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Elongation factor Ts of Chlamydia trachomatis: structure of the gene and properties of the protein.

Abstract：

:A putative structural gene cluster containing four open reading frames (ORFs) located downstream of the omp1 gene of Chlamydia trachomatis mouse pneumonitis (MoPn) was cloned and sequenced. A GenBank survey indicated that the identified cluster is similar to the rpsB-tsf-pyrH(smbA)-frr region of Escherichia coli. The second ORF was 846 bp encoding a 282-amino-acid polypeptide with a calculated M(r) 30,824. Alignment of this deduced protein sequence and E. coli elongation factor Ts (EF-Ts, product of tsf) demonstrated 34% identity and an additional 14% similarity. The putative chlamydial tsf gene was expressed in E. coli as a nonfusion protein and as a 6x His-tagged fusion protein. By SDS-PAGE analysis, the molecular weights of the nonfusion recombinant protein and a protein of chlamydial elementary bodies (EBs), which was recognized by monoclonal antibodies derived from the nonfusion recombinant protein, are 34 kDa. The purified recombinant 6x His-tagged fusion protein increased the rate of GDP exchange with both Chlamydia and E. coli elongation factor Tu (EF-Tu). These data show that the second gene of the identified cluster is tsf. Unlike EF-Ts from any other species, its activity was comparable to that of E. coli EF-Ts in exchange reaction with E. coli EF-Tu.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Zhang Y,Tao J,Zhou M,Meng Q,Zhang L,Shen L,Klein R,Miller DL

doi

10.1006/abbi.1997.0178

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

43-52

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003986197901788

journal_volume

344

pub_type

杂志文章

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