Abstract:
:PCNA is well known as a component of DNA replication system and plays important roles in multiple cellular pathways in addition to replication and repair. In this work we have demonstrated the physical and functional interaction between tobacco PCNA and mungbean ddNTP-sensitive DNA polymerase which shares many physicochemical properties with family X-DNA polymerases except with the moderately processive mode of nucleotide incorporation. We have shown here that recombinant PCNA binds directly to mungbean DNA polymerase as revealed in affinity chromatography, pull-down and co-immunoprecipitation approaches. In vitro DNA polymerase activity assay and processivity analyses indicated recombinant PCNA specifically stimulates both activity and processivity of mungbean DNA polymerase. These observations lead to interesting speculation about the functional significance of the ddNTP-sensitive enzyme in replication event in higher plants since the enzyme has been shown to be active and expressed at an elevated level during the endoreduplication stages in developing mungbean seeds.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Roy S,Roy Choudhury S,Mukherjee SK,Sengupta DNdoi
10.1016/j.abb.2007.09.009subject
Has Abstractpub_date
2007-12-01 00:00:00pages
22-31issue
1eissn
0003-9861issn
1096-0384pii
S0003-9861(07)00466-3journal_volume
468pub_type
杂志文章abstract::Phenylalanine ammonia-lyase forms trans-cinnamate from L-phenylalanine, and thus stands at a gateway to secondary metabolism in higher plants. L-alpha-Amino-oxy-beta-phenylpropanoic acid (L-AOPP), a very effective competitive inhibitor of this enzyme, is most probably a transition-state analog for the elimination reac...
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pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:1994-08-15 00:00:00
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pub_type: 杂志文章
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