Purification and characterization of collagenase activator protein synthesized by articular cartilage.

abstract：:Diabetic surgical patients are at risk for peri- and post-operative complications, which can be prevented by maintaining tight glycemic control during anesthesia. Control of blood sugar would decrease unwanted chemical reactions, such as protein glycation, minimizing tissue dysfunction. Methylglyoxal (MG) is a major c...

journal_title：Archives of biochemistry and biophysics

authors： Pattin AE,Ochs S,Theisen CS,Fibuch EE,Seidler NW

更新日期：2010-06-01 00:00:00

abstract：:(+)-Menthofuran is an undesirable monoterpenoid component of peppermint (Mentha x piperita) essential oil that is derived from the alpha,beta-unsaturated ketone (+)-pulegone. Microsomal preparations, from the oil gland secretory cells of a high (+)-menthofuran-producing chemotype of Mentha pulegium, transform (+)-pule...

journal_title：Archives of biochemistry and biophysics

authors： Bertea CM,Schalk M,Karp F,Maffei M,Croteau R

更新日期：2001-06-15 00:00:00

abstract：:Binding of ligands to the catalytic center of mammalian triosephosphate isomerase (TPI) induces a conformational change(s) that enhances the specific deamidation of Asn71 at the subunit interface. Deamidation initiates dissociation and degradation of the enzyme in vivo and in vitro. We have utilized circular dichroism...

journal_title：Archives of biochemistry and biophysics

authors： Sun AQ,Yüksel KU,Rao GS,Gracy RW

更新日期：1992-06-01 00:00:00

abstract：:L-Tryptophan is an essential amino acid for mammals. It is utilized not only for protein synthesis but also for the biosynthesis of serotonin and melatonin by the serotonin pathway as well as nicotinamide adenine dinucleotide by the kynurenine pathway. Although the kynurenine pathway is responsible for the catabolism ...

journal_title：Archives of biochemistry and biophysics

authors： Geng J,Liu A

更新日期：2014-02-15 00:00:00

abstract：:Glyoxalase I, a member of the metalloglutathione (GSH) transferase superfamily, plays a critical detoxification role in cells by catalyzing the conversion of cytotoxic methylglyoxal (as the diastereomeric GSH-thiohemiacetals) to S-D-lactoylglutathione via a 1,2-hydrogen transfer. The mechanism-of-action of this Zn2+-m...

journal_title：Archives of biochemistry and biophysics

authors： Creighton DJ,Hamilton DS

更新日期：2001-03-01 00:00:00

abstract：:Interactions between loops 2, 2' and 4, known as the loop bundle, stabilize the active site of caspase-3. Loop 4 (L4) is of particular interest due to its location between the active site and the dimer interface. We have disrupted a salt bridge between K242 and E246 at the base of L4 to determine its role in overall c...

journal_title：Archives of biochemistry and biophysics

authors： Walters J,Swartz P,Mattos C,Clark AC

更新日期：2011-04-01 00:00:00

abstract：:We reported previously a highly purified phosphatidylinositol-specific phospholipase C (PI-PLC) from bovine brain and from human myelin which was stimulated by myelin basic protein. In this paper we report that the stimulation of the PI-PLC activity by myelin basic protein (MBP) requires arginine residues in peptide l...

journal_title：Archives of biochemistry and biophysics

authors： Tompkins TA,Moscarello MA

更新日期：1993-05-01 00:00:00

abstract：:Autologous venous grafts, used to circumvent occluded coronary arteries during coronary artery bypass, often develop thrombosis and neointimal hyperplasia. During neointimal hyperplasia, vascular smooth muscle cells (VSMCs), exposed to substantially higher pressure and hemodynamic forces, proliferate and extracellular...

journal_title：Archives of biochemistry and biophysics

authors： Song L,Duan P,Guo P,Li D,Li S,Xu Y,Zhou Q

更新日期：2012-12-15 00:00:00

abstract：:The motility of actin filaments interacting with heavy meromyosin molecules was directly observed on indium tin oxide-coated glass (ITO-glass), over which a surface current flowed. Because the increase in surface current applied to ITO-glass increases the temperature, we focused on the temperature-dependence of the sl...

journal_title：Archives of biochemistry and biophysics

authors： Wada R,Sato D,Nakamura T,Hatori K

更新日期：2015-11-15 00:00:00

abstract：:The development and improvement of fluorescent Zn2+ sensors and Zn2+ imaging techniques have increased our insight into this biologically important ion. Application of these tools has identified an intracellular labile Zn2+ pool and cultivated further interest in defining the distribution and dynamics of labile Zn2+. ...

journal_title：Archives of biochemistry and biophysics

authors： Carpenter MC,Lo MN,Palmer AE

更新日期：2016-12-01 00:00:00

abstract：:Forskolin, a diterpene that exerts several pharmacological effects, activates adenylate cyclase in brain and in some other mammalian tissues. Properties of forskolin activation of adenylate cyclase from central nervous system of the dipterous Ceratitis capitata are described. The interaction of forskolin with the inse...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Municio AM

更新日期：1987-04-01 00:00:00

abstract：:A large number of mutations affecting the F(0) sector of Escherichia coli F(1)F(0) ATP synthase have been constructed and characterized. A subset of the missense mutations resulted in fully assembled enzyme complexes blocked in proton translocation and displaying marked decreases in ATP hydrolysis activity. The cataly...

journal_title：Archives of biochemistry and biophysics

authors： Gardner JL,Cain BD

更新日期：2000-08-01 00:00:00

abstract：:The oxidative DNA damage by the cupric complexes of sisomicin was investigated in the presence of varying amounts of histidine, ATP, Mg(II) ions or phosphates. We found that by very low concentrations, the amino acid is able to inhibit the cleavage totally. This occurs both by its competition with antibiotic for coppe...

journal_title：Archives of biochemistry and biophysics

authors： Szczepanik W,Swiatek M,Skała J,Jezowska-Bojczuk M

更新日期：2004-11-01 00:00:00

abstract：:The digestion of tubulin with subtilisin and the reassociation of the digestion products was followed by means of the fluorescent probe 4',6-diamidino-2-phenylindole (DAPI). The fluorescence spectra of DAPI bound to chicken brain tubulin and to the main products of tubulin digested with subtilisin-agarose (tubulin S a...

journal_title：Archives of biochemistry and biophysics

authors： Ortiz M,Lagos R,Monasterio O

更新日期：1993-05-15 00:00:00

abstract：:Information about the molecular mechanisms leading to the activation of the osteoclast is relatively limited. While there is compelling evidence that the signaling mechanisms of Src and integrin β3 are essential for osteoclast activation, the regulation of these two signaling mechanisms is not fully understood. In thi...

journal_title：Archives of biochemistry and biophysics

authors： Lau KW,Sheng MH

更新日期：2018-07-15 00:00:00

abstract：:Coupling of the narrow 311-nm Gd3+ fluorescence transition with the vibrational transitions of its inner-sphere ligands gives rise to the emission of vibronic photons between 320 and 350 nm. The energy difference between the fluorescence and vibronic transitions is equal to that of the vibrational transitions of the m...

journal_title：Archives of biochemistry and biophysics

authors： MacGregor RB Jr

更新日期：1989-10-01 00:00:00

abstract：:In trout and mammals, the major extrahepatic expression site for CYP3A forms is in the intestine. A cDNA encoding a new CYP3A subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (RT-PCR), followed by rapid amplification of cDNA ends (RACE)-PCR. In a set o...

journal_title：Archives of biochemistry and biophysics

authors： Lee SJ,Buhler DR

更新日期：2003-04-01 00:00:00

abstract：:Here we investigated whether cADPR and NAADP are synthesized in mitochondria. We found that ADPR-cyclase activity is present in mitochondria. In addition, we describe for the first time synthesis of NAADP in this intracellular organelle. ADPR-cyclase activities (V(MAX)) and NAADP synthesis in mitochondria were about 4...

journal_title：Archives of biochemistry and biophysics

authors： Liang M,Chini EN,Cheng J,Dousa TP

更新日期：1999-11-15 00:00:00

abstract：:Accumulating evidence suggests that oxidative stress resulting in reactive oxygen species generation plays a pivotal role in neurodegenerative diseases, supporting the realization of the use of radical scavengers, metal chelator agents, such as the natural polyphenols for therapy. In this study, we have focused on spe...

journal_title：Archives of biochemistry and biophysics

authors： Weinreb O,Amit T,Youdim MB

更新日期：2008-08-15 00:00:00

abstract：:The common notion in the protein world holds that proteins are synthesized as a linear polypeptide chain, followed by folding into a unique, functional 3D-structure. As outlined in many articles of this volume, this is in fact the case for a great proportion of the proteome. Many proteins and protein domains, however,...

journal_title：Archives of biochemistry and biophysics

authors： Kovacs D,Szabo B,Pancsa R,Tompa P

更新日期：2013-03-01 00:00:00

abstract：:The ability of chelators and ascorbic acid to mobilize iron from crocidolite, amosite, medium- and short-fiber chrysotile, and tremolite was investigated. Ferrozine, a strong Fe(II) chelator, mobilized Fe(II) from crocidolite (6.6 nmol/mg asbestos/h) and amosite (0.4 nmol/mg/h) in 50 mM NaCl, pH 7.5. Inclusion of asco...

journal_title：Archives of biochemistry and biophysics

authors： Lund LG,Aust AE

更新日期：1990-04-01 00:00:00

abstract：:The sigma(N) protein is an alternative sigma subunit of bacterial RNA polymerase. We investigated the role of a 12-amino-acid "tail" at the C-terminus of Klebsiella pneumoniae sigma(N), which was predicted to be largely surface-exposed and to be mostly loop (that is not alpha-helical or beta-strand). Deletion of this ...

journal_title：Archives of biochemistry and biophysics

authors： Studholme DJ,Finn RD,Chaney MK,Buck M

更新日期：1999-11-15 00:00:00

abstract：:Tobacco-induced oxidative stress leads to chronic inflammation and is implicated in the development of many human epithelial cancers, including head and neck cancer. Cigarette smoke exposure was shown to induce the expression of the ΔNp63α and nitric oxide synthase (NOS)-2 in head and neck squamous cell carcinoma cell...

journal_title：Archives of biochemistry and biophysics

authors： Ratovitski EA

更新日期：2011-02-15 00:00:00

abstract：:Resonance Raman spectroscopy (RRS) is a non-invasive method that has been developed to assess carotenoid status in human tissues including human skin in vivo. Skin carotenoid status has been suggested as a promising biomarker for human studies. This manuscript describes research done relevant to the development of thi...

journal_title：Archives of biochemistry and biophysics

authors： Mayne ST,Cartmel B,Scarmo S,Jahns L,Ermakov IV,Gellermann W

更新日期：2013-11-15 00:00:00

abstract：:The parent pyridine nucleotide NAD is the end product of oxidative tryptophan catabolism via the kynurenine pathway. Indoleamine 2, 3-dioxygenase, the rate-limiting enzyme for this pathway, is induced by the proinflammatory cytokine interferon-gamma. The aim of this study was to investigate the effect of interferon-ga...

journal_title：Archives of biochemistry and biophysics

authors： Grant RS,Passey R,Matanovic G,Smythe G,Kapoor V

更新日期：1999-12-01 00:00:00

abstract：:The total lipid composition of highly purified plasma membranes from light-grown barley (Hordeum vulgare) leaves was investigated. The plasma membranes were separated from intracellular membranes by subfractionation of the microsomal fraction using aqueous polymer two-phase partitioning. A novel finding was that gluco...

journal_title：Archives of biochemistry and biophysics

authors： Rochester CP,Kjellbom P,Andersson B,Larsson C

更新日期：1987-06-01 00:00:00

abstract：:The oxidized cytochrome c(2) from the purple phototrophic bacteria, Rhodobacter sphaeroides and Rhodobacter capsulatus, bind the neutral species of imidazole (K(a) = 1440 +/- 40 M(-1)) 50 times more strongly than does horse mitochondrial cytochrome c (K(a) = 30 +/- 1 M(-1)). The kinetics of imidazole binding are consi...

journal_title：Archives of biochemistry and biophysics

authors： Dumortier C,Meyer TE,Cusanovich MA

更新日期：1999-11-15 00:00:00

abstract：:Metallothionein isoform II was purified from calf liver. The protein had a metal content of 1.2-1.9 Cu ions and 5.6-6.2 Zn ions per molecule in different preparations. The complete amino acid sequence of the molecule was determined by automatic Edman degradation of CNBr and tryptic peptides of the carboxymethylated pr...

journal_title：Archives of biochemistry and biophysics

authors： Winge DR,Gray WR,Zelazowski A,Garvey JS

更新日期：1986-02-15 00:00:00

abstract：:The multifunctional tyrosine N-hydroxylase, cytochrome P450TYR (CYP79), from Sorghum bicolor catalyzing the conversion of tyrosine to p-hydroxyphenyl-acetaldoxime in the biosynthesis of the cyanogenic glucoside dhurrin, has been expressed in Escherichia coli using the isopropyl-beta-D-thiogalactopyranoside-inducible v...

journal_title：Archives of biochemistry and biophysics

authors： Halkier BA,Nielsen HL,Koch B,Møller BL

更新日期：1995-10-01 00:00:00

abstract：:The polyglutamation kinetics of methotrexate (MTX) in MCF-7 human breast cancer cells have been formulated mathematically. The model takes account of glutamation and hydrolysis kinetics up through the pentaglutamate level, increased synthesis of dihydrofolate reductase following exposure to drug, reversible tight-bind...

journal_title：Archives of biochemistry and biophysics

authors： Morrison PF,Allegra CJ

更新日期：1987-05-01 00:00:00