Abstract：
:In the present study, the activity of Topoisomerase IIβ (TopoIIβ) is evaluated during peroxide induced double stranded DNA breaks (DSBs) repair in primary neurons. The results showed that the TopoIIβ levels were enhanced during recovery from peroxide mediated damage (PED) along with Ku70, PARP-1, pol beta, and WRN helicase. Furthermore, siRNA mediated knock-down of TopoIIβ in primary neurons conferred enhanced susceptibility to PED in neurons. DSBs in neurons are repaired through two pathways, one promoted by Ku70, while the other is by PARP-1 dependent manner. Participation of TopoIIβ in both pathways was assessed by analysis of the interaction of TopoIIβ with Ku70 and PARP-1 using co-immunoprecipitation experiments in extracts of neurons under peroxide treatment and recovery. The results of these studies showed a strong interaction of TopoIIβ with Ku70 as well as PARP-1 suggesting that TopoIIβ is associated both in Ku70 and PARP-dependent pathways in DSBs repair in primary neurons. The study has thus established that TopoIIβ is an essential component in DSBs repair in primary neurons in both Ku70 and PARP-1 dependent pathways. We suppose that the interaction of TopoIIβ may provide stabilization of the repair complex, which may assist in maintenance of tensional integrity in genomic DNA.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Mandraju R,Chekuri A,Bhaskar C,Duning K,Kremerskothen J,Kondapi AKdoi
10.1016/j.abb.2011.10.001subject
Has Abstractpub_date
2011-12-15 00:00:00pages
128-37issue
2eissn
0003-9861issn
1096-0384pii
S0003-9861(11)00338-9journal_volume
516pub_type
杂志文章abstract：:Autophagy, a ubiquitous degradation pathway, is important for the survival and homeostasis of cells. Previous studies have demonstrated the role of autophagy in host defense against bacterial infection, but the importance of autophagy in the intestinal epithelium for the regulation of bacterial infection has not been ...
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pub_type： 杂志文章
doi：10.1006/abbi.1994.1312
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journal_title：Archives of biochemistry and biophysics
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doi：10.1016/0003-9861(90)90089-h
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journal_title：Archives of biochemistry and biophysics
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pub_type： 杂志文章
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abstract：:Cytochrome P4502E (P4502E), the major ethanol-inducible P450 metabolizes ethanol to acetaldehyde and bioactivates procarcinogens to ultimate carcinogens. Metabolism of ethanol to acetaldehyde in the brain could be deleterious since it can react with cytoskeletal proteins, forming adducts. In the present study, rats we...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1999.1477
更新日期：2000-01-01 00:00:00
