Amino acid sequence of the K-peptide generated by limited proteolysis of glucose dehydrogenase from Bacillus megaterium by proteinase K1.

Abstract:

:Proteolysis of glucose dehydrogenase from Bacillus megaterium with proteinase K apparently generated two fragments. The small fragment, designated as K-peptide, was sequenced and its covalent structure was determined as Ser-Ser-Glu-Ala-Ser-Tyr-Val-Thr-Gly-Ile-Thr-Leu-Phe-Ala-Asp-Gly-Gly-Met-Thr- Gln-Tyr-Pro-Ser-Phe-Glu-Ala-Gly-Arg-Gly. The sequence analysis showed that the K-peptide consists of two identical fragments, one of which is lacking one serine residue at the amino-terminus.

journal_name

Arch Biochem Biophys

authors

Jany KD,Nitsche E

doi

10.1016/0003-9861(84)90162-0

subject

Has Abstract

pub_date

1984-02-15 00:00:00

pages

355-8

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(84)90162-0

journal_volume

229

pub_type

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