Abstract：
:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kinetics of in vitro mutagenesis of a number of these amino acids lining the PAH active site. Mutation of F299, Y324, F331, and Y343 caused a significant decrease in enzyme activity but no change in the Km for substrate or cofactor. We conclude that these aromatic residues are essential for activity but are not significantly involved in binding of the substrate or cofactor. In contrast the PAH mutant, S349T, showed an 18-fold increase in Km for phenylalanine, showing the first functional evidence that this residue was binding at or near the phenylalanine binding site. This confirms the recently published model for the binding of phenylalanine to the PAH active site that postulated S349 interacts with the amino group on the main chain of the phenylalanine molecule. This result differs with that found for the equivalent mutation (S395T), in the closely related tyrosine hydroxylase, which had no effect on substrate Km, showing that while the architecture of the two active sites are very similar the amino acids that bind to the respective substrates are different.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Jennings IG,Cotton RG,Kobe Bdoi
10.1006/abbi.2000.2111subject
Has Abstractpub_date
2000-12-15 00:00:00pages
238-44issue
2eissn
0003-9861issn
1096-0384pii
S0003-9861(00)92111-8journal_volume
384pub_type
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journal_title：Archives of biochemistry and biophysics
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journal_title：Archives of biochemistry and biophysics
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