Purification and some properties of carbonic anhydrase from bovine skeletal muscle.

abstract：:Glycophorin from human red blood cells was exposed to ozone in aqueous solution. Amino acid analysis of glycophorin exposed to a 10-fold molar excess of ozone showed that the only residue affected was methionine. Both methionine residues of the protein were oxidized to methionine sulfoxide. Exposure of the oxidized pr...

journal_title：Archives of biochemistry and biophysics

authors： Banerjee SK,Mudd JB

更新日期：1992-05-15 00:00:00

abstract：:The modes of action of a Vero toxin (VT2 or Shiga-like toxin II) from Escherichia coli, of ricin, and of alpha-sarcin were compared. Elongation factor 1 (EF1) and GTP-dependent Phe-tRNA binding to ribosomes in the presence of poly(U) was inhibited by these three toxins, but EF1 and guanylyl (beta, gamma-methylene)-dip...

journal_title：Archives of biochemistry and biophysics

authors： Furutani M,Kashiwagi K,Ito K,Endo Y,Igarashi K

更新日期：1992-02-14 00:00:00

abstract：BACKGROUND:Proteomics is expected to provide novel insights in the underlying pathophysiology of type 2 diabetes mellitus. In the present study, we aimed to identify and biochemically characterize proteins associated with diabetes mellitus in a Qatari population. METHODS:In a diabetes case-control study (175 cases, 16...

journal_title：Archives of biochemistry and biophysics

authors： Noordam R,van Heemst D,Suhre K,Krumsiek J,Mook-Kanamori DO

更新日期：2020-08-15 00:00:00

abstract：:Horseradish peroxidase A1 thermal stability was studied by steady-state fluorescence, circular dichroism and differential scanning calorimetry at pH values of 4, 7 and 10. Changes in the intrinsic protein probes, tryptophan fluorescence, secondary structure, and heme group environment are not coincident. The T(m) valu...

journal_title：Archives of biochemistry and biophysics

authors： Carvalho AS,Melo EP,Ferreira BS,Neves-Petersen MT,Petersen SB,Aires-Barros MR

更新日期：2003-07-15 00:00:00

abstract：:Recently we reported that oxidant tumor promoters can induce the oxidative modification of protein kinase C (PKC) resulting in either activation or inactivation of the kinase (R. Gopalakrishna and W. B. Anderson, Arch. Biochem. Biophys. 285, 382-387, 1991). Since retinoids previously have been shown to antagonize the ...

journal_title：Archives of biochemistry and biophysics

authors： Gundimeda U,Hara SK,Anderson WB,Gopalakrishna R

更新日期：1993-01-01 00:00:00

abstract：:The major proteins to which 3-methylcholanthrene specifically binds have been purified over 480-fold with a 45% yield compared to a rat liver 100,000g supernate. The procedure involved a batch ion-exchange technique together with hydrophobic gel filtration and chromatofocusing chromatography. The multiple, specific 3-...

journal_title：Archives of biochemistry and biophysics

authors： Tierney B,Munzer S,Bresnick E

更新日期：1983-09-01 00:00:00

abstract：:The human folate receptor (hFR) is a plasma membrane protein that is anchored to the membrane via a glycosylphosphatidylinositol (GPI) tail in some cell types. The KB hFR cDNA sequence predicts a hydrophobic, alpha-helical 31-residue carboxyl terminus that is thought to be the signal for cleavage and attachment of the...

journal_title：Archives of biochemistry and biophysics

authors： Chung KN,Roberts S,Kim CH,Kirassova M,Trepel J,Elwood PC

更新日期：1995-09-10 00:00:00

abstract：:The conformation of a constrained peptide mimicking the putative first intracellular domain (iLP1) of thromboxane A(2) receptor (TP) was determined by (1)H 2D NMR spectroscopy. Through completed assignments of TOCSY, DQF-COSY, and NOESY spectra, a NMR structure of the peptide showed a beta-turn in residues 56-59 and a...

journal_title：Archives of biochemistry and biophysics

authors： Geng L,Wu J,So SP,Huang G,Ruan KH

更新日期：2004-03-15 00:00:00

abstract：:Recombinant CYP4F8 and CYP4F12 metabolize prostaglandin H2 (PGH2) analogs by omega2- and omega3-hydroxylation and arachidonic acid (20:4n-6) by omega3-hydroxylation. CYP4F8 was found to catalyze epoxidation of docosahexaenoic acid (22:6n-3) and docosapentaenoic acid (22:5n-3) and omega3-hydroxylation of 22:5n-6. CYP4F...

journal_title：Archives of biochemistry and biophysics

authors： Stark K,Wongsud B,Burman R,Oliw EH

更新日期：2005-09-15 00:00:00

abstract：:Thyroid peroxidase (TPO) was purified from human thyroid tissue, obtained at surgery from patients with Graves' disease, by a procedure similar to one that we had previously used for the purification of porcine TPO. The membrane-bound enzyme was solubilized by treatment of the thyroid particulate fraction with trypsin...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris ML,Yokoyama N,Slaughter C

更新日期：1990-05-01 00:00:00

abstract：:In this investigation we report the presence of two forms of inositol (1,4,5)P3/(1,3,4,5)P4-polyphosphate 5-phosphatase activity (types I and II) which were observed in soluble extracts of skeletal muscle after fractionation by DEAE-Sephacel chromatography. Hydrolysis of D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P...

journal_title：Archives of biochemistry and biophysics

authors： Hansbro PM,Foster PS,Hogan SP,Ozaki S,Denborough MA

更新日期：1994-05-15 00:00:00

abstract：:Geranylgeranyl diphosphate synthase supplies the essential acyclic precursor for Taxol biosynthesis in methyl jasmonate-induced Taxus canadensis suspension cell cultures. A cDNA encoding this prenyltransferase was cloned from an induced T. canadensis cell library. The recombinant enzyme expressed in yeast was confirme...

journal_title：Archives of biochemistry and biophysics

authors： Hefner J,Ketchum RE,Croteau R

更新日期：1998-12-01 00:00:00

abstract：:The effects of extracellular ATP on phosphoinositide metabolism and intracellular Ca2+ homeostasis were studied in Ehrlich ascites tumor cells. Cytosolic [Ca2+] was measured using either quin 2 or the recently described indicator fura 2. Addition of 0.5-25 microM extracellular ATP to intact cells results in a rapid mo...

journal_title：Archives of biochemistry and biophysics

authors： Dubyak GR

更新日期：1986-02-15 00:00:00

abstract：:Secreted frizzled-related protein (sFRP)-1 is a Wnt antagonist that inhibits breast carcinoma cell motility, whereas the secreted glycoprotein thrombospondin-1 stimulates adhesion and motility of the same cells. We examined whether thrombospondin-1 and sFRP-1 interact directly or indirectly to modulate cell behavior. ...

journal_title：Archives of biochemistry and biophysics

authors： Martin-Manso G,Calzada MJ,Chuman Y,Sipes JM,Xavier CP,Wolf V,Kuznetsova SA,Rubin JS,Roberts DD

更新日期：2011-05-15 00:00:00

abstract：:The direct membrane effect of propranolol was studied in vitro on washed platelet preparations. Propranolol has been reported to inhibit platelet aggregation by mechanisms unrelated to its beta-blocking activity. In the present study, the drug was found to enhance 1-anilino-8-naphthalene sulfonate binding to platelet ...

journal_title：Archives of biochemistry and biophysics

authors： Dash D,Rao GR

更新日期：1990-02-01 00:00:00

abstract：:The dependence of respiratory flux via the alternative pathway on the redox poise of the ubiquinone (Q) pool was investigated in soybean cotyledon mitochondria. A marked nonlinear relationship was observed between Q-pool reduction level and O2 uptake via the alternative oxidase. Significant engagement of the alternati...

journal_title：Archives of biochemistry and biophysics

authors： Dry IB,Moore AL,Day DA,Wiskich JT

更新日期：1989-08-15 00:00:00

abstract：:A numerical method was applied to a system of differential rate equations describing the monomer-dimer-inhibitor (M-D-I) interaction involving human immunodeficiency virus type 1 protease and a peptidomimetic, competitive inhibitor. Two pairs of progress curves were obtained, one involving the M-D interaction and the ...

journal_title：Archives of biochemistry and biophysics

authors： Morelock MM,Graham ET,Erdman D,Pargellis CA

更新日期：1996-04-15 00:00:00

abstract：:The primary site of action for the novel antibiotic, bicyclomycin, in Escherichia coli has been identified to be the rho transcription termination factor. The inhibition of rho poly(C)-stimulated hydrolysis of ATP by bicyclomycin has been found to proceed by a non-competitive, reversible pathway with respect to ATP (K...

journal_title：Archives of biochemistry and biophysics

authors： Park HG,Zhang X,Moon HS,Zwiefka A,Cox K,Gaskell SJ,Widger WR,Kohn H

更新日期：1995-11-10 00:00:00

abstract：:The kinetics and thermodynamics of Ulex europeas agglutinin 1-H-antigenic determinant was studied by monitoring the binding of the lectin to H-antigenic fucolipid incorporated in liposomes tethered to the sensor surface by surface plasmon resonance. The second-order rate constants for the interactions are several orde...

journal_title：Archives of biochemistry and biophysics

authors： Thomas CJ,Surolia A

更新日期：2000-02-01 00:00:00

abstract：:Systemic inflammatory response syndrome (SIRS) is associated with excessive inflammatory response, however, the pathophysiology of inflammation is poorly understood. The retinoid-related orphan receptor α (RORα) is a key inflammatory regulator, but the mechanisms underlying its role remain unclear. The aim of this stu...

journal_title：Archives of biochemistry and biophysics

authors： Han S,Li Z,Han F,Jia Y,Qi L,Wu G,Cai W,Xu Y,Li C,Zhang W,Hu D

更新日期：2019-06-15 00:00:00

abstract：:The effects of fluorescein isothiocyanate II (FITC) on the actions of insulin in rat adipocytes were studied. When adipocytes were incubated with FITC at pH 7.4 (2 mM agent, 8 min), the cells were completely deprived of their specific insulin-binding activity and rendered unresponsive to the hormone. The effect of FIT...

journal_title：Archives of biochemistry and biophysics

authors： Goto Y,Sumida Y,Flanagan JE,Robinson FW,Simpson IA,Cushman SW,Kono T

更新日期：1992-03-01 00:00:00

abstract：:Linamarase (EC 3.2.1.21) was purified from cassava petiole, stem, and root cortex by ammonium sulfate precipitation, column chromatography on Sepharose 6B, and chromatofocusing. The last step resolved the enzyme from each source into three forms with pI values of 4.3, 3.3, and 2.9. Each form was found to be oligomeric...

journal_title：Archives of biochemistry and biophysics

authors： Eksittikul T,Chulavatnatol M

更新日期：1988-10-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase from maize leaves was inactivated by pyridoxal 5'-phosphate in the dark and in the light. A two-step reversible mechanism is proposed for inactivation in the dark, which involves the formation of a noncovalent complex prior to a Schiff base with amino groups of the enzyme. Spectral anal...

journal_title：Archives of biochemistry and biophysics

authors： Podesta FE,Iglesias AA,Andreo CS

更新日期：1986-05-01 00:00:00

abstract：:Glycosaminoglycan (GAG)-protein interactions regulate a myriad of physiologic and pathologic processes, yet an understanding of how these molecules interact is lacking. The role of the pattern and spacing of basic amino acids (arginine (R) and lysine (K)) in heparin binding sites was investigated using peptide analogs...

journal_title：Archives of biochemistry and biophysics

authors： Fromm JR,Hileman RE,Caldwell EE,Weiler JM,Linhardt RJ

更新日期：1997-07-01 00:00:00

abstract：:Adenylate kinase (Adk) that catalyses the synthesis of ADP from ATP and AMP has also been shown to perform an ATP dependent phosphorylation of ribo- and deoxynucleoside diphosphates to their corresponding nucleoside triphosphate; ATP+(d)NDP<-->ADP+(d)NTP. This reaction, suggested to occur by the transfer of the gamma-...

journal_title：Archives of biochemistry and biophysics

authors： Willemoës M,Kilstrup M

更新日期：2005-12-15 00:00:00

abstract：:Oxidation of [2,3-14C]succinate carbons in the mitochondrial Krebs cycle was used as a probe to investigate the effects of insulin, epinephrine, glucagon, and 2,4-dinitrophenol (2,4-DNP) on isolated rat hepatocytes. Epinephrine, glucagon, and 2,4-DNP had a far greater stimulatory effect on 14CO2 formation from [2,3-14...

journal_title：Archives of biochemistry and biophysics

authors： Mohan C,Memon RA,Bessman SP

更新日期：1991-05-15 00:00:00

abstract：:An improved high yielding procedure for the purification of myrosinase from Sinapis alba L. consisting of concanavalin A affinity chromatography followed by a chromatofocusing step is presented. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate-gel electrophoresis and by analytical ultracentrifug...

journal_title：Archives of biochemistry and biophysics

authors： Pessina A,Thomas RM,Palmieri S,Luisi PL

更新日期：1990-08-01 00:00:00

abstract：:For a set of 10 para-substituted toluene derivatives, three enzymatic constants were determined describing their interaction with purified rabbit liver microsomal P-450LM2. The three constants were the catalytic rate constant (Kcat) for hydroxylation, the apparent dissociation constant (Kd) for the enzyme-substrate co...

journal_title：Archives of biochemistry and biophysics

authors： White RE,McCarthy MB

更新日期：1986-04-01 00:00:00

abstract：:CYP119 from Sulfolobus solfataricus is the first well-characterized thermophilic cytochrome P450 enzyme. The endogenous substrate for this enzyme is not known but it hydroxylates lauric acid in a reaction supported by surrogate mesophilic electron donors. However, reconstitution of a high-temperature catalytic system ...

journal_title：Archives of biochemistry and biophysics

authors： Puchkaev AV,Ortiz de Montellano PR

更新日期：2005-02-01 00:00:00

abstract：:Cocoa flavonoids are able to reduce cardiovascular risk by improving endothelial function and decreasing blood pressure (BP). Interest in the biological activities of cocoa is daily increasing. A recent meta-analysis shows flavanol-rich cocoa administration decreases mean systolic (-4.5mm Hg; p<0.001) and diastolic (-...

journal_title：Archives of biochemistry and biophysics

authors： Grassi D,Desideri G,Ferri C

更新日期：2010-09-01 00:00:00