Biochemical characterization of temperature-induced changes in lipid metabolism in a high oleic acid mutant of Brassica rapa.

abstract：:Mutagenic substitution of the invariant D42 and D169 residues in phosphoribulokinase (PRK) with amino acids that contain neutral side chains (e.g., alanine or asparagine) results in large decreases in catalytic efficiency (10(5)- and 10(4)-fold for replacement of D42 and D169, respectively). To further evaluate the im...

journal_title：Archives of biochemistry and biophysics

authors： Runquist JA,Miziorko HM

更新日期：2002-09-15 00:00:00

abstract：:Vascular smooth muscle cell (VSMC) proliferation is a key event in the progression of atherosclerosis. Induction of both c-fos (through the transcription factor Elk-1) and c-jun, both immediate early genes, is important for the stimulation of VSMC proliferation and migration. It was earlier found that p38 mitogen-acti...

journal_title：Archives of biochemistry and biophysics

authors： Zhao M,Liu Y,Bao M,Kato Y,Han J,Eaton JW

更新日期：2002-04-15 00:00:00

abstract：:The activity of thymidylate synthetase in the liver of the ddY strain male mouse increased transitorily according to the increase in tumor cell number at maximum 7-9 days after ip transplantation of Ehrlich ascites tumor. The enzyme was able to be purified from the tumor host mouse liver or from the normal mouse liver...

journal_title：Archives of biochemistry and biophysics

authors： Asanagi M,Moriyama Y,Fujimura S

更新日期：1988-12-01 00:00:00

abstract：:Fibronectin heterogeneity is, in part, the result of post-translational modifications. In these experiments, cartilage fibronectins were purified by anion exchange chromatography, followed by gelatin affinity chromatography or immunoprecipitation, and, finally, sodium dodecyl sulfate--polyacrylamide gel electrophoresi...

journal_title：Archives of biochemistry and biophysics

authors： Burton-Wurster N,Lust G

更新日期：1993-11-01 00:00:00

abstract：:The dicarbonyl compounds, phenylgloxyl and 2,3-butanedione were used to demonstrate the presence of an essential arginine residue in the mechanism of the red beet (Beta vulgaris L.) plasma membrane ATPase. Treatment of the red beet ATPase with either of these reagents resulted in an inhibition of ATP hydrolytic activi...

journal_title：Archives of biochemistry and biophysics

authors： Gildensoph LH,Briskin DP

更新日期：1989-05-15 00:00:00

abstract：:Recently, we synthesized and characterized vanadyl saccharides to evaluate the effects of various vanadate and vanadyl complexes, which differ in their oxidation states on various biomacromolecules and cellular activities (1, 2). Here, we report that both vanadate (+V oxidation state) and different vanadyl species (+I...

journal_title：Archives of biochemistry and biophysics

authors： Krishnamoorthy T,Sreedhara A,Rao CP,Ramaiah KV

更新日期：1998-01-01 00:00:00

abstract：:A 1.1-kb Hp alpha I fragment of the Escherichia coli chromosome containing the gene for translation initiation factor 3 was employed as a probe in heterologous hybridization to chromosomal DNA from a variety of other procaryotes. Positive hybridization was observed to DNA derived from all gram-negative bacteria tested...

journal_title：Archives of biochemistry and biophysics

authors： Pramanik A,Schwartz I

更新日期：1984-11-15 00:00:00

abstract：:Polycation binding to the negatively charged surface of chloroplast thylakoid membranes is known to cause an inhibition of photosystem I activity. It also interferes with the cation-dependent rearrangement of chlorophyll proteins in the thylakoid membrane. It was shown that added anions prevented or reversed the inhib...

journal_title：Archives of biochemistry and biophysics

authors： Richter ML,Homann PH

更新日期：1983-04-01 00:00:00

abstract：:Homogeneous cultures of epithelial, endothelial, and mesangial cells from calf glomeruli were radiolabeled with [35S]sulfate in order to evaluate their capacity for the biosynthesis of the proteoglycan (PG) components present in the glomerular extracellular matrix. Although each cell type was observed to incorporate i...

journal_title：Archives of biochemistry and biophysics

authors： Shen GQ,Kresbach G,Spiro MJ,Spiro RG

更新日期：1995-08-01 00:00:00

abstract：:In vitro phosphorylation of purified spectrin dimer was studied in the presence of Ca2+-calmodulin (CaM). CaM inhibited autophosphorylation of the beta subunit of spectrin. The inhibitory effect (65% at a 32-fold molar excess) appeared to be due to a weak interaction of CaM with spectrin. CaM was similarly effective i...

journal_title：Archives of biochemistry and biophysics

authors： Maretzki D,Lutz HU

更新日期：1986-04-01 00:00:00

abstract：:The pH gradient, delta pH, present across the inner mitochondrial membrane in isolated rat hepatocytes was continuously monitored with a novel spectroscopic technique that utilizes the weak acid fluorescein. Unlike most cytosolic pH indicators, such as 2',7'-bis(carboxyethyl)-5,(6)-carboxyfluorescein (BCECF), fluoresc...

journal_title：Archives of biochemistry and biophysics

authors： Thomas PJ,Gaspers LD,Pharr C,Thomas JA

更新日期：1991-07-01 00:00:00

abstract：:In addition to the broad repertoire of regulatory functions nitric oxide (NO) serves in mammalian physiology, the L-arginine:NO pathway is also involved in numerous pathophysiological mechanisms. While NO itself may actually protect cells from the toxicity of reactive oxygen radicals in some cases, it has been suggest...

journal_title：Archives of biochemistry and biophysics

authors： Wink DA,Feelisch M,Fukuto J,Chistodoulou D,Jourd'heuil D,Grisham MB,Vodovotz Y,Cook JA,Krishna M,DeGraff WG,Kim S,Gamson J,Mitchell JB

更新日期：1998-03-01 00:00:00

abstract：:The ferric and ferrous forms of bovine erythrocyte green hemeprotein react with hydroperoxides to form higher oxidation state intermediates with absorbance maxima in the Soret region at 426 and 422 nm, respectively. In the absence of an appropriate reductant, these intermediates undergo rapid bleaching reactions. 2,2'...

journal_title：Archives of biochemistry and biophysics

authors： Xu F,DeFilippi LJ,Ballou DP,Hultquist DE

更新日期：1993-02-15 00:00:00

abstract：:By exploiting its capacity for binding to DNA, the protease inhibitor alpha 1-antichymotrypsin has been isolated from human serum by ammonium sulfate fractionation and successive chromatography on QAE-Sephadex, DNA-cellulose, and Sephacryl S-300. This experimental procedure compares favorably with existing methods for...

journal_title：Archives of biochemistry and biophysics

authors： Abdullah M,Siddiqui AA,Hill JA,Davies RJ

更新日期：1983-08-01 00:00:00

abstract：:Site-mutations were introduced into putative cation binding site 1 of the H,K-ATPase at glu-797, thr-825, and glu-938. The side chain oxygen of each was not essential but the mutations produced different activation and inhibition kinetics. Site mutations thr-825 (ala, leu) and glu-938 (ala, gln) modestly decreased the...

journal_title：Archives of biochemistry and biophysics

authors： Rulli SJ,Louneva NM,Skripnikova EV,Rabon EC

更新日期：2001-03-01 00:00:00

abstract：:P-815 mouse mastocytoma cells express the K isozyme of pyruvate kinase and the specific activity of this enzyme is increased in response to N6,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate, 8-bromoadenosine 3':5'-cyclic monophosphate, cholera toxin, and epinephrine, all of which also elevate the intracellular con...

journal_title：Archives of biochemistry and biophysics

authors： Ngo JL,Ibsen KH

更新日期：1989-03-01 00:00:00

abstract：:During the deglycosylation reaction of fish egg polysialoglycoproteins under the conditions of 1 M NaBH4 in 0.1 M NaOH at 37 degrees C for 48 h, a marked loss of the glycine content has been encountered, besides the serine and threonine residues to which the carbohydrate units are linked. The chemical basis behind thi...

journal_title：Archives of biochemistry and biophysics

authors： Shimamura M,Inoue Y,Inoue S

更新日期：1984-08-01 00:00:00

abstract：:Glucose-6-phosphatase is a multicomponent system located in the endoplasmic reticulum, involving both a catalytic subunit (G6PC) and several substrate and product carriers. The glucose-6-phosphate carrier is called G6PT1. Using light scattering, we determined K(D) values for phosphate and glucose transport in rat live...

journal_title：Archives of biochemistry and biophysics

authors： Clottes E,Middleditch C,Burchell A

更新日期：2002-12-01 00:00:00

abstract：:The state of the various, spectroscopically distinguishable copper sites (type 1, type 2, and type 3 copper) of human ceruloplasmin was investigated by electron spin resonance (ESR) spectroscopy. The ESR measurements were performed at 100 K and at X-band during the reaction of the protein with either ascorbate or with...

journal_title：Archives of biochemistry and biophysics

authors： Musci G,Bonaccorsi di Patti MC,Calabrese L

更新日期：1993-10-01 00:00:00

abstract：:The effects of extracellular ATP on phosphoinositide metabolism and intracellular Ca2+ homeostasis were studied in Ehrlich ascites tumor cells. Cytosolic [Ca2+] was measured using either quin 2 or the recently described indicator fura 2. Addition of 0.5-25 microM extracellular ATP to intact cells results in a rapid mo...

journal_title：Archives of biochemistry and biophysics

authors： Dubyak GR

更新日期：1986-02-15 00:00:00

abstract：:The formation of alpha-hydroxyethyl radical from ethanol by deermouse microsomes supplemented with NADPH has been demonstrated with the EPR technique of spin trapping with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN) as the spin trap, in the presence of deferoxamine mesylate. Superoxide dismutase prevented the fo...

journal_title：Archives of biochemistry and biophysics

authors： Knecht KT,Thurman RG,Mason RP

更新日期：1993-06-01 00:00:00

abstract：:Bovine pancreatic trypsin inhibitor (BPTI) and tick anticoagulant peptide (TAP) are two structurally homologous proteins, which have been shown to exhibit distinct mechanisms of oxidative folding. We demonstrate here differences of their folding properties using the technique of disulfide scrambling. Two extensively u...

journal_title：Archives of biochemistry and biophysics

authors： Chang JY,Li L

更新日期：2005-05-01 00:00:00

abstract：:Alkaline phosphatase (AP) was purified to over 90% homogeneity from rat osteosarcoma by acetone precipitation followed by chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxyapatite. The purified enzyme had a specific activity of 759 units/mg protein at its optimal pH (10.5), and a Km of 0.8 mM for p-nitroph...

journal_title：Archives of biochemistry and biophysics

authors： Nair BC,Majeska RJ,Rodan GA

更新日期：1987-04-01 00:00:00

abstract：:Nucleotide-free kinesin motor domains from several kinesin families convert reversibly to a refractory conformation that cannot rapidly rebind ADP. In the absence of glycerol, the refractory conformation of Drosophila kinesin motor domains is favored by 50-fold with conversion of the active to the refractory species a...

journal_title：Archives of biochemistry and biophysics

authors： Hackney DD,McGoff MS

更新日期：2016-10-15 00:00:00

abstract：:Cardiac hypertrophy is an adaptive response to hemodynamic stress to compensate for cardiac dysfunction. MicroRNAs can regulate cardiac function and play a vital role in the regulation of cardiac hypertrophy. In the current study, in vivo and vitro hypertrophy models are established to explore the role of miR-27b and ...

journal_title：Archives of biochemistry and biophysics

authors： Zhang M,Cheng K,Chen H,Tu J,Shen Y,Pang L,Wu W

更新日期：2020-08-15 00:00:00

abstract：:The essential Zn(II) in bovine liver dihydropyrimidine amidohydrolase (DHPase) was removed by incubation with 2,6-dipicolinic acid and replaced with Mn(II). Electron paramagnetic resonance studies of Mn(II) binding show that there are four binding sites per tetramer, and the dissociation constant at pH 7.5 is 13.5 mic...

journal_title：Archives of biochemistry and biophysics

authors： Lee MH,Pettigrew DW,Sander EG,Nowak T

更新日期：1987-12-01 00:00:00

abstract：:New and stimulating results have challenged the concept that cellular senescence might not be synonymous with aging. It is indisputable that during aging, senescent cell accumulation has an impact on organismal health. Nevertheless, senescent cells are now known to display physiological roles during embryonic developm...

journal_title：Archives of biochemistry and biophysics

authors： Triana-Martínez F,Pedraza-Vázquez G,Maciel-Barón LA,Königsberg M

更新日期：2016-05-15 00:00:00

abstract：:A cyanide-insensitive superoxide dismutase was purified to electrophoretic homogeneity from leaves of the eucaryote, Ginkgo biloba L. A molecular mass of 47,000 was determined for the enzyme, which consisted of two subunits of equal size. The enzyme preparation contained two isoenzymes with isoelectric points of 5.25 ...

journal_title：Archives of biochemistry and biophysics

authors： Duke MV,Salin ML

更新日期：1985-11-15 00:00:00

abstract：:A microsomal retinol dehydrogenase (RoDH) that recognizes holo-cellular retinol binding protein (CRBP) as substrate is inhibited by phenylarsine oxide (IC50 = 3 microM) in the presence of 10 mM cysteine. Inhibition was reversible with dithiothreitol, indicating that two cysteine residues in close proximity are essenti...

journal_title：Archives of biochemistry and biophysics

authors： Boerman MH,Napoli JL

更新日期：1995-08-20 00:00:00

abstract：:The first committed step in prostaglandin biosynthesis is catalyzed by prostaglandin H synthase, an enzyme localized to the endoplasmic reticulum (ER) membrane in a variety of cells. Several types of C-terminal region peptide sequence motifs have been found to lead to ER retention of other proteins. We have tested the...

journal_title：Archives of biochemistry and biophysics

authors： Ren Y,Loose-Mitchell DS,Kulmacz RJ

更新日期：1995-02-01 00:00:00