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L-Glutamate in the extracellular space regulates endogenous D-aspartate homeostasis in rat pheochromocytoma MPT1 cells.

Abstract：

:In previous studies [FEBS Lett. 434 (1998) 231, Arch. Biochem. Biophys. 404 (2002) 92], we demonstrated for the first time that D-aspartate (D-Asp) is synthesized in cultured mammalian cell lines, such as pheochromocytoma 12 (PC12) and its subclone, MPT1. Our current focus is analysis of the dynamics of D-Asp homeostasis in these cells. In this communication, we show that L-glutamate (Glu) and L-Glu transporter substrates in the extracellular space regulate the homeostasis of endogenous D-Asp in MPT1 cells. D-Asp is apparently in dynamic homeostasis, whereby endogenous D-Asp is constantly released into the extracellular space by an undefined mechanism, and continuously and intensively taken up into cells by an L-Glu transporter. Under these conditions, L-Glu and its transporter substrates in the medium may competitively inhibit the uptake of D-Asp via the transporter, resulting in accumulation of the amino acid in the extracellular space. We additionally demonstrate that DL-TBOA, a well-established L-Glu transporter inhibitor, is taken up by the transporter during long time intervals, but not on a short time-scale.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Adachi M,Koyama H,Long Z,Sekine M,Furuchi T,Imai K,Nimura N,Shimamoto K,Nakajima T,Homma H

doi

10.1016/j.abb.2004.01.016

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

89-96

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003986104000529

journal_volume

424

pub_type

杂志文章

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