Regulation of glycan processing by Golgi enzymes from red kidney bean (Phaseolus vulgaris) seedlings.

abstract：:A quinone produced from veratryl alcohol by lignin peroxidase from the white rot fungus Phanerochaete chrysosporium was tested for its ability to mediate reduction. The quinone (2-hydroxymethyl-5-methoxy-1,4-benzoquinone), reduced chemically or by cellobiose:quinone reductase isolated from cultures of the fungus, medi...

journal_title：Archives of biochemistry and biophysics

authors： Rasmussen SJ,Chung N,Khindaria A,Grover TA,Aust SD

更新日期：1995-07-10 00:00:00

abstract：:The aim of the present study was to further understand how changes in membrane organization can lead to higher rates of lipid oxidation. We previously demonstrated that Al(3+), Sc(3+), Ga(3+), Be(2+), Y(3+), and La(3+) promote lipid packing and lateral phase separation. Using the probe Laurdan, we evaluated in liposom...

journal_title：Archives of biochemistry and biophysics

authors： Verstraeten SV,Oteiza PI

更新日期：2000-03-15 00:00:00

abstract：:The expression and induction of cytochrome P450 by 2,3,7,8-tetrachlordibenzo-p-dioxin (TCDD) and beta-naphthoflavone (BNF) in a new liver cell line from adult zebrafish (Brachydanio rerio) were studied. Subcellular fractions from control, BNF- or TCDD-treated cells did not show detectable bands in immunoblots probed w...

journal_title：Archives of biochemistry and biophysics

authors： Miranda CL,Collodi P,Zhao X,Barnes DW,Buhler DR

更新日期：1993-09-01 00:00:00

abstract：:Reactive oxygen species (ROS) can be generated in experimental shock states through several different mechanisms. We measured ROS production in metabolically active liver mitochondria from rats rendered septic by cecal ligation and puncture. By polarography, the State 4 and State 3 respiration rates of liver mitochond...

journal_title：Archives of biochemistry and biophysics

authors： Taylor DE,Ghio AJ,Piantadosi CA

更新日期：1995-01-10 00:00:00

abstract：:M. Kuwajima, C. B. Newgard, D. W. Foster, and J. D. McGarry (1986, J. Biol. Chem. 261, 8849-8853) have concluded that the reason postprandial hepatic glycogenesis occurs primarily from gluconeogenic precursors rather than glucose is because glucokinase activity is insufficient to support the observed rates of glycogen...

journal_title：Archives of biochemistry and biophysics

authors： Davidson AL,Arion WJ

更新日期：1987-02-15 00:00:00

abstract：:Primary carnitine deficiency is an autosomal recessive disorder caused by defective carnitine transport which impairs fatty acid oxidation and manifests as nonketotic hypoglycemia or skeletal or heart myopathy. Here we report the functional characterization of this transporter in human fibroblasts. Carnitine enters no...

journal_title：Archives of biochemistry and biophysics

authors： Scaglia F,Wang Y,Longo N

更新日期：1999-04-01 00:00:00

abstract：:Studies were carried out at pH 7.0 and gamma/2 0.15 before addition of CaCl2 or EDTA. Clotting time, tau, at 3.03 microM fibrinogen and 0.91 u/ml thrombin was determined for equilibrium systems. With added Ca2+, tau decreases, from tau 0 at 0 added Ca2+ (mean, 29.7 +/- 3 s), by approximately 3 s at 5 mM added Ca2+. Wi...

journal_title：Archives of biochemistry and biophysics

authors： Perizzolo KE,Sullivan S,Waugh DF

更新日期：1985-03-01 00:00:00

abstract：:To establish a cell line expressing enhanced levels of beta-amyloid precursor protein (beta-APP), we constructed plasmid DNAs expressing beta-APP-751 mRNA and transfected them into COS-1 cells. Using a modified version of the reverse transcriptase polymerase chain reaction which is RNA sensitive to study the beta-APP ...

journal_title：Archives of biochemistry and biophysics

authors： Ramakrishna N,Smedman M,Gillam B

更新日期：1996-02-15 00:00:00

abstract：:We recently cloned a rat brain agmatinase-like protein (ALP) whose amino acid sequence greatly differs from other agmatinases and exhibits a LIM-like domain close to its carboxyl terminus. The protein was immunohistochemically detected in the hypothalamic region and hippocampal astrocytes and neurons. We now show that...

journal_title：Archives of biochemistry and biophysics

authors： Castro V,Fuentealba P,Henríquez A,Vallejos A,Benítez J,Lobos M,Díaz B,Carvajal N,Uribe E

更新日期：2011-08-01 00:00:00

abstract：:We hypothesized that cellular oxygen consumption is abnormal during sepsis as a result of increased oxidative stress and selective mitochondrial damage. In a rat model of sepsis (cecal ligation and puncture), we studied the respiratory characteristics of isolated hepatocytes and liver mitochondria 16 h after onset of ...

journal_title：Archives of biochemistry and biophysics

authors： Kantrow SP,Taylor DE,Carraway MS,Piantadosi CA

更新日期：1997-09-15 00:00:00

abstract：:Conversion of a steroid receptor complex from the 8 S to the 4S form results in new interactions between the steroid and the receptor and/or formation of new intra-protein bonds within the receptor molecule itself. These bonds must be broken before the steroid is released. In order to localize these newly formed inter...

journal_title：Archives of biochemistry and biophysics

authors： Wolfson AJ,Ryu ML,Baghdady R

更新日期：1991-02-01 00:00:00

abstract：:Two enzymatically modified derivatives of tobramycin have been prepared by gentamicin nucleotidyl transferase-catalyzed adenylylation of tobramycin, using ATP and (Sp)-ATP alpha S as adenylylation substrates. (EC 2.7.7.46). The 1H, 13C, and 31P NMR spectra have been assigned for tobramycin, 2''-(adenosine-5'-phosphory...

journal_title：Archives of biochemistry and biophysics

authors： Van Pelt JE,Mooberry ES,Frey PA

更新日期：1990-08-01 00:00:00

abstract：:The chondrocytes of adult articular cartilage rely upon transport phenomena within their avascular extracellular matrix for many biological activities. Therefore, changes in matrix structure which influence cytokine transport parameters may be an important mechanism involved in the chondrocyte response to tissue compr...

journal_title：Archives of biochemistry and biophysics

authors： Quinn TM,Kocian P,Meister JJ

更新日期：2000-12-15 00:00:00

abstract：:Human granzyme 3 (Gr3) is a serine protease contained in the granules of natural killer cells and cytotoxic T lymphocytes. To elucidate the biochemical and physiological characteristics of Gr3, we attempted to prepare an enzymatically active recombinant human Gr3 without refolding and proteolytic activation. An expres...

journal_title：Archives of biochemistry and biophysics

authors： Hirata Y,Inagaki H,Shimizu T,Li Q,Nagahara N,Minami M,Kawada T

更新日期：2006-02-01 00:00:00

abstract：:The rates of reactive absorption of ozone by various biomolecule solutions were measured. At pH 7, the ability of various biomolecules to reactively absorb ozone was in the following sequence: thiosulfate > ascorbate > cysteine approximately methionine > glutathione. The rates of reactive absorption under a variety of...

journal_title：Archives of biochemistry and biophysics

authors： Kanofsky JR,Sima PD

更新日期：1995-01-10 00:00:00

abstract：:Tetrahydro-dUMP, an analog of the putative transition state in aminohydrolysis of deoxycytidine monophosphate (dCMP) inhibits the allosteric enzyme deoxycytidylate aminohydrolase with high affinity. The inhibition is reversible, and its kinetics is consistent with the analog binding at the substrate site only to one a...

journal_title：Archives of biochemistry and biophysics

authors： Nucci R,Febbraio F,Piccialli G,de Napoli L,Vaccaro C,Rossi M,Whitehead EP

更新日期：1994-04-01 00:00:00

abstract：:The essential Zn(II) in bovine liver dihydropyrimidine amidohydrolase (DHPase) was removed by incubation with 2,6-dipicolinic acid and replaced with Mn(II). Electron paramagnetic resonance studies of Mn(II) binding show that there are four binding sites per tetramer, and the dissociation constant at pH 7.5 is 13.5 mic...

journal_title：Archives of biochemistry and biophysics

authors： Lee MH,Pettigrew DW,Sander EG,Nowak T

更新日期：1987-12-01 00:00:00

abstract：:The properties of porcine platelet acyltransferases which catalyze the incorporation of unsaturated fatty acids into the 2 positions of phospholipids were compared with those of porcine liver microsomes and rat liver microsomes. There were significant differences in the relative rates of incorporation of acyl groups i...

journal_title：Archives of biochemistry and biophysics

authors： Inoue M,Murase S,Okuyama H

更新日期：1984-05-15 00:00:00

abstract：:We have isolated an activator of collagenase from medium conditioned with articular cartilage. The activity is contained in an acidic protein appearing as a doublet band of Mr 57,000 and 56,000 on sodium dodecyl sulfate polyacrylamide gels. Both components of the doublet have identical isoelectric points as demonstrat...

journal_title：Archives of biochemistry and biophysics

authors： Treadwell BV,Neidel J,Pavia M,Towle CA,Trice ME,Mankin HJ

更新日期：1986-12-01 00:00:00

abstract：:Hippocampal-dependent cognitive functions rely on production of new neurons and maintenance of dendritic structures to provide the synaptic plasticity needed for learning and formation of new memories. Hippocampal formation is exquisitely sensitive to patho-physiological changes, and reduced antioxidant capacity and e...

journal_title：Archives of biochemistry and biophysics

authors： Huang TT,Leu D,Zou Y

更新日期：2015-06-15 00:00:00

abstract：:This study characterizes the structural and functional significance of sulfhydryl residues in human plasma heparin cofactor II (HCII). For quantification of sulfhydryl groups, the extinction coefficient of HCII was redetermined and found to be 0.593 ml mg-1 cm-1 using second-derivative spectroscopy and multicomponent ...

journal_title：Archives of biochemistry and biophysics

authors： Church FC,Meade JB,Pratt CW

更新日期：1987-12-01 00:00:00

abstract：:In hepatocytes incubated in the presence of 20 mM glucose, strong stimulation of glycogen synthesis by LiCl was observed in cells from both normal and streptozotocin-induced diabetic rats. This stimulation was concentration- and time-dependent, and it was related to an increase in the glycogen synthase activity ratio....

journal_title：Archives of biochemistry and biophysics

authors： Rodriguez-Gil JE,Guinovart JJ,Bosch F

更新日期：1993-03-01 00:00:00

abstract：:Incubation of rat hepatocytes with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), an activator of the 5'-AMP-activated protein kinase (AMPK), produced a twofold stimulation of palmitate oxidation and of the activity of carnitine palmitoyltransferase I (CPT-I), together with a profound decrease of the activity ...

journal_title：Archives of biochemistry and biophysics

authors： Velasco G,Geelen MJ,Guzmán M

更新日期：1997-01-15 00:00:00

abstract：:Recombinant soluble murine interleukin 5 receptor alpha (sIL-5R alpha) was expressed in Chinese hamster ovary cells and its biological activity was characterized. By using murine IL-5-coupled Sepharose, the single step purification method of the protein was established. The purified protein inhibited the murine IL-5-i...

journal_title：Archives of biochemistry and biophysics

authors： Tsuruoka N,Yamashiro K,Tsujimoto M

更新日期：1993-11-15 00:00:00

abstract：:Seven carboxylesterase isozymes were purified to electrophoretic homogeneity from liver microsomes of mouse, hamster, guinea pig, rabbit, and monkey by the same procedure used previously to obtain three isozymes from the rat, and their physical, enzymological, and immunological properties were compared with those of t...

journal_title：Archives of biochemistry and biophysics

authors： Hosokawa M,Maki T,Satoh T

更新日期：1990-03-01 00:00:00

abstract：:The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

journal_title：Archives of biochemistry and biophysics

authors： Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

更新日期：1999-03-15 00:00:00

abstract：:Antioxidant defenses include a group of ubiquitous, non-heme peroxidases, designated the peroxiredoxins, which rely on an activated cysteine residue at their active site to catalyze the reduction of hydrogen peroxide, organic hydroperoxides, and peroxynitrite. In the typical 2-Cys peroxiredoxins, a second cysteinyl re...

journal_title：Archives of biochemistry and biophysics

authors： Poole LB

更新日期：2005-01-01 00:00:00

abstract：:Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

journal_title：Archives of biochemistry and biophysics

authors： Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

更新日期：1984-06-01 00:00:00

abstract：:Carbohydrate Binding Modules (CBMs) targeting cellulose, xylan and mannan have been reported, however, a CBM targeting rhamnogalacturonan I (RG I) has never been identified. We had studied earlier a rhamnogalacturonan lyase (CtRGL) from Clostridium thermocellum that was associated with a family 35 CBM, Rgl-CBM35. In t...

journal_title：Archives of biochemistry and biophysics

authors： Dhillon A,Sharma K,Rajulapati V,Goyal A

更新日期：2018-09-15 00:00:00

abstract：:Protein tyrosine kinase Yes is a cellular homolog of v-Yes, the oncogenic protein product of avian sarcoma virus Y73. Yes is a member of the Src family and its activation has been associated with several types of human cancer. Human Yes has not been previously characterized enzymatically. To carry out biochemical char...

journal_title：Archives of biochemistry and biophysics

authors： Sun G,Budde RJ

更新日期：1997-09-01 00:00:00