p53 regulates Hsp90beta during arsenite-induced cytotoxicity in glutathione-deficient cells.


:p53, a tumor suppressor and transcription factor, is a critical modulator in the cellular response to stress. Exposure of glutathione-deficient GCS-2 cells to arsenite significantly phosphorylated and stabilized p53. In addition, p53 transcriptionally repressed Hsp90beta gene expression. Mutation analysis revealed a p53 binding site in the 5' flanking region responsible for the regulation of Hsp90beta gene. Electrophoretic mobility shift assay showed that p53 is bound to Hsp90beta promoter region. ATM kinase, a major determinant in the modulation of p53 specifically affected its phosphorylation at Ser-15. ATM kinase-mediated phosphorylation of p53 is regulated through phosphorylation of Chk2. Down-regulation of ATM and Chk2 by their small interfering RNAs (siRNAs) attenuated the arsenite-induced phosphorylation of p53 and restored Hsp90beta mRNA levels. Taken together, these findings suggest that arsenite acts through ATM and Chk2 to induce phosphorylation of p53. This results in the transcriptional repression of Hsp90beta, under GSH-deficient conditions which may play a role in arsenic-mediated pathogenesis.


Arch Biochem Biophys


Habib GM




Has Abstract


2009-01-01 00:00:00














  • Docking of verapamil in a synthetic Ca2+ channel: formation of a ternary complex involving Ca2+ ions.

    abstract::The mechanism by which diverse drugs modulate voltage-dependent Ca2+ channels is ill-understood. We have approached this problem by examining the interaction of verapamil with a 97-residue synthetic channel peptide (SCP) that exhibits functional similarities to authentic L-type Ca2+ channels in terms of cation selecti...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhorov BS,Ananthanarayanan VS

    更新日期:1997-05-15 00:00:00

  • Expression of the Chlamydomonas reinhardtii chloroplast tRNA(Glu) gene in a homologous in vitro transcription system is independent of upstream promoter elements.

    abstract::Chloroplast tRNA(Glu) is a bifunctional molecule involved in both the early steps of chlorophyll synthesis and chloroplast protein biosynthesis. Recently the enzymes involved in these processes have been characterized from the green alga Chlamydomonas reinhardtii. In order to investigate whether transcription of the g...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jahn D

    更新日期:1992-11-01 00:00:00

  • Interflavin one-electron transfer in the inducible nitric oxide synthase reductase domain and NADPH-cytochrome P450 reductase.

    abstract::In this study, we have analyzed interflavin electron transfer reactions from FAD to FMN in both the full-length inducible nitric oxide synthase (iNOS) and its reductase domain. Comparison is made with the interflavin electron transfer in NADPH-cytochrome P450 reductase (CPR). For the analysis of interflavin electron t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Yamamoto K,Kimura S,Shiro Y,Iyanagi T

    更新日期:2005-08-01 00:00:00

  • Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells.

    abstract::The objective of this study was to investigate the antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A (ConA) on human melanoma A375 cells. We firstly simulated the three-dimensional structure of ConA. Subsequently, we found that ConA possessed remarkable antiproliferative effect on A375 cell...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liu B,Li CY,Bian HJ,Min MW,Chen LF,Bao JK

    更新日期:2009-02-01 00:00:00

  • Electrostatically mediated interactions between cationic lipid-DNA particles and an anionic surface.

    abstract::In an effort to model the interaction of lipid-based DNA delivery systems with anionic surfaces, such as a cell membrane, we have utilized microelectrophoresis to characterize how electrokinetic measurements can provide information on surface charge and binding characteristics. We have established that cationic lipids...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wong FM,Bally MB,Brooks DE

    更新日期:1999-06-01 00:00:00

  • Characterization of active and inactive forms of rat hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase.

    abstract::Rat hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was purified to homogeneity using agarose-HMG-CoA affinity chromatography. Additional protein was isolated from the affinity column with 0.5 M KCl that demonstrated no HMG-CoA reductase activity, yet comigrated with purified HMG-CoA reductase on sod...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Whitehead TR,Vlahcevic ZR,Beg ZH,Hylemon PB

    更新日期:1984-05-01 00:00:00

  • Expression and function of three cytochrome P-450 isozymes in rat extrahepatic tissues.

    abstract::Western blots using a polyclonal and a monoclonal antibody raised against rat liver cytochrome P-450b indicate tissue-specific expression of low levels of cytochrome P-450's b and e. P-450b and P-450e were expressed very selectively in, respectively, lung and adrenal microsomes of untreated rats but neither isozyme wa...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Christou M,Wilson NM,Jefcoate CR

    更新日期:1987-11-01 00:00:00

  • Dietary flavonoid apigenin is a potential inducer of intracellular oxidative stress: the role in the interruptive apoptotic signal.

    abstract::Apigenin is a representative dietary flavone (2-phenyl-4H-1-benzopyran-4-one) inhibiting cancer cell growth both in cell culture systems and in vivo. The prooxidant potential of apigenin was confirmed by the observations using flowcytometric and immunoblotting techniques that the intracellular accumulations of reactiv...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Miyoshi N,Naniwa K,Yamada T,Osawa T,Nakamura Y

    更新日期:2007-10-15 00:00:00

  • ATP, histidine or magnesium ions can protect DNA against sisomicin-induced damage, following stray Cu(II) binding.

    abstract::The oxidative DNA damage by the cupric complexes of sisomicin was investigated in the presence of varying amounts of histidine, ATP, Mg(II) ions or phosphates. We found that by very low concentrations, the amino acid is able to inhibit the cleavage totally. This occurs both by its competition with antibiotic for coppe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Szczepanik W,Swiatek M,Skała J,Jezowska-Bojczuk M

    更新日期:2004-11-01 00:00:00

  • The solution structure of mucous glycoproteins: proton NMR studies of native and modified ovine submaxillary mucin.

    abstract::The solution structure of native and systematically modified ovine submaxillary mucin (OSM) has been probed by proton NMR spectroscopic methods. Most of the resonances in the spectra have been tentatively assigned to the peptide and O-linked disaccharide, alpha-N-acetylneuraminic acid 2----6 alpha-N-acetylgalactosamin...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gerken TA

    更新日期:1986-06-01 00:00:00

  • The role of sulfenic acids in cellular redox signaling: Reconciling chemical kinetics and molecular detection strategies.

    abstract::The reversible oxidation of protein cysteine residues is well recognized as an important regulatory mechanism in redox-dependent cell signaling. Cysteine oxidation is diverse in nature and involves various post-translational modifications (sulfenic acids, disulfides, etc.) and the specific functional or structural imp...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Heppner DE,Janssen-Heininger YMW,van der Vliet A

    更新日期:2017-02-15 00:00:00

  • Identification of the major regulatory phosphorylation site in sucrose-phosphate synthase.

    abstract::Sucrose-phosphate synthase (SPS; EC is regulated in part by reversible protein phosphorylation. When dephospho-SPS is partially purified from illuminated spinach leaves and incubated with [gamma-32P]ATP the enzyme is phosphorylated by a copurifying protein kinase. In this report, 32P-phosphopeptides from try...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: McMichael RW Jr,Klein RR,Salvucci ME,Huber SC

    更新日期:1993-12-01 00:00:00

  • Difference in polymerization and steady-state dynamics of free and gelsolin-capped filaments formed by alpha- and beta-isoactins.

    abstract::The polymerization of scallop beta-like actin is significantly slower than that of skeletal muscle alpha-actin. To reveal which steps of polymerization contribute to this difference, we estimated the efficiency of nucleation of the two actins, the rates of filament elongation at spontaneous and gelsolin-nucleated poly...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Khaitlina S,Hinssen H

    更新日期:2008-09-15 00:00:00

  • Expression of UDP-glucuronosyltransferases (UGTs) 2B7 and 1A6 in the human brain and identification of 5-hydroxytryptamine as a substrate.

    abstract::The extrahepatic expression of UDP-glucuronosyltransferases (UGTs) is important in the detoxification of a number of endogenous and exogenous compounds, including 5-hydroxytryptamine and morphine. Studies were designed to investigate the extrahepatic expression of human UGTs using RT-PCR techniques and to determine th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: King CD,Rios GR,Assouline JA,Tephly TR

    更新日期:1999-05-01 00:00:00

  • Cross talk between MMP2-Spm-Cer-S1P and ERK1/2 in proliferation of pulmonary artery smooth muscle cells under angiotensin II stimulation.

    abstract::The aim of the present study is to establish the mechanism associated with the proliferation of PASMCs under ANG II stimulation. The results showed that treatment of PASMCs with ANG II induces an increase in cell proliferation and 100 nM was the optimum concentration for maximum increase in proliferation of the cells....

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chowdhury A,Sarkar J,Pramanik PK,Chakraborti T,Chakraborti S

    更新日期:2016-08-01 00:00:00

  • Sarcolipin, the "proteolipid" of skeletal muscle sarcoplasmic reticulum, is a unique, amphipathic, 31-residue peptide.

    abstract::The sarcoplasmic reticulum of rabbit skeletal muscle contains a small "proteolipid," i.e., a protein which is soluble in acidic CHCl3/CH3OH. We propose the name sarcolipin for this small protein, to signify its lipid-like solubility and association with the sarcoplasmic reticulum. We have determined the following amin...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wawrzynow A,Theibert JL,Murphy C,Jona I,Martonosi A,Collins JH

    更新日期:1992-11-01 00:00:00

  • Physical and chemical characterization of a horse serum carboxylesterase.

    abstract::The serine carboxylesterase from horse serum was characterized by amino acid composition, peptide mapping, molecular and subunit weights, and sequencing of the amino acids around the essential serine residue at the active site. A protocol was developed for using reversed-phase high-performance liquid chromatography as...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Torres JL,Rush RS,Main AR

    更新日期:1988-11-15 00:00:00

  • Reconstitution of the lipid-depleted pyruvate oxidase system of Escherichia coli: the palmitic acid effect.

    abstract::Previous work has shown that the coupling of the soluble Escherichia coli pyruvate oxidase to a lipid-depleted membrane terminal electron transport system requires the addition of ubiquinone and a neutral lipid fraction (C. Cunningham and L. P. Hager (1975) J. Biol. Chem. 250, 7139-7146). The active factor present in ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kiuchi K,Hager LP

    更新日期:1984-09-01 00:00:00

  • Site-specific modification of hemoglobin by methyl acetyl phosphate.

    abstract::Methyl acetyl phosphate, which was originally synthesized as a site-specific reagent for hydroxybutyrate dehydrogenase [R. Kluger and W.-C. Tsui (1980) J. Org. Chem. 45, 2723], also has an affinity for the binding site for 2,3-diphosphoglycerate in hemoglobin. Three residues in or near this cleft between the beta-chai...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ueno H,Pospischil MA,Manning JM,Kluger R

    更新日期:1986-02-01 00:00:00

  • The plasma membrane Ca2+ ATPase of animal cells: structure, function and regulation.

    abstract::Most important processes in cell life are regulated by calcium (Ca2+). A number of mechanisms have thus been developed to maintain the concentration of free Ca2+ inside cells at the level (100-200nM) necessary for the optimal operation of the targets of its regulatory function. The systems that move Ca2+ back and fort...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Di Leva F,Domi T,Fedrizzi L,Lim D,Carafoli E

    更新日期:2008-08-01 00:00:00

  • The pH dependence of the kinetic parameters of ketol acid reductoisomerase indicates a proton shuttle mechanism for alkyl migration.

    abstract::The enzyme ketol acid reductoisomerase catalyzes the second common reaction in the biosynthesis of the branched chain amino acids. The reaction is complex as an alkyl migration and a ketone reduction apparently occur as separate steps during the conversion of acetolactate to 2,3-dihydroxy-3-methylbutyrate. This paper ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mrachko GT,Chunduru SK,Calvo KC

    更新日期:1992-05-01 00:00:00

  • Inhibition of ATPase activity of the recA protein by ATP ribose-modified analogs.

    abstract::The single-stranded, DNA-dependent ATPase activity of purified recA protein was found to be inhibited competitively by ribose-modified analogs of ATP, 3'-O-anthraniloyl-ATP (Ant-ATP), and 3'-O-(N-methylanthraniloyl)-ATP (Mant-ATP). The Ki values for Ant-ATP and Mant-ATP were around 7 and 3 microM at pH 7.5, respective...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Karasaki Y,Higashi K

    更新日期:1984-09-01 00:00:00

  • The fertilization layer mediated block to polyspermy in Xenopus laevis: isolation of the cortical granule lectin ligand.

    abstract::The fertilization layer of Xenopus laevis eggs is formed by the cortical granule lectin binding to its ligand. The binding requires Ca2+, is specific for galactose, and functionally establishes a block to polyspermy at fertilization. We have designed a new enzyme-linked lectin assay for the cortical granule lectin (CG...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Quill TA,Hedrick JL

    更新日期:1996-09-15 00:00:00

  • Detection of phenobarbital-induced cytochrome P-450 in rat hepatic microsomes using an enzyme-linked immunosorbent assay.

    abstract::The major phenobarbital-inducible form of cytochrome P-450 (cytochrome P-450 PB) was purified to homogeneity from rat liver microsomes and rabbit antibodies prepared against the purified enzyme. Using these antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed for the detection of cytochrome P-450 PB ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Seidel SL,Shawver LK,Shires TK

    更新日期:1984-03-01 00:00:00

  • Selective induction of heme oxygenase-1 isozyme in rat testis by human chorionic gonadotropin.

    abstract::A radioimmunoassay was developed to assess the response of testicular HO-1 to agents known to increase the microsomal heme oxygenase activity. Treatment of rats with human chorionic gonadotropin (hCG) increased the microsomal heme oxygenase activity in rat testis. The following data suggest that the increase was speci...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kutty RK,Maines MD

    更新日期:1989-01-01 00:00:00

  • The alpha 1-helix in the extra-membranous domain contributes to the stability of the iron-sulfur protein of the cytochrome bc1 complex.

    abstract::Previously, we reported that several charged amino acids located in the alpha1-beta4 loop of the iron-sulfur protein are required to maintain the stability of the protein and its assembly into the cytochrome bc1 complex. The current study extends this analysis to several amino acids localized in the single alpha-helix...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Obungu VH,Beattie DS

    更新日期:1998-11-01 00:00:00

  • EPR studies of spin-labeled bovine plasma amine oxidase: the nature of the substrate-binding site.

    abstract::The carbonyl cofactor of bovine plasma amine oxidase (EC, recently shown to be 6-hydroxydopa (also known as topa), has been spin labeled to the extent of one label per enzyme dimer molecule, using 4-amino-2,2,6,6-tetramethylpiperidine-N-oxyl (4-amino-TEMPO) and 4-hydrazino-TEMPO followed by reduction with bor...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Greenaway FT,O'Gara CY,Marchena JM,Poku JW,Urtiaga JG,Zou Y

    更新日期:1991-03-01 00:00:00

  • Molecular simulation of peptides coming of age: Accurate prediction of folding, dynamics and structures.

    abstract::The application of molecular dynamics simulations to study the folding and dynamics of peptides has attracted a lot of interest in the last couple of decades. Following the successful prediction of the folding of several proteins using molecular simulation, foldable peptides emerged as a favourable system mainly due t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Georgoulia PS,Glykos NM

    更新日期:2019-03-30 00:00:00

  • Fe(2+) induces a transient Ca(2+) release from rat liver mitochondria.

    abstract::Isolated mitochondria loaded with Ca(2+) and then exposed to Fe(2+) show a transient release of Ca(2+). The magnitude of this response depends on the Ca(2+) loading and the kinetics of the response depends on the concentration of added Fe(2+). We investigated the Fe(2+)-induced Ca(2+) release mechanism by measuring mi...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gogvadze V,Walter PB,Ames BN

    更新日期:2002-02-15 00:00:00

  • Binding and crossing: Methods for the characterization of membrane-active peptides interactions with membranes at the molecular level.

    abstract::Antimicrobial and cell-penetrating peptides have been the object of extensive studies for more than 60 years. Initially these two families were studied separately, and more recently parallels have been drawn. These studies have given rise to numerous methodological developments both in terms of observation techniques ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Emmanuelle S,Astrid W,Sandrine S,Sophie C,Nicolas R

    更新日期:2021-01-07 00:00:00