Abstract:
:Nanoceria were synthesized by discharging plasma at 800 V with a frequency of 30 kHz for 0-25 min using a pulsed unipolar power supply into solutions containing 1 or 2 mM of Ce(NO3)2. UV-Vis spectroscopy showed a characteristic absorbance maxima at 304-320 nm for the nanoceria with increase in the intensity of the peaks as the concentration of Ce(NO3)2 increased. The peaks exhibited transition red shift due to nanoceria formation. High resolution transmission electron microscopy revealed that spherical nanoparticles with an average size of 7.0 ± 0.2 nm were formed by discharging plasma for 15 min. The nanoceria showed excellent pH dependent antioxidant properties in hydroxyl and superoxide anion radical scavenging assays. Effect of the nanoceria on cell viability in vitro and inhibition of reactive oxygen species (ROS) by the nanoceria were examined using HeLa cell lines. As the results, no toxic effect was found up to 1600 μg mL-1 of nanoceria and they had an effective antioxidant property. Therefore, the nanoceria synthesized by one-step solution plasma process without employing hazardous chemicals have potential for utilization as antioxidant biomaterials and sustained release in the stream to scavenge ROS in the modern medicine.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Davoodbasha M,Park BR,Rhee WJ,Lee SY,Kim JWdoi
10.1016/j.abb.2018.02.003subject
Has Abstractpub_date
2018-05-01 00:00:00pages
42-49eissn
0003-9861issn
1096-0384pii
S0003-9861(17)30837-8journal_volume
645pub_type
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