Reoxygenation injury and antioxidant protection: a tale of two paradoxes.

abstract：:Monoclonal antibodies have been successfully isolated which are isozyme-specific for cytochrome P450p (3A1) or P4501 (3A2), two members of the steroid-inducible cytochrome P450 subfamily exhibiting 89% amino acid sequence homology, and these antibodies show less than 5% cross-reaction with 11 other cytochromes P450 (P...

journal_title：Archives of biochemistry and biophysics

authors： Cooper KO,Reik LM,Jayyosi Z,Bandiera S,Kelley M,Ryan DE,Daniel R,McCluskey SA,Levin W,Thomas PE

更新日期：1993-03-01 00:00:00

abstract：:The oxidized cytochrome c(2) from the purple phototrophic bacteria, Rhodobacter sphaeroides and Rhodobacter capsulatus, bind the neutral species of imidazole (K(a) = 1440 +/- 40 M(-1)) 50 times more strongly than does horse mitochondrial cytochrome c (K(a) = 30 +/- 1 M(-1)). The kinetics of imidazole binding are consi...

journal_title：Archives of biochemistry and biophysics

authors： Dumortier C,Meyer TE,Cusanovich MA

更新日期：1999-11-15 00:00:00

abstract：:Membrane dielectric as a primary basis for effects of ethanol was examined in synaptic plasma membranes (SPM) of genetically selected ethanol-sensitive long-sleep (LS) and ethanol-resistant short-sleep (SS) mice. Multifrequency phase and modulation of fluorometry of diphenylhexatriene (DPH) was used to resolve structu...

journal_title：Archives of biochemistry and biophysics

authors： Schroeder F,Colles SM,Kreishman GP,Heyliger CE,Wood WG

更新日期：1994-03-01 00:00:00

abstract：:The flavoprotein monoamine oxidase B (MAO B) from bovine liver, as isolated, has an unusual additional absorption band at 412 nm, which is similar to the absorption of its anionic flavin semiquinone form, (Fl.-), and other typical (Fl.-) flavoproteins. Denaturation of the enzyme results in the elimination of this anom...

journal_title：Archives of biochemistry and biophysics

authors： Yue KT,Bhattacharyya AK,Zhelyaskov VR,Edmondson DE

更新日期：1993-01-01 00:00:00

abstract：:The relative rates of transcription of the rat liver glutathione S-transferase Ya-Yc and Yb genes were determined in purified liver nuclei isolated at different times after phenobarbital or 3-methylcholanthrene administration. The transcriptional rates of the Ya-Yc and Yb genes were elevated approximately fivefold 8 a...

journal_title：Archives of biochemistry and biophysics

authors： Ding VD,Pickett CB

更新日期：1985-08-01 00:00:00

abstract：:The L chain of the iron storage protein ferritin contains more putative nucleation sites in the core (Glu53, 56, 57, 60, and 63) than does the H chain (Glu61, 64, and 67). Recombinant DNA techniques were used to investigate the role of these putative nucleation sites on iron loading by ceruloplasmin and on the stabili...

journal_title：Archives of biochemistry and biophysics

authors： Juan SH,Aust SD

更新日期：1998-02-15 00:00:00

abstract：:The present paper focussed on the mechanistic aspect of the reaction of singlet oxygen (1O2), in its lowest excited state (1 Delta g), toward DNA constituents. It is well known that 1O2 is able to react with the guanine moiety of DNA to produce almost specifically 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo). Howev...

journal_title：Archives of biochemistry and biophysics

authors： Ravanat JL,Martinez GR,Medeiros MH,Di Mascio P,Cadet J

更新日期：2004-03-01 00:00:00

abstract：:The epithelial cells of the gastrointestinal tract have the capacity to engage in biotransformation of ingested chemicals. A principal component of phase I metabolism of xenobiotics is the family of hemeproteins referred to as cytochrome(s) P450. The presence of cytochrome P450 isoforms was examined by Western blot an...

journal_title：Archives of biochemistry and biophysics

authors： Rosenberg DW

更新日期：1991-01-01 00:00:00

abstract：:Human soluble epoxide hydrolase (hsEH) has been shown to play a role in regulating blood pressure and inflammation. HsEH consists of an N-terminal phosphatase and a C-terminal epoxide hydrolase domain. In the present study, we examined the effects of polymorphisms in the hsEH gene on phosphatase activity, enzyme stabi...

journal_title：Archives of biochemistry and biophysics

authors： Srivastava PK,Sharma VK,Kalonia DS,Grant DF

更新日期：2004-07-15 00:00:00

abstract：:Cancer cells exhibit extreme sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) over normal cells, highlighting TRAIL's potential as a novel and effective cancer drug. However, the therapeutic effect of TRAIL is limited due to drug resistance. In the present study, we sought to investigate ...

journal_title：Archives of biochemistry and biophysics

authors： Wu B,Xiong J,Zhou Y,Wu Y,Song Y,Wang N,Chen L,Zhang J

更新日期：2020-10-15 00:00:00

abstract：:The conversion of the precursor of apolipoprotein A-I (proapoA-I) to apolipoprotein A-I (apoA-I) is known to occur extracellularly by an enzyme that has been shown to be present in plasma. The hepatocarcinoma-derived cell line Hep G2, when grown in culture, secretes proapoA-I. We now show that this cell line also secr...

journal_title：Archives of biochemistry and biophysics

authors： Edelstein C,Kaiser M,Piras G,Scanu AM

更新日期：1988-11-15 00:00:00

abstract：:Bacillus subtilis 168 EpsC is annotated as "Probable polysaccharide biosynthesis protein" in the SwissProt database. epsC is part of the eps operon, thought to be involved in the biosynthesis of exopolymeric substances (EPS). The present study was undertaken to determine the molecular function of EpsC. Sequence analys...

journal_title：Archives of biochemistry and biophysics

authors： Kaundinya CR,Savithri HS,Krishnamurthy Rao K,Balaji PV

更新日期：2018-11-01 00:00:00

abstract：:Stationary phase inocula were more susceptible to the growth inhibitory effect of paraquat than were log phase inocula and this difference was exacerbated in strains overproducing superoxide dismutases (SOD). Glucose-6-phosphate dehydrogenase (G-6-PD), a member of the soxR regulon, was induced by paraquat promptly in ...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：1992-04-01 00:00:00

abstract：:Hitherto the lack of success in cloning the aryl hydrocarbon (Ah) receptor has severely hindered its analysis. The recent cloning of cDNAs for two subunits of the receptor should lead to a renaissance in research. Furthermore, the sequences of the two subunits, as deduced from their cDNA sequences, have shown that the...

journal_title：Archives of biochemistry and biophysics

authors： Hankinson O

更新日期：1993-01-01 00:00:00

abstract：:The oxidation of 2-(methylseleno)benzanilide and 2-selenylbenzanilide, metabolites of the antioxidant drug ebselen, was examined in reactions catalyzed by rat, pig, and guinea pig liver microsomes and in perfused rat liver. Microsomes from all three species catalyzed NADPH- and oxygen-dependent oxidation of the seleni...

journal_title：Archives of biochemistry and biophysics

authors： Akerboom TP,Sies H,Ziegler DM

更新日期：1995-01-10 00:00:00

abstract：:The level of phosphorylation and activation of phosphofructokinase by serotonin (5-hydroxytryptamine) was studied in intact liver flukes Fasciola hepatica. The enzyme was immunoprecipitated with antiserum prepared against pure enzyme from the liver flukes. The resuspended immunoprecipitated enzyme retained most of its...

journal_title：Archives of biochemistry and biophysics

authors： Kamemoto ES,Lan L,Mansour TE

更新日期：1989-06-01 00:00:00

abstract：:We investigated the role of free radicals in hemoglobin (Hb) oxidation and denaturation. To generate free radicals, we used two azocompounds, the hydrophilic 2,2'-azobis(2-amidinopropane hydrochloride and the hydrophobic 2,2'-azobis(2,4-dimethylvaleronitrile) and a drug of the quinone family, phenazine methosulfate. T...

journal_title：Archives of biochemistry and biophysics

authors： Minetti M,Mallozzi C,Scorza G,Scott MD,Kuypers FA,Lubin BH

更新日期：1993-04-01 00:00:00

abstract：:Heparin infusion in an experimental animal model of arterial injury causes a significant increase in the proteoglycan (PG) component of the extracellular matrix in the injured arteries (Snow, A. D., Bolender, R. P., Wight, T. N., and Clowes, A. W. (1990) Am. J. Pathol. 137, 313-330). The mechanisms responsible for thi...

journal_title：Archives of biochemistry and biophysics

authors： Potter-Perigo S,Wight TN

更新日期：1996-12-01 00:00:00

abstract：:Partially purified linamarase from Trifolium repens (genotype Lili acac) plants was kinetically characterized. Kinetic evidence was found to support the assumption that this cyanogenic beta-D-glucosidase has a broad substrate spectrum. p-Nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinopyranoside sub...

journal_title：Archives of biochemistry and biophysics

authors： Pócsi I,Kiss L,Hughes MA,Nánási P

更新日期：1989-08-01 00:00:00

abstract：:Histidine acid phosphatases (HAPs) utilize a nucleophilic histidine residue to catalyze the transfer of a phosphoryl group from phosphomonoesters to water. HAPs function as protein phosphatases and pain suppressors in mammals, are essential for Giardia lamblia excystation, and contribute to virulence of the category A...

journal_title：Archives of biochemistry and biophysics

authors： Dhatwalia R,Singh H,Reilly TJ,Tanner JJ

更新日期：2015-11-01 00:00:00

abstract：:The occurrence of a variety of purine receptors in the immune system indicates that extracellular purines play important functional roles. Extracellular purine concentrations are, in great part, determined by ectonucleotidases, namely, the ATP diphosphohydrolase, also identified as CD39, a lymphocyte cell surface mark...

journal_title：Archives of biochemistry and biophysics

authors： Benrezzak O,Grondin G,Sévigny J,Gendron FP,Rousseau E,D'Orléans-Juste P,Beaudoin AR

更新日期：1999-10-15 00:00:00

abstract：:Cytosolic proteins may play an important role in the intracellular transport of bile acids in enterocytes. The lithocholate binding properties of cytosolic protein from bovine small intestine were studied. Lithocholate binding was observed in the Y (45-50 kDa), Y' (30-35 kDa), and Z fractions (10-15 kDa) following gel...

journal_title：Archives of biochemistry and biophysics

authors： Takikawa H,Arai S,Yamanaka M

更新日期：1992-11-01 00:00:00

abstract：:Superoxide generation, assessed as the rate of acetylated cytochrome c reduction inhibited by superoxide dismutase, by purified NADPH cytochrome P-450 reductase or intact rat liver microsomes was found to account for only a small fraction of their respective NADPH oxidase activities. DTPA-Fe3+ and EDTA-FE3+ greatly st...

journal_title：Archives of biochemistry and biophysics

authors： Morehouse LA,Thomas CE,Aust SD

更新日期：1984-07-01 00:00:00

abstract：:Human hemoglobin (Hb) Coimbra (βAsp99Glu) is one of the seven βAsp99 Hb variants described to date. All βAsp99 substitutions result in increased affinity for O2 and decreased heme-heme cooperativity and their carriers are clinically characterized by erythrocytocis, caused by tissue hypoxia. Since βAsp99 plays an impor...

journal_title：Archives of biochemistry and biophysics

authors： Jorge SE,Bringas M,Petruk AA,Arrar M,Marti MA,Skaf MS,Costa FF,Capece L,Sonati MF,Estrin D

更新日期：2018-01-01 00:00:00

abstract：:A class II Zn(2+)-dependent fructose-1,6-bisphosphate (FBP)- aldolase was purified from an overproducer strain of Escherichia coli and characterized by standard biochemical techniques and 13C NMR spectroscopy. The principal finding of these studies was identification, by 13C NMR spectroscopy, of an enzyme-bound reacti...

journal_title：Archives of biochemistry and biophysics

authors： Szwergold BS,Ugurbil K,Brown TR

更新日期：1995-02-20 00:00:00

abstract：:Both CYP158A1 and CYP158A2 are able to catalyze an oxidative C-C coupling reaction producing biflaviolin or triflaviolin in Streptomyces coelicolor A3(2). The substrate-bound crystal structures of CYP158A2 and CYP158A1 reveal that the side chain of Ile87 in CYP158A2 points to the active site contacting the distal flav...

journal_title：Archives of biochemistry and biophysics

authors： Zhao B,Bellamine A,Lei L,Waterman MR

更新日期：2012-02-15 00:00:00

abstract：:Chronic kidney disease (CKD) is an increasingly recognized burden for patients and health care systems with high (and growing) global incidence and prevalence, significant mortality, and disproportionately high treatment costs. Yet, the available diagnostic tools are either impractical in clinical routine or have seri...

journal_title：Archives of biochemistry and biophysics

authors： Breit M,Weinberger KM

更新日期：2016-01-01 00:00:00

abstract：:Imidazolium trans-imidazoledimethylsulfoxide-tetrachlororuthenate (NAMI-A) is a new ruthenium compound active against lung metastasis in vivo and tumor cell invasion in vitro. Since angiogenesis was recognized as a key event in the metastasizing process, the manipulation of neo-vessel formation has been developed as a...

journal_title：Archives of biochemistry and biophysics

authors： Sanna B,Debidda M,Pintus G,Tadolini B,Posadino AM,Bennardini F,Sava G,Ventura C

更新日期：2002-07-15 00:00:00

abstract：:The cardiomyopathy produced by the widely used anticancer drug adriamycin (ADR) is believed to be related to the production of reaction oxygen species and consumption of reduced glutathione (GSH) during redox cycling of the drug. Protection by vitamin E against the toxicity of ADR was studied in a model of compromised...

journal_title：Archives of biochemistry and biophysics

authors： Pascoe GA,Reed DJ

更新日期：1987-07-01 00:00:00

abstract：:Long-term high-calcium intake and intestinal calcium hyperabsorption are hazardous to the body. It is hypothesized that enterocytes possess mechanisms for preventing superfluous calcium absorption, including secretion of negative regulators of calcium absorption and utilization of calcium-sensing receptor (CaSR) to de...

journal_title：Archives of biochemistry and biophysics

authors： Wongdee K,Rodrat M,Keadsai C,Jantarajit W,Teerapornpuntakit J,Thongbunchoo J,Charoenphandhu N

更新日期：2018-11-01 00:00:00