A differential scanning calorimetric study of brain clathrin.

abstract：:The intrinsic fluorescence of potato tuber pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP) was used as an indicator of conformational changes due to ligand binding. Binding of the substrates and the allosteric activator fructose-2,6-bisphosphate was quantitatively compared to their respective kinetic eff...

journal_title：Archives of biochemistry and biophysics

authors： Podestá FE,Plaxton WC

更新日期：2003-06-01 00:00:00

abstract：:Rats fed an ethanol-containing diet for 4 weeks showed a 3- to 5-fold increase over isocalorically pair-fed controls with respect to cytosolic NAD(P)H-quinone oxidoreductase (NQOR) (E.C.1.6.99.2) with both menadione and dichlorophenol-indophenol as substrates. Rates of NAD(P)H-dependent p-nitrosophenol (pNSP) reductio...

journal_title：Archives of biochemistry and biophysics

authors： Hajos AK,Winston GW

更新日期：1992-06-01 00:00:00

abstract：:Several 2-halo-acyl-CoA derivatives have been synthesized to examine the interaction of these potential inhibitors of mitochondrial beta-oxidation with the purified medium chain acyl-CoA dehydrogenase from pig kidney. Racemic 2-bromo-, 2-chloro-, and 2-fluoro-octanoyl-CoA thioesters show 6.6, 33, and 3.5% of the activ...

journal_title：Archives of biochemistry and biophysics

authors： Cummings JG,Thorpe C

更新日期：1993-04-01 00:00:00

abstract：:Spinach chloroplast NAD(P)-glyceraldehyde-3-phosphate dehydrogenase (NAD(P)-GAPDH; EC, 1.2.1.13) was purified as the 600-kDa oligomer of low specific activity. Incubation of the enzyme with either a reductant or a 1,3-bisphosphoglycerate (1,3bisPGA) generating system, but most effectively with both, resulted in an inc...

journal_title：Archives of biochemistry and biophysics

authors： Baalmann E,Backhausen JE,Rak C,Vetter S,Scheibe R

更新日期：1995-12-20 00:00:00

abstract：:Previous studies with methyl ethyl ketone peroxide (MEKP), a radical generator, showed depletion of plasma vitamin E and liver glutathione (GSH) levels prior to a decrease of liver vitamin E levels. Since hepatic pools of this vitamin may serve to maintain circulating levels of vitamin E under conditions of oxidative ...

journal_title：Archives of biochemistry and biophysics

authors： Warren DL,Brown MK,Reed DJ

更新日期：1991-08-01 00:00:00

abstract：:Cytochrome P4501B1 (Cyp1b1) is expressed specifically in certain neural crest (NC) cells during embryogenesis. Mesenchymal progenitor cells that develop from NC cells are modeled here by mouse C3H10T1/2 and 3T3-L1 cells. Dexamethasone in combination with methylisobutylxanthine (DM) induces Cyp1b1 and a 6.7 kb mouse Cy...

journal_title：Archives of biochemistry and biophysics

authors： Zheng W,Tong T,Lee J,Liu X,Marcus C,Jefcoate CR

更新日期：2013-04-01 00:00:00

abstract：:The relationship between erythroid delta-aminolevulinate (ALA) synthase and hepatic ALA synthase in rat was analyzed immunochemically, using antibodies directed against rat liver ALA synthase and against chicken liver ALA synthase. Rat erythroid ALA synthase showed no cross-reactivity with anti-liver ALA synthase anti...

journal_title：Archives of biochemistry and biophysics

authors： Yamamoto M,Fujita H,Watanabe N,Hayashi N,Kikuchi G

更新日期：1986-02-15 00:00:00

abstract：:The effect of the vinca alkaloid drugs, vincristine, vinblastine, catharanthine, and vindoline, on the aging process of tubulin has been examined. It was found that addition of vincristine or vinblastine accelerated by a factor of 3-3.5 the transformation of tubulin from the 5.8 S alpha-beta-tubulin dimer to paucidisp...

journal_title：Archives of biochemistry and biophysics

authors： Prakash V,Timasheff SN

更新日期：1992-05-15 00:00:00

abstract：:Dystrophic chicken breast muscle mitochondria contain significantly less mitochondrial creatine kinase than normal breast muscle mitochondria. Breast muscle mitochondria from normal 16- to 40-day-old chickens contain approximately 80 units of mitochondrial creatine kinase per unit of succinate:INT (p-iodonitrotetrazol...

journal_title：Archives of biochemistry and biophysics

authors： Bennett VD,Hall N,DeLuca M,Suelter CH

更新日期：1985-07-01 00:00:00

abstract：:The branched-chain alpha-ketoacid dehydrogenase complex, catalyst for the rate-limiting step of branched-chain amino acid catabolism, is controlled by a highly specific protein kinase (branched-chain alpha-ketoacid dehydrogenase kinase) that associates tightly with the complex. The activity state (proportion of the en...

journal_title：Archives of biochemistry and biophysics

authors： Popov KM,Zhao Y,Shimomura Y,Jaskiewicz J,Kedishvili NY,Irwin J,Goodwin GW,Harris RA

更新日期：1995-01-10 00:00:00

abstract：:The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

journal_title：Archives of biochemistry and biophysics

authors： Iannotti AM,Rabideau DA,Dougherty JJ

更新日期：1988-07-01 00:00:00

abstract：:The relative rates of transcription of the rat liver glutathione S-transferase Ya-Yc and Yb genes were determined in purified liver nuclei isolated at different times after phenobarbital or 3-methylcholanthrene administration. The transcriptional rates of the Ya-Yc and Yb genes were elevated approximately fivefold 8 a...

journal_title：Archives of biochemistry and biophysics

authors： Ding VD,Pickett CB

更新日期：1985-08-01 00:00:00

abstract：:An in vitro assay that measures endosome fusion was used to characterize the role of guanosine triphosphate (GTP)-binding proteins in endocytosis. Guanosine 5',3-(thio)triphosphate (GTP gamma S), a nonhydrolyzable analog of GTP, stimulates the binding of cytosolic factors to the endosomal membrane (priming). GTP gamma...

journal_title：Archives of biochemistry and biophysics

authors： Lenhard JM,Colombo MI,Stahl PD

更新日期：1994-08-01 00:00:00

abstract：:Isolated mitochondria loaded with Ca(2+) and then exposed to Fe(2+) show a transient release of Ca(2+). The magnitude of this response depends on the Ca(2+) loading and the kinetics of the response depends on the concentration of added Fe(2+). We investigated the Fe(2+)-induced Ca(2+) release mechanism by measuring mi...

journal_title：Archives of biochemistry and biophysics

authors： Gogvadze V,Walter PB,Ames BN

更新日期：2002-02-15 00:00:00

abstract：:Heparin infusion in an experimental animal model of arterial injury causes a significant increase in the proteoglycan (PG) component of the extracellular matrix in the injured arteries (Snow, A. D., Bolender, R. P., Wight, T. N., and Clowes, A. W. (1990) Am. J. Pathol. 137, 313-330). The mechanisms responsible for thi...

journal_title：Archives of biochemistry and biophysics

authors： Potter-Perigo S,Wight TN

更新日期：1996-12-01 00:00:00

abstract：:Previous experiments performed in recombinant systems have suggested that protein-protein interactions occur between the UGTs and may play a significant role in modulating enzyme activity. However, evidence of UGT protein-protein interactions either in vivo or in more physiologically relevant in vitro systems has yet ...

journal_title：Archives of biochemistry and biophysics

authors： Konopnicki CM,Dickmann LJ,Tracy JM,Tukey RH,Wienkers LC,Foti RS

更新日期：2013-07-15 00:00:00

abstract：:Enzymes subjected to freeze-thawing are known to be protected by polymers that are preferentially excluded from the hydrated surface of proteins [reviewed in Carpenter et al. (1994) ACS Symp. Ser. 567, 134-147]. Preferentially excluded solutes are also known to stabilize quaternary structure, which enhances the thermo...

journal_title：Archives of biochemistry and biophysics

authors： Anchordoquy TJ,Carpenter JF

更新日期：1996-08-15 00:00:00

abstract：:The partial degradation of proteoglycan aggregate by human leucocyte elastase yielded products that banded with Mr 190,000, 140,000, 88,000, and 71,000 when analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis. Analysis of these bands revealed that the 190,000- and 140,000-Da bands contained chondro...

journal_title：Archives of biochemistry and biophysics

authors： Mok MT,Ilic MZ,Handley CJ,Robinson HC

更新日期：1992-02-01 00:00:00

abstract：:Myeloperoxidase (MPO), eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase are heme-containing oxidoreductases (EC 1.7.1.11), which bind ligands and/or undergo a series of redox reactions. Though sharing functional and structural homology, reflecting their phylogenetic origin, differences are observed regar...

journal_title：Archives of biochemistry and biophysics

authors： Furtmüller PG,Zederbauer M,Jantschko W,Helm J,Bogner M,Jakopitsch C,Obinger C

更新日期：2006-01-15 00:00:00

abstract：:Reactive oxygen species like hydrogen peroxide (H2O2) have been shown to serve as messengers in the induction of NF-kappa B and, hence, in the activation and replication of human immunodeficiency virus type 1 (HIV-1) in human cells. Several antioxidant compounds and iron chelators have been shown to interfere with bot...

journal_title：Archives of biochemistry and biophysics

authors： Sappey C,Boelaert JR,Legrand-Poels S,Grady RW,Piette J

更新日期：1995-08-01 00:00:00

abstract：:Inhibition of the catecholase and cresolase reactions of the alpha, beta, and gamma isozymes of Agaricus bisporus tyrosinase by benzoic acid was investigated at 25.0 and 8.0 degrees C at pH 5.60 in air-saturated solutions. Benzoic acid is a simple competitive inhibitor of the cresolase reaction of all three isozymes. ...

journal_title：Archives of biochemistry and biophysics

authors： Menon S,Fleck RW,Yong G,Strothkamp KG

更新日期：1990-07-01 00:00:00

abstract：:Bovine collateral ligament synthesized a 35S-labeled large proteoglycan species which eluted with a Kav of approximately 0.27 on Sepharose CL-2B and contained only chondroitin sulfate chains with a molecular mass of approximately 32 kDa. Fluorography of the 35S-labeled core proteins derived from the large ligament pro...

journal_title：Archives of biochemistry and biophysics

authors： Campbell MA,Tester AM,Handley CJ,Checkley GJ,Chow GL,Cant AE,Winter AD,Cain WE

更新日期：1996-05-15 00:00:00

abstract：:Thymidylate synthase (TS) is an essential enzyme that must be expressed in all proliferating cells. The mouse TS promoter lacks a TATA box and an initiator element and initiates transcription over a broad region in cultured fibroblasts. The goal of this study was to determine if expression of the TS gene in a variety ...

journal_title：Archives of biochemistry and biophysics

authors： Lee Y,Shen G,Johnson LF

更新日期：1999-12-15 00:00:00

abstract：:The effects of orally administered secondary autoxidation products of linoleic acid in rat liver were investigated. Their administration led to two toxic effects on hepatic carbohydrate metabolism, as compared to the administration of saline or linoleic acid used as controls. One effect was depletion of glucose 6-phos...

journal_title：Archives of biochemistry and biophysics

authors： Ashida H,Kanazawa K,Minamoto S,Danno G,Natake M

更新日期：1987-11-15 00:00:00

abstract：:The effects of insulin on phospholipid metabolism and generation of diacylglycerol (DAG) and on activation of protein kinase C in rat hepatocytes were compared to those of vasopressin and angiotension II. Insulin provoked increases in [3H]glycerol labeling of phosphatidic acid (PA), diacylglycerol (DAG), and other gly...

journal_title：Archives of biochemistry and biophysics

authors： Cooper DR,Hernandez H,Kuo JY,Farese RV

更新日期：1990-02-01 00:00:00

abstract：:We investigated the role of free radicals in hemoglobin (Hb) oxidation and denaturation. To generate free radicals, we used two azocompounds, the hydrophilic 2,2'-azobis(2-amidinopropane hydrochloride and the hydrophobic 2,2'-azobis(2,4-dimethylvaleronitrile) and a drug of the quinone family, phenazine methosulfate. T...

journal_title：Archives of biochemistry and biophysics

authors： Minetti M,Mallozzi C,Scorza G,Scott MD,Kuypers FA,Lubin BH

更新日期：1993-04-01 00:00:00

abstract：:Low-temperature plasma is useful for the care of wounded skin. It accelerates wound healing. However, the mechanism of this effect has not been fully elucidated yet. Galectin-1 is reported to accelerate wound healing via the Smad signaling pathway. In the present study to clarify whether or not galectins were expresse...

journal_title：Archives of biochemistry and biophysics

authors： Akimoto Y,Ikehara S,Yamaguchi T,Kim J,Kawakami H,Shimizu N,Hori M,Sakakita H,Ikehara Y

更新日期：2016-09-01 00:00:00

abstract：:Using the polymerase chain reaction (PCR) a 0.8-kb product was amplified from cDNA made from the midgut and Malpighian tubules of fifth instar larvae of Helicoverpa virescens. This 0.8-kb PCR product was then used to isolate a clone of the B subunit of the V-type ATPase from a cDNA library made from the same tissues. ...

journal_title：Archives of biochemistry and biophysics

authors： Gill SS,Ross LS

更新日期：1991-11-15 00:00:00

abstract：:Properties of phosphatidylinositol kinase activities in rabbit erythrocyte membranes were studied by measuring 32P incorporation into di- and triphosphoinositide from Mg-[gamma-32P]ATP. The Km's for 32P incorporation into di- and triphosphoinositide were 110 and 48 microM ATP, respectively. The optimal temperature for...

journal_title：Archives of biochemistry and biophysics

authors： Quist EE,Barker RC

更新日期：1983-04-01 00:00:00

abstract：:Sepsis is a life-threatening clinical syndrome defined as a deregulated host response to infection associated with organ dysfunction. Mechanisms underlying the pathophysiology of septic liver dysfunction are incompletely understood. Among others, the iron containing tetrapyrrole heme inflicts hepatic damage when relea...

journal_title：Archives of biochemistry and biophysics

authors： Englert FA,Seidel RA,Galler K,Gouveia Z,Soares MP,Neugebauer U,Clemens MG,Sponholz C,Heinemann SH,Pohnert G,Bauer M,Weis S

更新日期：2019-09-15 00:00:00