Techniques for measuring cellular zinc.

Abstract:

:The development and improvement of fluorescent Zn2+ sensors and Zn2+ imaging techniques have increased our insight into this biologically important ion. Application of these tools has identified an intracellular labile Zn2+ pool and cultivated further interest in defining the distribution and dynamics of labile Zn2+. The study of Zn2+ in live cells in real time using sensors is a powerful way to answer complex biological questions. In this review, we highlight newly engineered Zn2+ sensors, methods to test whether the sensors are accessing labile Zn2+, and recent studies that point to the challenges of using such sensors. Elemental mapping techniques can complement and strengthen data collected with sensors. Both mass spectrometry-based and X-ray fluorescence-based techniques yield highly specific, sensitive, and spatially resolved snapshots of metal distribution in cells. The study of Zn2+ has already led to new insight into all phases of life from fertilization of the egg to life-threatening cancers. In order to continue building new knowledge about Zn2+ biology it remains important to critically assess the available toolset for this endeavor.

journal_name

Arch Biochem Biophys

authors

Carpenter MC,Lo MN,Palmer AE

doi

10.1016/j.abb.2016.08.018

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

20-29

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(16)30297-1

journal_volume

611

pub_type

杂志文章,评审
  • Tumor necrosis factor-α-induced apoptosis of gastric cancer MKN28 cells: accelerated degradation of the inhibitor of apoptosis family members.

    abstract::The role of the inhibitor of apoptosis (IAP) family members in tumor necrosis factor-α (TNF-α)-induced apoptosis of human gastric cancer MKN28 cells was explored. TNF-α induced up-regulation of cIAP2, whereas cycloheximide (CHX) induced down-regulation of XIAP and survivin. Degradation of cIAP1 and XIAP, but not survi...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2014.12.003

    authors: Kitagawa M,Shiozaki A,Ichikawa D,Nakashima S,Kosuga T,Konishi H,Komatsu S,Fujiwara H,Okamoto K,Otsuji E

    更新日期:2015-01-15 00:00:00

  • Binding studies on aluminum(III)-albumin interaction.

    abstract::The present paper reports a quantitative investigation on the binding of aluminum to human serum albumin. Equilibrium dialysis and a general thermodynamic approach have been used to determine the binding parameters. Two aluminum binding sites have been identified on the albumin molecule, involving sites with single an...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/s0003-9861(03)00320-5

    authors: Zatta P,Dalla Via L,Di Noto V

    更新日期:2003-09-01 00:00:00

  • Recombinant human erythropoietin: purification and analysis of carbohydrate linkage.

    abstract::Erythropoietin was purified to homogeneity from the culture medium of a baby hamster kidney cell line stably transfected with a human erythropoietin gene. A three-step procedure was used, which included affinity chromatography, ion-exchange chromatography, and reverse-phase chromatography. Purity of the protein was co...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(88)90135-x

    authors: Broudy VC,Tait JF,Powell JS

    更新日期:1988-09-01 00:00:00

  • Biosynthesis and fate of proteoglycans in cartilage and bone during development and mineralization.

    abstract::Subcutaneous implantation of demineralized bone matrix in rats induces migration of host cells into the site and results in the sequential development of cartilage and bone. The biosynthesis and metabolic fate of proteoglycans in the plaques at the bone matrix implantation site were investigated by [35S]sulfate labeli...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(86)90551-5

    authors: Tian MY,Yanagishita M,Hascall VC,Reddi AH

    更新日期:1986-05-15 00:00:00

  • Fusion of sperm with prostasomes: effects on membrane fluidity.

    abstract::Prostasomes are membranous vesicles (150-200 nm diameter) present in human semen. They are secreted by the prostate gland and contain large amounts of cholesterol, sphingomyelin, and Ca2+. In addition, some of their proteins are enzymes. Prostasomes enhance the motility of ejaculated sperm and are involved in a number...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1997.9999

    authors: Carlini E,Palmerini CA,Cosmi EV,Arienti G

    更新日期:1997-07-01 00:00:00

  • A complex effect of arsenite on the formation of alpha-ketoglutarate in rat liver mitochondria.

    abstract::This investigation presents disturbances of the mitochondrial metabolism by arsenite, a hydrophilic dithiol reagent known as an inhibitor of mitochondrial alpha-keto acid dehydrogenases. Arsenite at concentrations of 0.1-1.0 mM was shown to induce a considerable oxidation of intramitochondrial NADPH, NADH, and glutath...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(90)90659-m

    authors: Lenartowicz E

    更新日期:1990-12-01 00:00:00

  • Calcium desensitizer catechin reverses diastolic dysfunction in mice with restrictive cardiomyopathy.

    abstract::Diastolic dysfunction refers to an impaired relaxation and an abnormality in ventricular blood filling during diastole while systolic function is preserved. Cardiac myofibril hypersensitivity to Ca(2+) is a major factor that causes impaired relaxation of myocardial cells. The present study investigates the effect of t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2015.03.015

    authors: Zhang L,Nan C,Chen Y,Tian J,Jean-Charles PY,Getfield C,Wang X,Huang X

    更新日期:2015-05-01 00:00:00

  • Direct proof that phorbol ester accelerates the use of choline phosphate for phosphatidylcholine synthesis in intact cells.

    abstract::Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, stimulates both the cellular uptake of radiolabeled choline and the activity of CTP:phosphocholine cytidylyltransferase resulting in increased incorporation of choline into phosphatidylcholine (PtdCho). Because of these multiple actions, it...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1996.0497

    authors: Kiss Z

    更新日期:1996-11-01 00:00:00

  • Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8.

    abstract::Actinomycin D (act-D) anchors itself into DNA-base pairs by intercalation and thereby inhibits mRNA synthesis. It has been well established that act-D elicits apoptosis in various cell types involving endothelial cells. However, the regulatory mechanisms of actinomycin D-induced apoptotic cell death still remain uncle...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2004.03.028

    authors: Shim D,Kang HY,Jeon BW,Kang SS,Chang SI,Kim HY

    更新日期:2004-05-15 00:00:00

  • Phospholipase A(2) is involved in thapsigargin-induced sodium influx in human lymphocytes.

    abstract::Previously, we reported that emptying of intracellular Ca(2+) pools with endoplasmatic Ca(2+)-ATP-ase inhibitor thapsigargin leads to the Na(+) influx in human lymphocytes (M. Tepel et al., 1994, J. Biol. Chem. 269, 26239-26242). In the present study we examined the mechanism underlying the thapsigargin-induced Na(+) ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1999.1588

    authors: Nofer JR,Junker R,Walter M,Seedorf U,Assmann G,Zidek W,Tepel M

    更新日期:2000-02-15 00:00:00

  • Cleavage of proteoglycan aggregate by leucocyte elastase.

    abstract::The partial degradation of proteoglycan aggregate by human leucocyte elastase yielded products that banded with Mr 190,000, 140,000, 88,000, and 71,000 when analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis. Analysis of these bands revealed that the 190,000- and 140,000-Da bands contained chondro...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(92)90014-n

    authors: Mok MT,Ilic MZ,Handley CJ,Robinson HC

    更新日期:1992-02-01 00:00:00

  • 1,25-Dihydroxyvitamin D₃ suppresses inflammation-induced expression of plasminogen activator inhibitor-1 by blocking nuclear factor-κB activation.

    abstract::Plasminogen activator inhibitor (PAI)-1 is a major fibrinolytic inhibitor. High PAI-1 is associated with increased renal and cardiovascular disease risk. Previous studies demonstrated PAI-1 down-regulation by 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃), but the molecular mechanism remains unknown. Here we show that exposur...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2010.12.020

    authors: Chen Y,Kong J,Sun T,Li G,Szeto FL,Liu W,Deb DK,Wang Y,Zhao Q,Thadhani R,Li YC

    更新日期:2011-03-15 00:00:00

  • P450BM-3: reduction by NADPH and sodium dithionite.

    abstract::Microsomal P450s catalyze the monooxygenation of a large variety of hydrophobic compounds, including drugs, steroids, carcinogens, and fatty acids. The interaction of microsomal P450s with their electron transfer partner, NADPH-P450 reductase, during the transfer of electrons from NADPH to P450, for oxygen activation,...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(92)90738-i

    authors: Peterson JA,Boddupalli SS

    更新日期:1992-05-01 00:00:00

  • Cationomycin and monensin partition between serum proteins and erythrocyte membrane: consequences for Na+ and K+ transport and antimalarial activities.

    abstract::The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1999.1101

    authors: Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

    更新日期:1999-03-15 00:00:00

  • Activation of calcium-sensing receptor by allosteric agonists cinacalcet and AC-265347 abolishes the 1,25(OH)2D3-induced Ca2+ transport: Evidence that explains how the intestine prevents excessive Ca2+ absorption.

    abstract::Long-term high-calcium intake and intestinal calcium hyperabsorption are hazardous to the body. It is hypothesized that enterocytes possess mechanisms for preventing superfluous calcium absorption, including secretion of negative regulators of calcium absorption and utilization of calcium-sensing receptor (CaSR) to de...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2018.09.004

    authors: Wongdee K,Rodrat M,Keadsai C,Jantarajit W,Teerapornpuntakit J,Thongbunchoo J,Charoenphandhu N

    更新日期:2018-11-01 00:00:00

  • HIV and bone disease.

    abstract::Advances in management have resulted in a dramatic decline in mortality for individuals infected with human immunodeficiency virus (HIV). This decrease in mortality, initially the result of improved prophylaxis and treatment of opportunistic infections but later mediated by the use of highly-active antiretroviral ther...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1016/j.abb.2010.07.029

    authors: Stone B,Dockrell D,Bowman C,McCloskey E

    更新日期:2010-11-01 00:00:00

  • Human lactase-phlorizin hydrolase: evidence of dimerization in the endoplasmic reticulum.

    abstract::Human lactase-phlorizin hydrolase [EC 3.2.1.23-3.2.1.62] is a disaccharidase located in the microvillus membrane of small intestinal epithelial cells. The enzyme is synthesized as a precursor protein in the endoplasmic reticulum and in addition to being glycosylated is subsequently proteolytically processed to the mat...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1995.9952

    authors: Grünberg J,Sterchi EE

    更新日期:1995-11-10 00:00:00

  • Up-regulation of hyaluronan synthase genes in cultured human epidermal keratinocytes by UVB irradiation.

    abstract::Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and b...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2007.12.004

    authors: Kakizaki I,Itano N,Kimata K,Hanada K,Kon A,Yamaguchi M,Takahashi T,Takagaki K

    更新日期:2008-03-01 00:00:00

  • The effect of the dilated cardiomyopathy-causing mutation Glu54Lys of alpha-tropomyosin on actin-myosin interactions during the ATPase cycle.

    abstract::In order to understand how the Glu54Lys mutation of alpha-tropomyosin affects actomyosin interactions, we labeled SH1 helix of myosin subfragment-1 (S1) and the actin subdomain-1 with fluorescent probes. These proteins were incorporated into ghost muscle fibers and their conformational states were monitored during the...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2009.07.018

    authors: Borovikov YS,Karpicheva OE,Avrova SV,Robinson P,Redwood CS

    更新日期:2009-09-01 00:00:00

  • Purification and characterization of an acyclic monoterpene primary alcohol:NADP+ oxidoreductase from catmint (Nepeta racemosa).

    abstract::A soluble monoterpene primary alcohol:NADP+ oxidoreductase has been purified to apparent homogeneity from leaves of the catmint, Nepeta racemosa. The purified enzyme consisted of two polypeptides, with molecular masses of 42,000 and 40,000 Da, and contained zinc ions. A number of monoterpene alcohols (geraniol, nerol,...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1995.1210

    authors: Hallahan DL,West JM,Wallsgrove RM,Smiley DW,Dawson GW,Pickett JA,Hamilton JG

    更新日期:1995-04-01 00:00:00

  • Evidence for a novel set of small heat-shock proteins that associates with the mitochondria of murine PC12 cells and protects NADH:ubiquinone oxidoreductase from heat and oxidative stress.

    abstract::Several previously unreported small heat-shock proteins (sHsps) were detected in mitochondria from heat-stressed rat PC12 cells, but not in unstressed controls. Functional inactivation of the mitochondrial sHsps with murine Hsp25 antibody indicated that these sHsps protect NADH:ubiquinone oxidoreductase and NADH dehyd...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1999.1177

    authors: Downs CA,Jones LR,Heckathorn SA

    更新日期:1999-05-15 00:00:00

  • Expression of human plasminogen cDNA in a baculovirus vector-infected insect cell system.

    abstract::A cDNA that encodes the human plasminogen (HPg) amino acid sequence has been inserted adjacent to the polyhedrin promoter in the genome of the baculovirus, Autographa californica nuclear polyhedrosis virus, which was then used to infect cultured cells of the farm armyworm, Spodoptera frugiperda. Under the conditions o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(89)90288-9

    authors: Whitefleet-Smith J,Rosen E,McLinden J,Ploplis VA,Fraser MJ,Tomlinson JE,McLean JW,Castellino FJ

    更新日期:1989-06-01 00:00:00

  • Pectin microgels and their subunit structure.

    abstract::High-performance size exclusion chromatography revealed that alkaline-extracted peach fruit pectin dissolved in 0.05 M NaNO3 comprised a hierarchy of at least four aggregated, macromolecular-sized species. Each of the three largest species was found to be comparable in length to the three smallest subunits of an inter...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(92)90165-s

    authors: Fishman ML,Cooke P,Levaj B,Gillespie DT,Sondey SM,Scorza R

    更新日期:1992-04-01 00:00:00

  • Regulation of subcellular localization of the aryl hydrocarbon receptor (AhR).

    abstract::The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates the toxicity of dioxin and other xenobiotics. In the absence of exogenous ligand, AhR is cytosolic. We investigated how AhR is retained in the cytosol and how dioxin induces AhR to move to the nucleus. Disruption of nuclear ex...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.2001.2339

    authors: Richter CA,Tillitt DE,Hannink M

    更新日期:2001-05-15 00:00:00

  • Electron transport-linked ubiquinone-dependent recycling of alpha-tocopherol inhibits autooxidation of mitochondrial membranes.

    abstract::The objective of this study was to elucidate the anti-oxidative roles of coenzyme Q (CoQ) and alpha-tocopherol in mitochondrial membranes by determining whether CoQ directly scavenges peroxyl- and alkoxyl-radicals or indirectly regenerates alpha-tocopherol during autooxidation of mitochondrial membranes. A comparison ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1997.0606

    authors: Lass A,Sohal RS

    更新日期:1998-04-15 00:00:00

  • Regulation of mRNA stability through a pentobarbital-responsive element.

    abstract::Pentobarbital, a general anesthetic and non-genotoxic carcinogen, can induce gene expression by activating transcription. In the Drosophila glutathione S-transferase D21 (gstD21) gene, pentobarbital's regulatory influence extends to the level of mRNA turnover. Transcribed from an intronless gene, gstD21 mRNA is intrin...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2006.10.026

    authors: Akgül B,Tu CP

    更新日期:2007-03-01 00:00:00

  • Evidence from spin-trapping for a transient radical on tryptophan residue 171 of lignin peroxidase.

    abstract::The heme enzyme lignin peroxidase contains a unique Cbeta-hydroxylated tryptophan residue (Trp171) on the surface of the enzyme. Mutagenetic substitution of Trp171 abolishes completely the veratryl alcohol oxidation activity of the enzyme. This led us to surmise that Trp171 may be involved in electron transfer from na...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1999.1365

    authors: Blodig W,Smith AT,Winterhalter K,Piontek K

    更新日期:1999-10-01 00:00:00

  • Structure-function relationships in heparin cofactor II: spectral analysis of aromatic residues and absence of a role for sulfhydryl groups in thrombin inhibition.

    abstract::This study characterizes the structural and functional significance of sulfhydryl residues in human plasma heparin cofactor II (HCII). For quantification of sulfhydryl groups, the extinction coefficient of HCII was redetermined and found to be 0.593 ml mg-1 cm-1 using second-derivative spectroscopy and multicomponent ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(87)90499-1

    authors: Church FC,Meade JB,Pratt CW

    更新日期:1987-12-01 00:00:00

  • Conversion of dihomo-gamma-linolenic acid to mono- and dihydroxy acids by potato lipoxygenase: evidence for the formation of 8,9-leukotriene A3.

    abstract::Evidence for the formation of a positional isomer of leukotriene (LT) C3 (8,9-LTC3) from dihomo-gamma-linolenic acid has been published (Hammarström, S. J. Biol. Chem. 256, 7712-7714, 1981). This report describes the conversion of dihomo-gamma-linolenic acid to a postulated intermediate in former reaction, 8,9-LTA3, b...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(90)90483-f

    authors: Reddy CC,Bertler C,Hammarström S

    更新日期:1990-06-01 00:00:00

  • Fe-heme structure in Cu, Zn superoxide dismutase from Haemophilus ducreyi by X-ray absorption spectroscopy.

    abstract::We have carried out an X-ray Absorption Spectroscopy (XAS) study of ferric, ferrous, CO- and NO-bound Haemophilus ducreyi Cu,ZnSOD (HdSOD) in solution to investigate the structural modifications induced by the binding of small gaseous ligands to heme in this enzyme. The combined analysis of EXAFS and XANES data has al...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2010.03.010

    authors: D'Angelo P,Zitolo A,Pacello F,Mancini G,Proux O,Hazemann JL,Desideri A,Battistoni A

    更新日期:2010-06-01 00:00:00