1H nuclear magnetic resonance studies of the conformations of vitamin D compounds in various solvents.

abstract：:Bcl-2 family proteins regulate apoptosis through their homo- and heterodimerization. By protein sequence analysis and structural comparison, we have identified a conserved hydrophobic core at the BH1 and BH2 domains of Bcl-2 family proteins. The hydrophobic core is stabilized by hydrophobic interactions among the resi...

journal_title：Archives of biochemistry and biophysics

authors： Feng Y,Zhang L,Hu T,Shen X,Ding J,Chen K,Jiang H,Liu D

更新日期：2009-04-01 00:00:00

abstract：:The presence of multiple proteases in the culture filtrate of Streptomyces moderatus was detected. After preliminary purification by ammonium sulfate precipitation and decolorization using DEAE-cellulose, the fractionation of various proteases was carried out using CM-trisacryl cation-exchange chromatography. By this ...

journal_title：Archives of biochemistry and biophysics

authors： Chandrasekaran S,Dhar SC

更新日期：1987-09-01 00:00:00

abstract：:A chromatographic adsorbent (TED-Sepharose) capable of chelating various di- and trivalent metal ions was prepared by introducing tris(carboxymethyl)ethylenediamine into agarose. This gel was charged with Al3+, Ga3+, In3+, and Tl3+ to form immobilized-metal affinity adsorbents, and their adsorption behavior toward ser...

journal_title：Archives of biochemistry and biophysics

authors： Porath J,Olin B,Granstrand B

更新日期：1983-09-01 00:00:00

abstract：:A search of the chicken expressed sequence tag (EST) database identified 2 cDNA clones that appeared to represent members of the SULT1B and SULT1C enzyme families. These cDNAs were fully sequenced and found to contain full-length inserts. Phylogenetic analysis of the derived amino acid sequences clearly placed them as...

journal_title：Archives of biochemistry and biophysics

authors： Wilson LA,Reyns GE,Darras VM,Coughtrie MW

更新日期：2004-08-01 00:00:00

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:We have examined the effects of calcium and magnesium on both the structural characteristics and the self-association reaction of hyalin, a major protein component of the sea urchin extraembryonic matrix, the hyaline layer. In the absence of calcium, the circular dichroic spectrum revealed a protein possessing a high ...

journal_title：Archives of biochemistry and biophysics

authors： Rimsay R,Robinson JJ

更新日期：2003-06-15 00:00:00

abstract：:We propose that adverse effects of the antibiotic metronidazole may be due, wholly or in part, to its conversion to a thiamine analog and consequent vitamin B1 antagonism. Consistent with this hypothesis, the drug is accepted as a substrate for the thiaminase (EC 2.5.1.2) elaborated as an exoenzyme by the human gut fl...

journal_title：Archives of biochemistry and biophysics

authors： Alston TA,Abeles RH

更新日期：1987-09-01 00:00:00

abstract：:Quercetin is a flavonoid that has roles in both cytoprotection and cytotoxicity. The relation of queretin's cytoprotective and cytotoxic effects are unknown. Quercetin has been shown to induce expression of hypoxia-inducible factor, a protein that is known to regulate transcription of the erythropoietin (EPO) gene, an...

journal_title：Archives of biochemistry and biophysics

authors： Nishimura K,Matsumoto R,Yonezawa Y,Nakagawa H

更新日期：2017-12-15 00:00:00

abstract：:While prior studies focusing on male mice suggest a role for sterol carrier protein-2/sterol carrier protein-x (SCP-2/SCP-x; DKO) on hepatic phytol metabolism, its role in females is unresolved. This issue was addressed using female and male wild-type (WT) and DKO mice fed a phytoestrogen-free diet without or with 0.5...

journal_title：Archives of biochemistry and biophysics

authors： McIntosh AL,Storey SM,Huang H,Kier AB,Schroeder F

更新日期：2017-12-01 00:00:00

abstract：:A role of cysteine residues in annexin II tetramer (AIIt)'s function was investigated using the sulfhydryl reagent N-ethylmaleimide (NEM). Incubation of AIIt with NEM resulted in a dose-dependent inhibition of AIIt-mediated liposome aggregation and loss of sulfhydryl groups of AIIt. The concentration effecting 50% inh...

journal_title：Archives of biochemistry and biophysics

authors： Singh TK,Liu L

更新日期：2000-09-15 00:00:00

abstract：:Nonalcoholic fatty liver disease (NAFLD) is emerging as the most common liver disease in industrialized countries. Because hepatic steatosis is an early pathogenesis of NAFLD, the discovery of food components that could ameliorate hepatic steatosis is of interest. Susabinori (Pyropia yezoensis) is recognized as one of...

journal_title：Archives of biochemistry and biophysics

authors： Yanagita T,Tsuge K,Koga M,Inoue N,Nagao K

更新日期：2020-09-30 00:00:00

abstract：:Inorganic pyrophosphatase containing a pair of regulatory CBS domains (CBS-PPase1) is allosterically inhibited by AMP and ADP and activated by ATP and diadenosine polyphosphates. Mononucleotide binding to CBS domains and substrate binding to catalytic domains are characterized by positive co-operativity. Bioinformatic...

journal_title：Archives of biochemistry and biophysics

authors： Anashkin VA,Orlov VN,Lahti R,Baykov AA

更新日期：2019-02-15 00:00:00

abstract：:The three-dimensional conformation of a protein provides a wealth of biochemical information and with the advent of cloning techniques that allow the preparation of proteins almost at will, a renewed interest has arisen in the crystallographic determination of protein structures. As in any research technique, however,...

journal_title：Archives of biochemistry and biophysics

authors： Holden HM,Rayment I

更新日期：1991-11-15 00:00:00

abstract：:Malignancies are characterized by several drastic metabolic changes, one of which is a progressive rise in the levels of serum copper. This rise in serum copper is documented across all malignancies and across malignancies in several species. This study aims to explore in vitro the effect of increased copper levels on...

journal_title：Archives of biochemistry and biophysics

authors： Rizvi A,Furkan M,Naseem I

更新日期：2017-12-15 00:00:00

abstract：:An inorganic pyrophosphatase (PPase) was purified from thylakoid membrane of spinach leaves to electrophoretic purity by methods including detergent solubilization, ammonium sulfate fractionation, and successive chromatographic techniques. Current protocol yielded about 10% recovery of total activity with a 30-fold pu...

journal_title：Archives of biochemistry and biophysics

authors： Jiang SS,Fan LL,Yang SJ,Kuo SY,Pan RL

更新日期：1997-10-01 00:00:00

abstract：:The immunotoxic environmental pollutant tri-n-butyltin (TBT) kills thymocytes by apoptosis through a mechanism that requires an increase in intracellular Ca2+ concentration. The addition of TBT (EC50 = 2 microM) to fura-2-loaded rat thymocytes resulted in a rapid and sustained increase in the cytosolic free Ca2+ conce...

journal_title：Archives of biochemistry and biophysics

authors： Chow SC,Kass GE,McCabe MJ Jr,Orrenius S

更新日期：1992-10-01 00:00:00

abstract：:Procedures for the purification of bovine muscle carbonic anhydrase (isoenzyme III) are described. The purified enzyme has a molecular weight near 29,000 and contains one Zn2+ ion per molecule. The sedimentation coefficient, s(0)20,w, is 2.8 X 10(-13) s, the isoelectric pH is 8.5, and A280(0.1%) = 2.07 cm-1. The CO2 h...

journal_title：Archives of biochemistry and biophysics

authors： Engberg P,Millqvist E,Pohl G,Lindskog S

更新日期：1985-09-01 00:00:00

abstract：:Histidinol dehydrogenase (EC 1.1.1.23) activity was determined in several plant species and in cultured plant cell lines. The enzyme was purified from cabbage (Brassica oleracea) to apparent homogeneity. To render complete purification, a new, specific histidinol-Sepharose 4B affinity chromatography was developed. The...

journal_title：Archives of biochemistry and biophysics

authors： Nagai A,Scheidegger A

更新日期：1991-01-01 00:00:00

abstract：:The glucose transporter of the bacterial phosphotransferase system mediates sugar transport across the cytoplasmic membrane concomitant with sugar phosphorylation. It consists of a cytoplasmic subunit IIA(Glc) and the transmembrane subunit IICB(Glc). IICB(Glc) was purified to homogeneity by urea/alkali washing of memb...

journal_title：Archives of biochemistry and biophysics

authors： Zhuang J,Gutknecht R,Flükiger K,Hasler L,Erni B,Engel A

更新日期：1999-12-01 00:00:00

abstract：:Human sorcin, M(r) 22-kDa/pI 5.3, was identified in HOB1 lymphoma cells resistant to 1.0 microM vincristine and polyclonal antibody against it was produced. Using antibody to precipitate sorcin from 32P-labeled cell extract, the SDS-PAGE-resolved human sorcin was not phosphorylated in contrast to the report of A. M. v...

journal_title：Archives of biochemistry and biophysics

authors： Lee WP

更新日期：1996-01-15 00:00:00

abstract：:MicroRNA-342-3p (miR-342) has been shown to act as a tumor-suppressor in different cancer types. However, the role and therapeutic implications of miR-342 via modulation of Cofilin 1 (CFL1) has not been studied in any type of cancer. Given the importance of Cofilin signalling in breast, this study was undertaken to ex...

journal_title：Archives of biochemistry and biophysics

authors： Liu C,Xing H,Luo X,Wang Y

更新日期：2020-07-15 00:00:00

abstract：:Carbamoyl phosphate synthetase I (CPSase I) catalyzes the first reaction of the urea cycle in mammalian ureotelic species. The positive allosteric cofactor N-acetyl-L-glutamate (AGA) is required for CPSase I activity and is important for regulation of the urea cycle. A similar enzyme, CPSase III, catalyzes this reacti...

journal_title：Archives of biochemistry and biophysics

authors： Julsrud EA,Walsh PJ,Anderson PM

更新日期：1998-02-01 00:00:00

abstract：:Mammalian multidrug and toxic compound extrusion 1 (MATE1) are polyspecific H+-coupled exporters of organic cations (OCs) and responsible for excretion of metabolic waste products and xenobiotics. Here, we report a novel variant of mouse MATE1, mMATE1b, that has a long carboxyl terminal hydrophobic tail homologous to ...

journal_title：Archives of biochemistry and biophysics

authors： Kobara A,Hiasa M,Matsumoto T,Otsuka M,Omote H,Moriyama Y

更新日期：2008-01-15 00:00:00

abstract：:Calorie restriction (CR) has been shown to improve peripheral insulin resistance and type 2 diabetes in animal models. However, the exact mechanism of CR on GLUT4 expression and translocation in insulin-sensitive tissues has not been well elucidated. In the present study, we examine the effect of CR on the expression ...

journal_title：Archives of biochemistry and biophysics

authors： Park SY,Choi GH,Choi HI,Ryu J,Jung CY,Lee W

更新日期：2005-04-15 00:00:00

abstract：:The effects of nalidixic acid and hydroxyurea on nuclear and chloroplast DNA formation in cultured cells of Nicotiana tabacum were investigated. At low concentrations (5 and 20 micrograms/ml) nalidixic acid, an inhibitor of DNA gyrase, exhibited a greater inhibitory effect on plastid DNA synthesis than on nuclear DNA ...

journal_title：Archives of biochemistry and biophysics

authors： Heinhorst S,Cannon G,Weissbach A

更新日期：1985-06-01 00:00:00

abstract：:Guanidine hydrochloride-induced denaturation and thermal denaturation of three kinds of tryptophan synthase alpha subunit have been compared by circular dichroism measurements. The three alpha subunits are from Escherichia coli, Salmonella typhimurium, and an interspecies hybrid in which the C-terminal domain comes fr...

journal_title：Archives of biochemistry and biophysics

authors： Yutani K,Sato T,Ogasahara K,Miles EW

更新日期：1984-03-01 00:00:00

abstract：:Dark incubation of envelope vesicles derived from a strain of Halobacterium halobium that lacks bacteriorhodopsin but contains halorhodopsin and a third rhodopsin-like pigment caused a decrease in the flash yield [the amplitude of a transient absorbance change of flash reactive component(s) by flash] of halorhodopsin ...

journal_title：Archives of biochemistry and biophysics

authors： Kamo N,Hazemoto N,Kobatake Y,Mukohata Y

更新日期：1985-04-01 00:00:00

abstract：:Proliferation and migratory behavior of L929 murine fibroblasts were shown to be modified in the presence of a cytosolic extract of Phormidium sp. (Cyanobacteria). The addition of Phormidium extract to the growth medium (Dulbecco's modified Eagle's medium) supplemented with 0.5% newborn calf serum increased cell proli...

journal_title：Archives of biochemistry and biophysics

authors： Hodara ML,Bré MH,Bouzinba H,Pouphile M,Lefort-Tran M

更新日期：1991-04-01 00:00:00

abstract：:Application of nanosecond pulsed electric fields (nsPEFs) has attracted attention as a unique tool in life sciences, especially for cancer therapy, but the molecular mechanism of its action on living organisms is yet to be fully elucidated. Here, we report a transient activation of signaling pathways involving mitogen...

journal_title：Archives of biochemistry and biophysics

authors： Morotomi-Yano K,Akiyama H,Yano K

更新日期：2011-11-01 00:00:00

abstract：:To elucidate the physiological significance of ketone bodies on insulin and glucagon secretion, the direct effects of beta-hydroxybutyrate (BOHB) and acetoacetate (AcAc) infusion on insulin and glucagon release from perfused rat pancreas were investigated. The BOHB or AcAc was administered at concentrations of 10, 1, ...

journal_title：Archives of biochemistry and biophysics

authors： Ikeda T,Yoshida T,Ito Y,Murakami I,Mokuda O,Tominaga M,Mashiba H

更新日期：1987-08-15 00:00:00