Involvement of SNARE proteins in thrombin-induced platelet aggregation: evidence for the relevance of Ca2+ entry.

abstract：:Biotin sulfoxide reductase catalyzes the conversion of d-biotin d-sulfoxide (BSO) to d-biotin. Oligonucleotides directed against common sequences in Escherichia coli biotin sulfoxide reductase and in Rhodobacter sphaeroides f.sp. denitrificans dimethyl sulfoxide reductase have been utilized to amplify by PCR a 651-bp ...

journal_title：Archives of biochemistry and biophysics

authors： Pollock VV,Barber MJ

更新日期：1995-04-20 00:00:00

abstract：:Guanidine hydrochloride-induced denaturation and thermal denaturation of three kinds of tryptophan synthase alpha subunit have been compared by circular dichroism measurements. The three alpha subunits are from Escherichia coli, Salmonella typhimurium, and an interspecies hybrid in which the C-terminal domain comes fr...

journal_title：Archives of biochemistry and biophysics

authors： Yutani K,Sato T,Ogasahara K,Miles EW

更新日期：1984-03-01 00:00:00

abstract：:Cortactin is an F-actin binding protein that contributes to cytoskeleton remodelling. We identified five isoforms of mouse cortactin that differ in the number of tandem 37-amino acid repeats, named cortactin repeats. The transcription of minor isoforms with 4.5, 3.5 or 2.5 cortactin repeats was low in most adult tissu...

journal_title：Archives of biochemistry and biophysics

authors： Katsube T,Togashi S,Hashimoto N,Ogiu T,Tsuji H

更新日期：2004-07-01 00:00:00

abstract：:Nitric oxide formation by GH3 pituitary cells is stimulated by depolarizing concentrations of K+ and by the L-channel Ca2+ agonist Bay kappa 8644 in an additive manner that depends on extracellular Ca2+. Ca(2+)-dependent NO formation at 100 microM arginine was inhibited 50% over a 30-min period by 5 microM NG-amino-L-...

journal_title：Archives of biochemistry and biophysics

authors： Cooper GR,Barr A,Wolff DJ

更新日期：1998-09-15 00:00:00

abstract：:A technique is presented for the preparation of three major proteoglycans from 14-day embryonic chicken retinas following their culture overnight with [35S]sulfate and either [3H]glucosamine or [3H]serine. Homogenization of the tissue in saline permitted extraction of heterogeneous soluble proteoglycans separately fro...

journal_title：Archives of biochemistry and biophysics

authors： Morris JE

更新日期：1984-11-15 00:00:00

abstract：:The keto form of oxaloacetate (OAA), a product of phosphoenolpyruvate carboxylase (PEPC) activity, can undergo various nonenzymatic conversions which make conventional methods of assaying the enzyme difficult, because the products may either act as inhibitors or go undetected. In studies with PEPC isolated from leaves...

journal_title：Archives of biochemistry and biophysics

authors： Walker GH,Ku MS,Edwards GE

更新日期：1986-08-01 00:00:00

abstract：:Juvenile hormone binding protein (JHBP) acts as a shuttle, carrying one of the most crucial hormones for insect development to target tissues. We have found that although the JHBP molecule does not contain tryptophan residues, it exhibits a weak fluorescence maximum near 420nm upon excitation at 315nm. Gel filtration ...

journal_title：Archives of biochemistry and biophysics

authors： Bystranowska D,Szewczuk Z,Lisowski M,Sitkiewicz E,Dobryszycki P,Ożyhar A,Kochman M

更新日期：2012-01-01 00:00:00

abstract：:A number of naturally occurring small organic molecules, primarily involved in maintaining osmotic pressure in the cell, display chaperone-like activity, stabilizing the native conformation of proteins, and protecting them from various kinds of stress. Most of them are sugars, polyols, amino acids or methylamines. Sim...

journal_title：Archives of biochemistry and biophysics

authors： Zancan P,Sola-Penna M

更新日期：2005-12-01 00:00:00

abstract：:This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol-protein ratio (C/Pt). The membrane fluidity, as assessed by a ...

journal_title：Archives of biochemistry and biophysics

authors： Dumas D,Muller S,Gouin F,Baros F,Viriot ML,Stoltz JF

更新日期：1997-05-01 00:00:00

abstract：:The sites of expression in the small intestine and the function of CYP2J4, a recently identified rat cytochrome (P450) isoform found to be predominantly expressed in the small intestine, were characterized. Immunoblot analysis with a polyclonal antibody to heterologously expressed CYP2J4 revealed that expression of CY...

journal_title：Archives of biochemistry and biophysics

authors： Zhang QY,Raner G,Ding X,Dunbar D,Coon MJ,Kaminsky LS

更新日期：1998-05-15 00:00:00

abstract：:A new plant lectin from elderberry (Sambucus nigra L.) bark, which was shown by immunochemical techniques to bind specifically to terminal Neu5Ac(alpha 2-6)Gal/GalNAc residues of glycoconjugates, was immobilized onto Sepharose 4B (SNA-Sepharose) and its carbohydrate binding properties was determined using a series of ...

journal_title：Archives of biochemistry and biophysics

authors： Shibuya N,Goldstein IJ,Broekaert WF,Nsimba-Lubaki M,Peeters B,Peumans WJ

更新日期：1987-04-01 00:00:00

abstract：:The stability of the Glossoscolex paulistus hemoglobin (HbGp), in two iron oxidation states (and three forms), as monitored by optical absorption, fluorescence emission and circular dichroism (CD) spectroscopies, in the presence of the chaotropic agent urea, is studied. HbGp oligomeric dissociation, denaturation and i...

journal_title：Archives of biochemistry and biophysics

authors： Carvalho FA,Santiago PS,Tabak M

更新日期：2012-03-01 00:00:00

abstract：:Vanadate-dependent oxidation of NADH by xanthine oxidase does not require the presence of xanthine and therefore is not due to cooxidation. Addition of NADH or xanthine had no effect on the oxidation of the other substrate. Oxidation of NADH was high at acid pH and oxidation of xanthine was high at alkaline pH. The sp...

journal_title：Archives of biochemistry and biophysics

authors： Khandke L,Gullapalli S,Patole MS,Ramasarma T

更新日期：1986-02-01 00:00:00

abstract：:Liver mitochondria were incubated through extended State 3-State 4 transitions (B. Chance and G. N. Williams (1955) J. Biol. Chem. 217, 409-423) in the presence of high concentrations of adenine nucleotides and in presence and absence of a protonophore. In the terminal phase of these transitions (the region of respira...

journal_title：Archives of biochemistry and biophysics

authors： Davis EJ,Davis-van Thienen WI

更新日期：1989-12-01 00:00:00

abstract：:Proliferation and migratory behavior of L929 murine fibroblasts were shown to be modified in the presence of a cytosolic extract of Phormidium sp. (Cyanobacteria). The addition of Phormidium extract to the growth medium (Dulbecco's modified Eagle's medium) supplemented with 0.5% newborn calf serum increased cell proli...

journal_title：Archives of biochemistry and biophysics

authors： Hodara ML,Bré MH,Bouzinba H,Pouphile M,Lefort-Tran M

更新日期：1991-04-01 00:00:00

abstract：:Thyroid peroxidase (TPO) was purified from human thyroid tissue, obtained at surgery from patients with Graves' disease, by a procedure similar to one that we had previously used for the purification of porcine TPO. The membrane-bound enzyme was solubilized by treatment of the thyroid particulate fraction with trypsin...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris ML,Yokoyama N,Slaughter C

更新日期：1990-05-01 00:00:00

abstract：:Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, stimulates both the cellular uptake of radiolabeled choline and the activity of CTP:phosphocholine cytidylyltransferase resulting in increased incorporation of choline into phosphatidylcholine (PtdCho). Because of these multiple actions, it...

journal_title：Archives of biochemistry and biophysics

authors： Kiss Z

更新日期：1996-11-01 00:00:00

abstract：:Phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, Helix pomatia (gastropoda), and mussels, Mytilus edulis (bivalvia). Under denaturing conditions, both enzymes had a molecular mass of 82 kDa. In the presence of ATP-Mg2+, the enzymes were rapidly phosphorylated in vitro by th...

journal_title：Archives of biochemistry and biophysics

authors： Biethinger M,Hoffmann R,Hofer HW

更新日期：1991-06-01 00:00:00

abstract：:Procedures for the purification of bovine muscle carbonic anhydrase (isoenzyme III) are described. The purified enzyme has a molecular weight near 29,000 and contains one Zn2+ ion per molecule. The sedimentation coefficient, s(0)20,w, is 2.8 X 10(-13) s, the isoelectric pH is 8.5, and A280(0.1%) = 2.07 cm-1. The CO2 h...

journal_title：Archives of biochemistry and biophysics

authors： Engberg P,Millqvist E,Pohl G,Lindskog S

更新日期：1985-09-01 00:00:00

abstract：:Fumarase catalyzes the reversible, stereospecific hydration of fumarate to form L-malate. We have determined the viscosity dependence of V/K and V in both the forward and the reverse directions at pH 6.9 in the absence and presence of several viscosogenic reagents. V/K for fumarate hydration decreases with increasing ...

journal_title：Archives of biochemistry and biophysics

authors： Sweet WL,Blanchard JS

更新日期：1990-02-15 00:00:00

abstract：:Purified NAD-malic enzyme from Ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mM borate. Determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedio...

journal_title：Archives of biochemistry and biophysics

authors： Rao GS,Kong CT,Benjamin RC,Harris BG,Cook PF

更新日期：1987-05-15 00:00:00

abstract：:The decomposition of S-nitrosoglutathione (GSNO) in the presence of Cu2+ and glutathione (GSH) was studied by stopped-flow/rapid-scan spectroscopy. Reduction of Cu2+ by GSH and subsequent formation of a GS-*Cu+ complex occurred within 200 ms, with the amount of complex formed depending on the GSH-to-Cu2+ ratio. The ra...

journal_title：Archives of biochemistry and biophysics

authors： Gorren AC,Schrammel Astrid,Schmidt K,Mayer B

更新日期：1996-06-15 00:00:00

abstract：:The in vivo binding of platinum to metallothionein (MT) has been observed in rat tissues following injections of the cis and trans isomers of DDP (dichlorodiammineplatinum(II]. Platinum in either cis-DDP or trans-DDP does not directly induce MT; platinum-MT is produced by the replacement of previously bound zinc in th...

journal_title：Archives of biochemistry and biophysics

authors： Zelazowski AJ,Garvey JS,Hoeschele JD

更新日期：1984-02-15 00:00:00

abstract：:The vesicle-to-micelle structural transitions that occurred in the interaction of sodium dodecyl sulfate with phosphatidylcholine vesicles were studied at the equilibrium by means of dynamic light scattering (at different scattering angles) and freeze-fracture electron microscopy techniques. The incorporation of surfa...

journal_title：Archives of biochemistry and biophysics

authors： López O,Cócera M,Wehrli E,Parra JL,de la Maza A

更新日期：1999-07-15 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that functions as an intracellular mediator in the xenobiotic signaling pathway. Although a number of studies have examined AhR-mediated CYP1A1 induction in detail, recent studies of AhR-null mice have revealed that AhR plays important regu...

journal_title：Archives of biochemistry and biophysics

authors： Kawajiri K,Fujii-Kuriyama Y

更新日期：2007-08-15 00:00:00

abstract：:Fluidity of the red blood cell membrane decreases as pH changes from 8 to 7.5. In rat liver mitochondrial (RLM) membrane fluidity precipitously declines as pH drops from 7.35 toward 7.0. With dithionitrobenzoate (Nbs2), reaction rates of mitochondrial -SH groups from rat liver and heart (RHM) and in beef heart submito...

journal_title：Archives of biochemistry and biophysics

authors： Zimmer G,Freisleben HJ,Fuchs J

更新日期：1990-11-01 00:00:00

abstract：:Purified 125I-labeled lectins from Datura stramonium, Evonymus europaea, and Griffonia simplicifolia (I-B4 isolectin) were used to analyze changes in the expression of carbohydrates on the surface of resident (PC) and thioglycollate-stimulated murine (C57B/6J) peritoneal exudate cells (PEC). The lectins from D. stramo...

journal_title：Archives of biochemistry and biophysics

authors： Petryniak J,Huard TK,Nordblom GD,Goldstein IJ

更新日期：1986-01-01 00:00:00

abstract：:The binding of the ligands Mn2+, Ca2+, and methyl alpha-D-glucopyranoside to concanavalin A, purified as described (A.J. Sophianopoulos and J.A. Sophianopoulos (1981) Prep. Biochem. 11, 413-435), was studied by ultrafiltration in 0.2 M NaCl, pH 5.2 and pH 6.5 to 7, and at 23 to 25 degrees C. The association constant (...

journal_title：Archives of biochemistry and biophysics

authors： Sophianopoulos JA,Sophianopoulos AJ,MacMahon WC

更新日期：1983-06-01 00:00:00

abstract：:We have previously demonstrated that dietary fat, especially unsaturated fatty acids, induces cellular retinol-binding protein, type II (CRBPII) gene expression in rat jejunum. In the present study, we showed that feeding a high-fat diet caused parallel increases in jejunal CRBPII mRNA and CRBPII pre-mRNA levels. Nucl...

journal_title：Archives of biochemistry and biophysics

authors： Suruga K,Mochizuki K,Kitagawa M,Goda T,Horie N,Takeishi K,Takase S

更新日期：1999-02-01 00:00:00

abstract：:Cellular glutathione is released during apoptosis and may play a role in the regulation of the mitochondrial permeability transition pore. The question of whether only cytosolic glutathione is important in apoptosis, or whether mitochondrial glutathione also plays a role, was investigated using gamma-glutamyltranspept...

journal_title：Archives of biochemistry and biophysics

authors： Will Y,Kaetzel RS,Brown MK,Fraley TS,Reed DJ

更新日期：2002-01-15 00:00:00