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Temperature control of the motility of actin filaments interacting with myosin molecules using an electrically conductive glass in the presence of direct current.

Abstract：

:The motility of actin filaments interacting with heavy meromyosin molecules was directly observed on indium tin oxide-coated glass (ITO-glass), over which a surface current flowed. Because the increase in surface current applied to ITO-glass increases the temperature, we focused on the temperature-dependence of the sliding velocity and the effect of the current flow on the orientation of filament motion. Using high precision fluorescence measurements, the displacement vectors of filaments were collected at intervals of 1/30 s. The direction of filament motion was independent to that of current flow up to 0.17 A (7.7 A/m of surface current density); however, the velocity increased by approximately 2-fold when the surface temperature increased from 25 °C to 37 °C. The moving actin filaments exhibited a broader velocity distribution at high temperature than at low temperature. Collectively, these data suggest that using ITO-glass with a surface current to generate a well-controlled temperature change may serve to evaluate temperature-dependent transient responses in protein activity under a microscope, without interference from electrical effects.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Wada R,Sato D,Nakamura T,Hatori K

doi

10.1016/j.abb.2015.10.001

subject

Has Abstract

pub_date

2015-11-15 00:00:00

pages

51-6

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(15)30076-X

journal_volume

586

pub_type

杂志文章

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