Inhibition of ATPase activity of the recA protein by ATP ribose-modified analogs.

abstract：:It was shown previously that the ATP synthase complex of bovine heart mitochondria contains an essential set of thiols or dithiols in its membrane sector (F0), whose modification by various reagents results in uncoupling [Yagi, T., and Hatefi, Y. (1984) Biochemistry 23, 2449-2455]. The sensitivity to modifiers was inc...

journal_title：Archives of biochemistry and biophysics

authors： Yagi T,Hatefi Y

更新日期：1987-04-01 00:00:00

abstract：:Paxilline, an indole alkaloid mycotoxin from Penicillium paxilli, is an inhibitor of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA). Paxilline inhibited differing isoforms of SERCA with IC50s between 5 and 50 microM. It inhibited more potently the purified Ca2+ ATPase activity from skeletal muscle with an IC50 of...

journal_title：Archives of biochemistry and biophysics

authors： Bilmen JG,Wootton LL,Michelangeli F

更新日期：2002-10-01 00:00:00

abstract：:Aryl hydrocarbon hydroxylase activity is most closely associated with cytochrome P-450c in the rat and cytochrome P1-450 in the mouse. The sequence for the orthologous human gene coding for this enzymatic activity has been determined from several sources: cytochrome P-450c isolated from human embryonic DNA [K. Kawajir...

journal_title：Archives of biochemistry and biophysics

authors： Iversen PL,Heiger WJ,Bresnick E,Hines RN

更新日期：1987-07-01 00:00:00

abstract：:The crystalline beef liver protein of Sumner and Dounce (A. L. Dounce, P. Z. Allen, and G. A. Mourtzikos (1978) Arch. Biochem. Biophys. 188, 251-265) termed FTBL (football) protein because of the shape of its crystals, has been identified as a crystalline leucine aminopeptidase (LAP), on the basis of its high specific...

journal_title：Archives of biochemistry and biophysics

authors： Dounce AL,Allen PZ

更新日期：1987-08-15 00:00:00

abstract：:The effects of extracellular ATP on phosphoinositide metabolism and intracellular Ca2+ homeostasis were studied in Ehrlich ascites tumor cells. Cytosolic [Ca2+] was measured using either quin 2 or the recently described indicator fura 2. Addition of 0.5-25 microM extracellular ATP to intact cells results in a rapid mo...

journal_title：Archives of biochemistry and biophysics

authors： Dubyak GR

更新日期：1986-02-15 00:00:00

abstract：:Ca2+ binding and internalization in sarcoplasmic reticulum ATPase can be investigated by the use of La3+ as a Ca2+ analog. Displacement kinetics of Ca2+ bound by La3+ in native vesicles is a slow biphasic process (k1 = 0.55 s-1 and k2 = 0.05 s-1) that is consistent with the existence of two Ca2+ binding populations wh...

journal_title：Archives of biochemistry and biophysics

authors： Fernandez-Belda F

更新日期：1988-12-01 00:00:00

abstract：:Sixteen mutants of Escherichia coli defective in H+-ATPase (proton-translocating ATPase) were tested for their ability to recombine with hybrid plasmids carrying various portions of the beta subunit cistron. Twelve mutations were mapped within the carboxyl half of the cistron corresponding to amino acid residues 279 t...

journal_title：Archives of biochemistry and biophysics

authors： Kanazawa H,Noumi T,Oka N,Futai M

更新日期：1983-12-01 00:00:00

abstract：:Salicylhydroxamic acid reductase, which catalyzes the reduction of salicylhydroxamic acid to salicylamide, was purified from rat liver cytosol. The purification procedure consisted of fractionation with ammonium sulfate, chromatography with Phenyl-Toyopearl 650, DEAE-cellulose, hydroxyapatite, and Sephadex G-200, and ...

journal_title：Archives of biochemistry and biophysics

authors： Katsura H,Kitamura S,Tatsumi K

更新日期：1993-05-01 00:00:00

abstract：:The regulation of the plasma membrane Ca2+ pump by hormones via phosphorylation in intact cells has not been clearly established. We now present evidence that the Ca2+ pump is phosphorylated on both serine and threonine residues in unstimulated and stimulated cultured rat aortic endothelial cells. Among the stimuli te...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Du YS,Diglio C,Tsang W,Kuo TH

更新日期：1991-08-15 00:00:00

abstract：:Advancements in peptide fusion technologies to maximize the protein production has taken a big leap to fulfill the demands of post-genomics era targeting elucidation of structure/function of the proteome and its therapeutic applications, by over-expression in heterologous expression systems. Despite being most preferr...

journal_title：Archives of biochemistry and biophysics

authors： Yadav DK,Yadav N,Yadav S,Haque S,Tuteja N

更新日期：2016-12-15 00:00:00

abstract：:The protein sequence encoded by a creatine transporter cDNA cloned from a human heart library was identical to that cloned from a human kidney library (Nash et al., Receptors Channels 2, 165-174, 1994), except that at position 285 the former contained an Ala residue and the latter contained a Pro residue. Expression o...

journal_title：Archives of biochemistry and biophysics

authors： Dai W,Vinnakota S,Qian X,Kunze DL,Sarkar HK

更新日期：1999-01-01 00:00:00

abstract：:Numerous conditions induce expression of manganese-containing superoxide dismutase (MnSOD) in mammalian cells. The reported inducers of MnSOD are all agents that activate two transcription factors, AP-1 and NF kappa B, but several reports have suggested that MnSOD induction relies solely on NF kappa B. We investigated...

journal_title：Archives of biochemistry and biophysics

authors： Borrello S,Demple B

更新日期：1997-12-15 00:00:00

abstract：:The zaragozic acids are potent inhibitors of squalene synthase. In vivo studies in mice confirmed our earlier observations that inhibition of squalene synthase by zaragozic acid A was accompanied by an increase in the incorporation of label from [3H]mevalonate into farnesyl-diphosphate (FPP)-derived isoprenoic acids (...

journal_title：Archives of biochemistry and biophysics

authors： Vaidya S,Bostedor R,Kurtz MM,Bergstrom JD,Bansal VS

更新日期：1998-07-01 00:00:00

abstract：:Using fractionation procedures such as sucrose gradient sedimentation and gel permeation chromatography, a novel cytosolic binding protein for benzo[a]pyrene has been detected in liver of nonmammalian and mammalian species including human. This protein, called 8 S protein, cosediments with the Ah receptor after centri...

journal_title：Archives of biochemistry and biophysics

authors： Lesca P,Peryt B,Soues S,Maurel P,Cravedi JP

更新日期：1993-05-15 00:00:00

abstract：:Escherichia coli pyruvate oxidase (POXEC) requires FAD both for the oxidative decarboxylation of pyruvate to acetate and CO2 and for the formation of acetoin from pyruvate and acetaldehyde. Prior work has shown that the catalytic activity (kcat/Km) for POXEC in the oxidative reaction is stimulated approximately 450-fo...

journal_title：Archives of biochemistry and biophysics

authors： Bertagnolli BL,Hager LP

更新日期：1993-01-01 00:00:00

abstract：:Recently our group synthesized a new class of melanins obtained by the tyrosinase-catalyzed oxidation of opioid peptides (opiomelanins). Owing to the presence of the peptide moiety such pigments exhibit high solubility in hydrophilic solvents, which allows spectroscopic investigations. In particular, the absence of so...

journal_title：Archives of biochemistry and biophysics

authors： Mosca L,De Marco C,Fontana M,Rosei MA

更新日期：1999-11-01 00:00:00

abstract：:A class II Zn(2+)-dependent fructose-1,6-bisphosphate (FBP)- aldolase was purified from an overproducer strain of Escherichia coli and characterized by standard biochemical techniques and 13C NMR spectroscopy. The principal finding of these studies was identification, by 13C NMR spectroscopy, of an enzyme-bound reacti...

journal_title：Archives of biochemistry and biophysics

authors： Szwergold BS,Ugurbil K,Brown TR

更新日期：1995-02-20 00:00:00

abstract：:Rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was inactivated by peroxynitrite under biologically relevant conditions. The decrease of enzymatic activity followed an exponential function, and the concentration of peroxynitrite needed to inactivate 50% of 7 microM GAPDH (IC50) was 17 microM. Hydroxyl r...

journal_title：Archives of biochemistry and biophysics

authors： Souza JM,Radi R

更新日期：1998-12-15 00:00:00

abstract：:The reaction of the Cu,Co derivative of bovine Cu,Zn superoxide dismutase with phenylglyoxal or butanedione, which are known to inactivate the enzyme by selectively binding to Arg 141, has been studied by 1H NMR. Several 1H NMR lines of the copper-liganding histidine residues were perturbed, reproducing an effect so f...

journal_title：Archives of biochemistry and biophysics

authors： Paci M,Desideri A,Sette M,Rotilio G

更新日期：1991-04-01 00:00:00

abstract：:If liver alcohol dehydrogenase were rate-limiting in ethanol metabolism, inhibitors of the enzyme should inhibit the metabolism with the same type of kinetics and the same kinetic constants in vitro and in vivo. Against varied concentrations of ethanol, 4-methylpyrazole is a competitive inhibitor of purified rat liver...

journal_title：Archives of biochemistry and biophysics

authors： Plapp BV,Leidal KG,Smith RK,Murch BP

更新日期：1984-04-01 00:00:00

abstract：:Two new members of the polyketide synthase (PKS) gene family (RiPKS4 and RiPKS5) were cloned from raspberry fruits (Rubus idaeus L., cv Royalty) and expressed in Escherichia coli. Characterization of the recombinant enzyme products indicated that RiPKS4 is a bifunctional polyketide synthase producing both 4-hydroxyben...

journal_title：Archives of biochemistry and biophysics

authors： Zheng D,Hrazdina G

更新日期：2008-02-15 00:00:00

abstract：:Modification/chlorination of high-density lipoprotein (HDL) by hypochlorous acid (HOCl), formed by the myeloperoxidase-H₂O₂-chloride system of activated phagocytes, converts an anti-atherogenic lipoprotein into a pro-inflammatory lipoprotein particle. Chlorinated HDL is present in human lesion material, binds to and i...

journal_title：Archives of biochemistry and biophysics

authors： Rossmann C,Rauh A,Hammer A,Windischhofer W,Zirkl S,Sattler W,Malle E

更新日期：2011-05-01 00:00:00

abstract：:Rats were fed toxic levels of methionine with or without simultaneous dietary supplements of glycine and serine. Feed intake, growth rate, and metabolite concentrations in intestine, plasma, liver, skeletal muscle, and kidneys were monitored. Both toxic amounts of methionine and supplemental glycine and serine affecte...

journal_title：Archives of biochemistry and biophysics

authors： Regina M,Korhonen VP,Smith TK,Alakuijala L,Eloranta TO

更新日期：1993-02-01 00:00:00

abstract：:The primary site of action for the novel antibiotic, bicyclomycin, in Escherichia coli has been identified to be the rho transcription termination factor. The inhibition of rho poly(C)-stimulated hydrolysis of ATP by bicyclomycin has been found to proceed by a non-competitive, reversible pathway with respect to ATP (K...

journal_title：Archives of biochemistry and biophysics

authors： Park HG,Zhang X,Moon HS,Zwiefka A,Cox K,Gaskell SJ,Widger WR,Kohn H

更新日期：1995-11-10 00:00:00

abstract：:A marine snail digestive phospholipase A2 (mSDPL) was purified from delipidated hepatopancreas. Unlike known digestive phospholipases A2, which are 14 kDa proteins, the purified mSDPL has a molecular mass of about 30 kDa. It has a specific activity of about 180 U/mg measured at 50 degrees C and pH 8.5 using phosphatid...

journal_title：Archives of biochemistry and biophysics

authors： Zarai Z,Bacha AB,Horchani H,Bezzine S,Zouari N,Gargouri Y,Mejdoub H

更新日期：2010-02-15 00:00:00

abstract：:The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

journal_title：Archives of biochemistry and biophysics

authors： Salvador JM,Mata AM

更新日期：1998-03-15 00:00:00

abstract：:In the presence of glucose and ample oxygen, insect form African trypanosomes release pyruvate more than 100-fold more slowly than do bloodstream forms. This rate decrease could not be accounted for simply by an increased mitochondrial pyruvate oxidation rate as inhibiting mitochondrial respiration increases pyruvate ...

journal_title：Archives of biochemistry and biophysics

authors： Barnard JP,Pedersen PL

更新日期：1994-08-15 00:00:00

abstract：:The effect of ligating the alpha2-macroglobulin signaling receptor (alpha2MSR) with receptor-recognized forms of alpha2M (alpha2M*) was studied with respect to phospholipase D (PLD) activity in murine macrophages, their plasma membranes, and nuclei. PLD activity in plasma membranes and nuclei increased linearly up to ...

journal_title：Archives of biochemistry and biophysics

authors： Misra UK,Pizzo SV

更新日期：1999-03-01 00:00:00

abstract：:Recombinant ethanolamine ammonia-lyase from S. typhimurium has been overexpressed and purified in large quantities by a simple procedure. The molecular weight of the native enzyme is about 480 kDa, and it contains two active sites/molecule as shown by kinetic studies and by titration with CNCbl. Analysis by sodium dod...

journal_title：Archives of biochemistry and biophysics

authors： Faust LP,Babior BM

更新日期：1992-04-01 00:00:00

abstract：:Biochemical communication between the cytoplasmic and mitochondrial subsystems of the cell depends on solute carriers in the mitochondrial inner membrane that transport metabolites between the two compartments. We have expressed and purified a yeast mitochondrial carrier protein (Mtm1p, YGR257cp), originally identifie...

journal_title：Archives of biochemistry and biophysics

authors： Whittaker MM,Penmatsa A,Whittaker JW

更新日期：2015-02-15 00:00:00