Abstract：
:Quercetin is a flavonoid that has roles in both cytoprotection and cytotoxicity. The relation of queretin's cytoprotective and cytotoxic effects are unknown. Quercetin has been shown to induce expression of hypoxia-inducible factor, a protein that is known to regulate transcription of the erythropoietin (EPO) gene, and EPO is known to have a cytoprotective effect. This study used HepG2 cells to assess whether the cell-protective and/or cytotoxic roles of quercetin are mediated by promotion of EPO production. Increases in the levels of HIF-1α protein and EPO mRNA were quercetin concentration-dependent, with significant increases observed from 10 μM quercetin. Silencing of EPO expression by si-EPO RNA attenuated quercetin-induced cytoprotection against hydrogen peroxide toxicity. Cytotoxicity, evidenced by the induction of apoptosis, was significantly increased by exposure to 50 μM quercetin. Specifically, the levels of cleaved caspase-3 and Bax and the rate of cell death increased, and the level of Bcl-2 decreased, in cells treated with 50 μM quercetin. In contrast, exposure to 10 μM quercetin attenuated cisplatin-induced apoptosis. However, quercetin's ability to protect cells from cisplatin-induced apoptosis was eliminated when EPO expression was silenced using si-EPO RNA. Together, these results suggested that quercetin's cytoprotective effects in HepG2 cells are mediated via EPO production.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Nishimura K,Matsumoto R,Yonezawa Y,Nakagawa Hdoi
10.1016/j.abb.2017.10.013subject
Has Abstractpub_date
2017-12-15 00:00:00pages
11-16eissn
0003-9861issn
1096-0384pii
S0003-9861(17)30611-2journal_volume
636pub_type
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2018.04.004
更新日期：2018-06-01 00:00:00
