Antagonistic and agonistic effects of indigoids on the transformation of an aryl hydrocarbon receptor.

abstract：:DLIF (digoxin-like immunoreactive factor) and OLF (ouabain-like factor) are endogenous steroid-like ligands (approximately 781 and 595 Da, respectively) with molecular and structural properties similar to the plant-derived cardiac glycosides, digoxin and ouabain. We developed a purification method with a sufficiently ...

journal_title：Archives of biochemistry and biophysics

authors： Qazzaz HM,Valdes R Jr

更新日期：1996-04-01 00:00:00

abstract：:Atrial muscles of the heart are known to produce polypeptide hormones called atrial natriuretic factors (ANF) which have potent diuretic and hypotensive action. These hormones are synthesized as a larger protein precursor called pro atrial natriuretic factor or proANF which contains the biologically active ANF sequenc...

journal_title：Archives of biochemistry and biophysics

authors： Gierse JK,Olins PO,Devine CS,Marlay JD,Obukowicz MG,Mortensen LH,McMahon EG,Blaine EH,Seetharam R

更新日期：1989-06-01 00:00:00

abstract：:Sphingosine kinase 1 (SphK1) responds to a variety of growth factor signals by increasing catalytic activity as it translocates to the plasma membrane (PM). Several studies have identified amino acids residues involved in translocation yet how SphK1 increases its catalytic activity remains to be elucidated. Herein, we...

journal_title：Archives of biochemistry and biophysics

authors： Hengst JA,Guilford JM,Conroy EJ,Wang X,Yun JK

更新日期：2010-02-01 00:00:00

abstract：:A current hypothesis explaining the aging process implicates the accumulation of oxidized protein in animal tissues. This is primarily based on a series of reports showing an age-dependent increase in protein carbonyl content and an age-dependent decrease in the activities of enzymes, especially of alkaline proteases,...

journal_title：Archives of biochemistry and biophysics

authors： Cao G,Cutler RG

更新日期：1995-06-20 00:00:00

abstract：:The effects of fluorescein isothiocyanate II (FITC) on the actions of insulin in rat adipocytes were studied. When adipocytes were incubated with FITC at pH 7.4 (2 mM agent, 8 min), the cells were completely deprived of their specific insulin-binding activity and rendered unresponsive to the hormone. The effect of FIT...

journal_title：Archives of biochemistry and biophysics

authors： Goto Y,Sumida Y,Flanagan JE,Robinson FW,Simpson IA,Cushman SW,Kono T

更新日期：1992-03-01 00:00:00

abstract：:Sequence alignment of pig mitochondrial NADP-dependent isocitrate dehydrogenase with eukaryotic (human, rat, and yeast) and Escherichia coli isocitrate dehydrogenases reveals that Tyr316 is completely conserved and is equivalent to the E. coli Tyr345, which interacts with the 2'-phosphate of NADP in the crystal struct...

journal_title：Archives of biochemistry and biophysics

authors： Lee P,Colman RF

更新日期：2002-05-01 00:00:00

abstract：:The role of chalcone synthase in the regulation of flavonoid biosynthesis during organogenesis of oat primary leaves has been investigated at the level of enzyme activity and mRNA translation in vitro. Chalcone synthase was purified about 500-fold. The apparent Km values were 1.5 and 6.3 microM for 4-coumaroyl-CoA and...

journal_title：Archives of biochemistry and biophysics

authors： Knogge W,Schmelzer E,Weissenböck G

更新日期：1986-11-01 00:00:00

abstract：:Staphylococcus aureus is associated with several clinically significant infections among humans and infections associated with antibiotic-resistant strains are growing in frequency. Antivirulence strategies shift the target of drugs from bacterial growth to the infection process resulting to milder evolutionary pressu...

journal_title：Archives of biochemistry and biophysics

authors： Cueno ME,Imai K

更新日期：2018-05-01 00:00:00

abstract：:A lens epithelial cell line established from a transgenic mouse synthesizes high levels of the enzyme aldose reductase which converts sugars to polyols. This enzyme has been implicated in the formation of sugar cataracts in animals and with diabetic complications in man. The mouse aldose reductase has been characteriz...

journal_title：Archives of biochemistry and biophysics

authors： Russell P,Yamada T,Merola LO

更新日期：1990-01-01 00:00:00

abstract：:Cholesterol is an important lipid molecule and is needed for all mammalian cells. In various cell types, excess cholesterol is stored as cholesteryl esters; acyl-CoA:cholesterol acyltransferase 1 (ACAT1) plays an essential role in this storage process. ACAT1 is located at the endoplasmic reticulum and has nine transme...

journal_title：Archives of biochemistry and biophysics

authors： Neumann B,Chang CCY,Chang TY

更新日期：2019-08-15 00:00:00

abstract：:X-ray crystallographic studies on glucose oxidase showed a strong interaction between carbohydrate and protein moieties of the glycoprotein. However, experimental studies under physiological conditions reported no influence of carbohydrate moiety on the structural and functional properties of glucose oxidase. In order...

journal_title：Archives of biochemistry and biophysics

authors： Akhtar MS,Bhakuni V

更新日期：2003-05-15 00:00:00

abstract：:We have studied the effectiveness of polyprenyl-P-mannose and polyprenol-P-glucose as donor substrates for the dolichyl-P-mannose:Man5(GlcNAc)2-PP-dolichol mannosyltransferase and the dolichyl-P-glucose:Man9(GlcNAc)2-PP-dolichol glucosyltransferase, respectively. The polyprenol moiety differs from dolichol only in the...

journal_title：Archives of biochemistry and biophysics

authors： D'Souza-Schorey C,McLachlan KR,Krag SS,Elbein AD

更新日期：1994-02-01 00:00:00

abstract：:An in vitro assay that measures endosome fusion was used to characterize the role of guanosine triphosphate (GTP)-binding proteins in endocytosis. Guanosine 5',3-(thio)triphosphate (GTP gamma S), a nonhydrolyzable analog of GTP, stimulates the binding of cytosolic factors to the endosomal membrane (priming). GTP gamma...

journal_title：Archives of biochemistry and biophysics

authors： Lenhard JM,Colombo MI,Stahl PD

更新日期：1994-08-01 00:00:00

abstract：:Physicochemical properties and catalytic activity of cytochrome P4501A2 in the reconstituted system with Emulgen 913 were studied. The formation of cytochrome P4501A2 monomers was shown by gel filtration at an Emulgen concentration of 8 g/liter. The catalytic activity of the monomeric monooxygenase system was low. The...

journal_title：Archives of biochemistry and biophysics

authors： Sevrukova IF,Kanaeva IP,Koen YM,Samenkova NF,Bachmanova GI,Archakov AI

更新日期：1994-05-15 00:00:00

abstract：:Methyl acetyl phosphate, which was originally synthesized as a site-specific reagent for hydroxybutyrate dehydrogenase [R. Kluger and W.-C. Tsui (1980) J. Org. Chem. 45, 2723], also has an affinity for the binding site for 2,3-diphosphoglycerate in hemoglobin. Three residues in or near this cleft between the beta-chai...

journal_title：Archives of biochemistry and biophysics

authors： Ueno H,Pospischil MA,Manning JM,Kluger R

更新日期：1986-02-01 00:00:00

abstract：:The effect of the nemaline myopathy-causing E117K mutation in β-tropomyosin (TM) on the structure and function of this regulatory protein was studied. The E117K mutant was found to have indistinguishable actin affinity compared with wild-type (WT) and similar secondary structure as measured by circular dichroism. Howe...

journal_title：Archives of biochemistry and biophysics

authors： Karpicheva OE,Robinson P,Piers A,Borovikov YS,Redwood CS

更新日期：2013-08-01 00:00:00

abstract：:Gene expression in the obligate intracellular bacterium Chlamydia trachomatis ranges from nil in the infectious EB form to high in the dividing RB form. Little is known about the mechanisms of gene regulation in chlamydiae and only a few promoter sequences have been characterized. The purpose of our study was to exami...

journal_title：Archives of biochemistry and biophysics

authors： Shen L,Shi Y,Douglas AL,Hatch TP,O'Connell CM,Chen JM,Zhang YX

更新日期：2000-07-01 00:00:00

abstract：:A solution hybridization assay employing specific synthetic oligodeoxynucleotide probes was developed to study the regulation of intestinal calbindin-D28K mRNA. The technique is rapid and quantitative and eliminates the need for sample transfer, blotting, autoradiography, and densitometry. Following validation of the ...

journal_title：Archives of biochemistry and biophysics

authors： Fullmer CS

更新日期：1990-11-15 00:00:00

abstract：:An improved high yielding procedure for the purification of myrosinase from Sinapis alba L. consisting of concanavalin A affinity chromatography followed by a chromatofocusing step is presented. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate-gel electrophoresis and by analytical ultracentrifug...

journal_title：Archives of biochemistry and biophysics

authors： Pessina A,Thomas RM,Palmieri S,Luisi PL

更新日期：1990-08-01 00:00:00

abstract：:Nanomechanical properties of living cells, as measured with atomic force microscopy (AFM), are increasingly recognized as criteria that differentiate normal and pathologically altered cells. Locally measured cell elastic properties, described by the parameter known as Young's modulus, are currently proposed as a new d...

journal_title：Archives of biochemistry and biophysics

authors： Pogoda K,Pięta E,Roman M,Piergies N,Liberda D,Wróbel TP,Janmey PA,Paluszkiewicz C,Kwiatek WM

更新日期：2021-01-15 00:00:00

abstract：:The kinetic effects of a selection of triarylmethane, phenoxazine and phenothiazine dyes (pararosaniline (PR), malachite green (MG), methyl green (MeG); meldola blue (MB), nile blue (NB), nile red (NR); methylene blue (MethB)) and of ethopropazine on horse serum butyrylcholinesterase were studied spectrophotometricall...

journal_title：Archives of biochemistry and biophysics

authors： Yücel YY,Tacal O,Ozer I

更新日期：2008-10-15 00:00:00

abstract：:The biosynthesis of one riboflavin molecule requires one molecule of GTP and two molecules of ribulose 5-phosphate as substrates. GTP is hydrolytically opened, converted into 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione by a sequence of deamination, side chain reduction and dephosphorylation. Condensation with 3,...

journal_title：Archives of biochemistry and biophysics

authors： Fischer M,Bacher A

更新日期：2008-06-15 00:00:00

abstract：:A rabbit liver UDP-glucuronosyltransferase cDNA that is related to human and rat UGT1A7 has been identified. The predicted amino acid sequence of the UGT1A7l displays 80% similarity to that encoded by human HP4 (UGT1A9), but 81% to that predicted for human UGT1A7 and 77% to the rat UGT1A7 (UGTA2). The exons encoding h...

journal_title：Archives of biochemistry and biophysics

authors： Bruck M,Li Q,Lamb JG,Tukey RH

更新日期：1997-08-15 00:00:00

abstract：:The topographical localization of the N-terminus of cytochrome b in the inner mitochondrial membrane was determined by mild proteolysis of the yeast mitochondrial cytochrome bc1 complex and identification of the proteolytic fragments derived from subunits of the complex with an established orientation in the inner mem...

journal_title：Archives of biochemistry and biophysics

authors： Beattie DS,Jenkins HC,Howton MM

更新日期：1994-07-01 00:00:00

abstract：:Triacylglycerol plays a critical role in an organism's ability to withstand fuel deprivation, and dysregulation of triacylglycerol synthesis is important in the development of diseases such as obesity and diabetes. Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial and committed step of glycerolipid syn...

journal_title：Archives of biochemistry and biophysics

authors： Lewin TM,Granger DA,Kim JH,Coleman RA

更新日期：2001-12-01 00:00:00

abstract：:The hepatic cytosolic hemoprotein tryptophan 2,3-dioxygenase (TDO) is the rate-limiting enzyme in tryptophan catabolism and thus plays a key role in regulating the physiological flux of tryptophan into relevant metabolic pathways. The TDO protein is induced by corticosteroids such as dexamethasone (DEX) and is stabili...

journal_title：Archives of biochemistry and biophysics

authors： Ren S,Correia MA

更新日期：2000-05-01 00:00:00

abstract：:Escherichia coli pyruvate oxidase (POXEC) requires FAD both for the oxidative decarboxylation of pyruvate to acetate and CO2 and for the formation of acetoin from pyruvate and acetaldehyde. Prior work has shown that the catalytic activity (kcat/Km) for POXEC in the oxidative reaction is stimulated approximately 450-fo...

journal_title：Archives of biochemistry and biophysics

authors： Bertagnolli BL,Hager LP

更新日期：1993-01-01 00:00:00

abstract：:Proteins lacking prosthetic groups and/or cofactors are known to undergo electronic excitation transitions only upon exposure to UV-C (< 280 nm) and UV-B (280-320 nm), but not UV-A (320-400 nm) photons. Here, we report the discovery of a novel excitation that peaks at approximately 340 nm and yields visible violet-blu...

journal_title：Archives of biochemistry and biophysics

authors： Shukla A,Mukherjee S,Sharma S,Agrawal V,Radha Kishan KV,Guptasarma P

更新日期：2004-08-15 00:00:00

abstract：:Microsomal P450s catalyze the monooxygenation of a large variety of hydrophobic compounds, including drugs, steroids, carcinogens, and fatty acids. The interaction of microsomal P450s with their electron transfer partner, NADPH-P450 reductase, during the transfer of electrons from NADPH to P450, for oxygen activation,...

journal_title：Archives of biochemistry and biophysics

authors： Peterson JA,Boddupalli SS

更新日期：1992-05-01 00:00:00

abstract：:This paper describes the effect of an ionic (sodium dodecyl sulfate; SDS) and a nonionic detergent (Triton X-100) on the substrate and enzyme components of the lecithin: cholesterol acyltransferase (LCAT) reaction. When the enzyme sources (purified or partially purified) or the respective substrates [high-density lipo...

journal_title：Archives of biochemistry and biophysics

authors： Park MC,McConathy WJ,Lacko AG

更新日期：1984-08-15 00:00:00