Differential effects of insulin, epinephrine, and glucagon on rat hepatocyte mitochondrial activity.

abstract：:Complete structures are described for three urinary oligodextrins from one patient with type II and one patient with type III glycogen storage disease. GLC-MS, direct probe MS, and 1H NMR demonstrate two heptasaccharides and one hexasaccharide containing only alpha 1-4 and alpha 1-6 linkages. The observation that all ...

journal_title：Archives of biochemistry and biophysics

authors： Kumlien J,Grönberg G,Nilsson B,Månsson O,Zopf D,Lundblad A

更新日期：1989-03-01 00:00:00

abstract：:1-O-Sinapoylglucose:choline sinapoyltransferase ("sinapine synthase") has been purified from immature seeds of Brassica napus by sequential hydroxylapatite absorption, ion-exchange chromatography, and gel filtration. The purified enzyme has an apparent molecular weight of 65 kDa on gel filtration and a subunit structu...

journal_title：Archives of biochemistry and biophysics

authors： Vogt T,Aebershold R,Ellis B

更新日期：1993-02-01 00:00:00

abstract：:Conversion of geranyl pyrophosphate to cyclic monoterpenes is considered to involve the preliminary isomerization of this acyclic precursor to enzyme-bound linalyl pyrophosphate and the cyclization of this tertiary intermediate. 2-Fluorogeranyl pyrophosphate and 2-fluorolinalyl pyrophosphate are effective competitive ...

journal_title：Archives of biochemistry and biophysics

authors： Croteau R

更新日期：1986-12-01 00:00:00

abstract：:A series of 18 phenacyl bromide and iodoacetamide analogues have been synthesized and used to alkylate Met-398 situated in the S'1 binding site of carboxypeptidase Y. The course of the reactions was monitored by measurements of the peptidase and esterase activities. All except four of the reagents reacted selectively,...

journal_title：Archives of biochemistry and biophysics

authors： Kanstrup A,Breddam K,Buchardt O

更新日期：1993-08-01 00:00:00

abstract：:We have characterized a Brassica rapa mutant (WR586) that has low levels of polyunsaturated fatty acids (D.L. Auld et al., 1992, Crop Sci. 32, 657-662). The mutant lacked oleoyl-phosphatidylcholine desaturase (ODS) activity when assayed in 6-day-old seedlings. To further characterize the mutant, the leaf fatty acid co...

journal_title：Archives of biochemistry and biophysics

authors： Lee MS,Guerra DJ

更新日期：1994-11-15 00:00:00

abstract：:Actinomycin D (act-D) anchors itself into DNA-base pairs by intercalation and thereby inhibits mRNA synthesis. It has been well established that act-D elicits apoptosis in various cell types involving endothelial cells. However, the regulatory mechanisms of actinomycin D-induced apoptotic cell death still remain uncle...

journal_title：Archives of biochemistry and biophysics

authors： Shim D,Kang HY,Jeon BW,Kang SS,Chang SI,Kim HY

更新日期：2004-05-15 00:00:00

abstract：:Protein synthesis and lipid peroxidation were evaluated in rat liver slices incubated in the presence of oxidants and protein synthesis inhibitors. Protein synthesis by rat liver slices was evaluated by [3H]leucine incorporation into the trichloroacetic acid (TCA)-insoluble material, and lipid peroxidation was evaluat...

journal_title：Archives of biochemistry and biophysics

authors： Fraga CG,Zamora R,Tappel AL

更新日期：1989-04-01 00:00:00

abstract：:To establish a cell line expressing enhanced levels of beta-amyloid precursor protein (beta-APP), we constructed plasmid DNAs expressing beta-APP-751 mRNA and transfected them into COS-1 cells. Using a modified version of the reverse transcriptase polymerase chain reaction which is RNA sensitive to study the beta-APP ...

journal_title：Archives of biochemistry and biophysics

authors： Ramakrishna N,Smedman M,Gillam B

更新日期：1996-02-15 00:00:00

abstract：:Accumulating evidence suggests that oxidative stress resulting in reactive oxygen species generation plays a pivotal role in neurodegenerative diseases, supporting the realization of the use of radical scavengers, metal chelator agents, such as the natural polyphenols for therapy. In this study, we have focused on spe...

journal_title：Archives of biochemistry and biophysics

authors： Weinreb O,Amit T,Youdim MB

更新日期：2008-08-15 00:00:00

abstract：:Cell extracts of Methanobacterium thermoautotrophicum possess a methenyl-tetrahydromethanopterin (methenyl-H4MPT) cyclohydrolase. The enzyme catalyzes the hydrolysis of methenyl-H4MPT to formyltetrahydromethanopterin (formyl-H4MPT). The reaction is reversible and both the rate and extent of the reaction depend on the ...

journal_title：Archives of biochemistry and biophysics

authors： Donnelly MI,Escalante-Semerena JC,Rinehart KL Jr,Wolfe RS

更新日期：1985-11-01 00:00:00

abstract：:The metabolic fates of NG,NG-and NG,N'G-dimethylarginines in rats were investigated isotopically and novel metabolites, alpha-keto-delta-(N,N-dimethylguanidino)-and alpha-keto-delta-(N,N'-dimethylguanidino)valeric acids and gamma-(N,N-dimethylguanidino)-and gamma-(N,N'-dimethylguanidino)butyric acids were identified. ...

journal_title：Archives of biochemistry and biophysics

authors： Ogawa T,Kimoto M,Watanabe H,Sasaoka K

更新日期：1987-02-01 00:00:00

abstract：:In trout and mammals, the major extrahepatic expression site for CYP3A forms is in the intestine. A cDNA encoding a new CYP3A subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (RT-PCR), followed by rapid amplification of cDNA ends (RACE)-PCR. In a set o...

journal_title：Archives of biochemistry and biophysics

authors： Lee SJ,Buhler DR

更新日期：2003-04-01 00:00:00

abstract：:To evaluate the conformation of reduced HEL, the monoclonal antibodies HyC1 and HyC2, which recognize different conformational epitopes on native hen egg lysozyme (HEL), were used, and the kinetics of their interactions with native HEL, S-1,2-dicarboxyethylated HEL (DCE-HEL), and carboxymethylated Cys6 and Cys127 HEL ...

journal_title：Archives of biochemistry and biophysics

authors： Oda M,Kitai A,Murakami A,Nishimura M,Ohkuri T,Abe Y,Ueda T,Nakamura H,Azuma T

更新日期：2010-02-15 00:00:00

abstract：:The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces a novel poly(ADP-ribose) polymerase (TiPARP). In this study, the signaling pathway of the induction was analyzed. Induction of TiPARP by TCDD occurs in both hepa1c1c7 cells and C57 mouse liver. Induction is concentration and time dependen...

journal_title：Archives of biochemistry and biophysics

authors： Ma Q

更新日期：2002-08-15 00:00:00

abstract：:The Ah receptor from rat liver has been purified, using a specific oligonucleotide affinity column, in order to characterize the components of the receptor and to investigate features that modulate its DNA-binding activity. The purified DNA-binding form of rat Ah receptor contains three major components, with estimate...

journal_title：Archives of biochemistry and biophysics

authors： Ireland RC,Li SY,Dougherty JJ

更新日期：1995-06-01 00:00:00

abstract：:Three strains of Escherichia coli differing only in the catalase locus mutated by transposon Tn10 were constructed. These strains produced only catalase HPI (katE::Tn10 and katF::Tn10 strains) or catalase HPII (katG::Tn10). HPI levels increased gradually about twofold during logarithmic growth but did not increase dur...

journal_title：Archives of biochemistry and biophysics

authors： Loewen PC,Switala J,Triggs-Raine BL

更新日期：1985-11-15 00:00:00

abstract：:The superoxide dismutase from Mycobacterium tuberculosis is the only Cu-containing superoxide dismutase that lacks zinc in the active site. To explore the structural properties of this unusual enzyme, we have investigated its stability by differential scanning calorimetry. We have found that the holo-enzyme is signifi...

journal_title：Archives of biochemistry and biophysics

authors： D'Orazio M,Cervoni L,Giartosio A,Rotilio G,Battistoni A

更新日期：2009-06-15 00:00:00

abstract：:Four different monoclonal antibodies (MAbs) to human epidermal growth factor (hEGF) were obtained from mouse hybridoma cell lines. They were highly specific for hEGF and did not cross-react appreciably with mouse EGF. The immunoglobulin class of these MAbs was IgG1 or IgG2a. The apparent Kd values for hEGF of these MA...

journal_title：Archives of biochemistry and biophysics

authors： Yoshitake Y,Nishikawa K

更新日期：1988-06-01 00:00:00

abstract：:A recent note from our laboratory reported that L-band (1.2 GHz) electron paramagnetic resonance spectroscopy can be utilized in detecting the formation of Cr(V) intermediates from chromate-treated whole mice. Since Cr(V) is thought to be one of the key species in the mechanism of chromate's toxicity, we carried out a...

journal_title：Archives of biochemistry and biophysics

authors： Liu KJ,Shi X,Jiang JJ,Goda F,Dalal N,Swartz HM

更新日期：1995-10-20 00:00:00

abstract：:Some properties and subcellular localization of adenosine diphosphatase (ADPase) activity from rat heart have been investigated. The pH optimum was 7.4, maximal activity was found with 5 mM MgCl2, and the apparent Km was 20 microM. ADPase activity was strongly inhibited by NaF and AppNHp, and to a lesser extent by AMP...

journal_title：Archives of biochemistry and biophysics

authors： de Vente J,Velema J,Zaagsma J

更新日期：1984-08-15 00:00:00

abstract：:Two different fractions were present in crystalline bovine liver catalase, and could be resolved using dye-ligand affinity chromatography with Red-A Matrex gel containing Procion HE 3B. The major part (alpha) was not adsorbed on this gel. The second fraction (beta) was firmly adsorbed to the gel, and could be eluted e...

journal_title：Archives of biochemistry and biophysics

authors： Jouve HM,Pelmont J,Gaillard J

更新日期：1986-07-01 00:00:00

abstract：:The sensitivity of animals to endotoxin differs significantly between species. Thus, factors that determine the susceptibility to endotoxin may play important roles in the pathogenesis of septic shock. In order to determine the mechanism responsible for susceptibility to endotoxin, the effect of lipopolysaccharide (LP...

journal_title：Archives of biochemistry and biophysics

authors： Kida T,Kuroki T,Kobayashi K,Inoue M

更新日期：1994-07-01 00:00:00

abstract：:We have previously identified and characterized GSHPx-GI, which is a cellular selenium-dependent glutathione peroxidase (GSHPx) distinct from the classic GSHPx-1 and phospholipid hydroperoxide glutathione peroxidase (PHGPX). We have determined the level of GSHPx-GI mRNA expression in the rat gastrointestinal tract fro...

journal_title：Archives of biochemistry and biophysics

authors： Chu FF,Esworthy RS

更新日期：1995-11-10 00:00:00

abstract：:The short term response of the L-6 cell line of rat skeletal myoblasts to elevated extracellular iron concentrations was studied. It was found in all cases that iron as the nitrilotriacetate (NTA) chelate was effective at donating iron to the cells and at stimulating ferritin synthesis. After 48 h in 50 microM ferric ...

journal_title：Archives of biochemistry and biophysics

authors： Rittling SR,Woodworth RC

更新日期：1983-08-01 00:00:00

abstract：:Lysosomes were isolated from Chinese hamster ovary cells by fractionation of a postnuclear supernatant in consecutive density gradients. By marker enzyme analysis, the preparation was 63-fold enriched for lysosomes compared to the homogenate and contained at most trace amounts of marker activities for plasma membrane,...

journal_title：Archives of biochemistry and biophysics

authors： Madden EA,Wirt JB,Storrie B

更新日期：1987-08-15 00:00:00

abstract：:The objective of this study was to investigate the antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A (ConA) on human melanoma A375 cells. We firstly simulated the three-dimensional structure of ConA. Subsequently, we found that ConA possessed remarkable antiproliferative effect on A375 cell...

journal_title：Archives of biochemistry and biophysics

authors： Liu B,Li CY,Bian HJ,Min MW,Chen LF,Bao JK

更新日期：2009-02-01 00:00:00

abstract：:The photoreduction of soluble plastocyanin in a homologous reconstituted system requires a resolved Photosystem II preparation and the cytochrome b6-f complex from spinach chloroplasts. Oxygen evolution is linked quantitatively to plastocyanin reduction in the reconstituted system, indicating that water is the electro...

journal_title：Archives of biochemistry and biophysics

authors： Lam E,Malkin R

更新日期：1983-07-15 00:00:00

abstract：:Purple Cu(A) centers are a class of binuclear, mixed-valence copper complexes found in cytochrome c oxidase and nitrous oxide reductase. An engineered Cu(A) protein was formed by replacing a portion of the amino acid sequence that contains three of the ligands to the native type I copper center of Pseudomonas aerugino...

journal_title：Archives of biochemistry and biophysics

authors： Sun D,Wang X,Davidson VL

更新日期：2002-08-01 00:00:00

abstract：:Halogenated and polycyclic aromatic hydrocarbons, exogenous ligands of the aryl hydrocarbon receptor (AhR), cause various toxicological effects through the transformation of the AhR. In this study, we investigated the antagonistic effects of indigoids on the transformation in addition to their agonistic ones. In a cel...

journal_title：Archives of biochemistry and biophysics

authors： Nishiumi S,Yamamoto N,Kodoi R,Fukuda I,Yoshida K,Ashida H

更新日期：2008-02-15 00:00:00

abstract：:Mucus hypersecretion is a common feature in chronic airway diseases, and serine proteases play a critical role in this process. However, the mechanisms by which serine proteases induce mucin5AC hypersecretion have not been fully explored. In this study, we characterized human airway trypsin-like protease (HAT), a seri...

journal_title：Archives of biochemistry and biophysics

authors： Liu C,Li Q,Zhou X,Kolosov VP,Perelman JM

更新日期：2013-07-15 00:00:00