RPE65, the major retinal pigment epithelium microsomal membrane protein, associates with phospholipid liposomes.

abstract：:Dethiolation of proteins (reduction of protein mixed disulfides) by NADPH-dependent and glutathione (GSH)-dependent enzymes, and by nonenzymatic reaction with GSH, was studied by electrofocusing methodology with glycogen phosphorylase b and creatine kinase as substrates. Phosphorylase b was not rapidly dethiolated by ...

journal_title：Archives of biochemistry and biophysics

authors： Park EM,Thomas JA

更新日期：1989-10-01 00:00:00

abstract：:The ability of chelators and ascorbic acid to mobilize iron from crocidolite, amosite, medium- and short-fiber chrysotile, and tremolite was investigated. Ferrozine, a strong Fe(II) chelator, mobilized Fe(II) from crocidolite (6.6 nmol/mg asbestos/h) and amosite (0.4 nmol/mg/h) in 50 mM NaCl, pH 7.5. Inclusion of asco...

journal_title：Archives of biochemistry and biophysics

authors： Lund LG,Aust AE

更新日期：1990-04-01 00:00:00

abstract：:We have previously shown that exposure of Clone 9 cells to hypoxia, cyanide, or azide results in an acute stimulation of glucose transport that is largely mediated by "activation" of glucose transporter (Glut1) sites preexisting in the plasma membrane. However, it is not known whether inhibition of oxidative phosphory...

journal_title：Archives of biochemistry and biophysics

authors： Hamrahian AH,Zhang JZ,Elkhairi FS,Prasad R,Ismail-Beigi F

更新日期：1999-08-15 00:00:00

abstract：:Tryparedoxin peroxidase (TXNPx) of Trypanosomatidae is the terminal peroxidase of a complex redox cascade that detoxifies hydroperoxides by NADPH (Nogoceke et al., Biol. Chem. 378, 827-836, 1997). A gene putatively coding for a peroxiredoxin-type TXNPx was identified in L. donovani and expressed in Escherichia coli to...

journal_title：Archives of biochemistry and biophysics

authors： Flohé L,Budde H,Bruns K,Castro H,Clos J,Hofmann B,Kansal-Kalavar S,Krumme D,Menge U,Plank-Schumacher K,Sztajer H,Wissing J,Wylegalla C,Hecht HJ

更新日期：2002-01-15 00:00:00

abstract：:Serum acetone in neonates was found to increase from < 20 microM at 20 days gestation to 377 +/- 107 microM 1 day after birth. This increase in acetone occurs concurrently with the initial expression of liver P450IIE1 in rat (Song et al., 1986, J. Biol. Chem. 261:16689-16697). Treating pregnant rats with drinking wate...

journal_title：Archives of biochemistry and biophysics

authors： Casazza JP,Sohn DH,Park KS,Song BJ

更新日期：1994-02-15 00:00:00

abstract：:Peroxynitrite, a reactive cytotoxic species generated by the reaction of superoxide with nitric oxide, rapidly oxidizes phenylaminoethyl selenide (PAESe) and its para-substituted derivatives with second-order rate constants ranging from 900 to 3000 M(-1) s(-1) at neutral pH (pH 7.0) and 25 degrees C. These values are ...

journal_title：Archives of biochemistry and biophysics

authors： Woznichak MM,Overcast JD,Robertson K,Neumann HM,May SW

更新日期：2000-07-15 00:00:00

abstract：:Recognition of defined carbohydrate structures by boar sperm was studied on the basis of oligosaccharide structures of porcine zona pellucida glycoproteins so far elucidated. Boar sperm abundantly adhered to fetuin-Sepharose beads, moderately to asialofetuin-Sepharose beads, but not at all to galactosidase (beta1-4-li...

journal_title：Archives of biochemistry and biophysics

authors： Mori E,Yoshitani N,Mori T,Takasaki S

更新日期：2000-02-01 00:00:00

abstract：:Ion channel-protein complexes inserted in the membrane act as molecular gates for transport across the membrane. The opening and closing of these gates can be controlled by one or more variables like ligands (small molecules, proteins, etc.), transmembrane voltage, and the concentration gradient of a chemical across t...

journal_title：Archives of biochemistry and biophysics

authors： Banerjee J,Ghosh S

更新日期：2005-03-15 00:00:00

abstract：:Two complementary DNA (cDNA) clones (pTK-1 and -2) encoding two distinct isotypes of mouse Mg(2+)-dependent protein phosphatase beta (MPP beta-1 and -2, respectively) were isolated from a melanocyte cDNA library. Although mouse pTK-1 is orthologous to the rat cDNA (JW5) reported previously [Wenk, J., Trompeter, H.I., ...

journal_title：Archives of biochemistry and biophysics

authors： Terasawa T,Kobayashi T,Murakami T,Ohnishi M,Kato S,Tanaka O,Kondo H,Yamamoto H,Takeuchi T,Tamura S

更新日期：1993-12-01 00:00:00

abstract：:The facultative intracellular bacterial pathogen Listeria monocytogenes invades and multiplies in many mammalian cell types. During the interaction with its host cells it strongly interferes with and modulates host cell functions. In the present review we summarize the current knowledge on the modulation of signal tra...

journal_title：Archives of biochemistry and biophysics

authors： Kuhn M,Pfeuffer T,Greiffenberg L,Goebel W

更新日期：1999-12-01 00:00:00

abstract：:It was shown previously that the ATP synthase complex of bovine heart mitochondria contains an essential set of thiols or dithiols in its membrane sector (F0), whose modification by various reagents results in uncoupling [Yagi, T., and Hatefi, Y. (1984) Biochemistry 23, 2449-2455]. The sensitivity to modifiers was inc...

journal_title：Archives of biochemistry and biophysics

authors： Yagi T,Hatefi Y

更新日期：1987-04-01 00:00:00

abstract：:Betaine-homocysteine methyltransferase (BHMT) is a member of a family (Pfam 02574) of zinc- and thiol/selenol-dependent methyltransferases. All family members purified to date are monomers, except BHMT, which is an oligomer. We have studied how C-terminal truncation or mutagenic replacement of residues within or assoc...

journal_title：Archives of biochemistry and biophysics

authors： Szegedi SS,Garrow TA

更新日期：2004-06-01 00:00:00

abstract：:7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) is a specific covalent inhibitor of P-glycoprotein ATPase activity (M. K. Al-Shawi, and A. E. Senior, 1993, J. Biol. Chem. 268, 4197-4206). Complete inhibition occurs at a reaction stoichiometry of 1 mol NBD/mol P-glycoprotein, and the reagent has proved valuable in und...

journal_title：Archives of biochemistry and biophysics

authors： Senior AE,Gros P,Urbatsch IL

更新日期：1998-09-01 00:00:00

abstract：:The aim of this study was to investigate the effect of insulin resistance on glycogen concentration and glycogen synthase activity in the red and white gastrocnemius muscles and to determine whether the inverse relationship existing between glycogen concentration and enzyme activity is maintained in insulin resistant ...

journal_title：Archives of biochemistry and biophysics

authors： Coderre L,Vallega GA,Pilch PF,Chipkin SR

更新日期：2007-08-01 00:00:00

abstract：:Reversible oxidation of Cys residues is a crucial element of redox homeostasis and signaling. According to a popular concept in oxidative stress signaling, the oxidation of targets of signals can only take place following an overwhelming of the cellular antioxidant capacity. This concept, however, ignores the activati...

journal_title：Archives of biochemistry and biophysics

authors： Zaccarin M,Bosello-Travain V,Di Paolo ML,Falda M,Maiorino M,Miotto G,Piccolo S,Roveri A,Ursini F,Venerando R,Toppo S

更新日期：2017-03-01 00:00:00

abstract：:This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol-protein ratio (C/Pt). The membrane fluidity, as assessed by a ...

journal_title：Archives of biochemistry and biophysics

authors： Dumas D,Muller S,Gouin F,Baros F,Viriot ML,Stoltz JF

更新日期：1997-05-01 00:00:00

abstract：:In this study, oxygen consumption and H(2)O(2) release rate by succinate or pyruvate/malate supplemented mitochondria isolated from skeletal muscle of trained and untrained rats were investigated. The overall mitochondrial antioxidant capacity and the effect of preincubation of mitochondria with GDP, an inhibitor of u...

journal_title：Archives of biochemistry and biophysics

authors： Venditti P,Masullo P,Di Meo S

更新日期：1999-12-15 00:00:00

abstract：:The parent pyridine nucleotide NAD is the end product of oxidative tryptophan catabolism via the kynurenine pathway. Indoleamine 2, 3-dioxygenase, the rate-limiting enzyme for this pathway, is induced by the proinflammatory cytokine interferon-gamma. The aim of this study was to investigate the effect of interferon-ga...

journal_title：Archives of biochemistry and biophysics

authors： Grant RS,Passey R,Matanovic G,Smythe G,Kapoor V

更新日期：1999-12-01 00:00:00

abstract：:Infrared spectroscopy was used to investigate the changes in bands assigned to phospholipids and proteins in dehydrated and rehydrated sarcoplasmic reticulum. The changes in CH2 and CH3 stretching bands, amide bands, and phosphate stretching bands are similar to shifts in frequency seen for those bands in phospholipid...

journal_title：Archives of biochemistry and biophysics

authors： Crowe JH,Crowe LM,Chapman D

更新日期：1984-07-01 00:00:00

abstract：:Incubation of either Chlorella nitrate reductase or the recombinant flavin domain of spinach nitrate reductase with reagents specific for modification of cysteine residues, such as N-ethylmaleimide, resulted in a time-dependent inactivation of NADH:ferricyanide reductase activity which could be prevented by incubation...

journal_title：Archives of biochemistry and biophysics

authors： Trimboli AJ,Quinn GB,Smith ET,Barber MJ

更新日期：1996-07-01 00:00:00

abstract：:If liver alcohol dehydrogenase were rate-limiting in ethanol metabolism, inhibitors of the enzyme should inhibit the metabolism with the same type of kinetics and the same kinetic constants in vitro and in vivo. Against varied concentrations of ethanol, 4-methylpyrazole is a competitive inhibitor of purified rat liver...

journal_title：Archives of biochemistry and biophysics

authors： Plapp BV,Leidal KG,Smith RK,Murch BP

更新日期：1984-04-01 00:00:00

abstract：:The delta pH in ionophore-treated cells of an acidophile has been determined by electron spin resonance spectroscopy. The values obtained were comparable to those obtained using the more conventional techniques involving radiolabeled probes. No binding of the spin-labeled probe was observed as determined by two indepe...

journal_title：Archives of biochemistry and biophysics

authors： McLaggan D,Belkin S,Packer L,Matin A

更新日期：1989-08-15 00:00:00

abstract：:SH2 domain-containing inositol 5-phosphatase 2 (SHIP2) binds with the Y1356-phosphorylated hepatocyte growth factor (HGF) receptor, c-MET, through its SH2 domain, which is essential for the role of SHIP2 in HGF-induced cell scattering and cell spreading. Previously, the experimental structure of the SH2 domain from SH...

journal_title：Archives of biochemistry and biophysics

authors： Wang Z,Nie Y,Zhang K,Xu H,Ramelot TA,Kennedy MA,Liu M,Zhu J,Yang Y

更新日期：2018-10-15 00:00:00

abstract：:Several 2-halo-acyl-CoA derivatives have been synthesized to examine the interaction of these potential inhibitors of mitochondrial beta-oxidation with the purified medium chain acyl-CoA dehydrogenase from pig kidney. Racemic 2-bromo-, 2-chloro-, and 2-fluoro-octanoyl-CoA thioesters show 6.6, 33, and 3.5% of the activ...

journal_title：Archives of biochemistry and biophysics

authors： Cummings JG,Thorpe C

更新日期：1993-04-01 00:00:00

abstract：:Studies have demonstrated that accumulation of mitochondrial tocopheroxyl radical, the primary oxidation product of alpha-tocopherol, accompanies rapid consumption of tocopherol. Enzyme-linked electron flow lowers both the steady-state concentration of the radical and the consumption of tocopherol. Reduction of tocoph...

journal_title：Archives of biochemistry and biophysics

authors： Maguire JJ,Kagan V,Ackrell BA,Serbinova E,Packer L

更新日期：1992-01-01 00:00:00

abstract：:A new creatine analog, 1-carboxyethyl-2-iminoimidazolidine (homocyclocreatine), has been synthesized and compared with other synthetic analogs of creatine as a substrate for creatine kinase under both in vitro and in vivo conditions. Reactivity with rabbit muscle creatine kinase at 2 mM and pH 7.0 occurred in the orde...

journal_title：Archives of biochemistry and biophysics

authors： Roberts JJ,Walker JB

更新日期：1983-02-01 00:00:00

abstract：:In the Escherichia coli class Ia ribonucleotide reductase (RNR), the best characterized RNR, there is no spectroscopic evidence for the existence of the postulated catalytically essential thiyl radical (R-S(*)) in the substrate binding subunit R1. We report first results on artificially generated thiyl radicals in R1 ...

journal_title：Archives of biochemistry and biophysics

authors： Kolberg M,Bleifuss G,Sjöberg BM,Gräslund A,Lubitz W,Lendzian F,Lassmann G

更新日期：2002-01-01 00:00:00

abstract：:Carotenoids possessing hydroxyl groups can be found in nature both as free xanthophylls and as carotenoid esters, i.e. acylated with fatty acids. A single carotenoid can be acylated with different fatty acids, resulting in a high number of possible structures of carotenoid esters. The analysis of carotenoid esters can...

journal_title：Archives of biochemistry and biophysics

authors： Mariutti LRB,Mercadante AZ

更新日期：2018-06-15 00:00:00

abstract：:Paxilline, an indole alkaloid mycotoxin from Penicillium paxilli, is an inhibitor of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA). Paxilline inhibited differing isoforms of SERCA with IC50s between 5 and 50 microM. It inhibited more potently the purified Ca2+ ATPase activity from skeletal muscle with an IC50 of...

journal_title：Archives of biochemistry and biophysics

authors： Bilmen JG,Wootton LL,Michelangeli F

更新日期：2002-10-01 00:00:00

abstract：:Oligopeptidase A (OpdA) belongs to the M3A subfamily of bacterial peptidases with catalytic and structural properties similar to mammalian thimet-oligopeptidase (TOP) and neurolysin (NEL). The three enzymes have four conserved Tyr residues on a flexible loop in close proximity to the catalytic site. In OpdA, the flexi...

journal_title：Archives of biochemistry and biophysics

authors： Lorenzon RZ,Cunha CE,Marcondes MF,Machado MF,Juliano MA,Oliveira V,Travassos LR,Paschoalin T,Carmona AK

更新日期：2010-08-15 00:00:00