Molecular evolution and structure--function relationships of the superoxide dismutase gene families in angiosperms and their relationship to other eukaryotic and prokaryotic superoxide dismutases.

abstract：:The kinetics and thermodynamics of Ulex europeas agglutinin 1-H-antigenic determinant was studied by monitoring the binding of the lectin to H-antigenic fucolipid incorporated in liposomes tethered to the sensor surface by surface plasmon resonance. The second-order rate constants for the interactions are several orde...

journal_title：Archives of biochemistry and biophysics

authors： Thomas CJ,Surolia A

更新日期：2000-02-01 00:00:00

abstract：:Restriction fragments isolated from a 17-kb rat genomic DNA clone containing the gene for apolipoprotein (apo) E were radiolabeled and used to screen a rat liver cDNA library. A cDNA clone hybridizing to a 6-kb genomic DNA fragment was isolated and the nucleotide sequence of the cDNA insert determined. The sequence wa...

journal_title：Archives of biochemistry and biophysics

authors： Shen P,Howlett GJ

更新日期：1992-09-01 00:00:00

abstract：:Prothymosin alpha, a polypeptide of 109 to 111 amino acid residues, contains the entire thymosin alpha 1 sequence (residues 1-28) at its amino terminal. Human peripheral blood monocytes incubated with prothymosin alpha release thymosin alpha 1 in the culture supernatants. In addition total RNA is found to increase. Th...

journal_title：Archives of biochemistry and biophysics

authors： Frillingos S,Seferiadis K,Papanastasiou M,Baxevanis CN,Frangou-Lazaridis M,Economou M,Papamichail M,Tsolas O

更新日期：1992-07-01 00:00:00

abstract：:Using proton NMR, the solution conformation of the A ring of vitamin D3 and its analogs has been studied by application of the Karplus relation to the observed coupling constants. The A-ring conformations of vitamins D3, D2, and 25-hydroxyvitamin D3 were found to be solvent dependent, with a clear preference for an eq...

journal_title：Archives of biochemistry and biophysics

authors： Helmer B,Schnoes HK,DeLuca HF

更新日期：1985-09-01 00:00:00

abstract：:The portrait of osteoblasts and osteocytes has been subjected to a revision, since a large body of evidence is attributing these cells amazing roles both inside and outside the bone. The osteoblast, long confined to its bone building function, is actually a very eclectic cell, actively regulating osteoclast formation ...

journal_title：Archives of biochemistry and biophysics

authors： Capulli M,Paone R,Rucci N

更新日期：2014-11-01 00:00:00

abstract：:Flavin-dependent monooxygenases catalyze a wide variety of redox reactions in important biological processes and are responsible for the synthesis of highly complex natural products. Although much has been learned about FMO chemistry in the last ~80 years of research, several aspects of the reactions catalyzed by thes...

journal_title：Archives of biochemistry and biophysics

authors： Reis RAG,Li H,Johnson M,Sobrado P

更新日期：2021-01-16 00:00:00

abstract：:Tetrahydrobiopterin plays an important role in the biosynthesis of certain neurotransmitters. Using DEAE-Sepharose FF column chromatography, we separated the enzyme that synthesizes tetrahydrobiopterin from 6-pyruvoyl-tetrahydropterin [which is different from sepiapterin reductase (EC 1.1.1.153)] in the lemon mutant o...

journal_title：Archives of biochemistry and biophysics

authors： Iino T,Takikawa SI,Yamamoto T,Sawada H

更新日期：2000-01-15 00:00:00

abstract：:Calmodulin regulation of ATP-dependent Ca2+ transport activity was assessed in inverted basolateral plasma membrane vesicles (BLMV) isolated from rat parotid glands. The initial rate of Ca2+ transport in media containing 100 nM Ca2+ was stimulated by approximately 60% at maximal concentrations (300 nM) of exogenously ...

journal_title：Archives of biochemistry and biophysics

authors： Ambudkar IS,Horn VJ,Baum BJ

更新日期：1989-02-01 00:00:00

abstract：:Replication of the human immunodeficiency virus type 1 (HIV-1) is inhibited by interferons (IFNs), in part through activity of the IFN-inducible protein kinase PKR. To escape this antiviral effect, HIV-1 has developed strategies for blocking PKR function. We have previously shown that the HIV-1 Tat protein can associa...

journal_title：Archives of biochemistry and biophysics

authors： Cai R,Carpick B,Chun RF,Jeang KT,Williams BR

更新日期：2000-01-15 00:00:00

abstract：:Peroxynitrite-mediated one-electron oxidations may be an important event in its cytotoxic mechanisms, and yet, free radical formation in the presence of peroxynitrite is difficult to study by EPR-spin trapping because adducts from most spin traps are destroyed by the oxidant. This led to some controversy with regard t...

journal_title：Archives of biochemistry and biophysics

authors： Gatti RM,Alvarez B,Vasquez-Vivar J,Radi R,Augusto O

更新日期：1998-01-01 00:00:00

abstract：:The possibility of a cooperative interaction between fat-soluble antioxidants is examined in a membrane model. A combination of beta-carotene and alpha-tocopherol results in an inhibition of lipid peroxidation significantly greater than the sum of the individual inhibitions. Our data provide the first evidence that be...

journal_title：Archives of biochemistry and biophysics

authors： Palozza P,Krinsky NI

更新日期：1992-08-15 00:00:00

abstract：:Dpo4 is a representative model of Y-family DNA polymerase and is therefore one of the most intensively studied DNA polymerase. 6 mA, an epigenetic marker, plays important roles in regulation of various biological processes. However, its effects on DNA replication by Dpo4 is completely unknown. Here, we found that 6 mA...

journal_title：Archives of biochemistry and biophysics

authors： Du K,Zhang S,Chen W,Dai M,Xu Z,Liang T,Huang W,Ling Y,Zhang H

更新日期：2019-10-30 00:00:00

abstract：:Bovine kidney mitochondrial extracts contain an inactive protamine kinase and an inactive casein kinase. The protamine kinase was activated by chromatography on poly(L-lysine)-agarose. Two forms of this soluble mitochondrial protamine kinase were separated by chromatography on protamine-agarose. Both forms were purifi...

journal_title：Archives of biochemistry and biophysics

authors： Damuni Z,Reed LJ

更新日期：1988-05-01 00:00:00

abstract：:MAPK-activated protein kinase-2 (MAPKAPK2) regulates the synthesis of tumor necrosis factor and other cytokines and is a potential drug target for inflammatory diseases. Five protein constructs were produced in 4-10mg quantities per liter of culture media using baculovirus-infected insect cells and characterized for k...

journal_title：Archives of biochemistry and biophysics

authors： Kervinen J,Ma H,Bayoumy S,Schubert C,Milligan C,Lewandowski F,Moriarty K,Desjarlais RL,Ramachandren K,Wang H,Harris CA,Grasberger B,Todd M,Springer BA,Deckman I

更新日期：2006-05-15 00:00:00

abstract：:The partial degradation of proteoglycan aggregate by human leucocyte elastase yielded products that banded with Mr 190,000, 140,000, 88,000, and 71,000 when analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis. Analysis of these bands revealed that the 190,000- and 140,000-Da bands contained chondro...

journal_title：Archives of biochemistry and biophysics

authors： Mok MT,Ilic MZ,Handley CJ,Robinson HC

更新日期：1992-02-01 00:00:00

abstract：:Chronic kidney disease (CKD) is an increasingly recognized burden for patients and health care systems with high (and growing) global incidence and prevalence, significant mortality, and disproportionately high treatment costs. Yet, the available diagnostic tools are either impractical in clinical routine or have seri...

journal_title：Archives of biochemistry and biophysics

authors： Breit M,Weinberger KM

更新日期：2016-01-01 00:00:00

abstract：:Polyphyllin I (PPI), a bioactive constituent extracted from the rhizomes of Paris polyphylla, is cytotoxic to several cancer types. This study was designed to explore whether PPI prevents non-small-cell lung cancer (NSCLC) growth and to investigate the molecular mechanism. AMP-activated protein kinase (AMPK) has been ...

journal_title：Archives of biochemistry and biophysics

authors： Wu Y,Si Y,Xiang Y,Zhou T,Liu X,Wu M,Li W,Zhang T,Xiang K,Zhang L,Zhao H,Liu Y

更新日期：2020-07-15 00:00:00

abstract：:The presence of a cysteine residue at or near the active site of leukotriene A(4) hydrolase (EC 3.3.2.6) was suggested by inactivation of the enzyme with sulfhydryl-blocking reagents and by protection against inactivation afforded by substrates and competitive inhibitors. The aminopeptidase activity was more susceptib...

journal_title：Archives of biochemistry and biophysics

authors： Orning L,Fitzpatrick FA

更新日期：1999-08-01 00:00:00

abstract：:We have developed a protocol to purify apo-ovine (o) prostaglandin endoperoxide H(2) synthase-1 (PGHS-1) to homogeneity from ram seminal vesicles. The resulting apo enzyme can then be reconstituted with Co(3+)-protoporphyrin IX instead of Fe(3+)-protoporphyrin IX to produce a native-like, but functionally inert, enzym...

journal_title：Archives of biochemistry and biophysics

authors： Malkowski MG,Theisen MJ,Scharmen A,Garavito RM

更新日期：2000-08-01 00:00:00

abstract：:In spite of the abundance of antifungal therapies, 75% of women in the world suffer from the second most common cause of vaginal infection named vulvovaginal candidiasis. This complication is characterized with overgrowth of Candida albicans. The low efficacy and side effects of current antifungal therapies have convi...

journal_title：Archives of biochemistry and biophysics

authors： Ebrahimi-Shaghaghi F,Noormohammadi Z,Atyabi SM,Razzaghi-Abyaneh M

更新日期：2021-01-22 00:00:00

abstract：:A rabbit liver UDP-glucuronosyltransferase cDNA that is related to human and rat UGT1A7 has been identified. The predicted amino acid sequence of the UGT1A7l displays 80% similarity to that encoded by human HP4 (UGT1A9), but 81% to that predicted for human UGT1A7 and 77% to the rat UGT1A7 (UGTA2). The exons encoding h...

journal_title：Archives of biochemistry and biophysics

authors： Bruck M,Li Q,Lamb JG,Tukey RH

更新日期：1997-08-15 00:00:00

abstract：:Intensively treating type I diabetics with continuous subcutaneous insulin infusions or multiple daily insulin injections to normalize mean blood glucose concentrations significantly reduces the onset of secondary diabetic complications when compared to conventionally treated diabetics. Our studies focused on characte...

journal_title：Archives of biochemistry and biophysics

authors： Kramer KL,Giffin BF,Fox JW,Drake RL

更新日期：1999-08-15 00:00:00

abstract：:The soluble enzymatic luminescent system of the dinoflagellate Pyrocystis lunula (luciferase-luciferin) is coupled with an enzymatic NAD(P)H-dependent reaction. The enzyme is a soluble reductase (Mr 47,000) which catalyzes, in the presence of NAD(P)H, the reduction of a molecule called P630. Reduced P630 has the same ...

journal_title：Archives of biochemistry and biophysics

authors： Fresneau C,Hill M,Lescure N,Arrio B,Dupaix A,Volfin P

更新日期：1986-12-01 00:00:00

abstract：:Preparative isoelectric focusing and gel filtration chromatography were used to purify a carboxypeptidase produced by the entomopathogenic fungus Metarhizium anisopliae during growth on cockroach cuticle. The enzyme was inhibited by diisopropyl fluorophosphate, implying involvement of a serine residue in catalysis. Ho...

journal_title：Archives of biochemistry and biophysics

authors： St Leger RJ,Bidochka MJ,Roberts DW

更新日期：1994-11-01 00:00:00

abstract：:Comparative studies of 5-aminoimidazole ribonucleotide (AIR) carboxylases from Escherichia coli and Gallus gallus have identified this central step in de novo purine biosynthesis as a case for unusual divergence in primary metabolism. Recent discoveries establish the fungal AIR carboxylase, encoded by the ADE2 gene, a...

journal_title：Archives of biochemistry and biophysics

authors： Firestine SM,Misialek S,Toffaletti DL,Klem TJ,Perfect JR,Davisson VJ

更新日期：1998-03-01 00:00:00

abstract：:We evaluated the expression and activity of rat 12-lipoxygenase (LO) in rat intestinal epithelial (RIE) cells during apoptosis and cell differentiation. Sodium butyrate (NaBT) treatment induced wild-type RIE (W-RIE) cells to undergo differentiation and apoptosis. Alkaline phosphatase (ALP) activity, a marker of cell d...

journal_title：Archives of biochemistry and biophysics

authors： Kamitani H,Ikawa H,Hsi LC,Watanabe T,DuBois RN,Eling TE

更新日期：1999-08-01 00:00:00

abstract：:Subcutaneous implantation of demineralized bone matrix in rats induces migration of host cells into the site and results in the sequential development of cartilage and bone. The biosynthesis and metabolic fate of proteoglycans in the plaques at the bone matrix implantation site were investigated by [35S]sulfate labeli...

journal_title：Archives of biochemistry and biophysics

authors： Tian MY,Yanagishita M,Hascall VC,Reddi AH

更新日期：1986-05-15 00:00:00

abstract：:The glucose transporter of the bacterial phosphotransferase system mediates sugar transport across the cytoplasmic membrane concomitant with sugar phosphorylation. It consists of a cytoplasmic subunit IIA(Glc) and the transmembrane subunit IICB(Glc). IICB(Glc) was purified to homogeneity by urea/alkali washing of memb...

journal_title：Archives of biochemistry and biophysics

authors： Zhuang J,Gutknecht R,Flükiger K,Hasler L,Erni B,Engel A

更新日期：1999-12-01 00:00:00

abstract：:Arginase activity is elevated in livers of diabetic animals compared to controls and there is evidence that this is due in part to increased specific activity (activity/mg arginase protein). To investigate the molecular basis of this increased activity, the physicochemical and kinetic properties of hepatic arginase fr...

journal_title：Archives of biochemistry and biophysics

authors： Spolarics Z,Bond JS

更新日期：1989-11-01 00:00:00

abstract：:In a precedent report we showed that alpha-bisabolol, a sesquiterpene present widely in the plant kingdom, exerts a rapid and efficient apoptosis-inducing action selectively towards human and murine malignant glioblastoma cell lines through mitochondrial damage. The present study extends these data demonstrating the a...

journal_title：Archives of biochemistry and biophysics

authors： Darra E,Abdel-Azeim S,Manara A,Shoji K,Maréchal JD,Mariotto S,Cavalieri E,Perbellini L,Pizza C,Perahia D,Crimi M,Suzuki H

更新日期：2008-08-15 00:00:00