Ferritin synthesis in rat L-6 cells: response to iron challenge.

abstract：:The rotating frame nuclear magnetic resonance relaxation rate R(1rho) in the blood and cell lysate was studied at 4.7T to provide reference values for in vivo modeling and to address the mechanisms contributing to net relaxation. A strong dependence on oxygenation, hematocrit, and spin lock field strength B(1) (0.2-1....

journal_title：Archives of biochemistry and biophysics

authors： Johanna Silvennoinen M,Kettunen MI,Clingman CS,Kauppinen RA

更新日期：2002-09-01 00:00:00

abstract：:Accumulation of carboxymethylated proteins (CML-proteins) is taken as a biomarker of glycoxidative stress which is thought to contribute to the age-related impairment in tissue and cell function. To investigate the occurrence and extent of glycoxidative damage with aging in rat kidney, serum and urine, we have prepare...

journal_title：Archives of biochemistry and biophysics

authors： Hamelin M,Borot-Laloi C,Friguet B,Bakala H

更新日期：2003-03-15 00:00:00

abstract：:Apigenin is a representative dietary flavone (2-phenyl-4H-1-benzopyran-4-one) inhibiting cancer cell growth both in cell culture systems and in vivo. The prooxidant potential of apigenin was confirmed by the observations using flowcytometric and immunoblotting techniques that the intracellular accumulations of reactiv...

journal_title：Archives of biochemistry and biophysics

authors： Miyoshi N,Naniwa K,Yamada T,Osawa T,Nakamura Y

更新日期：2007-10-15 00:00:00

abstract：:Anthocyanins are the main group of natural hydrosoluble pigments in plants. They introduce colouring to foods, with colours ranging from blue to red and orange. Nowadays, their importance for the Food and Pharmaceutical industries is mainly based in the existing scientific work evidencing their beneficial effects on t...

journal_title：Archives of biochemistry and biophysics

authors： de Pascual-Teresa S

更新日期：2014-10-01 00:00:00

abstract：:Renally excreted 8-oxo-7,8-dihydro-2(')-deoxyguanosine (oxo(8)dG) is a potential marker of oxidative DNA damage by reactive oxygen species. Whole-body degradation rates of t- and rRNA are potential indicators of the resting metabolic rate (RMR). Excretion rates of oxo(8)dG and degradation rates of t- and rRNA were det...

journal_title：Archives of biochemistry and biophysics

authors： Topp H,Armbrust S,Lengger C,Schöch G,Davies J,Stichler W,Manz F,Fusch C

更新日期：2002-06-01 00:00:00

abstract：:CopA is a protein formed as part of a copper resistance operon in Pseudomonas syringae pv tomato, but CopA has also been identified from gene library screening as a potential lignin-oxidising enzyme. Few bacterial homologues for bacterial multi-copper laccases have been identified that can assist in lignin degradation...

journal_title：Archives of biochemistry and biophysics

authors： Granja-Travez RS,Bugg TDH

更新日期：2018-12-15 00:00:00

abstract：:CYP119 from Sulfolobus solfataricus is the first well-characterized thermophilic cytochrome P450 enzyme. The endogenous substrate for this enzyme is not known but it hydroxylates lauric acid in a reaction supported by surrogate mesophilic electron donors. However, reconstitution of a high-temperature catalytic system ...

journal_title：Archives of biochemistry and biophysics

authors： Puchkaev AV,Ortiz de Montellano PR

更新日期：2005-02-01 00:00:00

abstract：:The electron paramagnetic (EPR) properties of a transient species detected during a cytochrome P450 (P450)-mediated peroxidative reaction have been compared with those of peroxidases Compound I and model metalloporphyrins. The reaction which was studied with cholesterol-specific P450scc and (20R)-20-hydroperoxycholest...

journal_title：Archives of biochemistry and biophysics

authors： Larroque C,Lange R,Maurin L,Bienvenue A,van Lier JE

更新日期：1990-10-01 00:00:00

abstract：:Biotin sulfoxide reductase catalyzes the conversion of d-biotin d-sulfoxide (BSO) to d-biotin. Oligonucleotides directed against common sequences in Escherichia coli biotin sulfoxide reductase and in Rhodobacter sphaeroides f.sp. denitrificans dimethyl sulfoxide reductase have been utilized to amplify by PCR a 651-bp ...

journal_title：Archives of biochemistry and biophysics

authors： Pollock VV,Barber MJ

更新日期：1995-04-20 00:00:00

abstract：:Purified NAD-malic enzyme from Ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mM borate. Determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedio...

journal_title：Archives of biochemistry and biophysics

authors： Rao GS,Kong CT,Benjamin RC,Harris BG,Cook PF

更新日期：1987-05-15 00:00:00

abstract：:Three major pathways mediate amino acid transport into mammalian cells: the A-system and the ASC-system, which require a sodium gradient across the plasma membrane, and the L-system, which has no requirement for a sodium gradient. We have found that the lymphocytes from patients with B-cell chronic lymphocytic leukemi...

journal_title：Archives of biochemistry and biophysics

authors： Segel GB,Tometsko AM,Lichtman MA

更新日期：1985-11-01 00:00:00

abstract：:The steady-state kinetic mechanism of vitamin K-dependent carboxylase from calf liver has been investigated by initial-velocity measurements with varying concentrations of two carboxylase substrates and constant, nonsaturating concentrations of the other two substrates. With all combinations of the varied substrates t...

journal_title：Archives of biochemistry and biophysics

authors： Uotila L

更新日期：1988-07-01 00:00:00

abstract：:It is established that high concentrations of nitric oxide(1) (NO), as released from activated macrophages, induce apoptosis in breast cancer cells. In this study, we assessed the potential of a light-activated NO donor [(Me2bpb)Ru(NO)(Resf)], a recently reported apoptototic agent, in suppressing the anchorage indepen...

journal_title：Archives of biochemistry and biophysics

authors： Sen S,Kawahara B,Fry NL,Farias-Eisner R,Zhang D,Mascharak PK,Chaudhuri G

更新日期：2013-12-01 00:00:00

abstract：:Purification of equilibrative nucleoside transporters has been hampered by the functional instability of the detergent-solubilized proteins. A variety of nonionic detergents were compared with octylglucoside (the most commonly used detergent in this regard) for their abilities to solubilize functionally stable nucleos...

journal_title：Archives of biochemistry and biophysics

authors： Hammond JR,Zarenda M

更新日期：1996-08-15 00:00:00

abstract：:Systemic exposure to gram-negative bacterial substances such as lipopolysaccharide (LPS, or endotoxin) induces an uncontrolled, massive inflammatory reaction which culminates in multiple system organ failure and death. Septic shock often does not respond to corticosteroids; however, certain low-molecular-weight antiox...

journal_title：Archives of biochemistry and biophysics

authors： Stewart CA,Hyam K,Wallis G,Sang H,Robinson KA,Floyd RA,Kotake Y,Hensley K

更新日期：1999-05-01 00:00:00

abstract：:The rates of reactive absorption of ozone by various biomolecule solutions were measured. At pH 7, the ability of various biomolecules to reactively absorb ozone was in the following sequence: thiosulfate > ascorbate > cysteine approximately methionine > glutathione. The rates of reactive absorption under a variety of...

journal_title：Archives of biochemistry and biophysics

authors： Kanofsky JR,Sima PD

更新日期：1995-01-10 00:00:00

abstract：:The retinal pigment epithelium (RPE)-specific protein RPE65 is the major protein of the RPE microsomal membrane fraction. Though RPE65 lacks transmembrane domains or signal peptide, detergents are required for its maximally effective solubilization in isotonic buffers. However, in 0.75-1.0 M KCl, RPE65 is as soluble w...

journal_title：Archives of biochemistry and biophysics

authors： Tsilou E,Hamel CP,Yu S,Redmond TM

更新日期：1997-10-01 00:00:00

abstract：:The earthworm-derived chemoattractant ES20 interacts with its G-protein-coupled receptors on the plasma membrane of vomeronasal (VN) sensory neurons of garter snakes, resulting in an increase in inositol trisphosphate [J. Biol. Chem. 269 (1994) 16867] and a rapid phosphorylation of the membrane-bound proteins, p42/44 ...

journal_title：Archives of biochemistry and biophysics

authors： Wang D,Liu W,Liu J,Chen P,Quan W,Halpern M

更新日期：2002-12-15 00:00:00

abstract：:Heme-catalyzed decomposition of unsaturated hydroperoxy fatty acids has been proposed to proceed via carbon-centered free radicals (delocalized at positions C11, C12, and C13 for 15-hydroperoxy-eicosatetraenoic acid (15-HPETE). The stable products are usually epoxy fatty acids and epoxy alcohols. Hydroperoxides from a...

journal_title：Archives of biochemistry and biophysics

authors： Schreiber J,Mason RP,Eling TE

更新日期：1986-11-15 00:00:00

abstract：:Preparations of one-chain tissue plasminogen activator (tPA), usually a mixture of 65- and 63-kDa differentially glycosylated forms, contain variable amounts of two-chain tPA. There is no effective procedure currently available for removal of the two-chain contaminant from one-chain tPA preparations. In this report, a...

journal_title：Archives of biochemistry and biophysics

authors： Husain SS

更新日期：1991-03-01 00:00:00

abstract：:The combination of phorbol 12-myristate 13-acetate (PMA) and ionomycin produces a dramatic increase in the incorporation of [2-3H]mannose into Glc3Man9GlcNAc2-P-P-dolichol and glycoprotein, and the induction of RNA and DNA synthesis in murine splenic B lymphocytes (B cells). The kinetics of the induction processes and...

journal_title：Archives of biochemistry and biophysics

authors： Rush JS,Snow EC,Waechter CJ

更新日期：1988-11-15 00:00:00

abstract：:Vascular dysfunction and injurious stimuli such as oxidative stress is closely related to the risk of cardiovascular diseases (CVD). Dietary polyphenols is reported to exert the beneficial effects on reducing the risk of CVD. Black soybean is rich in polyphenols, including isoflavones, anthocyanidins and flavan-3-ols,...

journal_title：Archives of biochemistry and biophysics

authors： Yamashita Y,Wang L,Nakamura A,Nanba F,Saito S,Toda T,Nakagawa J,Ashida H

更新日期：2020-07-30 00:00:00

abstract：:The crystalline beef liver protein of Sumner and Dounce (A. L. Dounce, P. Z. Allen, and G. A. Mourtzikos (1978) Arch. Biochem. Biophys. 188, 251-265) termed FTBL (football) protein because of the shape of its crystals, has been identified as a crystalline leucine aminopeptidase (LAP), on the basis of its high specific...

journal_title：Archives of biochemistry and biophysics

authors： Dounce AL,Allen PZ

更新日期：1987-08-15 00:00:00

abstract：:A new procedure for the purification of phospholipase C from Clostridium perfringens has been devised that results in essentially pure enzyme. The procedure consists of ammonium sulfate fractionation, ion-exchange chromatography on QAE-Sephadex, and affinity chromatography on phosphatidylcholine linked to Sepharose. T...

journal_title：Archives of biochemistry and biophysics

authors： Krug EL,Kent C

更新日期：1984-06-01 00:00:00

abstract：:Most important processes in cell life are regulated by calcium (Ca2+). A number of mechanisms have thus been developed to maintain the concentration of free Ca2+ inside cells at the level (100-200nM) necessary for the optimal operation of the targets of its regulatory function. The systems that move Ca2+ back and fort...

journal_title：Archives of biochemistry and biophysics

authors： Di Leva F,Domi T,Fedrizzi L,Lim D,Carafoli E

更新日期：2008-08-01 00:00:00

abstract：:The conformational behavior of receptor-bound acetylcholine (ACh) was investigated by molecular dynamics simulations. Based on the great similarity among muscarinic receptors, the study was focused on the human M(1), M(2), and M(5) receptors as previously modeled by us. The results showed that receptor-bound ACh was n...

journal_title：Archives of biochemistry and biophysics

authors： Vistoli G,Pedretti A,Testa B,Matucci R

更新日期：2007-08-01 00:00:00

abstract：:Nucleotide Binding Domains (NBDs) are responsible for the ATPase activity of the multidrug resistance protein 1 (MRP1). A series of NBD1-linker-NBD2 chimeric fusion proteins were constructed, expressed and purified, and their ATPase activities were analyzed. We report here that a GST linked NBD1(642-890)-GST-NBD2(1286...

journal_title：Archives of biochemistry and biophysics

authors： Wan L,Liang X,Huang Y

更新日期：2009-05-15 00:00:00

abstract：:The role of the conserved sequence motif 301DDLTDP306 in the F0F1 ATP synthase beta subunit was assessed by mutagenic analysis in the Escherichia coli enzyme. Mutations gave variable effects on F1 sector activity, stability, and membrane binding to the F0 sector. Upon solubilization, F1 sectors of the betaD302E and be...

journal_title：Archives of biochemistry and biophysics

authors： Omote H,Tainaka K,Fujie K,Iwamoto-Kihara A,Wada Y,Futai M

更新日期：1998-10-15 00:00:00

abstract：:The oxidation of quercetin by horseradish peroxidase/H(2)O(2) was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the absence of GSH revealed formation of at least 20 different products, a result in line with other studies reporting the ...

journal_title：Archives of biochemistry and biophysics

authors： Awad HM,Boersma MG,Vervoort J,Rietjens IM

更新日期：2000-06-15 00:00:00

abstract：:An in vitro expression plasmid (pGRAP) that contained the cDNA coding for the rat mitochondrial aldehyde dehydrogenase precursor was constructed, mRNA was synthesized then translated, and the in vitro synthesized precursor of aldehyde dehydrogenase was used in an in vitro import assay. As expected the 19 amino acid si...

journal_title：Archives of biochemistry and biophysics

authors： Wang TT,Farrés J,Weiner H

更新日期：1989-08-01 00:00:00