Abstract:
:To evaluate the conformation of reduced HEL, the monoclonal antibodies HyC1 and HyC2, which recognize different conformational epitopes on native hen egg lysozyme (HEL), were used, and the kinetics of their interactions with native HEL, S-1,2-dicarboxyethylated HEL (DCE-HEL), and carboxymethylated Cys6 and Cys127 HEL (CM(6,127)-HEL) were assessed using surface plasmon resonance. Although their association rate constants differed 10(5)-fold, their dissociation rate constants were essentially the same, suggesting that DCE-HEL and CM(6,127)-HEL possess conformations similar to that of native HEL when they bind antibodies. We considered that the ratio of the association rate constant of reduced HEL to native HEL represents the proportion of the native format determinant in equilibrium. Reduction of the Cys6-Cys127 disulfide bond would transform the epitope recognized by HyC1 into a non-native conformation similar to that of DCE-HEL. We show that monoclonal antibodies provide a sensitive tool for evaluation of the structural and hydrodynamic changes of proteins.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Oda M,Kitai A,Murakami A,Nishimura M,Ohkuri T,Abe Y,Ueda T,Nakamura H,Azuma Tdoi
10.1016/j.abb.2009.11.024subject
Has Abstractpub_date
2010-02-15 00:00:00pages
145-50issue
2eissn
0003-9861issn
1096-0384pii
S0003-9861(09)00395-6journal_volume
494pub_type
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