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The gene encoding translation initiation factor 3 is highly conserved in gram-negative bacteria.

Abstract：

:A 1.1-kb Hp alpha I fragment of the Escherichia coli chromosome containing the gene for translation initiation factor 3 was employed as a probe in heterologous hybridization to chromosomal DNA from a variety of other procaryotes. Positive hybridization was observed to DNA derived from all gram-negative bacteria tested. In contrast, no hybridization to DNA from gram-positive bacteria was detected. In addition, homologous sequences were found in Euglena gracilis chloroplast DNA, while this was not the case with Saccharomyces cerevisiae mitochondrial DNA. These results are discussed in light of existing data on the components and mechanism of translation initiation in the various organisms and organelles employed in this study.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Pramanik A,Schwartz I

doi

10.1016/0003-9861(84)90276-5

subject

Has Abstract

pub_date

1984-11-15 00:00:00

pages

276-82

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(84)90276-5

journal_volume

235

pub_type

杂志文章

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