Vibrational spectroscopy in the ultraviolet via Gd3+ fluorescence: application to biological systems.

abstract：:The topographical localization of the N-terminus of cytochrome b in the inner mitochondrial membrane was determined by mild proteolysis of the yeast mitochondrial cytochrome bc1 complex and identification of the proteolytic fragments derived from subunits of the complex with an established orientation in the inner mem...

journal_title：Archives of biochemistry and biophysics

authors： Beattie DS,Jenkins HC,Howton MM

更新日期：1994-07-01 00:00:00

abstract：:We previously showed that knockdown of the anaplerotic enzyme pyruvate carboxylase in the INS-1 832/13 insulinoma cell line inhibited glucose-stimulated insulin release and glucose carbon incorporation into lipids. We now show that knockdown of fatty acid synthase (FAS) mRNA and protein also inhibits glucose-stimulate...

journal_title：Archives of biochemistry and biophysics

authors： MacDonald MJ,Hasan NM,Dobrzyn A,Stoker SW,Ntambi JM,Liu X,Sampath H

更新日期：2013-04-01 00:00:00

abstract：:The conversion of 25-hydroxyvitamin D3 to 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) takes place mainly in the kidney and is catalyzed by the enzyme 1alpha-hydroxylase. Parathyroid hormone (PTH) and 1,25-(OH)2D3 are well-known regulators of this tightly controlled step, but the mechanisms by which they modulate 1alpha-hy...

journal_title：Archives of biochemistry and biophysics

authors： Brenza HL,DeLuca HF

更新日期：2000-09-01 00:00:00

abstract：:Characterization of aldose reductase purified from human placenta confirms that activation, as first analyzed in detail for the bovine enzyme, also occurs in humans. Routinely between 5 and 20% of the aldose reductase activity freshly purified from human placenta exhibits kinetic properties and insensitivity to aldose...

journal_title：Archives of biochemistry and biophysics

authors： Grimshaw CE,Lai CJ

更新日期：1996-03-01 00:00:00

abstract：:MicroRNA-342-3p (miR-342) has been shown to act as a tumor-suppressor in different cancer types. However, the role and therapeutic implications of miR-342 via modulation of Cofilin 1 (CFL1) has not been studied in any type of cancer. Given the importance of Cofilin signalling in breast, this study was undertaken to ex...

journal_title：Archives of biochemistry and biophysics

authors： Liu C,Xing H,Luo X,Wang Y

更新日期：2020-07-15 00:00:00

abstract：:Cellobiose dehydrogenase (CDH), an extracellular hemoflavoenzyme produced by the cellulose-degrading cultures of Phanerochaete chrysosporium, oxidizes cellobiose to cellobionolactone. CDH has been purified to homogeneity by a five-step purification procedure. The homogeneous CDH is monomeric and has a relative molecul...

journal_title：Archives of biochemistry and biophysics

authors： Bao W,Usha SN,Renganathan V

更新日期：1993-02-01 00:00:00

abstract：:The neprilysin gene is composed of three distinct 5' noncoding exons which can be joined to the first coding exon to generate multiple mRNA species, all encoding the same protein. Genomic fragments containing upstream sequences of each of these three noncoding exons from the rat neprilysin gene were subcloned in the p...

journal_title：Archives of biochemistry and biophysics

authors： Li C,Hersh LB

更新日期：1998-10-01 00:00:00

abstract：:The fugu (pufferfish) genome has been sequenced, and a second genome assembly was released 17 May 2002. Exhaustive searches were made to identify all P450 genes and pseudogenes from the earlier release of 26 October 2001. P450 genes assembled as completely as possible from these data were used to do additional searche...

journal_title：Archives of biochemistry and biophysics

authors： Nelson DR

更新日期：2003-01-01 00:00:00

abstract：:Since the hepatic LDL receptor is regarded as a major determinant of plasma LDL levels, the effect of diabetes on the expression of this receptor was examined in rat liver. Inducing diabetes with streptozotocin caused a significant reduction in hepatic LDL receptor mRNA levels in concert with an increase in serum chol...

journal_title：Archives of biochemistry and biophysics

authors： Niesen M,Bedi M,Lopez D

更新日期：2008-02-15 00:00:00

abstract：:Pneumococcal surface protein A (PspA) is present on the cell wall of Streptococcus pneumoniae pathogen and has an antigenetically variable N-terminal domain. This aminoterminal domain is essential for full pneumococcal virulence, and monoclonal antibodies raised against it protect mice against pneumococcal infections....

journal_title：Archives of biochemistry and biophysics

authors： Jedrzejas MJ,Hollingshead SK,Lebowitz J,Chantalat L,Briles DE,Lamani E

更新日期：2000-01-01 00:00:00

abstract：:The dicarbonyl compounds, phenylgloxyl and 2,3-butanedione were used to demonstrate the presence of an essential arginine residue in the mechanism of the red beet (Beta vulgaris L.) plasma membrane ATPase. Treatment of the red beet ATPase with either of these reagents resulted in an inhibition of ATP hydrolytic activi...

journal_title：Archives of biochemistry and biophysics

authors： Gildensoph LH,Briskin DP

更新日期：1989-05-15 00:00:00

abstract：:The mechanism of the biosynthesis of 12-oxo-10,15(Z)-phytodienoic acid (12-oxo-PDA) from 13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid in preparations of corn (Zea mays L.) was studied. In the initial reaction the hydroperoxide was converted into an unstable allene oxide, 12,13(S)-epoxy-9(Z),11,15(Z)-octade...

journal_title：Archives of biochemistry and biophysics

authors： Hamberg M,Fahlstadius P

更新日期：1990-02-01 00:00:00

abstract：:The effects of ethanol at concentrations below 10% on the conformation of tropomyosin, its end-to-end polymerization, its binding to F-actin, and its effects on actomyosin ATPase activity were studied. Ethanol stabilized the tropomyosin conformation by shifting the helix thermal unfolding profile to higher temperature...

journal_title：Archives of biochemistry and biophysics

authors： Ishii Y,Lehrer SS,Ohnishi ST,Rubin E

更新日期：1986-05-01 00:00:00

abstract：:Primary chondrocyte cell cultures and explants of bovine articular cartilage were subjected to cyclic hydrostatic pressure in a novel computer-controlled pressure chamber designed for this purpose. The cultures were labeled with 5 microCi/ml 35SO4 and simultaneously pressurized with 5 MPa load for 1.5 or 20 h with pre...

journal_title：Archives of biochemistry and biophysics

authors： Parkkinen JJ,Ikonen J,Lammi MJ,Laakkonen J,Tammi M,Helminen HJ

更新日期：1993-01-01 00:00:00

abstract：:We have analyzed the effect of chlorpromazine (CPZ) on pure actin. We have found that CPZ quenches Trp-79 and Trp-86 fluorescence and, in agreement with an earlier report on conventional actin, inhibits actin polymerization, lowering the extent of polymerization. Moreover, novel polymerization data are presented indic...

journal_title：Archives of biochemistry and biophysics

authors： Milzani A,DalleDonne I

更新日期：1999-09-01 00:00:00

abstract：:Two recombinant sesquiterpene synthases from grand fir, delta-selinene synthase and gamma-humulene synthase, each produce more than 30 sesquiterpene olefins from the acyclic precursor farnesyl diphosphate. These enzymes contain a pair of DDxxD motifs, on opposite lips of the presumptive active site, which are thought ...

journal_title：Archives of biochemistry and biophysics

authors： Little DB,Croteau RB

更新日期：2002-06-01 00:00:00

abstract：:Oxidative treatments of wood pulp lignin by one-electron-abstracting enzymatic or chemical systems result in modification phenomena which are not fully described in terms of those known from lignin model compound studies. The generation of, e.g., long-lived radicals necessitates nondestructive spectroscopic analysis o...

journal_title：Archives of biochemistry and biophysics

authors： Barsberg S

更新日期：2002-08-01 00:00:00

abstract：:Activated leukocytes generate the potent oxidants HOCl and HOBr via the formation of H(2)O(2) and the release of peroxidase enzymes (myeloperoxidase, eosinophil peroxidase). HOCl and HOBr are potent microbiocidal agents, but excessive or misplaced production can cause tissue damage and cell lysis. In this study it is ...

journal_title：Archives of biochemistry and biophysics

authors： Hawkins CL,Brown BE,Davies MJ

更新日期：2001-11-15 00:00:00

abstract：:Treatment with neuronal growth factor (NGF) results in the growth of neuronal processes by PC12 cells and a concomitant 70% increase in the area of the Golgi apparatus. To define the observed morphologic changes in biochemical terms, we investigated the effect of NGF treatment on some Golgi and lysosomal enzyme activi...

journal_title：Archives of biochemistry and biophysics

authors： Rhodes CH,Mezitis SG,Gonatas NK,Fleischer B

更新日期：1989-07-01 00:00:00

abstract：:The adrenal gland synthesizes steroid hormones from the adrenal cortex and catecholamines from the adrenal medulla. Both cortisol and adrenal androgens can have powerful effects on bone. The overproduction of cortisol in Cushing's disease leads to a dramatic reduction in bone density and an increase risk of fracture. ...

journal_title：Archives of biochemistry and biophysics

authors： Hardy R,Cooper MS

更新日期：2010-11-01 00:00:00

abstract：:A 1.1-kb Hp alpha I fragment of the Escherichia coli chromosome containing the gene for translation initiation factor 3 was employed as a probe in heterologous hybridization to chromosomal DNA from a variety of other procaryotes. Positive hybridization was observed to DNA derived from all gram-negative bacteria tested...

journal_title：Archives of biochemistry and biophysics

authors： Pramanik A,Schwartz I

更新日期：1984-11-15 00:00:00

abstract：:Biosynthesis of thyroxine in the thyroid gland involves a reaction between two diiodotyrosyl residues within the same molecule of thyroglobulin, a large, thyroid-specific glycoprotein. This reaction, generally referred to as the coupling reaction, is catalyzed in the thyroid by the heme-containing glycoprotein enzyme,...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris M,Doerge DR

更新日期：1994-11-15 00:00:00

abstract：:Several previously unreported small heat-shock proteins (sHsps) were detected in mitochondria from heat-stressed rat PC12 cells, but not in unstressed controls. Functional inactivation of the mitochondrial sHsps with murine Hsp25 antibody indicated that these sHsps protect NADH:ubiquinone oxidoreductase and NADH dehyd...

journal_title：Archives of biochemistry and biophysics

authors： Downs CA,Jones LR,Heckathorn SA

更新日期：1999-05-15 00:00:00

abstract：:The electron paramagnetic resonance (EPR) signal of the tyrosyl radical attributed to ribonucleoside diphosphate reductase decreases after treatment of promyelocytic leukemic HL60 cells with 2-formylpyridine thiosemicarbazonato copper(II) (CuL). According to EPR studies, CuL forms adducts with both histidine and cyste...

journal_title：Archives of biochemistry and biophysics

authors： Narasimhan J,Antholine WE,Chitambar CR,Petering DH

更新日期：1991-09-01 00:00:00

abstract：:We isolated two new dihydrooxopyridine cross-links, oxodesmosine (OXD) and isooxodesmosine (IOXD) from the acid hydrolysates of the bovine aortic elastin. OXD and IOXD were identified to have N-substituted 1,2-dihydro-2-oxopyridine and N-substituted 1,4-dihydro-4-oxopyridine skeletons, respectively, with three alpha-a...

journal_title：Archives of biochemistry and biophysics

authors： Umeda H,Nakamura F,Suyama K

更新日期：2001-01-01 00:00:00

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:Biochemical communication between the cytoplasmic and mitochondrial subsystems of the cell depends on solute carriers in the mitochondrial inner membrane that transport metabolites between the two compartments. We have expressed and purified a yeast mitochondrial carrier protein (Mtm1p, YGR257cp), originally identifie...

journal_title：Archives of biochemistry and biophysics

authors： Whittaker MM,Penmatsa A,Whittaker JW

更新日期：2015-02-15 00:00:00

abstract：:Production of hydroxyl radicals (HO.) by substrate-supplemented beef heart submitochondrial particles was studied by electron paramagnetic resonance in conjunction with the spin trap 5,5'-dimethyl-1-pirroline-N-oxide (DMPO). Supplementation of submitochondrial particles with NADH or succinate in the presence of antimy...

journal_title：Archives of biochemistry and biophysics

authors： Giulivi C,Boveris A,Cadenas E

更新日期：1995-02-01 00:00:00

abstract：:The thermal denaturation of clathrin-coated vesicles isolated from bovine brain tissue has been studied by differential scanning calorimetry and has been compared to basket structures reformed from isolated triskelion trimers of clathrin and to isolated triskelions. The coated vesicles and reformed baskets displayed s...

journal_title：Archives of biochemistry and biophysics

authors： Schwarz FP,Steer CJ,Kirchhoff WH

更新日期：1989-09-01 00:00:00

abstract：:In the present study, using the C24 bile acid chenodeoxycholic acid as substrate, rat liver bile acid CoA ligase activity (rBAL) was purified 200-fold from detergent-solubilized microsomes using a combination of Q-Sepharose anion exchange, hydroxyapatite, and CM-Sepharose chromatography. Purified rBAL had a molecular ...

journal_title：Archives of biochemistry and biophysics

authors： Wheeler JB,Shaw DR,Barnes S

更新日期：1997-12-01 00:00:00