Catalytic oxidation of acetaminophen by tyrosinase in the presence of L-proline: a kinetic study.

abstract：:If liver alcohol dehydrogenase were rate-limiting in ethanol metabolism, inhibitors of the enzyme should inhibit the metabolism with the same type of kinetics and the same kinetic constants in vitro and in vivo. Against varied concentrations of ethanol, 4-methylpyrazole is a competitive inhibitor of purified rat liver...

journal_title：Archives of biochemistry and biophysics

authors： Plapp BV,Leidal KG,Smith RK,Murch BP

更新日期：1984-04-01 00:00:00

abstract：:High-performance size exclusion chromatography revealed that alkaline-extracted peach fruit pectin dissolved in 0.05 M NaNO3 comprised a hierarchy of at least four aggregated, macromolecular-sized species. Each of the three largest species was found to be comparable in length to the three smallest subunits of an inter...

journal_title：Archives of biochemistry and biophysics

authors： Fishman ML,Cooke P,Levaj B,Gillespie DT,Sondey SM,Scorza R

更新日期：1992-04-01 00:00:00

abstract：:The fertilization layer of Xenopus laevis eggs is formed by the cortical granule lectin binding to its ligand. The binding requires Ca2+, is specific for galactose, and functionally establishes a block to polyspermy at fertilization. We have designed a new enzyme-linked lectin assay for the cortical granule lectin (CG...

journal_title：Archives of biochemistry and biophysics

authors： Quill TA,Hedrick JL

更新日期：1996-09-15 00:00:00

abstract：:Eleven hybridoma clones which secrete monoclonal antibodies against purified rat liver alcohol dehydrogenase (EC 1.1.1.1) were isolated. Antibodies (R-1-R-11) were identified by their ability to bind to immobilized pure alcohol dehydrogenase in an enzyme-linked immunoadsorbent assay, in which antibody R-9 showed the h...

journal_title：Archives of biochemistry and biophysics

authors： Lad PJ,Schenk DB,Leffert HL

更新日期：1984-12-01 00:00:00

abstract：:Cocoa flavonoids are able to reduce cardiovascular risk by improving endothelial function and decreasing blood pressure (BP). Interest in the biological activities of cocoa is daily increasing. A recent meta-analysis shows flavanol-rich cocoa administration decreases mean systolic (-4.5mm Hg; p<0.001) and diastolic (-...

journal_title：Archives of biochemistry and biophysics

authors： Grassi D,Desideri G,Ferri C

更新日期：2010-09-01 00:00:00

abstract：:Methionine is oxidized to methionine sulfoxide by rat liver and kidney microsomes in an O2- and NADPH-dependent manner. In all microsomal assays, no methionine sulfone was detected. Use of a monoclonal antibody to rat liver cytochrome P-450 reductase, various cytochrome P-450 and peroxidase inhibitors, antioxidants, a...

journal_title：Archives of biochemistry and biophysics

authors： Krause RJ,Ripp SL,Sausen PJ,Overby LH,Philpot RM,Elfarra AA

更新日期：1996-09-01 00:00:00

abstract：:The cytochromes P450 are capable of oxidizing a variety of xenobiotics. Binding of a small molecule heteroactivator to a P450 can alter the coupling of substrate oxidation during P450 catalysis, but the degree to which coupling or shunting via one of the three catalytic cycle branch points is linked to the heteroactiv...

journal_title：Archives of biochemistry and biophysics

authors： Locuson CW,Gannett PM,Tracy TS

更新日期：2006-05-15 00:00:00

abstract：:In 5-aminolevulinic acid (ALA)-based photodynamic therapy (PDT), ALA taken up by tumor cells is metabolized to protoporphyrin IX (PpIX), which sensitizes photodamage leading to apoptotic or necrotic cell death. Since lipophilic PpIX originates in mitochondria, we postulated that photoperoxidation of highly unsaturated...

journal_title：Archives of biochemistry and biophysics

authors： Kriska T,Korytowski W,Girotti AW

更新日期：2005-01-15 00:00:00

abstract：:An acid phosphatase from Brassica nigra (black mustard) leaf petiole cell-suspension cultures has been purified 1633-fold to a final specific activity of 1225 (mumols orthophosphate produced/min)/mg protein and near homogeneity. The native protein was a glycosylated monomer having a molecular mass of 60 kDa and a pI o...

journal_title：Archives of biochemistry and biophysics

authors： Duff SM,Lefebvre DD,Plaxton WC

更新日期：1991-04-01 00:00:00

abstract：:Adenylate cyclase activation by GTP and octopamine as well as basal activity (in the presence of Mg2+) have been studied as a function of membrane structure in plasma membranes from brain of the dipterous Ceratitis capitata. Benzyl alcohol and lidocaine, but not phenobarbital, inhibited the three activities to the sam...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Gavilanes FG,Municio AM

更新日期：1991-05-01 00:00:00

abstract：:Four different monoclonal antibodies (MAbs) to human epidermal growth factor (hEGF) were obtained from mouse hybridoma cell lines. They were highly specific for hEGF and did not cross-react appreciably with mouse EGF. The immunoglobulin class of these MAbs was IgG1 or IgG2a. The apparent Kd values for hEGF of these MA...

journal_title：Archives of biochemistry and biophysics

authors： Yoshitake Y,Nishikawa K

更新日期：1988-06-01 00:00:00

abstract：:The thermal denaturation of clathrin-coated vesicles isolated from bovine brain tissue has been studied by differential scanning calorimetry and has been compared to basket structures reformed from isolated triskelion trimers of clathrin and to isolated triskelions. The coated vesicles and reformed baskets displayed s...

journal_title：Archives of biochemistry and biophysics

authors： Schwarz FP,Steer CJ,Kirchhoff WH

更新日期：1989-09-01 00:00:00

abstract：:Two complementary DNA (cDNA) clones (pTK-1 and -2) encoding two distinct isotypes of mouse Mg(2+)-dependent protein phosphatase beta (MPP beta-1 and -2, respectively) were isolated from a melanocyte cDNA library. Although mouse pTK-1 is orthologous to the rat cDNA (JW5) reported previously [Wenk, J., Trompeter, H.I., ...

journal_title：Archives of biochemistry and biophysics

authors： Terasawa T,Kobayashi T,Murakami T,Ohnishi M,Kato S,Tanaka O,Kondo H,Yamamoto H,Takeuchi T,Tamura S

更新日期：1993-12-01 00:00:00

abstract：:Catalases are biotechnologically relevant enzymes because of their applications in food technology, bioremediation, and biomedicine. The dismutation of hydrogen peroxide occurs in two steps; in the first one, the enzyme forms an oxidized compound I (Cpd I) and in the second one, the enzyme is reduced to the ferric sta...

journal_title：Archives of biochemistry and biophysics

authors： Zárate-Romero A,Stojanoff V,Cohen AE,Hansberg W,Rudiño-Piñera E

更新日期：2019-05-15 00:00:00

abstract：:Saccharomyces cerevisiae sterol and heme auxotrophs were used to elucidate a role for hemes in sterol esterification. Steryl ester synthase (SES) activity was stimulated on average fourfold in cells supplemented with 50 micrograms/ml delta-aminolevulinic acid (ALA). This stimulation was not dependent on ALA per se, bu...

journal_title：Archives of biochemistry and biophysics

authors： Keesler GA,Casey WM,Parks LW

更新日期：1992-08-01 00:00:00

abstract：:The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

journal_title：Archives of biochemistry and biophysics

authors： Salvador JM,Mata AM

更新日期：1998-03-15 00:00:00

abstract：:We have studied the effectiveness of polyprenyl-P-mannose and polyprenol-P-glucose as donor substrates for the dolichyl-P-mannose:Man5(GlcNAc)2-PP-dolichol mannosyltransferase and the dolichyl-P-glucose:Man9(GlcNAc)2-PP-dolichol glucosyltransferase, respectively. The polyprenol moiety differs from dolichol only in the...

journal_title：Archives of biochemistry and biophysics

authors： D'Souza-Schorey C,McLachlan KR,Krag SS,Elbein AD

更新日期：1994-02-01 00:00:00

abstract：:Calcium ion is an uncompetitive inhibitor of the inorganic pyrophosphatases of bovine heart and rat liver mitochondria with respect to substrate MgPPi at pH 8.5 and a non-competitive inhibitor of the former enzyme at pH 7.2. The concentration of Ca2+ required to decrease the maximal velocities for both enzymes at pH 8...

journal_title：Archives of biochemistry and biophysics

authors： Baykov AA,Volk SE,Unguryte A

更新日期：1989-09-01 00:00:00

abstract：:Phosphatidylethanols (PEths) are negatively charged diacyl phospholipids that are ubiquitously present in humans under the condition of alcohol intoxication. These lipids, derived in vivo from other naturally occurring phospholipids such as phosphatidylcholines (PC) via transphosphatidylation reaction as catalyzed by ...

journal_title：Archives of biochemistry and biophysics

authors： Li S,Lin H,Wang G,Huang CH

更新日期：2001-01-01 00:00:00

abstract：:The secondary structures of staphylococcal nuclease (SNase) have been assigned and semiquantitatively estimated from the deconvoluted Fourier transformed infrared (FTIR) spectrum. The changes in the secondary structures accompanying unfolding and refolding of SNase during reversible thermal denaturation up to 70 degre...

journal_title：Archives of biochemistry and biophysics

authors： Xie L,Jing GZ,Zhou JM

更新日期：1996-04-01 00:00:00

abstract：:Nanomechanical properties of living cells, as measured with atomic force microscopy (AFM), are increasingly recognized as criteria that differentiate normal and pathologically altered cells. Locally measured cell elastic properties, described by the parameter known as Young's modulus, are currently proposed as a new d...

journal_title：Archives of biochemistry and biophysics

authors： Pogoda K,Pięta E,Roman M,Piergies N,Liberda D,Wróbel TP,Janmey PA,Paluszkiewicz C,Kwiatek WM

更新日期：2021-01-15 00:00:00

abstract：:Polyphyllin I (PPI), a bioactive constituent extracted from the rhizomes of Paris polyphylla, is cytotoxic to several cancer types. This study was designed to explore whether PPI prevents non-small-cell lung cancer (NSCLC) growth and to investigate the molecular mechanism. AMP-activated protein kinase (AMPK) has been ...

journal_title：Archives of biochemistry and biophysics

authors： Wu Y,Si Y,Xiang Y,Zhou T,Liu X,Wu M,Li W,Zhang T,Xiang K,Zhang L,Zhao H,Liu Y

更新日期：2020-07-15 00:00:00

abstract：:The kinetic properties of UDP-glucuronosyltransferase were measured after brief treatment of liver microsomes at alkaline pH, followed by assay with p-nitro-phenol as aglycone, at pH 7.5. Enzyme activity increased in a graded fashion as the pH of pretreatment was increased above 8.0, with apparent maximal activation o...

journal_title：Archives of biochemistry and biophysics

authors： Dannenberg A,Wong T,Zakim D

更新日期：1990-03-01 00:00:00

abstract：:An anti-avidin mAb, viz., H12G4, is shown to release bound biotin in a dose-dependent manner from holoavidin and holostreptavidin and inhibit the binding of ligand to the two apoproteins. The release of biotin by this mAb is accompanied by quenching of ligand-induced enhanced fluorescence of the FITC-avidin conjugate....

journal_title：Archives of biochemistry and biophysics

authors： Subramanian N,Subramanian S,Karande AA,Adiga PR

更新日期：1997-08-15 00:00:00

abstract：:Leukotoxin (ltx) and isoleukotoxin (iltx) methyl esters, are metabolites of methyl linoleic acid, an essential fatty acid. They have been associated with acute respiratory distress syndrome. The observed toxicity of ltx and iltx is, in fact, due to the metabolism of the epoxides to their corresponding diols by soluble...

journal_title：Archives of biochemistry and biophysics

authors： Greene JF,Williamson KC,Newman JW,Morisseau C,Hammock BD

更新日期：2000-04-15 00:00:00

abstract：:The application of molecular dynamics simulations to study the folding and dynamics of peptides has attracted a lot of interest in the last couple of decades. Following the successful prediction of the folding of several proteins using molecular simulation, foldable peptides emerged as a favourable system mainly due t...

journal_title：Archives of biochemistry and biophysics

authors： Georgoulia PS,Glykos NM

更新日期：2019-03-30 00:00:00

abstract：:Inhibition of the catecholase and cresolase reactions of the alpha, beta, and gamma isozymes of Agaricus bisporus tyrosinase by benzoic acid was investigated at 25.0 and 8.0 degrees C at pH 5.60 in air-saturated solutions. Benzoic acid is a simple competitive inhibitor of the cresolase reaction of all three isozymes. ...

journal_title：Archives of biochemistry and biophysics

authors： Menon S,Fleck RW,Yong G,Strothkamp KG

更新日期：1990-07-01 00:00:00

abstract：:Cancer osaka thyroid (COT), a human MAP 3 K, is essential for lipopolysaccharide activation of the Erk MAPK cascade in macrophages. COT 30--467 is insoluble, whereas low levels of COT 30--397 can be expressed, but this protein is unstable. However, both COT 30--467 and COT 30--397 are expressed in a soluble and stable...

journal_title：Archives of biochemistry and biophysics

authors： Jia Y,Quinn CM,Bump NJ,Clark KM,Clabbers A,Hardman J,Gagnon A,Kamens J,Tomlinson MJ,Wishart N,Allen H

更新日期：2005-09-01 00:00:00

abstract：:This report describes the isolation, sequencing, and functional analysis of the sodA gene, encoding Mn-superoxide dismutase, from Streptococcus thermophilus AO54. The gene was found to encode a 201 amino acid polypeptide with 88 and 83% identity to SodA from Streptococcus mutans and Streptococcus agalacticae, respecti...

journal_title：Archives of biochemistry and biophysics

authors： Andrus JM,Bowen SW,Klaenhammer TR,Hassan HM

更新日期：2003-12-01 00:00:00

abstract：:Lysosomes were isolated from Chinese hamster ovary cells by fractionation of a postnuclear supernatant in consecutive density gradients. By marker enzyme analysis, the preparation was 63-fold enriched for lysosomes compared to the homogenate and contained at most trace amounts of marker activities for plasma membrane,...

journal_title：Archives of biochemistry and biophysics

authors： Madden EA,Wirt JB,Storrie B

更新日期：1987-08-15 00:00:00