Properties and subcellular localization of adenosine diphosphatase in rat heart.

abstract：:Previous studies demonstrated that liver microsomes from untreated rats catalyze the omega, omega-1, and omega-2 hydroxylation of prostaglandins [K. A. Holm, R. J. Engell, and D. Kupfer (1985) Arch. Biochem. Biophys. 237, 477-489]. The current study examined the regioselectivity of hydroxylation of PGE1 and PGE2 by pu...

journal_title：Archives of biochemistry and biophysics

authors： Kupfer D,Jansson I,Favreau LV,Theoharides AD,Schenkman JB

更新日期：1988-02-15 00:00:00

abstract：:Partially purified linamarase from Trifolium repens (genotype Lili acac) plants was kinetically characterized. Kinetic evidence was found to support the assumption that this cyanogenic beta-D-glucosidase has a broad substrate spectrum. p-Nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinopyranoside sub...

journal_title：Archives of biochemistry and biophysics

authors： Pócsi I,Kiss L,Hughes MA,Nánási P

更新日期：1989-08-01 00:00:00

abstract：:A biochemical basis for the pea and lentil lectin resistance of two Chinese hamster ovary (CHO) cell mutants, Lec13 and Lec13A, was investigated. Studies of the G glycopeptides of vesicular stomatitis virus grown in the mutants indicated that Lec13 cells essentially lack the ability to add fucose to complex carbohydra...

journal_title：Archives of biochemistry and biophysics

authors： Ripka J,Adamany A,Stanley P

更新日期：1986-09-01 00:00:00

abstract：:Soybean Bowman-Birk protease inhibitor (BBI) is an inhibitor of serine proteases with two functional inhibitory domains of different specificities: one is specific for chymotrypsin-like proteases, the other for trypsin-like proteases. Chymase and tryptase are serine proteases which are stored in mast cell granules and...

journal_title：Archives of biochemistry and biophysics

authors： Ware JH,Wan XS,Rubin H,Schechter NM,Kennedy AR

更新日期：1997-08-01 00:00:00

abstract：:Previous work from this laboratory indicated that the N-terminus of recombinant human cytochrome P450 (P450) 1A2 expressed in Escherichia coli is blocked (P. Sandhu, Z. Guo, T. Baba, M. V. Martin, R. H. Tukey, and F. P. Guengerich, (1994) Arch. Biochem. Biophys. 30, 168 -177). A modification of this construct was done...

journal_title：Archives of biochemistry and biophysics

authors： Dong MS,Yamazaki H,Guo Z,Guengerich FP

更新日期：1996-03-01 00:00:00

abstract：:CYP3A9 is an estrogen-inducible ortholog of human liver CYP3A4 with 76.5% sequence identity to CYP3A4. Unlike CYP3A4, it is a very poor testosterone 6beta- and 2beta-hydroxylase, but a relatively better catalyst of progesterone monohydroxylation largely at 6beta, 16alpha, and 21 positions with negligible 6beta, 21-dih...

journal_title：Archives of biochemistry and biophysics

authors： Xue L,Zgoda VG,Arison B,Correia MA

更新日期：2003-01-01 00:00:00

abstract：:The tumor-promoting phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) inhibited thrombin-stimulated arachidonic acid (AA) release in rabbit and human platelets. PMA was effective over the same concentration range that activates protein kinase C in intact rabbit platelets: IC50 vs thrombin = 0.5 nM, greater th...

journal_title：Archives of biochemistry and biophysics

authors： Murayama T,Kajiyama Y,Takahashi A,Nomura Y

更新日期：1990-01-01 00:00:00

abstract：:Peroxiredoxin 6 (Prdx6) differs from other mammalian peroxiredoxins both in its ability to reduce phospholipid hydroperoxides at neutral pH and in having phospholipase A(2) (PLA(2)) activity that is maximal at acidic pH. We previously showed an active site C47 for peroxidase activity and a catalytic triad S32-H26-D140...

journal_title：Archives of biochemistry and biophysics

authors： Manevich Y,Shuvaeva T,Dodia C,Kazi A,Feinstein SI,Fisher AB

更新日期：2009-05-15 00:00:00

abstract：:The interconversion of mannose-6-P and mannose-1-P in brain has been shown to be catalyzed by a distinct enzyme. The enzyme has been separated from most of the phosphoglucomutase activity of the brain. The residual phosphoglucomutase activity (less than 1%) may be associated with phosphomannomutase itself. Mannose-1,6...

journal_title：Archives of biochemistry and biophysics

authors： Guha SK,Rose ZB

更新日期：1985-11-15 00:00:00

abstract：:Nucleotide-free kinesin motor domains from several kinesin families convert reversibly to a refractory conformation that cannot rapidly rebind ADP. In the absence of glycerol, the refractory conformation of Drosophila kinesin motor domains is favored by 50-fold with conversion of the active to the refractory species a...

journal_title：Archives of biochemistry and biophysics

authors： Hackney DD,McGoff MS

更新日期：2016-10-15 00:00:00

abstract：BACKGROUND:Proteomics is expected to provide novel insights in the underlying pathophysiology of type 2 diabetes mellitus. In the present study, we aimed to identify and biochemically characterize proteins associated with diabetes mellitus in a Qatari population. METHODS:In a diabetes case-control study (175 cases, 16...

journal_title：Archives of biochemistry and biophysics

authors： Noordam R,van Heemst D,Suhre K,Krumsiek J,Mook-Kanamori DO

更新日期：2020-08-15 00:00:00

abstract：:We have previously shown that rat hepatic cytochromes P450f, P450g, P450h, and P450i possess a high degree of immunochemical and, presumably, structural relatedness. Polyclonal antibodies directed against cytochromes P450f and P450g were made monospecific by immunoabsorption against the cross-reactive proteins. The sp...

journal_title：Archives of biochemistry and biophysics

authors： Bandiera S,Ryan DE,Levin W,Thomas PE

更新日期：1986-08-01 00:00:00

abstract：:Two Escherichia coli mutants that lack both cytochrome o and d terminal oxidases are able to grow with glucose as the carbon source but not with the aerobic substrates succinate or lactate. One of these, GV101, is a deletion mutant of cytochrome o and a point mutation of cytochrome d. The other, GK100, is a total dele...

journal_title：Archives of biochemistry and biophysics

authors： Dikshit RP,Dikshit KL,Liu YX,Webster DA

更新日期：1992-03-01 00:00:00

abstract：:Incubation of rat liver nuclear envelopes with [gamma-32P]ATP resulted in the synthesis of phosphatidylinositol-[4-32P]phosphate (PIP). Degradation of endogenously labeled PIP was observed upon the dilution of the labeled ATP with an excess of unlabeled ATP. This degradation was most rapid in the presence of EDTA, and...

journal_title：Archives of biochemistry and biophysics

authors： Smith CD,Wells WW

更新日期：1984-12-01 00:00:00

abstract：:The sites of expression in the small intestine and the function of CYP2J4, a recently identified rat cytochrome (P450) isoform found to be predominantly expressed in the small intestine, were characterized. Immunoblot analysis with a polyclonal antibody to heterologously expressed CYP2J4 revealed that expression of CY...

journal_title：Archives of biochemistry and biophysics

authors： Zhang QY,Raner G,Ding X,Dunbar D,Coon MJ,Kaminsky LS

更新日期：1998-05-15 00:00:00

abstract：:Prior exposure of guinea pig macrophages to LPS (lipopolysaccharide) resulted in reduced cAMP-generating responses to prostaglandin E1 and epinephrine. LPS-induced refractoriness was diminished when LPS treatment was carried out in the presence of an inhibitor of prostaglandin synthesis, hydrocortisone, or indomethaci...

journal_title：Archives of biochemistry and biophysics

authors： Hazeki K,Mori Y,Ui M

更新日期：1986-05-01 00:00:00

abstract：:Various proteins sharing thioredoxin (Trx)-like active site sequences (Cys-Xxx-Xxx-Cys) have been found and classified in the Trx superfamily. Among them, transmembrane Trx-related protein (TMX) was recently identified as a novel protein possessing an atypical active site sequence, Cys-Pro-Ala-Cys. In the present stud...

journal_title：Archives of biochemistry and biophysics

authors： Matsuo Y,Nishinaka Y,Suzuki S,Kojima M,Kizaka-Kondoh S,Kondo N,Son A,Sakakura-Nishiyama J,Yamaguchi Y,Masutani H,Ishii Y,Yodoi J

更新日期：2004-03-01 00:00:00

abstract：:Thermal perturbation techniques have been used to probe structural features of the nicotinic acetylcholine receptor (AcChR). The information obtained from differential scanning calorimetry (DSC) of AcChR membranes (M.C. Farach and M. Martinez-Carrion (1983) J. Biol. Chem. 258, 4176) in the absence and in the presence ...

journal_title：Archives of biochemistry and biophysics

authors： Artigues A,Villar MT,Ferragut JA,Gonzalez-Ros JM

更新日期：1987-10-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae SOC8 DNA fragment was isolated as a wildtype dominant suppressor of the cdc8 mutation. The SOC8 has previously been proved to be allelic with URA6, encoding the UMP kinase in yeast cells. The protein has been purified to homogeneity. In this report, we describe the characteristics of...

journal_title：Archives of biochemistry and biophysics

authors： Jong A,Yeh Y,Ma JJ

更新日期：1993-07-01 00:00:00

abstract：:Proteins containing the post-translationally modified amino acid L-3,4-dihydroxyphenylalanine (DOPA) undergo autosclerotization as a means of assuring cohesive resilience in many structural matrices found in nature. To explore the chemical mechanism of sclerotization, we examined the oxidation products of relatively s...

journal_title：Archives of biochemistry and biophysics

authors： Rzepecki LM,Nagafuchi T,Waite JH

更新日期：1991-02-15 00:00:00

abstract：:Periodate treatments of apo human serum transferrin (HST), and apo chicken ovotransferrin (COT) were previously reported to cause a rapid loss of Fe+3 binding capacity, with a loss of 3 to 5 tyrosine residues [P. AZARI AND J. L. PHILLIPS (1970) Arch. Biochem. Biophys. 138, 32-38; K. F. GEOGHEGAN, J. L. DALLAS, AND R. ...

journal_title：Archives of biochemistry and biophysics

authors： Penner MH,Yamasaki RB,Osuga DT,Babin DR,Meares CF,Feeney RE

更新日期：1983-09-01 00:00:00

abstract：:Transport of the antifolate cancer drug methotrexate was studied in vesicles isolated from the basolateral membrane of rat liver. Transport of methotrexate by basolateral membrane vesicles (BLMVs) was mostly via uptake into an osmotically active intravesicular space, with some binding (approximately 9%), as shown by i...

journal_title：Archives of biochemistry and biophysics

authors： Horne DW,Reed KA

更新日期：1992-10-01 00:00:00

abstract：:The multifunctional tyrosine N-hydroxylase, cytochrome P450TYR (CYP79), from Sorghum bicolor catalyzing the conversion of tyrosine to p-hydroxyphenyl-acetaldoxime in the biosynthesis of the cyanogenic glucoside dhurrin, has been expressed in Escherichia coli using the isopropyl-beta-D-thiogalactopyranoside-inducible v...

journal_title：Archives of biochemistry and biophysics

authors： Halkier BA,Nielsen HL,Koch B,Møller BL

更新日期：1995-10-01 00:00:00

abstract：:Lipopolysaccharide (LPS) increases the levels of the endogenous cannabinoid anandamide (N-arachidonoylethanolamine, AEA) in rat macrophages, but the mechanism responsible for this effect has not been elucidated. Here we demonstrate that LPS enhances the levels of AEA (fourfold over controls) also in human lymphocytes....

journal_title：Archives of biochemistry and biophysics

authors： Maccarrone M,De Petrocellis L,Bari M,Fezza F,Salvati S,Di Marzo V,Finazzi-Agrò A

更新日期：2001-09-15 00:00:00

abstract：:Lipid homeostasis in liver is known to be altered with diabetes mellitus, ultimately leading to liver damage and related complications. The present work aimed to evaluate changes in the liver phospholipid profile after 4 months of uncontrolled hyperglycemia. Twenty Wistar rats were divided into two groups: control and...

journal_title：Archives of biochemistry and biophysics

authors： Simões C,Domingues P,Ferreira R,Amado F,Duarte JA,Vitorino R,Neuparth MJ,Nunes C,Rocha C,Duarte I,Domingues MR

更新日期：2013-10-15 00:00:00

abstract：:The hnRNP A/B family contains abundant nuclear proteins with major roles in alternative splicing and the ability for nucleo-cytoplasmic shuttling. Compared to the best known members of this family (hnRNP A1, A2/B1), hnRNP A3 is a relatively less known protein. We report herein immunochemical studies with the hnRNP A3 ...

journal_title：Archives of biochemistry and biophysics

authors： Papadopoulou C,Boukakis G,Ganou V,Patrinou-Georgoula M,Guialis A

更新日期：2012-07-15 00:00:00

abstract：:As a means of comparing the functional properties of an enzyme in dilute solution in vitro with those for the same enzyme acting in its normal cellular environment, a study was conducted with 4-substituted pyrazoles as inhibitors of rat liver alcohol dehydrogenase in vitro and ethanol oxidation in isolated rat hepatoc...

journal_title：Archives of biochemistry and biophysics

authors： Cornell NW,Hansch C,Kim KH,Henegar K

更新日期：1983-11-01 00:00:00

abstract：:Maitotoxin (MTX) is a highly potent marine toxin that activates both voltage-sensitive and receptor-operated calcium channels in the plasma membrane. This results in calcium overload that rapidly leads to cell death. We now report that maitotoxin (0.1-1 nM) induces calpain activation in both SH-SY5Y neuroblastoma cell...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Nath R,Raser KJ,Hajimohammadreza I

更新日期：1996-07-15 00:00:00

abstract：:An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of inflammatory bowel diseases (IBD). Tumor necrosis alpha (TNFα) plays a central role in IBD pathogenesis, in part promoting tight function (TJ) barrier dysfunction. Food extracts enriched in (-)-epicatechin (EC) p...

journal_title：Archives of biochemistry and biophysics

authors： Contreras TC,Ricciardi E,Cremonini E,Oteiza PI

更新日期：2015-05-01 00:00:00

abstract：:This report describes the post-translational modifications of recombinant human differentiation-stimulating factor, a 180-residue glycoprotein that is secreted from transfected Chinese hamster ovary cells. Peptide peptides containing six potential N-glycosylation sites were analyzed to determine that Asn residues 9, 3...

journal_title：Archives of biochemistry and biophysics

authors： Schmelzer CH,Harris RJ,Butler D,Yedinak CM,Wagner KL,Burton LE

更新日期：1993-05-01 00:00:00