Redox properties of an engineered purple Cu(A) azurin.

abstract：:Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, stimulates both the cellular uptake of radiolabeled choline and the activity of CTP:phosphocholine cytidylyltransferase resulting in increased incorporation of choline into phosphatidylcholine (PtdCho). Because of these multiple actions, it...

journal_title：Archives of biochemistry and biophysics

authors： Kiss Z

更新日期：1996-11-01 00:00:00

abstract：:Nanomechanical properties of living cells, as measured with atomic force microscopy (AFM), are increasingly recognized as criteria that differentiate normal and pathologically altered cells. Locally measured cell elastic properties, described by the parameter known as Young's modulus, are currently proposed as a new d...

journal_title：Archives of biochemistry and biophysics

authors： Pogoda K,Pięta E,Roman M,Piergies N,Liberda D,Wróbel TP,Janmey PA,Paluszkiewicz C,Kwiatek WM

更新日期：2021-01-15 00:00:00

abstract：:The sensitivity of animals to endotoxin differs significantly between species. Thus, factors that determine the susceptibility to endotoxin may play important roles in the pathogenesis of septic shock. In order to determine the mechanism responsible for susceptibility to endotoxin, the effect of lipopolysaccharide (LP...

journal_title：Archives of biochemistry and biophysics

authors： Kida T,Kuroki T,Kobayashi K,Inoue M

更新日期：1994-07-01 00:00:00

abstract：:Recombinant ethanolamine ammonia-lyase from S. typhimurium has been overexpressed and purified in large quantities by a simple procedure. The molecular weight of the native enzyme is about 480 kDa, and it contains two active sites/molecule as shown by kinetic studies and by titration with CNCbl. Analysis by sodium dod...

journal_title：Archives of biochemistry and biophysics

authors： Faust LP,Babior BM

更新日期：1992-04-01 00:00:00

abstract：:Chlorite dismutase-like proteins are structurally closely related to functional chlorite dismutases which are heme b-dependent oxidoreductases capable of reducing chlorite to chloride with simultaneous production of dioxygen. Chlorite dismutase-like proteins are incapable of performing this reaction and their biologic...

journal_title：Archives of biochemistry and biophysics

authors： Hofbauer S,Hagmüller A,Schaffner I,Mlynek G,Krutzler M,Stadlmayr G,Pirker KF,Obinger C,Daims H,Djinović-Carugo K,Furtmüller PG

更新日期：2015-05-15 00:00:00

abstract：:Topoisomerase II is a major molecular target for a number of DNA-binding anticancer drugs. In the present study, we report topoisomerase II inhibition and anticancer activity by four substituted ferrocene derivatives which do not bind to DNA. The first derivative, acetyl-substituted ferrocene (monoacetylferrocene), sh...

journal_title：Archives of biochemistry and biophysics

authors： Vashisht Gopal YN,Jayaraju D,Kondapi AK

更新日期：2000-04-01 00:00:00

abstract：:The regulation of the plasma membrane Ca2+ pump by hormones via phosphorylation in intact cells has not been clearly established. We now present evidence that the Ca2+ pump is phosphorylated on both serine and threonine residues in unstimulated and stimulated cultured rat aortic endothelial cells. Among the stimuli te...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Du YS,Diglio C,Tsang W,Kuo TH

更新日期：1991-08-15 00:00:00

abstract：:The regulation of expression of hepatic iron-related proteins was examined during iron deficiency caused by scurvy in guinea pigs. Previous studies showed that some effects of scurvy, such as suppression of collagen gene expression, result from events associated with weight loss. During the initial phase of scurvy whe...

journal_title：Archives of biochemistry and biophysics

authors： Gosiewska A,Mahmoodian F,Peterkofsky B

更新日期：1996-01-15 00:00:00

abstract：:Cellular glutathione is released during apoptosis and may play a role in the regulation of the mitochondrial permeability transition pore. The question of whether only cytosolic glutathione is important in apoptosis, or whether mitochondrial glutathione also plays a role, was investigated using gamma-glutamyltranspept...

journal_title：Archives of biochemistry and biophysics

authors： Will Y,Kaetzel RS,Brown MK,Fraley TS,Reed DJ

更新日期：2002-01-15 00:00:00

abstract：:Much effort has focused on the identification of MAPK cascades that are activated by the MEKK family of protein kinases. However, direct phosphorylation and regulation of the MEKK proteins has not been shown. To address this question, we have expressed recombinant (His)6FLAG.MEKK3 in Sf9 insect cells and tethered the ...

journal_title：Archives of biochemistry and biophysics

authors： Adams DG,Sachs NA,Vaillancourt RR

更新日期：2002-11-01 00:00:00

abstract：:The hexapeptide Hyp-Gly-Pro-Lys-Gly-Glu was synthesized as a potential substrate for collagen lysyl hydroxylase. Kinetic data on the interaction of this peptide with purified chicken embryo lysyl hydroxylase showed that the hexapeptide is a moderately good substrate having Km, Vmax, and Kcat/Km values comparable to th...

journal_title：Archives of biochemistry and biophysics

authors： Ananthanarayanan VS,Saint-Jean A,Jiang P

更新日期：1992-10-01 00:00:00

abstract：:High-Mr and monomeric adenosine deaminase were injected intravenously into the rabbit. The rates of clearance and sites of uptake of the enzymes were compared. Calf intestinal mucosa served as the source of monomeric adenosine deaminase. High-Mr enzymes were assembled in vitro from the calf enzyme and adenosine deamin...

journal_title：Archives of biochemistry and biophysics

authors： Schrader WP,Harder CM,Schrader DK,West CA

更新日期：1984-04-01 00:00:00

abstract：:The dipeptidyl peptidase IV (DP IV, EC 3.4.14.5) gene from Flavobacterium meningosepticum was cloned by Southern and colony hybridizations using probes amplified by PCR, and expressed in Escherichia coli DH1. E. coli DH1 harboring pFDP-H1, which was a subclone derived from the positive clone pFDP-1, showed 3.5-fold hi...

journal_title：Archives of biochemistry and biophysics

authors： Kabashima T,Yoshida T,Ito K,Yoshimoto T

更新日期：1995-06-20 00:00:00

abstract：:We previously reported that rat testis and porcine testis contain a physicochemically indistinguishable Mr 32,000 calmodulin-binding protein, which we named "calspermin." In this study, antiserum against calspermin from porcine testis was raised in a rabbit, and a radioimmunoassay was developed. The antiserum reacted ...

journal_title：Archives of biochemistry and biophysics

authors： Ono T,Koide Y,Arai Y,Yamashita K

更新日期：1987-05-15 00:00:00

abstract：:The native conformation of bovine retinal arrestin has been characterized by a variety of spectroscopic methods. The purified protein gives rise to a near uv absorption band centered at 279 nm which results from the absorbance of its 14 tyrosine and one tryptophan residue. The extinction coefficient for this absorptio...

journal_title：Archives of biochemistry and biophysics

authors： Kotake S,Hey P,Mirmira RG,Copeland RA

更新日期：1991-02-15 00:00:00

abstract：:The manganese-containing (MnSOD) and iron-containing (FeSOD) superoxide dismutases from Escherichia coli are extensively (greater than 95%) inactivated by treatment with phenylglyoxal. The relatively high concentrations of phenylglyoxal and high pH required for optimal inactivation suggest that inactivation may be due...

journal_title：Archives of biochemistry and biophysics

authors： Borders CL Jr,Horton PJ,Beyer WF Jr

更新日期：1989-01-01 00:00:00

abstract：:Butyric acid produces multiple effects on mammalian cells in culture, including alterations in morphology, depression of growth rate, increased histone acetylation, and modified production of various proteins and enzymes. The latter effect is exemplified by the induction in HeLa cells of the glycoprotein hormone alpha...

journal_title：Archives of biochemistry and biophysics

authors： McClure DS,Cox GS

更新日期：1984-08-15 00:00:00

abstract：:A procedure was developed for the purification of an acetone-inducible form of cytochrome P-450 (P-450ac) to electrophoretical homogeneity from liver microsomes of acetone-treated rats. The P-450ac preparation containing 16.0 to 16.5 nmol P-450/mg protein moved as a single protein band with an estimated molecular weig...

journal_title：Archives of biochemistry and biophysics

authors： Patten CJ,Ning SM,Lu AY,Yang CS

更新日期：1986-12-01 00:00:00

abstract：:Activation of different forms of adenylate cyclases (AC) by forskolin and displacement of [14,15-3H]dihydroforskolin binding from membranes by forskolin in the absence or presence of specific stimulatory hormone and beta, gamma-imidoguanosine 5'-triphosphate (Gpp(NH)p) have been studied. These conditions have been use...

journal_title：Archives of biochemistry and biophysics

authors： Shi QH,Ruiz JA,Ho RJ

更新日期：1986-11-15 00:00:00

abstract：:The enhancement of the binding of plasma fibronectin to collagen or gelatin by heparin was previously thought to be due primarily to interaction of heparin with fibronectin. We observed, however, that the elution of purified human plasma fibronectin from heparin-treated gelatin-agarose required the same high urea conc...

journal_title：Archives of biochemistry and biophysics

authors： Smith RL,Griffin CA

更新日期：1988-10-01 00:00:00

abstract：:Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

journal_title：Archives of biochemistry and biophysics

authors： Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

更新日期：1984-06-01 00:00:00

abstract：:Maitotoxin (MTX) is a highly potent marine toxin that activates both voltage-sensitive and receptor-operated calcium channels in the plasma membrane. This results in calcium overload that rapidly leads to cell death. We now report that maitotoxin (0.1-1 nM) induces calpain activation in both SH-SY5Y neuroblastoma cell...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Nath R,Raser KJ,Hajimohammadreza I

更新日期：1996-07-15 00:00:00

abstract：:The extrahepatic expression of UDP-glucuronosyltransferases (UGTs) is important in the detoxification of a number of endogenous and exogenous compounds, including 5-hydroxytryptamine and morphine. Studies were designed to investigate the extrahepatic expression of human UGTs using RT-PCR techniques and to determine th...

journal_title：Archives of biochemistry and biophysics

authors： King CD,Rios GR,Assouline JA,Tephly TR

更新日期：1999-05-01 00:00:00

abstract：:Phorbol esters induce rapid downregulation of PAF receptors on human neutrophils. The molecular mechanism responsible for this observation has not been elucidated. In the present study, treatment of neutrophils with PMA (10(-7) M) for 10 min caused a complete loss of PAF receptors from the cell surface as determined b...

journal_title：Archives of biochemistry and biophysics

authors： Zhou W,Olson MS

更新日期：1994-08-15 00:00:00

abstract：:Alanine:glyoxylate aminotransferase has been reported to be present as the apo enzyme in the peroxisomes and as the holo enzyme in the mitochondria in chick (white leghorn) embryonic liver. However, surprisingly, birds were found to be classified into two groups on the basis of intraperoxisomal forms of liver alanine:...

journal_title：Archives of biochemistry and biophysics

authors： Sakuraba H,Fujiwara S,Noguchi T

更新日期：1991-05-01 00:00:00

abstract：:Apocynin has been used as an efficient inhibitor of the NADPH oxidase complex and its mechanism of inhibition is linked to prior activation through the action of peroxidases. Here we studied the oxidation of apocynin catalyzed by myeloperoxidase (MPO) and activated neutrophils. We found that apocynin is easily oxidize...

journal_title：Archives of biochemistry and biophysics

authors： Ximenes VF,Kanegae MP,Rissato SR,Galhiane MS

更新日期：2007-01-15 00:00:00

abstract：:The oxidized cytochrome c(2) from the purple phototrophic bacteria, Rhodobacter sphaeroides and Rhodobacter capsulatus, bind the neutral species of imidazole (K(a) = 1440 +/- 40 M(-1)) 50 times more strongly than does horse mitochondrial cytochrome c (K(a) = 30 +/- 1 M(-1)). The kinetics of imidazole binding are consi...

journal_title：Archives of biochemistry and biophysics

authors： Dumortier C,Meyer TE,Cusanovich MA

更新日期：1999-11-15 00:00:00

abstract：:We have studied the time-resolved fluorescence of three engineered Tet repressor (TetR) mutants bearing a single Trp residue at positions 162, 163, and 165 in the C-terminal part of the loop joining helices 8 and 9. Detailed analysis indicates that, at 20 degrees, the fluorescence decay of each Trp can be described as...

journal_title：Archives of biochemistry and biophysics

authors： Alberti P,Bombarda E,Kintrup M,Hillen W,Lami H,Piémont E,Doglia SM,Chabbert M

更新日期：1997-10-15 00:00:00

abstract：:We propose that adverse effects of the antibiotic metronidazole may be due, wholly or in part, to its conversion to a thiamine analog and consequent vitamin B1 antagonism. Consistent with this hypothesis, the drug is accepted as a substrate for the thiaminase (EC 2.5.1.2) elaborated as an exoenzyme by the human gut fl...

journal_title：Archives of biochemistry and biophysics

authors： Alston TA,Abeles RH

更新日期：1987-09-01 00:00:00

abstract：:The short term response of the L-6 cell line of rat skeletal myoblasts to elevated extracellular iron concentrations was studied. It was found in all cases that iron as the nitrilotriacetate (NTA) chelate was effective at donating iron to the cells and at stimulating ferritin synthesis. After 48 h in 50 microM ferric ...

journal_title：Archives of biochemistry and biophysics

authors： Rittling SR,Woodworth RC

更新日期：1983-08-01 00:00:00