An immunochemical study of delta-aminolevulinate synthase and delta-aminolevulinate dehydratase in liver and erythroid cells of rat.

abstract：:PCNA is well known as a component of DNA replication system and plays important roles in multiple cellular pathways in addition to replication and repair. In this work we have demonstrated the physical and functional interaction between tobacco PCNA and mungbean ddNTP-sensitive DNA polymerase which shares many physico...

journal_title：Archives of biochemistry and biophysics

authors： Roy S,Roy Choudhury S,Mukherjee SK,Sengupta DN

更新日期：2007-12-01 00:00:00

abstract：:Site-mutations were introduced into putative cation binding site 1 of the H,K-ATPase at glu-797, thr-825, and glu-938. The side chain oxygen of each was not essential but the mutations produced different activation and inhibition kinetics. Site mutations thr-825 (ala, leu) and glu-938 (ala, gln) modestly decreased the...

journal_title：Archives of biochemistry and biophysics

authors： Rulli SJ,Louneva NM,Skripnikova EV,Rabon EC

更新日期：2001-03-01 00:00:00

abstract：:Neutrophils and monocytes produce the highly cytotoxic hypochlorous acid (HOCl) via the myeloperoxidase (MPO)-catalyzed reaction of H2O2 with Cl-. We have investigated the reaction of reagent and MPO-derived HOCl with cholesterol in a purified liposome system, as well as progressively more complex biological systems. ...

journal_title：Archives of biochemistry and biophysics

authors： Carr AC,van den Berg JJ,Winterbourn CC

更新日期：1996-08-01 00:00:00

abstract：:Salicylhydroxamic acid reductase, which catalyzes the reduction of salicylhydroxamic acid to salicylamide, was purified from rat liver cytosol. The purification procedure consisted of fractionation with ammonium sulfate, chromatography with Phenyl-Toyopearl 650, DEAE-cellulose, hydroxyapatite, and Sephadex G-200, and ...

journal_title：Archives of biochemistry and biophysics

authors： Katsura H,Kitamura S,Tatsumi K

更新日期：1993-05-01 00:00:00

abstract：:Using specific testosterone 16 alpha-hydroxylase activity as the basis for selection of fractions during purification, the cytochrome P-450 ("I"-P-450(16)alpha) has been isolated from livers of phenobarbital-treated 129/J female mice [K. Devore, N. Harada, and M. Negishi (1985) Biochemistry, 24, 5632-5637]. An antibod...

journal_title：Archives of biochemistry and biophysics

authors： Noshiro M,Serabjit-Singh CJ,Bend JR,Negishi M

更新日期：1986-02-01 00:00:00

abstract：:7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) is a specific covalent inhibitor of P-glycoprotein ATPase activity (M. K. Al-Shawi, and A. E. Senior, 1993, J. Biol. Chem. 268, 4197-4206). Complete inhibition occurs at a reaction stoichiometry of 1 mol NBD/mol P-glycoprotein, and the reagent has proved valuable in und...

journal_title：Archives of biochemistry and biophysics

authors： Senior AE,Gros P,Urbatsch IL

更新日期：1998-09-01 00:00:00

abstract：:Oxidation of [2,3-14C]succinate carbons in the mitochondrial Krebs cycle was used as a probe to investigate the effects of insulin, epinephrine, glucagon, and 2,4-dinitrophenol (2,4-DNP) on isolated rat hepatocytes. Epinephrine, glucagon, and 2,4-DNP had a far greater stimulatory effect on 14CO2 formation from [2,3-14...

journal_title：Archives of biochemistry and biophysics

authors： Mohan C,Memon RA,Bessman SP

更新日期：1991-05-15 00:00:00

abstract：:The motility of actin filaments interacting with heavy meromyosin molecules was directly observed on indium tin oxide-coated glass (ITO-glass), over which a surface current flowed. Because the increase in surface current applied to ITO-glass increases the temperature, we focused on the temperature-dependence of the sl...

journal_title：Archives of biochemistry and biophysics

authors： Wada R,Sato D,Nakamura T,Hatori K

更新日期：2015-11-15 00:00:00

abstract：:NADH:cytochrome b5 oxidoreductase catalyzes the transfer of reducing equivalents from the physiological electron donor, NADH, to two molecules of cytochrome b5. Utilizing a heterologous expression system for the soluble, catalytic domain of the rat microsomal enzyme, we have produced two mutants, corresponding to E255...

journal_title：Archives of biochemistry and biophysics

authors： Davis CA,Barber MJ

更新日期：2004-05-15 00:00:00

abstract：:Mitochondrial permeability transition (MPT) is a Ca(2+)-dependent, cyclosporine A-sensitive, non-selective inner membrane permeabilization induced by a wide range of agents or conditions, which has often been associated with necrotic or apoptotic cell death. When mitochondria isolated from livers of rats treated with ...

journal_title：Archives of biochemistry and biophysics

authors： Andreu GL,Delgado R,Velho JA,Curti C,Vercesi AE

更新日期：2005-07-15 00:00:00

abstract：:Calcium ion is an uncompetitive inhibitor of the inorganic pyrophosphatases of bovine heart and rat liver mitochondria with respect to substrate MgPPi at pH 8.5 and a non-competitive inhibitor of the former enzyme at pH 7.2. The concentration of Ca2+ required to decrease the maximal velocities for both enzymes at pH 8...

journal_title：Archives of biochemistry and biophysics

authors： Baykov AA,Volk SE,Unguryte A

更新日期：1989-09-01 00:00:00

abstract：:Signal transducer and activator of transcription 3 (STAT3) is expressed aberrantly in multiple tumors, including gastric cancer (GC). STAT3 overexpression and excessive activation have been confirmed to play vital roles in tumorigenesis. Cucurbitacin B (CuB) is a natural product with potent anti-cancer activities in s...

journal_title：Archives of biochemistry and biophysics

authors： Xu J,Chen Y,Yang R,Zhou T,Ke W,Si Y,Yang S,Zhang T,Liu X,Zhang L,Xiang K,Guo Y,Liu Y

更新日期：2020-05-15 00:00:00

abstract：:The solution structure of native and systematically modified ovine submaxillary mucin (OSM) has been probed by proton NMR spectroscopic methods. Most of the resonances in the spectra have been tentatively assigned to the peptide and O-linked disaccharide, alpha-N-acetylneuraminic acid 2----6 alpha-N-acetylgalactosamin...

journal_title：Archives of biochemistry and biophysics

authors： Gerken TA

更新日期：1986-06-01 00:00:00

abstract：:The reaction of phospholipid hydroperoxide glutathione peroxidase (PHGPx) and Ebselen with phospholipid and cholesterylester hydroperoxides associated with HDLox and LDLox was investigated using specific HPLC assays for the hydroperoxides of phosphatidylcholine (PCOOH) and cholesteryllinolate (Ch18:2-OOH) and for chol...

journal_title：Archives of biochemistry and biophysics

authors： Sattler W,Maiorino M,Stocker R

更新日期：1994-03-01 00:00:00

abstract：:Advancements in peptide fusion technologies to maximize the protein production has taken a big leap to fulfill the demands of post-genomics era targeting elucidation of structure/function of the proteome and its therapeutic applications, by over-expression in heterologous expression systems. Despite being most preferr...

journal_title：Archives of biochemistry and biophysics

authors： Yadav DK,Yadav N,Yadav S,Haque S,Tuteja N

更新日期：2016-12-15 00:00:00

abstract：:Thyroid peroxidase (TPO) was purified from human thyroid tissue, obtained at surgery from patients with Graves' disease, by a procedure similar to one that we had previously used for the purification of porcine TPO. The membrane-bound enzyme was solubilized by treatment of the thyroid particulate fraction with trypsin...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris ML,Yokoyama N,Slaughter C

更新日期：1990-05-01 00:00:00

abstract：:Conversion of geranyl pyrophosphate to cyclic monoterpenes is considered to involve the preliminary isomerization of this acyclic precursor to enzyme-bound linalyl pyrophosphate and the cyclization of this tertiary intermediate. 2-Fluorogeranyl pyrophosphate and 2-fluorolinalyl pyrophosphate are effective competitive ...

journal_title：Archives of biochemistry and biophysics

authors： Croteau R

更新日期：1986-12-01 00:00:00

abstract：:Despite extensive investigations on the acid-unfolded and acid/salt-induced molten globule(-like) states of cytochrome c using variety of techniques, structural features of the acid-unfolded state in terms of residual secondary structures and the structural transition between the acid-unfolded and acid/salt-refolded s...

journal_title：Archives of biochemistry and biophysics

authors： Dong A,Lam T

更新日期：2005-04-01 00:00:00

abstract：:The mitochondrial apoptosis pathway has been neatly ordered. Mitochondrial apoptosis is governed by Bcl-2 family proteins, and their respective contributions determine the release of cytochrome c. It is clear that, among the Bcl-2 family, BH3-only proteins are the triggers: activation of BH3-only proteins by apoptotic...

journal_title：Archives of biochemistry and biophysics

authors： Häcker G,Weber A

更新日期：2007-06-15 00:00:00

abstract：:The effect of modulating AP-1 activity on the levels of induced erythroid and megakaryocytic differentiation of the erythroleukemia cell line K562 was examined. Cells were stably transfected with expression vectors encoding either a tetracycline-responsive dominant negative c-Jun (JunDN) or a hybrid Raf protein induci...

journal_title：Archives of biochemistry and biophysics

authors： Rosson D,O'Brien TG

更新日期：1998-04-15 00:00:00

abstract：:The vesicle-to-micelle structural transitions that occurred in the interaction of sodium dodecyl sulfate with phosphatidylcholine vesicles were studied at the equilibrium by means of dynamic light scattering (at different scattering angles) and freeze-fracture electron microscopy techniques. The incorporation of surfa...

journal_title：Archives of biochemistry and biophysics

authors： López O,Cócera M,Wehrli E,Parra JL,de la Maza A

更新日期：1999-07-15 00:00:00

abstract：:A green manganese desferrioxamine complex is rapidly formed at room temperature upon stirring freshly precipitated manganese dioxide in a solution of the ligand. Spectral studies and low-temperature ESR indicate that this compound, which has been previously described as a manganese(III) complex, is better characterize...

journal_title：Archives of biochemistry and biophysics

authors： Rush JD,Maskos Z,Koppenol WH

更新日期：1991-08-15 00:00:00

abstract：:A detailed knowledge about the substrate specificities of the heparin lyases is necessary when using these enzymes as tools for elucidating the sequence of heparin and heparan sulfate. The substrate specificity of heparin lyases I, II, and III have been profiled with structurally defined, heparin-derived oligosacchari...

journal_title：Archives of biochemistry and biophysics

authors： Desai UR,Wang HM,Linhardt RJ

更新日期：1993-11-01 00:00:00

abstract：:Glucosidase I, the enzyme catalyzing the first step of N-linked oligosaccharide processing, has been purified from calf liver crude membranes [H. Hettkamp, G. Legler, and E. Bause, (1984) Eur. J. Biochem. 142, 85-90]. Binding experiments with concanavalin A-Sepharose suggest that glucosidase I is a glycoprotein with h...

journal_title：Archives of biochemistry and biophysics

authors： Schweden J,Borgmann C,Legler G,Bause E

更新日期：1986-07-01 00:00:00

abstract：:The stability of glutathione peroxidase was assessed in vitro via oxidative inactivation by peroxides and a peroxidizing fatty acid and by renaturation and proteolysis. The stability of glutathione peroxidase to methyl ethyl ketone peroxide, H2O2, linoleic acid hydroperoxide, and peroxidizing methyl linolenate was com...

journal_title：Archives of biochemistry and biophysics

authors： Condell RA,Tappel AL

更新日期：1983-06-01 00:00:00

abstract：:Systemic exposure to gram-negative bacterial substances such as lipopolysaccharide (LPS, or endotoxin) induces an uncontrolled, massive inflammatory reaction which culminates in multiple system organ failure and death. Septic shock often does not respond to corticosteroids; however, certain low-molecular-weight antiox...

journal_title：Archives of biochemistry and biophysics

authors： Stewart CA,Hyam K,Wallis G,Sang H,Robinson KA,Floyd RA,Kotake Y,Hensley K

更新日期：1999-05-01 00:00:00

abstract：:Cytochrome P4502E (P4502E), the major ethanol-inducible P450 metabolizes ethanol to acetaldehyde and bioactivates procarcinogens to ultimate carcinogens. Metabolism of ethanol to acetaldehyde in the brain could be deleterious since it can react with cytoskeletal proteins, forming adducts. In the present study, rats we...

journal_title：Archives of biochemistry and biophysics

authors： Upadhya SC,Tirumalai PS,Boyd MR,Mori T,Ravindranath V

更新日期：2000-01-01 00:00:00

abstract：:Reperfusion of ischemic myocardial tissue results in an increase in mitochondrial free radical production and declines in respiratory activity. The effects of ischemia and reperfusion on the activities of Krebs cycle enzymes, as well as enzymes involved in electron transport, were evaluated to provide insight into whe...

journal_title：Archives of biochemistry and biophysics

authors： Sadek HA,Humphries KM,Szweda PA,Szweda LI

更新日期：2002-10-15 00:00:00

abstract：:Guanidine hydrochloride-induced denaturation and thermal denaturation of three kinds of tryptophan synthase alpha subunit have been compared by circular dichroism measurements. The three alpha subunits are from Escherichia coli, Salmonella typhimurium, and an interspecies hybrid in which the C-terminal domain comes fr...

journal_title：Archives of biochemistry and biophysics

authors： Yutani K,Sato T,Ogasahara K,Miles EW

更新日期：1984-03-01 00:00:00

abstract：:The radioactive, photoactivatable labeling probe [beta-32P]5-azidouridine 5'-diphosphoglucose has recently been shown to label a 62-kDa protein in crude homogenates and in partially purified enzyme preparations without photoactivation. Here, we report that a portion of this radioactivity is due to labeling of phosphog...

journal_title：Archives of biochemistry and biophysics

authors： Marchase RB,Richardson KL,Srisomsap C,Drake RR,Haley BE

更新日期：1990-07-01 00:00:00