Interaction of the high-affinity inhibitor tetrahydro-dUMP with the allosteric enzyme deoxycytidylate aminohydrolase.

abstract：:The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

journal_title：Archives of biochemistry and biophysics

authors： Salvador JM,Mata AM

更新日期：1998-03-15 00:00:00

abstract：:In the present study steered molecular dynamics simulations were applied to investigate the unbinding process of the complex of human carbonic anhydrase with the natural HCO3(-) and promiscuous H2NCOHN(-) products. This process is crucial for restoring the catalytic cycle of the enzyme. This investigation set out to g...

journal_title：Archives of biochemistry and biophysics

authors： Piazzetta P,Marino T,Russo N

更新日期：2015-09-15 00:00:00

abstract：:The stability of the Glossoscolex paulistus hemoglobin (HbGp), in two iron oxidation states (and three forms), as monitored by optical absorption, fluorescence emission and circular dichroism (CD) spectroscopies, in the presence of the chaotropic agent urea, is studied. HbGp oligomeric dissociation, denaturation and i...

journal_title：Archives of biochemistry and biophysics

authors： Carvalho FA,Santiago PS,Tabak M

更新日期：2012-03-01 00:00:00

abstract：:Phorbol esters induce rapid downregulation of PAF receptors on human neutrophils. The molecular mechanism responsible for this observation has not been elucidated. In the present study, treatment of neutrophils with PMA (10(-7) M) for 10 min caused a complete loss of PAF receptors from the cell surface as determined b...

journal_title：Archives of biochemistry and biophysics

authors： Zhou W,Olson MS

更新日期：1994-08-15 00:00:00

abstract：:A peptide containing 43 amino acid residues, rich in glutamic acid and lysine, was originally isolated from calf thymus and designated thymosin beta 4 [T.L.K. Low, S. -K. Hu, and A. L. Goldstein (1981) Proc. Nat. Acad. Sci. USA 78, 1162-1166]. However, thymosin beta 4 was also shown to be present in other tissues of r...

journal_title：Archives of biochemistry and biophysics

authors： Erickson-Viitanen S,Ruggieri S,Natalini P,Horecker BL

更新日期：1983-03-01 00:00:00

abstract：:Sequence analyses of the C-terminal membrane intercalative region of the rat cytochrome b(5) indicated that this domain has, in addition to a signal sequence, a combined element of the classic stop-transfer sequence typically found in a variety of transmembrane proteins. Such bitopic protein arrangements arise by tand...

journal_title：Archives of biochemistry and biophysics

authors： Kaderbhai MA,Morgan R,Kaderbhai NN

更新日期：2003-04-15 00:00:00

abstract：:The Arabidopsis thaliana genome contains at least 32 terpenoid synthase (AtTPS) genes [Aubourg et al., Mol. Genet. Genom. 267 (2002) 730] a few of which have recently been characterized. Based on hierarchical cluster analysis of AtTPS gene expression, measured by microarray profiling and validated with published expre...

journal_title：Archives of biochemistry and biophysics

authors： Ro DK,Ehlting J,Keeling CI,Lin R,Mattheus N,Bohlmann J

更新日期：2006-04-15 00:00:00

abstract：:Mammalian multidrug and toxic compound extrusion 1 (MATE1) are polyspecific H+-coupled exporters of organic cations (OCs) and responsible for excretion of metabolic waste products and xenobiotics. Here, we report a novel variant of mouse MATE1, mMATE1b, that has a long carboxyl terminal hydrophobic tail homologous to ...

journal_title：Archives of biochemistry and biophysics

authors： Kobara A,Hiasa M,Matsumoto T,Otsuka M,Omote H,Moriyama Y

更新日期：2008-01-15 00:00:00

abstract：:Histidinol dehydrogenase (EC 1.1.1.23) activity was determined in several plant species and in cultured plant cell lines. The enzyme was purified from cabbage (Brassica oleracea) to apparent homogeneity. To render complete purification, a new, specific histidinol-Sepharose 4B affinity chromatography was developed. The...

journal_title：Archives of biochemistry and biophysics

authors： Nagai A,Scheidegger A

更新日期：1991-01-01 00:00:00

abstract：:The reaction by which the antioxidant capacity of alpha-tocopherol is sustained by recycling of the alpha-tocopheroxyl radical in membranes or lipoproteins by aqueous ascorbate has been studied by laser flash photolysis in model micellar and membrane systems. In bilayers of dimyristoylphosphatidylcholine at 35 degrees...

journal_title：Archives of biochemistry and biophysics

authors： Bisby RH,Parker AW

更新日期：1995-02-20 00:00:00

abstract：:Non-enveloped viruses require membrane-penetrating peptides for gaining entry inside the cytoplasm of host cells during the early stages of infection. Although several such peptides have been identified as essential components for non-enveloped virus entry, the molecular mechanism of membrane destabilization by these ...

journal_title：Archives of biochemistry and biophysics

authors： Nair P,Dey D,Borkotoky S,Shukla A,Banerjee M

更新日期：2019-12-15 00:00:00

abstract：BACKGROUND:Proteomics is expected to provide novel insights in the underlying pathophysiology of type 2 diabetes mellitus. In the present study, we aimed to identify and biochemically characterize proteins associated with diabetes mellitus in a Qatari population. METHODS:In a diabetes case-control study (175 cases, 16...

journal_title：Archives of biochemistry and biophysics

authors： Noordam R,van Heemst D,Suhre K,Krumsiek J,Mook-Kanamori DO

更新日期：2020-08-15 00:00:00

abstract：:This report describes the isolation, sequencing, and functional analysis of the sodA gene, encoding Mn-superoxide dismutase, from Streptococcus thermophilus AO54. The gene was found to encode a 201 amino acid polypeptide with 88 and 83% identity to SodA from Streptococcus mutans and Streptococcus agalacticae, respecti...

journal_title：Archives of biochemistry and biophysics

authors： Andrus JM,Bowen SW,Klaenhammer TR,Hassan HM

更新日期：2003-12-01 00:00:00

abstract：:The oxidation of quercetin by horseradish peroxidase/H(2)O(2) was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the absence of GSH revealed formation of at least 20 different products, a result in line with other studies reporting the ...

journal_title：Archives of biochemistry and biophysics

authors： Awad HM,Boersma MG,Vervoort J,Rietjens IM

更新日期：2000-06-15 00:00:00

abstract：:The hydrolysis of 32 X-phenyl-N-methanesulfonyl glycinates by papain was investigated. It was found that the variation in the Michaelis constants could be rationalized by the following correlation equation: log 1/Km = 0.61 pi '3 + 0.46 MR4 + 0.55 sigma + 2.00 with a correlation coefficient of 0.945. In this expression...

journal_title：Archives of biochemistry and biophysics

authors： Carotti A,Smith RN,Wong S,Hansch C,Blaney JM,Langridge R

更新日期：1984-02-15 00:00:00

abstract：:Nitric oxide (.NO) and peroxynitrite (ONOO-) inhibit enzymes that depend on metal cofactors or oxidizable amino acids for activity. Since xanthine oxidase (XO) is a 2(2Fe2S) enzyme having essential sulfhydryl groups linked with Mo-pterin cofactor function, the influence of .NO and ONOO- on purified bovine XO was deter...

journal_title：Archives of biochemistry and biophysics

authors： Houston M,Chumley P,Radi R,Rubbo H,Freeman BA

更新日期：1998-07-01 00:00:00

abstract：:Procedures for the purification of bovine muscle carbonic anhydrase (isoenzyme III) are described. The purified enzyme has a molecular weight near 29,000 and contains one Zn2+ ion per molecule. The sedimentation coefficient, s(0)20,w, is 2.8 X 10(-13) s, the isoelectric pH is 8.5, and A280(0.1%) = 2.07 cm-1. The CO2 h...

journal_title：Archives of biochemistry and biophysics

authors： Engberg P,Millqvist E,Pohl G,Lindskog S

更新日期：1985-09-01 00:00:00

abstract：:Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (SceADH) binds NAD+ and NADH less tightly and turns over substrates more rapidly than does horse (Equus caballus) liver alcohol dehydrogenase E isoenzyme (EcaADH), and neither enzyme uses NADP efficiently. Amino acid residues in the proposed adenylate binding po...

journal_title：Archives of biochemistry and biophysics

authors： Fan F,Plapp BV

更新日期：1999-07-15 00:00:00

abstract：:Thyroid peroxidase (TPO) simultaneously catalyzes two very different types of reaction in the thyroid gland- iodination and coupling. The present study addresses the mechanism of this simultaneous dual activity. Compound I, the two-electron oxidation product of TPO, exists in two different forms--an oxoferryl porphyri...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris ML,Doerge DR

更新日期：1996-06-01 00:00:00

abstract：:The effect of a protein matrix on the processing of glycoprotein glycans by Golgi enzymes from plant seedlings has been determined with an artificial glycoprotein system, comparing the processing rates of glycan-(biotinyl)Asn (or glycan-(biotinamidohexanoyl)Asn) substrates either free or bound to avidin. An analysis o...

journal_title：Archives of biochemistry and biophysics

authors： Lu Y,Wold F

更新日期：1991-04-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase from maize leaves was inactivated by pyridoxal 5'-phosphate in the dark and in the light. A two-step reversible mechanism is proposed for inactivation in the dark, which involves the formation of a noncovalent complex prior to a Schiff base with amino groups of the enzyme. Spectral anal...

journal_title：Archives of biochemistry and biophysics

authors： Podesta FE,Iglesias AA,Andreo CS

更新日期：1986-05-01 00:00:00

abstract：:Interactions of six carbohydrates (trehalose, sucrose, glucose, raffinose, inositol, and glycerol) with dry dipalmitoylphosphatidylcholine (DPPC) were studied using differential scanning calorimetry (DSC) and infrared spectroscopy (ir) in order to elucidate the mechanism by which some of these carbohydrates preserve s...

journal_title：Archives of biochemistry and biophysics

authors： Crowe LM,Crowe JH,Chapman D

更新日期：1985-01-01 00:00:00

abstract：:Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. Howeve...

journal_title：Archives of biochemistry and biophysics

authors： Liu YK,Lin TH,Liu PF

更新日期：2017-11-15 00:00:00

abstract：:To assess the effect of a polymeric surfactant, Pluronic P-105 on the activity of electron transport chains in the mitochondria of HL-60 cells, the bioreduction rates of two membrane-localized lipophilic spin probes, 16-doxylstearic acid methyl ester (16-DSME) and 5-doxylstearic acid (5-DS), were studied. In addition,...

journal_title：Archives of biochemistry and biophysics

authors： Rapoport N,Marin AP,Timoshin AA

更新日期：2000-12-01 00:00:00

abstract：:Reperfusion of ischemic myocardial tissue results in an increase in mitochondrial free radical production and declines in respiratory activity. The effects of ischemia and reperfusion on the activities of Krebs cycle enzymes, as well as enzymes involved in electron transport, were evaluated to provide insight into whe...

journal_title：Archives of biochemistry and biophysics

authors： Sadek HA,Humphries KM,Szweda PA,Szweda LI

更新日期：2002-10-15 00:00:00

abstract：:Antioxidative flavonoids, ubiquitously included in vegetables, fruits and teas, are expected to prevent degenerative diseases. It is unclear, however, whether flavonoids can enter the cellular nuclei and suppress the oxidative damage of DNA. Here, several flavonoids at the physiological concentration of 10 microM were...

journal_title：Archives of biochemistry and biophysics

authors： Kanazawa K,Uehara M,Yanagitani H,Hashimoto T

更新日期：2006-11-15 00:00:00

abstract：:This study systematically examined the characteristics of specific binding of adult diferric transferrin to its receptor using a Triton X-100 solubilized preparation from human placentas as the receptor source. The following information was obtained. The ionic strength for maximal binding is in the range of 0.1-0.3 M ...

journal_title：Archives of biochemistry and biophysics

authors： Tsunoo H,Sussman HH

更新日期：1983-08-01 00:00:00

abstract：:Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. To investigate the effect of the expression of DRG2 on cell growth, we constructed a human Jurkat-T-cell line that overexpresses DRG2. Overexp...

journal_title：Archives of biochemistry and biophysics

authors： Ko MS,Lee UH,Kim SI,Kim HJ,Park JJ,Cha SJ,Kim SB,Song H,Chung DK,Han IS,Kwack K,Park JW

更新日期：2004-02-15 00:00:00

abstract：:We have previously demonstrated that gangliosides were able to modulate the plasma membrane Ca2+-ATPase (PMCA) from porcine brain synaptosomes and porcine erythrocytes [Y. Zhao, X. Fan, F. Yang, X. Zhang, Arch. Biochem. Biophys. 427 (2004) 204-212 and J. Zhang, Y. Zhao, J. Duan, F. Yang, X. Zhang, Arch. Biochem. Bioph...

journal_title：Archives of biochemistry and biophysics

authors： Duan J,Zhang J,Zhao Y,Yang F,Zhang X

更新日期：2006-10-15 00:00:00

abstract：:The yeast Saccharomyces cerevisiae SOC8 DNA fragment was isolated as a wildtype dominant suppressor of the cdc8 mutation. The SOC8 has previously been proved to be allelic with URA6, encoding the UMP kinase in yeast cells. The protein has been purified to homogeneity. In this report, we describe the characteristics of...

journal_title：Archives of biochemistry and biophysics

authors： Jong A,Yeh Y,Ma JJ

更新日期：1993-07-01 00:00:00