Purification and electron microscopic characterization of the membrane subunit (IICB(Glc)) of the Escherichia coli glucose transporter.

abstract：:Nitric oxide formation by GH3 pituitary cells is stimulated by depolarizing concentrations of K+ and by the L-channel Ca2+ agonist Bay kappa 8644 in an additive manner that depends on extracellular Ca2+. Ca(2+)-dependent NO formation at 100 microM arginine was inhibited 50% over a 30-min period by 5 microM NG-amino-L-...

journal_title：Archives of biochemistry and biophysics

authors： Cooper GR,Barr A,Wolff DJ

更新日期：1998-09-15 00:00:00

abstract：:Experimental autoimmune encephalomyelitis (EAE) is a commonly used mouse model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelination leading to brain and spinal cord malfunctions. We postulate that not only biological but also biomechanical properties ...

journal_title：Archives of biochemistry and biophysics

authors： Pyka-Fościak G,Zemła J,Lis GJ,Litwin JA,Lekka M

更新日期：2020-02-15 00:00:00

abstract：BACKGROUND:Atherosclerosis is a chronic inflammatory disease and represents the leading cause of morbidity and mortality throughout the world. Accumulating evidences have showed that Dihydrocapsaicin (DHC) has been found to exert multiple pharmacological and physiological effects. Nevertheless, the effects and possible...

journal_title：Archives of biochemistry and biophysics

authors： Zhao JJ,Hu YW,Huang C,Ma X,Kang CM,Zhang Y,Guo FX,Lu JB,Xiu JC,Qiu YR,Sha YH,Gao JJ,Wang YC,Li P,Xu BM,Zheng L,Wang Q

更新日期：2016-08-15 00:00:00

abstract：:The modulation of the plasma membrane calcium pump (PMCA) gene expression is not well understood. We now present evidence that PMCA mRNA is induced by various hormones or agonists via multiple second messenger pathways that include protein kinase C-, cAMP-, and Ca(2+)-dependent mechanisms. Among several types of cultu...

journal_title：Archives of biochemistry and biophysics

authors： Kuo TH,Liu BF,Diglio C,Tsang W

更新日期：1993-09-01 00:00:00

abstract：:Advances in management have resulted in a dramatic decline in mortality for individuals infected with human immunodeficiency virus (HIV). This decrease in mortality, initially the result of improved prophylaxis and treatment of opportunistic infections but later mediated by the use of highly-active antiretroviral ther...

journal_title：Archives of biochemistry and biophysics

authors： Stone B,Dockrell D,Bowman C,McCloskey E

更新日期：2010-11-01 00:00:00

abstract：:Ferredoxin reductase (Fd-reductase) supplies reducing equivalents obtained from NADPH to mitochondrial cytochrome P450 enzymes via the small iron-sulfur protein ferredoxin. Two cDNAs (differing by the presence or absence of an 18-bp insert in the coding region) for the human Fd-reductase were subcloned into a newly co...

journal_title：Archives of biochemistry and biophysics

authors： Brandt ME,Vickery LE

更新日期：1992-05-01 00:00:00

abstract：:Nickel (Ni) is widely present in the occupational environment and causes various adverse effects on the human body. Apoptosis induced by Ni2+ may be a key mechanism underlying its toxic effect. In the present study, we investigated the effect of Ni-smelting fumes on cell viability, mitochondrial damage, and apoptosis-...

journal_title：Archives of biochemistry and biophysics

authors： Pan YL,Xin R,Wang SY,Wang Y,Zhang L,Yu CP,Wu YH

更新日期：2018-12-15 00:00:00

abstract：:The relationship between ligand-protein affinity and the extent of protein stabilization induced by such interactions has been investigated using the binding of polyanions to acidic fibroblast growth factor (aFGF) as a model system. It was found that the experimentally observed unfolding rate constant of aFGF consists...

journal_title：Archives of biochemistry and biophysics

authors： Mach H,Middaugh CR

更新日期：1994-02-15 00:00:00

abstract：:Proteolysis of glucose dehydrogenase from Bacillus megaterium with proteinase K apparently generated two fragments. The small fragment, designated as K-peptide, was sequenced and its covalent structure was determined as Ser-Ser-Glu-Ala-Ser-Tyr-Val-Thr-Gly-Ile-Thr-Leu-Phe-Ala-Asp-Gly-Gly-Met-Thr- Gln-Tyr-Pro-Ser-Phe-Gl...

journal_title：Archives of biochemistry and biophysics

authors： Jany KD,Nitsche E

更新日期：1984-02-15 00:00:00

abstract：:CYP27A1, an enzyme with several important roles in cholesterol homeostasis and vitamin D₃ metabolism, has been ascribed anti-atherogenic properties. This study addresses an important problem regarding how this enzyme, involved in cholesterol metabolism in the liver and peripheral tissues, is regulated. Our results ide...

journal_title：Archives of biochemistry and biophysics

authors： Norlin M,Pettersson H,Tang W,Wikvall K

更新日期：2011-02-15 00:00:00

abstract：:The three cysteine residues per subunit of pig muscle phosphoglucose isomerase show different reactivities toward various sulfhydryl reagents. The organomercurial, p-mercuribenzoate, can titrate two of the sulfhydryl groups under nondenaturing conditions. 2,2'-Dithiodipyridine, 5,5'-dithiobis(2-nitrobenzoic acid), iod...

journal_title：Archives of biochemistry and biophysics

authors： Scott-Ennis RJ,Noltmann EA

更新日期：1985-05-15 00:00:00

abstract：:G. metallireducens bacterium has highly versatile respiratory pathways that provide the microorganism an enormous potential for many biotechnological applications. However, little is known about the structural and functional properties of its electron transfer components. In this work, the periplasmic cytochrome PpcA ...

journal_title：Archives of biochemistry and biophysics

authors： Portela PC,Fernandes TM,Dantas JM,Ferreira MR,Salgueiro CA

更新日期：2018-04-15 00:00:00

abstract：:The ability for various ligands to modulate the binding of fructose 1,6-bisphosphate (Fru-1,6-P2) with purified rat liver pyruvate kinase was examined. Binding of Fru-1,6-P2 with pyruvate kinase exhibits positive cooperativity, with maximum binding of 4 mol Fru-1,6-P2 per enzyme tetramer. The Hill coefficient (nH), an...

journal_title：Archives of biochemistry and biophysics

authors： Blair JB,Walker RG

更新日期：1984-07-01 00:00:00

abstract：:With the concept of precision medicine, combining multiple molecular-targeting therapies has brought new approaches to current cancer treatments. Malfunction of the tumor suppressor protein, p53 is a universal hallmark in human cancers. Under normal conditions, p53 is degraded through an ubiquitin-proteosome pathway r...

journal_title：Archives of biochemistry and biophysics

authors： Chang SJ,Liao EC,Yeo HY,Kuo WH,Chen HY,Tsai YT,Wei YS,Chen YJ,Wang YS,Li JM,Shih CC,Chan CH,Lai ZY,Chou HC,Chuang YJ,Chan HL

更新日期：2018-06-01 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates the toxicity of dioxin and other xenobiotics. In the absence of exogenous ligand, AhR is cytosolic. We investigated how AhR is retained in the cytosol and how dioxin induces AhR to move to the nucleus. Disruption of nuclear ex...

journal_title：Archives of biochemistry and biophysics

authors： Richter CA,Tillitt DE,Hannink M

更新日期：2001-05-15 00:00:00

abstract：:Sensitive assays were developed for long chain and very long chain fatty acid oxidation in human skin fibroblast homogenates. Stearic and lignoceric acids were degraded by the fibroblasts by the beta-oxidation pathway. The cofactor requirements for stearic and lignoceric acid beta-oxidation were very similar but not i...

journal_title：Archives of biochemistry and biophysics

authors： Singh H,Poulos A

更新日期：1986-10-01 00:00:00

abstract：:A novel serum protein inhibiting specifically the enzymatic activity of the basic phospholipase A(2) (PLA(2)) from the venom of the Chinese mamushi snake (Agkistrodon blomhoffii siniticus) was purified from a nonvenomous Colubridae snake, Elaphe quadrivirgata. The purified inhibitor was a 150-kDa glycoprotein having a...

journal_title：Archives of biochemistry and biophysics

authors： Okumura K,Inoue S,Ikeda K,Hayashi K

更新日期：2002-12-01 00:00:00

abstract：:Glyceraldehyde (GA) has been used to study insulin secretion for decades and it is widely assumed that beta-cell metabolism of GA after its phosphorylation by triokinase is similar to metabolism of glucose; that is metabolism through distal glycolysis and oxidation in mitochondria. New data supported by existing infor...

journal_title：Archives of biochemistry and biophysics

authors： MacDonald MJ,Chaplen FW,Triplett CK,Gong Q,Drought H

更新日期：2006-03-15 00:00:00

abstract：:6-(Difluoromethyl)indole has been characterized and developed as a probe for the turnover of indole by the bifunctional enzyme, tryptophan synthase (alpha 2 beta 2). The neutral form of the indolyl species undergoes a slow and spontaneous hydrolysis to produce 6-formylindole with a rate constant (k1) of 0.0089 +/- 0.0...

journal_title：Archives of biochemistry and biophysics

authors： Woolridge EM,Rokita SE

更新日期：1991-05-01 00:00:00

abstract：:Catalytic activity of ISWI chromatin remodelers, which regulate nucleosome positioning on the DNA, depends on interactions of the putative acidic patch in ISWI helicase domain with the N-termini of nucleosomal H4--such, that removal of H4 termini abolishes ISWI remodeling. Acetylation of H4 termini is also known to di...

journal_title：Archives of biochemistry and biophysics

authors： Krajewski WA

更新日期：2016-02-01 00:00:00

abstract：:A quantitative assay has been developed to measure holocarboxylase synthetase activity in cellular extracts. This assay was based on measuring the incorporation of [3H]biotin of high specific activity (4.3 Ci/mmol) into purified rat liver apopyruvate carboxylase. With this assay, holocarboxylase synthetase in 3T3-L1 m...

journal_title：Archives of biochemistry and biophysics

authors： Chang HI,Cohen ND

更新日期：1983-08-01 00:00:00

abstract：:[3H]Diazonamide A ([3H]DZA), prepared from the natural product isolated from Diazona angulata, bound to tubulin in larger aberrant assembly products (>500 kDa by sizing HPLC) but not to the αβ-tubulin heterodimer. The binding reaction was rapid, but stoichiometry was low. Stoichiometry was enhanced up to 8-fold by pre...

journal_title：Archives of biochemistry and biophysics

authors： Bai R,Cruz-Monserrate Z,Fenical W,Pettit GR,Hamel E

更新日期：2020-02-15 00:00:00

abstract：:In trout and mammals, the major extrahepatic expression site for CYP3A forms is in the intestine. A cDNA encoding a new CYP3A subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (RT-PCR), followed by rapid amplification of cDNA ends (RACE)-PCR. In a set o...

journal_title：Archives of biochemistry and biophysics

authors： Lee SJ,Buhler DR

更新日期：2003-04-01 00:00:00

abstract：:The thermodynamic parameters of the alkaline transition for oxidized native yeast iso-1 cytochrome c and Rhodopseudomonas palustris cytochrome c(2) (cytc(2)) have been determined through direct electrochemistry experiments carried out at variable pH and temperature and compared to those for horse and beef heart cytoch...

journal_title：Archives of biochemistry and biophysics

authors： Battistuzzi G,Borsari M,Ranieri A,Sola M

更新日期：2002-08-15 00:00:00

abstract：:Changes in the levels of various molecular species of N-acylethanolamine in CdCl2-administered rat testis were examined. We found that the levels of various N-acylethanolamines including anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid receptor ligand, were dramatically increased in CdCl2-admin-ister...

journal_title：Archives of biochemistry and biophysics

authors： Kondo S,Sugiura T,Kodaka T,Kudo N,Waku K,Tokumura A

更新日期：1998-06-15 00:00:00

abstract：:Differential light absorption spectra of the bilirubin-albumin 1:1 complex, obtained on addition of 20 different sulfonamides, differ with respect to shape and amplitude. This finding seems to indicate that the sulfonamide molecule is bound in direct touch with the bilirubin. The light absorption spectrum of bilirubin...

journal_title：Archives of biochemistry and biophysics

authors： Brodersen R,Knudsen A,Pedersen AO

更新日期：1987-02-01 00:00:00

abstract：:Endothelial senescence is the main risk factor that contributes to vascular dysfunction and the progression of vascular disease. Carbon monoxide (CO) plays an important role in preventing vascular dysfunction and in maintaining vascular physiology or homeostasis. The application of exogenous CO has been shown to confe...

journal_title：Archives of biochemistry and biophysics

authors： Zheng M,Chen Y,Park J,Song HC,Chen Y,Park JW,Joe Y,Chung HT

更新日期：2019-11-30 00:00:00

abstract：:Ferroptosis is a type of iron-dependent, non-apoptotic cell death, which is typically induced by cysteine starvation or by the inhibition of glutathione peroxidase 4 (GPX4) activity with the accompanying elevation in lipid peroxidation product levels. Despite the central role of mitochondria in oxidative metabolism an...

journal_title：Archives of biochemistry and biophysics

authors： Homma T,Kobayashi S,Sato H,Fujii J

更新日期：2021-01-22 00:00:00

abstract：:The metabolite 5-aminolevulinic acid (ALA) is an early committed intermediate in the biosynthetic pathway of heme and chlorophyll formation. In plants, 5-aminolevulinic acid is synthesized via a two-step pathway in which glutamyl-tRNA(Glu) is reduced by glutamyl-tRNA(Glu) reductase (GluTR) to glutamate 1-semialdehyde,...

journal_title：Archives of biochemistry and biophysics

authors： Loida PJ,Thompson RL,Walker DM,CaJacob CA

更新日期：1999-12-15 00:00:00

abstract：:Uptake of L-[3H]glutamate by monolayers of fibroblasts cultured from human embryonic skin has been studied in the presence of several nonradioactive structural analogs of glutamate and aspartate. Results have suggested that the structural specificites of glutamate transporters in cultured human fibroblasts are similar...

journal_title：Archives of biochemistry and biophysics

authors： Cooper B,Chebib M,Shen J,King NJ,Darvey IG,Kuchel PW,Rothstein JD,Balcar VJ

更新日期：1998-05-15 00:00:00