues in the proposed adenylate binding po...

journal_title：Archives of biochemistry and biophysics

authors： Fan F,Plapp BV

更新日期：1999-07-15 00:00:00

abstract：:The C6.5 endoglucanase from Bacillus subtilis catalyzes the hydrolyses of beta-glucans. This enzyme, which is also produced by many ruminant microbes, is not part of the normal digestive repertoire of monogastric animals. We have generated transgenic mice which express the C6.5 endoglucanase gene specifically in the p...

journal_title：Archives of biochemistry and biophysics

authors： Zhang JX,Meidinger R,Forsberg CW,Krell PJ,Phillips JP

更新日期：1999-07-15 00:00:00

abstract：:Cutaneous malignant melanomas originate primarily within epidermal melanocytic cells. Melanoma cells share many characteristics with melanocyte precursors, suggesting that melanoma cells utilize the developmental programs of their normal counterpart for their own progression. The pigmentation system provides an advant...

journal_title：Archives of biochemistry and biophysics

authors： Liu J,Fukunaga-Kalabis M,Li L,Herlyn M

更新日期：2014-12-01 00:00:00

abstract：:In vitro phosphorylation of purified spectrin dimer was studied in the presence of Ca2+-calmodulin (CaM). CaM inhibited autophosphorylation of the beta subunit of spectrin. The inhibitory effect (65% at a 32-fold molar excess) appeared to be due to a weak interaction of CaM with spectrin. CaM was similarly effective i...

journal_title：Archives of biochemistry and biophysics

authors： Maretzki D,Lutz HU

更新日期：1986-04-01 00:00:00

abstract：:A ganglioside GM1-specific alpha 1-->2fucosyltransferase is induced during the early stages of chemical carcinogenesis with N-2-acetylaminofluorene (AAF) in rat liver hepatocytes. The induction of this enzyme gives rise to the expression of a fucose-containing ganglioside with the same determinant structure as blood g...

journal_title：Archives of biochemistry and biophysics

authors： Sherwood AL,Holmes EH

更新日期：1998-07-15 00:00:00

abstract：:Bovine liver glutamate dehydrogenase reacts covalently with 2-(4-bromo-2,3-dioxobutylthio)adenosine 5'-monophosphate (2-BDB-TAMP) with incorporation of 1 mol reagent/mol enzyme subunit and loss of one of the two ADP sites of native enzyme [S. P. Batra and R. F. Colman, J. Biol. Chem. 261, 15565-15571 (1986)]. Incorpor...

journal_title：Archives of biochemistry and biophysics

authors： Batra SP,Lark RH,Colman RF

更新日期：1989-04-01 00:00:00

abstract：:The glutamylation of methotrexate has been evaluated in H35 hepatoma cells in vitro as a function of the conditions of culture. Glutamylation yields methotrexate polyglutamate with two to five additional glutamate residues and is a saturable process. The rate of glutamylation increases little above 10 microM extracell...

journal_title：Archives of biochemistry and biophysics

authors： Nimec Z,Galivan J

更新日期：1983-10-15 00:00:00

abstract：:A monoclonal antibody specific for cytochrome P-450 1 that extensively (greater than 95%) inhibits the hepatic 21-hydroxylation of progesterone was used in a two-site immunoradiometric assay to estimate the concentration of cytochrome P-450 1 in microsomes prepared from 24 individual, untreated New Zealand White rabbi...

journal_title：Archives of biochemistry and biophysics

authors： Johnson EF,Griffin KJ

更新日期：1985-02-15 00:00:00

abstract：:The excimer fluorescence of pyrene iodoacetamide-labeled tropomyosin bound to actin is a probe of the regulatory state change of the muscle thin filament associated with the cooperative binding of myosin subfragment 1 (S1) (Y. Ishii and S. S. Lehrer, Biochemistry 29, 1160, 1990). In this study, the excimer fluorescenc...

journal_title：Archives of biochemistry and biophysics

authors： Ishii Y,Lehrer SS

更新日期：1993-08-15 00:00:00

abstract：:We evaluated the expression and activity of rat 12-lipoxygenase (LO) in rat intestinal epithelial (RIE) cells during apoptosis and cell differentiation. Sodium butyrate (NaBT) treatment induced wild-type RIE (W-RIE) cells to undergo differentiation and apoptosis. Alkaline phosphatase (ALP) activity, a marker of cell d...

journal_title：Archives of biochemistry and biophysics

authors： Kamitani H,Ikawa H,Hsi LC,Watanabe T,DuBois RN,Eling TE

更新日期：1999-08-01 00:00:00

abstract：:The photooxidizing xanthene dye rose bengal is shown to induce rapid Ca2+ release from skeletal muscle sarcoplasmic reticulum (SR) vesicles. In the presence of light, nanomolar concentrations of rose bengal increase the Ca2+ permeability of the SR and stimulate the production of singlet oxygen (1O2). In the absence of...

journal_title：Archives of biochemistry and biophysics

authors： Stuart J,Pessah IN,Favero TG,Abramson JJ

更新日期：1992-02-01 00:00:00

abstract：:Eleven hybridoma clones which secrete monoclonal antibodies against purified rat liver alcohol dehydrogenase (EC 1.1.1.1) were isolated. Antibodies (R-1-R-11) were identified by their ability to bind to immobilized pure alcohol dehydrogenase in an enzyme-linked immunoadsorbent assay, in which antibody R-9 showed the h...

journal_title：Archives of biochemistry and biophysics

authors： Lad PJ,Schenk DB,Leffert HL

更新日期：1984-12-01 00:00:00

abstract：:A neutral proteolytic activity that converts human Big endothelin-1 (Big Et-1) to endothelin-1 has been identified from a human endothelial hybrid cell line, EAHY 926. This enzyme is an integral membrane protein and cofractionates with other enzymes typically found in the plasma membrane. The activity has been solubil...

journal_title：Archives of biochemistry and biophysics

authors： Waxman L,Doshi KP,Gaul SL,Wang S,Bednar RA,Stern AM

更新日期：1994-01-01 00:00:00

abstract：:Ovalbumin extracted from oviduct slices incubated with [35S]methionine or [2-3]mannose contained two biosynthetic intermediates, OE and OF (Y. Kato, H. Iwase, and K. Hotta, 1984, J. Biochem. (Tokyo) 95, 455-463). In the present study, these intermediates are further characterized. The 3H dpm/35S dpm ratio of OF labele...

journal_title：Archives of biochemistry and biophysics

authors： Kato Y,Iwase H,Hotta K

更新日期：1986-02-01 00:00:00

abstract：:This study characterizes the structural and functional significance of sulfhydryl residues in human plasma heparin cofactor II (HCII). For quantification of sulfhydryl groups, the extinction coefficient of HCII was redetermined and found to be 0.593 ml mg-1 cm-1 using second-derivative spectroscopy and multicomponent ...

journal_title：Archives of biochemistry and biophysics

authors： Church FC,Meade JB,Pratt CW

更新日期：1987-12-01 00:00:00

abstract：:The nature and role of intermediates have been the subject of much heated debate in the field of protein folding. Historically, intermediates were viewed as essential stepping-stones that guide a protein through the folding process to the native state. However, with the experimental identification of numerous small pr...

journal_title：Archives of biochemistry and biophysics

authors： Tsytlonok M,Itzhaki LS

更新日期：2013-03-01 00:00:00

abstract：:Tetrahydro-dUMP, an analog of the putative transition state in aminohydrolysis of deoxycytidine monophosphate (dCMP) inhibits the allosteric enzyme deoxycytidylate aminohydrolase with high affinity. The inhibition is reversible, and its kinetics is consistent with the analog binding at the substrate site only to one a...

journal_title：Archives of biochemistry and biophysics

authors： Nucci R,Febbraio F,Piccialli G,de Napoli L,Vaccaro C,Rossi M,Whitehead EP

更新日期：1994-04-01 00:00:00

abstract：:The objective of this study was to determine if oxidative stress/damage is a possible causal factor in the senescence-related loss of brain functions in the mouse. If such a relationship indeed existed, it was expected that oxidative protein damage would increase with age within regions of the brain associated with se...

journal_title：Archives of biochemistry and biophysics

authors： Dubey A,Forster MJ,Lal H,Sohal RS

更新日期：1996-09-01 00:00:00

abstract：:Flavopiridol has been shown to induce cell cycle arrest and apoptosis in various tumor cells in vitro and in vivo. Using immobilized flavopiridol, we identified glycogen phosphorylases (GP) from liver and brain as flavopiridol binding proteins from HeLa cell extract. Purified rabbit muscle GP also bound to the flavopi...

journal_title：Archives of biochemistry and biophysics

authors： Kaiser A,Nishi K,Gorin FA,Walsh DA,Bradbury EM,Schnier JB

更新日期：2001-02-15 00:00:00

abstract：:Extracellular ATP is known to increase the membrane permeability of a variety of cells. Addition of ATP to human leukemic lymphocytes loaded with the Ca2+ indicator, fura-2, induced a rise in cytosolic Ca2+ concentration which was attenuated or absent in NaCl media compared with KCl, choline Cl, or NMG Cl media. In co...

journal_title：Archives of biochemistry and biophysics

authors： Wiley JS,Chen R,Wiley MJ,Jamieson GP

更新日期：1992-02-01 00:00:00

abstract：:Diastolic dysfunction refers to an impaired relaxation and an abnormality in ventricular blood filling during diastole while systolic function is preserved. Cardiac myofibril hypersensitivity to Ca(2+) is a major factor that causes impaired relaxation of myocardial cells. The present study investigates the effect of t...

journal_title：Archives of biochemistry and biophysics

authors： Zhang L,Nan C,Chen Y,Tian J,Jean-Charles PY,Getfield C,Wang X,Huang X

更新日期：2015-05-01 00:00:00

abstract：:The biosynthesis of one riboflavin molecule requires one molecule of GTP and two molecules of ribulose 5-phosphate as substrates. GTP is hydrolytically opened, converted into 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione by a sequence of deamination, side chain reduction and dephosphorylation. Condensation with 3,...

journal_title：Archives of biochemistry and biophysics

authors： Fischer M,Bacher A

更新日期：2008-06-15 00:00:00

abstract：:There is dose-dependent Cd(2+)-evoked swelling of isolated rat liver mitochondria energized by complex I, II, or IV respiratory substrates in sucrose medium in the absence of added Ca(2+) and P(i), which is prevented by Sr(2+). Permeability transition effectors (ADP, CsA, EGTA, RR, DTT, ATR, P(i), and Ca(2+)) affect i...

journal_title：Archives of biochemistry and biophysics

authors： Belyaeva EA,Glazunov VV,Korotkov SM

更新日期：2002-09-15 00:00:00