Dihydrocapsaicin suppresses proinflammatory cytokines expression by enhancing nuclear factor IA in a NF-κB-dependent manner.

abstract：:5-Epi-aristolochene synthase is a sesquiterpene cyclase activity found in pathogen-challenged tobacco cells, but not in nonchallenged tissues, and appears to be encoded by a complex gene family. As a prerequisite to assessing the functional significance of these multiple genes, bacterial expression systems were examin...

journal_title：Archives of biochemistry and biophysics

authors： Back K,Yin S,Chappell J

更新日期：1994-12-01 00:00:00

abstract：:A novel bioluminescence-based solid-phase assay is described for the enzyme GDPFuc:Gal(beta)1-3GlcNAc (Fuc to GlcNAc) alpha1,4-fucosyltransferase (alpha1,4FT), which generates the Lewis a blood group antigen (Le(a)) (Gal(beta)1-3[Fuc(alpha)1-4]GlcNAc-R). Lacto-N-tetraose (LNT,Ga ta)1-3GlcNAc(beta)1-3Gal(beta)1-4Glc) w...

journal_title：Archives of biochemistry and biophysics

authors： DeBose-Boyd RA,Nyame AK,Smith DF,Cummings RD

更新日期：1996-11-01 00:00:00

abstract：:Atroxase, isolated from the venom of Crotalus atrox (western diamondback rattlesnake), is a nonhemorrhagic protease which has fibrinolytic activity in vivo. The primary structure of atroxase was deduced from the cDNA encoding the atroxase protein. The venom glands of Crotalus atrox were used to prepare a cDNA library....

journal_title：Archives of biochemistry and biophysics

authors： Baker BJ,Wongvibulsin S,Nyborg J,Tu AT

更新日期：1995-03-10 00:00:00

abstract：:A full-length cDNA for human placental thromboxane synthase and a shortened cDNA lacking the sequence corresponding to the N-terminal 2-29 amino acids were expressed in Escherichia coli using a pCW expression vector. Both intact and truncated recombinant enzyme were found in the membrane fraction and were catalyticall...

journal_title：Archives of biochemistry and biophysics

authors： Xia Z,Tai HH

更新日期：1995-08-20 00:00:00

abstract：:Rabbit antibodies have been prepared against ERp61, ERp59, and ERp49, three protein components of rough endoplasmic reticulum (RER) purified from mineral oil-induced plasmacytoma 315 (MOPC-315) tissue. Analysis of subcellular fractions of MOPC-315 tissue by an immunoprecipitation procedure demonstrated that all three ...

journal_title：Archives of biochemistry and biophysics

authors： Lewis MJ,Mazzarella RA,Green M

更新日期：1986-03-01 00:00:00

abstract：:Normal thymocytes and thymoma cells have been exposed to the toxic action of tert-butyl hydroperoxide (t-BOOH) to study the mechanisms of oxidative lethal cell injury and the sensitivity of normal and tumor cells to an oxidative stress. From our data, we can suggest that: (a) In thymoma cells two mechanisms can operat...

journal_title：Archives of biochemistry and biophysics

authors： Bartoli GM,Piccioni E,Agostara G,Calviello G,Palozza P

更新日期：1994-07-01 00:00:00

abstract：:Vitamin D was discovered as an anti-rachitic agent, but even at present, there is no direct evidence to support the concept that vitamin D directly stimulates osteoblastic bone formation and mineralization. It appears to be paradoxical, but vitamin D functions in the process of osteoclastic bone resorption. In 1952, C...

journal_title：Archives of biochemistry and biophysics

authors： Suda T,Takahashi F,Takahashi N

更新日期：2012-07-01 00:00:00

abstract：:Thrombin induces platelet activation through a variety of intracellular mechanisms, including Ca(2+) mobilization. The protein of the exocytotic machinery SNAP-25, but not VAMPs, is required for store-operated Ca(2+) entry, the main mechanism for Ca(2+) influx in platelets. Hence, we have investigated the role of the ...

journal_title：Archives of biochemistry and biophysics

authors： Jardin I,Ben Amor N,Hernández-Cruz JM,Salido GM,Rosado JA

更新日期：2007-09-01 00:00:00

abstract：:The stoichiometry of complex formation between two lysosomal proteinases from rabbit liver, cathepsin M and fructose 1,6-bisphosphatase converting enzyme (CE), and their respective endogenous inhibitors was studied by the equilibrium gel penetration method. In each case the molecular weight of the complex was found to...

journal_title：Archives of biochemistry and biophysics

authors： Pontremoli S,Melloni E,Salamino F,Sparatore B,Michetti M,Horecker BL

更新日期：1984-02-01 00:00:00

abstract：:The hexapeptide Hyp-Gly-Pro-Lys-Gly-Glu was synthesized as a potential substrate for collagen lysyl hydroxylase. Kinetic data on the interaction of this peptide with purified chicken embryo lysyl hydroxylase showed that the hexapeptide is a moderately good substrate having Km, Vmax, and Kcat/Km values comparable to th...

journal_title：Archives of biochemistry and biophysics

authors： Ananthanarayanan VS,Saint-Jean A,Jiang P

更新日期：1992-10-01 00:00:00

abstract：:Oxidative stress is a mechanism with a central role in the pathogenesis of atherosclerosis, cancer, and other chronic diseases. It also plays a major role in the aging process. Ischemic heart disease is perhaps the human condition in which the role of oxidative stress has been investigated in more detail: reactive oxy...

journal_title：Archives of biochemistry and biophysics

authors： Ceconi C,Boraso A,Cargnoni A,Ferrari R

更新日期：2003-12-15 00:00:00

abstract：:Purification of equilibrative nucleoside transporters has been hampered by the functional instability of the detergent-solubilized proteins. A variety of nonionic detergents were compared with octylglucoside (the most commonly used detergent in this regard) for their abilities to solubilize functionally stable nucleos...

journal_title：Archives of biochemistry and biophysics

authors： Hammond JR,Zarenda M

更新日期：1996-08-15 00:00:00

abstract：:Protoporphyrin IX inhibits citrulline formation by all three nitric oxide synthase isoforms in a manner reversible by dilution. Zinc protoporphyrin IX, by contrast, produces a time- and concentration-dependent inactivation of all three nitric oxide synthase isoforms, not reversible by dilution. The inhibition of citru...

journal_title：Archives of biochemistry and biophysics

authors： Wolff DJ,Naddelman RA,Lubeskie A,Saks DA

更新日期：1996-09-01 00:00:00

abstract：:Purple Cu(A) centers are a class of binuclear, mixed-valence copper complexes found in cytochrome c oxidase and nitrous oxide reductase. An engineered Cu(A) protein was formed by replacing a portion of the amino acid sequence that contains three of the ligands to the native type I copper center of Pseudomonas aerugino...

journal_title：Archives of biochemistry and biophysics

authors： Sun D,Wang X,Davidson VL

更新日期：2002-08-01 00:00:00

abstract：:The P-III class of venom metalloproteinases has, in addition to the proteinase domain, a disintegrin-like domain and a cysteine-rich domain. Recent evidence has shown that the nonproteinase domains of the P-III class of hemorrhagic metalloproteinases function in the inhibition of platelet aggregation by blocking essen...

journal_title：Archives of biochemistry and biophysics

authors： Jia LG,Wang XM,Shannon JD,Bjarnason JB,Fox JW

更新日期：2000-01-01 00:00:00

abstract：:To establish a cell line expressing enhanced levels of beta-amyloid precursor protein (beta-APP), we constructed plasmid DNAs expressing beta-APP-751 mRNA and transfected them into COS-1 cells. Using a modified version of the reverse transcriptase polymerase chain reaction which is RNA sensitive to study the beta-APP ...

journal_title：Archives of biochemistry and biophysics

authors： Ramakrishna N,Smedman M,Gillam B

更新日期：1996-02-15 00:00:00

abstract：:We previously demonstrated that the expression of HSP20, a small heat shock protein, is inversely correlated with the progression of HCC. Inflammation is associated with HCC, and numerous cytokines, including TNF-α, act as key mediators in the progression of HCC. In the present study, we investigated whether HSP20 is ...

journal_title：Archives of biochemistry and biophysics

authors： Nagasawa T,Matsushima-Nishiwaki R,Yasuda E,Matsuura J,Toyoda H,Kaneoka Y,Kumada T,Kozawa O

更新日期：2015-01-01 00:00:00

abstract：:In this study, we have analyzed interflavin electron transfer reactions from FAD to FMN in both the full-length inducible nitric oxide synthase (iNOS) and its reductase domain. Comparison is made with the interflavin electron transfer in NADPH-cytochrome P450 reductase (CPR). For the analysis of interflavin electron t...

journal_title：Archives of biochemistry and biophysics

authors： Yamamoto K,Kimura S,Shiro Y,Iyanagi T

更新日期：2005-08-01 00:00:00

abstract：:Enzymes subjected to freeze-thawing are known to be protected by polymers that are preferentially excluded from the hydrated surface of proteins [reviewed in Carpenter et al. (1994) ACS Symp. Ser. 567, 134-147]. Preferentially excluded solutes are also known to stabilize quaternary structure, which enhances the thermo...

journal_title：Archives of biochemistry and biophysics

authors： Anchordoquy TJ,Carpenter JF

更新日期：1996-08-15 00:00:00

abstract：:The ability of nine phospholipids to alter the activity of low-Km cyclic AMP phosphodiesterase was examined in microsomal fractions of rat adipocytes. The enzyme was activated by phosphatidylserine (21% at 300 microM) and phosphatidylglycerol (36% at 300 microM). The activation was concentration dependent over the ran...

journal_title：Archives of biochemistry and biophysics

authors： Macaulay SL,Kiechle FL,Jarett L

更新日期：1983-08-01 00:00:00

abstract：:cDNA clones encoding NADP(+)-glyceraldehyde-3-phosphate dehydrogenase (NADP(+)-GAPDH) and sedoheptulose-1,7-bisphosphatase (SBPase) were isolated and characterized from halotolerant Chlamydomonas sp. W80 (C. W80) cells. The cDNA clone for NADP(+)-GAPDH encoded 369 amino acid residues, preceded by the chloroplast trans...

journal_title：Archives of biochemistry and biophysics

authors： Tamoi M,Kanaboshi H,Miyasaka H,Shigeoka S

更新日期：2001-06-15 00:00:00

abstract：:Using ammonium sulfate precipitation, gel filtration, and affinity chromatography, inosine monophosphate (IMP) oxidoreductase (EC 1.2.1.14) was isolated from the soluble proteins of the plant cell fraction of nitrogen-fixing nodules of cowpea (Vigna unguiculata L. Walp). The enzyme, purified more than 140-fold with a ...

journal_title：Archives of biochemistry and biophysics

authors： Atkins CA,Shelp BJ,Storer PJ

更新日期：1985-02-01 00:00:00

abstract：:Phenolic phytochemicals such as tannins, which are natural constituents of green tea, red wine, and other plant products, are considered to have cancer-preventive properties. An important endogenous mediator of tumorigenesis is the nuclear enzyme poly(ADP-ribose) polymerase 1 (PARP-1). PARP-1 synthesizes polymers of A...

journal_title：Archives of biochemistry and biophysics

authors： Keil C,Petermann E,Oei SL

更新日期：2004-05-01 00:00:00

abstract：:An alkaline proteinase, identical to mast cell chymase, has been described by a number of laboratories as being associated with myofibrils extracted from adult rat skeletal muscle tissue. A more recent study has indicated that chymase may be an intrinsic protein in the rat myocyte. The present study of rat myogenic ce...

journal_title：Archives of biochemistry and biophysics

authors： Hartz PA,Bird JW

更新日期：1988-06-01 00:00:00

abstract：:Increasing evidence suggests that long non-coding RNAs (lncRNAs) are implicated with chemoresistance of cancers. However, their functional role and molecular mechanisms in colorectal cancer (CRC) chemoresistance are still largely unclear. In this work, we aimed to investigate the functional role of lncRNA cancer susce...

journal_title：Archives of biochemistry and biophysics

authors： Gao R,Fang C,Xu J,Tan H,Li P,Ma L

更新日期：2019-03-15 00:00:00

abstract：:Two histone acetyltransferases, A and B, have been extracted and partially purified from yeast cells. The purification scheme included ammonium sulfate precipitation, and chromatography on DEAE-Sepharose and Sephadex G-200. The basic properties of both enzymes closely correspond to those of acetyltransferase A and B f...

journal_title：Archives of biochemistry and biophysics

authors： Lopez-Rodas G,Perez-Ortin JE,Tordera V,Salvador ML,Franco L

更新日期：1985-05-15 00:00:00

abstract：:The zaragozic acids are potent inhibitors of squalene synthase. In vivo studies in mice confirmed our earlier observations that inhibition of squalene synthase by zaragozic acid A was accompanied by an increase in the incorporation of label from [3H]mevalonate into farnesyl-diphosphate (FPP)-derived isoprenoic acids (...

journal_title：Archives of biochemistry and biophysics

authors： Vaidya S,Bostedor R,Kurtz MM,Bergstrom JD,Bansal VS

更新日期：1998-07-01 00:00:00

abstract：:Phosphatidylethanols (PEths) are negatively charged diacyl phospholipids that are ubiquitously present in humans under the condition of alcohol intoxication. These lipids, derived in vivo from other naturally occurring phospholipids such as phosphatidylcholines (PC) via transphosphatidylation reaction as catalyzed by ...

journal_title：Archives of biochemistry and biophysics

authors： Li S,Lin H,Wang G,Huang CH

更新日期：2001-01-01 00:00:00

abstract：:During the metabolism of 1,2-dimethylhydrazine (a colon carcinogen) in rats, methyl radicals (.CH3) are produced. To gain an insight into the nature of .CH3 interactions with biomolecules in vivo, we conducted in vitro studies using RNA as a model compound. RNA was incubated in a system where .CH3 was generated from t...

journal_title：Archives of biochemistry and biophysics

authors： Kang JO,Gallagher KS,Cohen G

更新日期：1993-10-01 00:00:00

abstract：:The localization, expression, and transport of two lysosomal integral membrane glycoproteins of human cells, hLAMP-1 and hLAMP-2, have been studied in mucolipidosis II (I-cell disease) fibroblasts. These cells are deficient in N-acetylglucosaminylphosphotransferase, one of the enzymes required for addition of the mann...

journal_title：Archives of biochemistry and biophysics

authors： Sandoval IV,Chen JW,Yuan L,August JT

更新日期：1989-05-15 00:00:00