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Effects of sulfhydryl reagents, retinoids, and solubilization on the activity of microsomal retinol dehydrogenase.

Abstract：

:A microsomal retinol dehydrogenase (RoDH) that recognizes holo-cellular retinol binding protein (CRBP) as substrate is inhibited by phenylarsine oxide (IC50 = 3 microM) in the presence of 10 mM cysteine. Inhibition was reversible with dithiothreitol, indicating that two cysteine residues in close proximity are essential for RoDH activity. Bromophenylarsine oxide was an irreversible inhibitor (IC50 = 0.2 microM), suggesting that a nucleophile lies close to the two cysteine residues. N-Ethylmaleimide inhibited reactions supported by holo-CRBP, but not from free retinol, suggesting that it obstructed holo-CRBP access to RoDH without affecting the catalytic site. RoDH activity was similar in microsomes from vitamin A-sufficient or vitamin A-deficient rats and was not inhibited by relatively high concentrations (5 microM) of all-trans-retinoic acid, holo-cellular retinoic acid binding protein, apo-cellular retinoic acid binding protein, or 9-cis-retinoic acid. Triton X-100 stimulated RoDH activity eightfold at a detergent to protein ratio of 0.25 to 1 (w/w). A combination of Tween 80, Brij 92, and Triton X-100 (2:1:2) stimulated RoDH activity eightfold at a detergent to protein ratio of 2.5 to 1 (w/w). Detergent-solubilized RoDH, partially purified through a PAO-Sepharose resin, preferred NADP(H) as cofactor, had a Km for retinal synthesis from holo-CRBP of 0.6 microM (Vmax = 115 pmol/min/mg protein) and a Km for reduction of retinal bound to CRBP of 0.6 microM (Vmax = 613 pmol/min/mg protein). This work provides further insight into microsomal RoDH and strengthens the evidence of an interaction between RoDH and holo-CRBP.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Boerman MH,Napoli JL

doi

10.1006/abbi.1995.1415

subject

Has Abstract

pub_date

1995-08-20 00:00:00

pages

434-41

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(85)71415-4

journal_volume

321

pub_type

杂志文章

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