Role of oxygen and carbon radicals in hemoglobin oxidation.

abstract：:An NAD-preferring glucose 6-phosphate dehydrogenase of Acetobacter hansenii (formerly known as Acetobacter xylinum) has been purified to apparent homogeneity and kinetically characterized. The purified enzyme was stabilized by the use of glycerol, MgSO4, and 2-mercaptoethanol at pH 5.4. The molecular weight of the enz...

journal_title：Archives of biochemistry and biophysics

authors： Ragunathan S,Levy HR

更新日期：1994-05-01 00:00:00

abstract：:An in vitro assay that measures endosome fusion was used to characterize the role of guanosine triphosphate (GTP)-binding proteins in endocytosis. Guanosine 5',3-(thio)triphosphate (GTP gamma S), a nonhydrolyzable analog of GTP, stimulates the binding of cytosolic factors to the endosomal membrane (priming). GTP gamma...

journal_title：Archives of biochemistry and biophysics

authors： Lenhard JM,Colombo MI,Stahl PD

更新日期：1994-08-01 00:00:00

abstract：:Western blots using a polyclonal and a monoclonal antibody raised against rat liver cytochrome P-450b indicate tissue-specific expression of low levels of cytochrome P-450's b and e. P-450b and P-450e were expressed very selectively in, respectively, lung and adrenal microsomes of untreated rats but neither isozyme wa...

journal_title：Archives of biochemistry and biophysics

authors： Christou M,Wilson NM,Jefcoate CR

更新日期：1987-11-01 00:00:00

abstract：:Two complementary DNA (cDNA) clones (pTK-1 and -2) encoding two distinct isotypes of mouse Mg(2+)-dependent protein phosphatase beta (MPP beta-1 and -2, respectively) were isolated from a melanocyte cDNA library. Although mouse pTK-1 is orthologous to the rat cDNA (JW5) reported previously [Wenk, J., Trompeter, H.I., ...

journal_title：Archives of biochemistry and biophysics

authors： Terasawa T,Kobayashi T,Murakami T,Ohnishi M,Kato S,Tanaka O,Kondo H,Yamamoto H,Takeuchi T,Tamura S

更新日期：1993-12-01 00:00:00

abstract：:Inactivation of the von Hippel-Lindau (VHL) tumor suppressor is associated with renal carcinoma, hemangioblastoma and pheochromocytoma. The VHL protein is a component of a ubiquitin ligase complex that ubiquitinates and degrades hypoxia inducible factor-α (HIF-α). Degradation of HIF-α by VHL is proposed to suppress tu...

journal_title：Archives of biochemistry and biophysics

authors： Lai Y,Song M,Hakala K,Weintraub ST,Shiio Y

更新日期：2012-02-15 00:00:00

abstract：:Rabbit anti-tyrosinase antibodies were used to study the abundance, processing, and degradation of tyrosinase in murine (Cloudman) melanoma cells. The polyclonal antibodies precipitated low-molecular-weight (68,000 and 70,000) and high-molecular-weight (78,000 and 80,000) tyrosinases that had a precursor-product relat...

journal_title：Archives of biochemistry and biophysics

authors： Halaban R,Pomerantz SH,Marshall S,Lerner AB

更新日期：1984-04-01 00:00:00

abstract：:A 1.1-kb Hp alpha I fragment of the Escherichia coli chromosome containing the gene for translation initiation factor 3 was employed as a probe in heterologous hybridization to chromosomal DNA from a variety of other procaryotes. Positive hybridization was observed to DNA derived from all gram-negative bacteria tested...

journal_title：Archives of biochemistry and biophysics

authors： Pramanik A,Schwartz I

更新日期：1984-11-15 00:00:00

abstract：:The effects of temperature on the initial velocity kinetics of allosteric ATP sulfurylase from Penicillium chrysogenum were measured. The experiments were prompted by the structural similarity between the C-terminal regulatory domain of fungal ATP sulfurylase and fungal APS kinase, a homodimer that undergoes a tempera...

journal_title：Archives of biochemistry and biophysics

authors： Medina DC,Hanna E,MacRae IJ,Fisher AJ,Segel IH

更新日期：2001-09-01 00:00:00

abstract：:Cytochrome P450 genes (CYPs) encoding two new subfamilies designated CYP4X1 and CYP4Z1 were identified in the human genome and the Expressed Sequence Tags database. Partial cDNAs encoding both P450s were isolated from human kidney and used to determine tissue distribution. CYP4X1 was predominantly expressed in trachea...

journal_title：Archives of biochemistry and biophysics

authors： Savas U,Hsu MH,Griffin KJ,Bell DR,Johnson EF

更新日期：2005-04-15 00:00:00

abstract：:Experimental and computational studies suggest that few general principles govern protein/protein interactions and aggregation. The knowledge of these rules may be exploited to design peptides that are able to interfere with the self-assembly and aggregation of proteins. This work is aimed to verify the validity of th...

journal_title：Archives of biochemistry and biophysics

authors： La Rosa C,Milardi D,Amato E,Pappalardo M,Grasso D

更新日期：2005-03-01 00:00:00

abstract：:We have studied the time-resolved fluorescence of three engineered Tet repressor (TetR) mutants bearing a single Trp residue at positions 162, 163, and 165 in the C-terminal part of the loop joining helices 8 and 9. Detailed analysis indicates that, at 20 degrees, the fluorescence decay of each Trp can be described as...

journal_title：Archives of biochemistry and biophysics

authors： Alberti P,Bombarda E,Kintrup M,Hillen W,Lami H,Piémont E,Doglia SM,Chabbert M

更新日期：1997-10-15 00:00:00

abstract：:The nucleotide sequence of tyrosine tRNAQ* psi A from bovine liver was determined to be pC-C-U-U-C-m2G-A-U-A-m2G-C-U-C-A-G-D-D-G-G-acp3U-A-G-A -G-C-m22G-m22G -A-G-G-A-C-U-Q*-psi-A-m1G-A-psi m-C-C-U-U-A-G-m7G-D-m5C-G-C-U-G-G-T-psi-C-G-m1A -U-U-C-C-G-G-C-U-C-G-A-A-G-G-A-C-C-AOH. This tyrosine tRNA is 76 nucleotides in l...

journal_title：Archives of biochemistry and biophysics

authors： Johnson GD,Pirtle IL,Pirtle RM

更新日期：1985-01-01 00:00:00

abstract：:The in vivo stability of ferredoxin-nitrate reductase from the cyanobacterium Anacystis nidulans under conditions of inhibited protein synthesis has been studied in nitrate-grown cells. A light-promoted rapid decay in cellular nitrate reductase activity took place in the absence of any added nitrogen source, but not i...

journal_title：Archives of biochemistry and biophysics

authors： Herrero A,Flores E,Guerrero MG

更新日期：1984-11-01 00:00:00

abstract：:The metabolic fates of NG,NG-and NG,N'G-dimethylarginines in rats were investigated isotopically and novel metabolites, alpha-keto-delta-(N,N-dimethylguanidino)-and alpha-keto-delta-(N,N'-dimethylguanidino)valeric acids and gamma-(N,N-dimethylguanidino)-and gamma-(N,N'-dimethylguanidino)butyric acids were identified. ...

journal_title：Archives of biochemistry and biophysics

authors： Ogawa T,Kimoto M,Watanabe H,Sasaoka K

更新日期：1987-02-01 00:00:00

abstract：:The single-stranded, DNA-dependent ATPase activity of purified recA protein was found to be inhibited competitively by ribose-modified analogs of ATP, 3'-O-anthraniloyl-ATP (Ant-ATP), and 3'-O-(N-methylanthraniloyl)-ATP (Mant-ATP). The Ki values for Ant-ATP and Mant-ATP were around 7 and 3 microM at pH 7.5, respective...

journal_title：Archives of biochemistry and biophysics

authors： Karasaki Y,Higashi K

更新日期：1984-09-01 00:00:00

abstract：:Treatment of human pulmonary artery smooth muscle cells (HPASMCs) with the thromboxane A2 receptor antagonist, SQ29548 inhibited U46619 stimulation of phospholipase D (PLD) and NADPH oxidase activities in the cell membrane. Pretreatment with apocynin inhibited U46619 induced increase in NADPH oxidase activity. The cel...

journal_title：Archives of biochemistry and biophysics

authors： Chakraborti S,Sarkar J,Chowdhury A,Chakraborti T

更新日期：2017-11-01 00:00:00

abstract：:A set of membrane proteins from murine myelomonocytic leukemia cell line WEHI-3BD+ was found to be ADP-ribosylated. Both this ADP-ribosylation reaction and granulocytic differentiation in response to recombinant G-CSF were inhibited approximately 50% by 2 mM benzamide, suggesting a correlation between these two proces...

journal_title：Archives of biochemistry and biophysics

authors： Hanson K,Aboul-Ela N,Jacobson MK,Wu MC

更新日期：1993-04-01 00:00:00

abstract：:Polycation binding to the negatively charged surface of chloroplast thylakoid membranes is known to cause an inhibition of photosystem I activity. It also interferes with the cation-dependent rearrangement of chlorophyll proteins in the thylakoid membrane. It was shown that added anions prevented or reversed the inhib...

journal_title：Archives of biochemistry and biophysics

authors： Richter ML,Homann PH

更新日期：1983-04-01 00:00:00

abstract：:Benzimidazole compounds, such as omeprazole and thiabendazole, are a different type of CYP1A1 inducer from Ah receptor-ligands, such as TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and 3-methylcholanthrene. In HepG2 cells, the commonly used tyrosine kinase inhibitors, herbimycin-A and a series of tyrphostins, inhibited ...

journal_title：Archives of biochemistry and biophysics

authors： Kikuchi H,Hossain A,Yoshida H,Kobayashi S

更新日期：1998-10-15 00:00:00

abstract：:Much has been written recently on osteoclast biology, but this cell type still astonishes scientists with its multifaceted functions and unique properties. The last three decades have seen a change in thinking about the osteoclast, from a cell with a single function, which just destroys the tissue it belongs to, to an...

journal_title：Archives of biochemistry and biophysics

authors： Cappariello A,Maurizi A,Veeriah V,Teti A

更新日期：2014-11-01 00:00:00

abstract：:Isolated mitochondria loaded with Ca(2+) and then exposed to Fe(2+) show a transient release of Ca(2+). The magnitude of this response depends on the Ca(2+) loading and the kinetics of the response depends on the concentration of added Fe(2+). We investigated the Fe(2+)-induced Ca(2+) release mechanism by measuring mi...

journal_title：Archives of biochemistry and biophysics

authors： Gogvadze V,Walter PB,Ames BN

更新日期：2002-02-15 00:00:00

abstract：:The effects of insulin on phospholipid metabolism and generation of diacylglycerol (DAG) and on activation of protein kinase C in rat hepatocytes were compared to those of vasopressin and angiotension II. Insulin provoked increases in [3H]glycerol labeling of phosphatidic acid (PA), diacylglycerol (DAG), and other gly...

journal_title：Archives of biochemistry and biophysics

authors： Cooper DR,Hernandez H,Kuo JY,Farese RV

更新日期：1990-02-01 00:00:00

abstract：:In bovine retinal pigment epithelium membranes we have found three hydrolases which were active against trans-retinyl palmitate. This was possible by assaying different subcellular fractions as a function of pH in the range 3-9. Detection of these activities has been favored by the use in the enzyme assay of Triton X-...

journal_title：Archives of biochemistry and biophysics

authors： Gueli MC,Nicotra CM,Pintaudi AM,Paganini A,Pandolfo L,De Leo G,Di Bella MA

更新日期：1991-08-01 00:00:00

abstract：:A new procedure for the purification of phospholipase C from Clostridium perfringens has been devised that results in essentially pure enzyme. The procedure consists of ammonium sulfate fractionation, ion-exchange chromatography on QAE-Sephadex, and affinity chromatography on phosphatidylcholine linked to Sepharose. T...

journal_title：Archives of biochemistry and biophysics

authors： Krug EL,Kent C

更新日期：1984-06-01 00:00:00

abstract：:Two N-terminal fusion proteins combining Escherichia coli maltose-binding protein (MBP) and the 12-transmembrane-segment pBR322 tetracycline resistance protein (Tet) have been constructed to determine the strength and location of topology control signals within the N-terminal portion of the Tet protein. The fusions co...

journal_title：Archives of biochemistry and biophysics

authors： Miller KW,Jewell JE

更新日期：1995-10-01 00:00:00

abstract：:A kinetic study of acetaminophen oxidation by tyrosinase in the presence of a physiological nucleophilic agent such as the amino acid L-proline is performed in the present paper. The o-quinone product of the catalytic activity, 4-acetamido-o-benzoquinone, becomes unstable through the chemical addition of L-proline, in...

journal_title：Archives of biochemistry and biophysics

authors： Valero E,Varón R,García-Carmona F

更新日期：2003-08-15 00:00:00

abstract：:DNA of livers or kidneys of rodents contain endogenously modified nucleotides of as yet unknown structure or function. As part of our examination of the origin of endogenous DNA modifications, we examined polar adducts of hamster liver and kidney DNA by 32P-postlabeling analysis (central and upper cuts) and compared t...

journal_title：Archives of biochemistry and biophysics

authors： Wang MY,Liehr JG

更新日期：1995-01-10 00:00:00

abstract：:Vitamin E (vE) is a biological free radical scavenger capable of providing antioxidant protection depending upon its tissue content. In previous studies, we observed that vE increased significantly in rat lungs after oxidant exposure, and we postulated that vE may be mobilized to the lung from other body sites under o...

journal_title：Archives of biochemistry and biophysics

authors： Elsayed NM,Mustafa MG,Mead JF

更新日期：1990-11-01 00:00:00

abstract：:Xenopus laevis nucleoplasmin is a pentameric nuclear chaperone. The relation between the structure and the multifunctional aspects of the molecule has not yet been clearly established. In the course of analysing a C-terminally His-tagged recombinant version of the region equivalent to the trypsin resistant core (r-NP1...

journal_title：Archives of biochemistry and biophysics

authors： Arnan C,Prieto C,Chiva M,Salvany L,Ausió J,Subirana JA,Saperas N

更新日期：2005-05-15 00:00:00

abstract：:Human leukemic T-lymphocytes undergo extensive and rapid apoptosis in the presence of L1AD3, a small cyclic peptide derivative of cobra cardiotoxin. The first step in this process involves its binding to membranes of susceptible cells. By the use of a biotin "handle" synthetically incorporated at the N-terminus of L1A...

journal_title：Archives of biochemistry and biophysics

authors： Smith CA,Hinman CL

更新日期：2004-12-01 00:00:00