Bacterial expression of functional membrane-bound thromboxane synthase having intact sequence and truncated N-terminal hydrophobic segment.

abstract：:Phosphatidylethanols (PEths) are negatively charged diacyl phospholipids that are ubiquitously present in humans under the condition of alcohol intoxication. These lipids, derived in vivo from other naturally occurring phospholipids such as phosphatidylcholines (PC) via transphosphatidylation reaction as catalyzed by ...

journal_title：Archives of biochemistry and biophysics

authors： Li S,Lin H,Wang G,Huang CH

更新日期：2001-01-01 00:00:00

abstract：:Thymidylate synthase (TS) is an essential enzyme that must be expressed in all proliferating cells. The mouse TS promoter lacks a TATA box and an initiator element and initiates transcription over a broad region in cultured fibroblasts. The goal of this study was to determine if expression of the TS gene in a variety ...

journal_title：Archives of biochemistry and biophysics

authors： Lee Y,Shen G,Johnson LF

更新日期：1999-12-15 00:00:00

abstract：:The skin is the largest organ of the body that produces a flexible and self-repairing barrier and protects the body from most common potentially harmful physical, environmental, and biological insults. Solar ultraviolet (UV) radiation is one of the major environmental insults to the skin and causes multi-tiered cellul...

journal_title：Archives of biochemistry and biophysics

authors： Afaq F

更新日期：2011-04-15 00:00:00

abstract：:The mechanism by which chemical inducers of the stress response inhibit protein synthesis was examined. All the chemicals tested principally inhibit the initiation phase of translation. Covalent modification of the initiation factor proteins does not constitute a common mechanism. Eukaryotic initiation factor (eIF)-2 ...

journal_title：Archives of biochemistry and biophysics

authors： Duncan RF,Hershey JW

更新日期：1987-08-01 00:00:00

abstract：:Cancer osaka thyroid (COT), a human MAP 3 K, is essential for lipopolysaccharide activation of the Erk MAPK cascade in macrophages. COT 30--467 is insoluble, whereas low levels of COT 30--397 can be expressed, but this protein is unstable. However, both COT 30--467 and COT 30--397 are expressed in a soluble and stable...

journal_title：Archives of biochemistry and biophysics

authors： Jia Y,Quinn CM,Bump NJ,Clark KM,Clabbers A,Hardman J,Gagnon A,Kamens J,Tomlinson MJ,Wishart N,Allen H

更新日期：2005-09-01 00:00:00

abstract：:Actinomycin D (act-D) anchors itself into DNA-base pairs by intercalation and thereby inhibits mRNA synthesis. It has been well established that act-D elicits apoptosis in various cell types involving endothelial cells. However, the regulatory mechanisms of actinomycin D-induced apoptotic cell death still remain uncle...

journal_title：Archives of biochemistry and biophysics

authors： Shim D,Kang HY,Jeon BW,Kang SS,Chang SI,Kim HY

更新日期：2004-05-15 00:00:00

abstract：:ATP-activated P2Y receptors play an important role in renal sodium excretion. The aim of this study was to evaluate the modulation of ATPase-driven sodium reabsorption in the proximal tubule by ATP or adenosine (Ado). LLC-PK1 cells, a model of porcine proximal tubule cells, were used. ATP (10(-6)M) or Ado (10(-6)M) sp...

journal_title：Archives of biochemistry and biophysics

authors： Wengert M,Ribeiro MC,Abreu TP,Coutinho-Silva R,Leão-Ferreira LR,Pinheiro AA,Caruso-Neves C

更新日期：2013-07-15 00:00:00

abstract：:The fundamental improvement of muscle ischemia requires the re-establishment of sufficient vessel network. Despite many kinds of drugs have been used for ischemia, effective angiogenic drug is very limited. Here, we reported the identification and isolation of a potent angiogenic fraction (angio-T) from Geum japonicum...

journal_title：Archives of biochemistry and biophysics

authors： Cheung WL,Cheng L,Liu H,Gu X,Li M

更新日期：2007-03-01 00:00:00

abstract：:Triacylglycerol plays a critical role in an organism's ability to withstand fuel deprivation, and dysregulation of triacylglycerol synthesis is important in the development of diseases such as obesity and diabetes. Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial and committed step of glycerolipid syn...

journal_title：Archives of biochemistry and biophysics

authors： Lewin TM,Granger DA,Kim JH,Coleman RA

更新日期：2001-12-01 00:00:00

abstract：:An in vitro acrosome-like reaction was induced in spermatozoa from the boar cauda epididymis by incubation in Tyrode's solution containing 1 mg/ml fatty acid-free bovine serum albumin. Plasma membranes were isolated from the spermatozoa at different times during the incubation and analyzed for their lipid composition....

journal_title：Archives of biochemistry and biophysics

authors： Nikolopoulou M,Soucek DA,Vary JC

更新日期：1986-10-01 00:00:00

abstract：:The antioxidant, butylated hydroxytoluene (BHT), causes lung toxicity in mice followed by regenerative repair, and can also modulate the development of carcinogen-induced lung adenomas. We are investigating changes in pulmonary biochemistry following BHT treatment in order to understand the mechanisms of BHT-induced p...

journal_title：Archives of biochemistry and biophysics

authors： Blumenthal EJ,Malkinson AM

更新日期：1987-07-01 00:00:00

abstract：:Cadmium is toxic and carcinogenic to humans and animals. The testis and lung are the target organs for cadmium carcinogenesis. Heat shock proteins (HSPs) as well as metallothionein (MT) and glutathione (GSH) play an important role in protection against its toxicity. HSP32, also known as heme oxygenase-1, is a 32-kDa p...

journal_title：Archives of biochemistry and biophysics

authors： Abe T,Yamamoto O,Gotoh S,Yan Y,Todaka N,Higashi K

更新日期：2000-10-01 00:00:00

abstract：:This work addresses a correlation between the redox state of pyridine nucleotides and that of sulfhydryl groups of the mitochondrial membranes. Several major observations emerge: (1) Conditions leading to an oxidation of the pyridine nucleotides such as incubation with tert-butyl hydroperoxide or acetoacetate determin...

journal_title：Archives of biochemistry and biophysics

authors： Bindoli A,Callegaro MT,Barzon E,Benetti M,Rigobello MP

更新日期：1997-06-01 00:00:00

abstract：:Saccharopine dehydrogenase catalyzes the NAD-dependent oxidative deamination of saccharopine to l-lysine and α-ketoglutarate. Lysine 99 is within hydrogen-bond distance to the α-carboxylate of the lysine substrate and D319 is in the vicinity of the carboxamide side chain of NADH. Both are conserved and may be importan...

journal_title：Archives of biochemistry and biophysics

authors： Ekanayake DK,West AH,Cook PF

更新日期：2011-10-01 00:00:00

abstract：:The activity of thymidylate synthetase in the liver of the ddY strain male mouse increased transitorily according to the increase in tumor cell number at maximum 7-9 days after ip transplantation of Ehrlich ascites tumor. The enzyme was able to be purified from the tumor host mouse liver or from the normal mouse liver...

journal_title：Archives of biochemistry and biophysics

authors： Asanagi M,Moriyama Y,Fujimura S

更新日期：1988-12-01 00:00:00

abstract：:The G1 glycoprotein of Punta Toro virus, a member of the bunyavirus family, accumulates in the Golgi complex when it is expressed from cloned cDNA. We previously reported that the information necessary for Golgi retention of the G1 protein is located within the transmembrane domain and a portion of the cytoplasmic dom...

journal_title：Archives of biochemistry and biophysics

authors： Matsuoka Y,Chen SY,Holland CE,Compans RW

更新日期：1996-12-01 00:00:00

abstract：:Cellobiose dehydrogenase (CDH), an extracellular hemoflavoenzyme produced by the cellulose-degrading cultures of Phanerochaete chrysosporium, oxidizes cellobiose to cellobionolactone. CDH has been purified to homogeneity by a five-step purification procedure. The homogeneous CDH is monomeric and has a relative molecul...

journal_title：Archives of biochemistry and biophysics

authors： Bao W,Usha SN,Renganathan V

更新日期：1993-02-01 00:00:00

abstract：:Hepatocytes from fed rats were incubated for 120 min in the presence of alpha-D-[1,2-13C]glucose pentaacetate (1.7 mM), both D-[1,2-13C]glucose (1.7 mM) and acetate (8.5 mM), alpha-D-glucose penta[2-13C]acetate (1.7 mM), or D-[1,2-13C]glucose (8.3 mM). The amounts of 13C-enriched L-lactate and D-glucose and those of a...

journal_title：Archives of biochemistry and biophysics

authors： Malaisse WJ,Ladrière L,Kadiata MM,Verbruggen I,Willem R

更新日期：2000-09-01 00:00:00

abstract：:The reduction of prostaglandin H synthase compound II by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 M phosphate buffer (pH 8.0) at 4.0 +/- 0.5 degrees C, by rapid scan spectrometry and transient state kinetics. A saturation effect and nonzero intercept were observed in the plot of pseud...

journal_title：Archives of biochemistry and biophysics

authors： Hsuanyu Y,Dunford HB

更新日期：1990-09-01 00:00:00

abstract：:Maturation of the precursor forms of bovine cholesterol side-chain cleavage cytochrome P-450 (P-450SCC) and 11 beta-hydroxylase cytochrome P-450 (P-450(11)beta) was investigated using mitochondria from bovine corpus luteum. The results show that both precursors, whose synthesis was directed by bovine adrenocortical RN...

journal_title：Archives of biochemistry and biophysics

authors： Matocha MF,Waterman MR

更新日期：1986-11-01 00:00:00

abstract：:Electrophoretically homogeneous preparations of catalytic subunit (C) of cAMP-dependent protein kinase isolated according to two different procedures from holoenzyme type I and type II from rabbit and from holoenzyme type II from rat skeletal muscle and from bovine cardiac muscle can be separated on carboxymethyl cell...

journal_title：Archives of biochemistry and biophysics

authors： Kinzel V,Hotz A,König N,Gagelmann M,Pyerin W,Reed J,Kübler D,Hofmann F,Obst C,Gensheimer HP

更新日期：1987-03-01 00:00:00

abstract：:Experimental autoimmune encephalomyelitis (EAE) is a commonly used mouse model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelination leading to brain and spinal cord malfunctions. We postulate that not only biological but also biomechanical properties ...

journal_title：Archives of biochemistry and biophysics

authors： Pyka-Fościak G,Zemła J,Lis GJ,Litwin JA,Lekka M

更新日期：2020-02-15 00:00:00

abstract：:Nucleotide Binding Domains (NBDs) are responsible for the ATPase activity of the multidrug resistance protein 1 (MRP1). A series of NBD1-linker-NBD2 chimeric fusion proteins were constructed, expressed and purified, and their ATPase activities were analyzed. We report here that a GST linked NBD1(642-890)-GST-NBD2(1286...

journal_title：Archives of biochemistry and biophysics

authors： Wan L,Liang X,Huang Y

更新日期：2009-05-15 00:00:00

abstract：:The mouse myoblast cell line C2C12 constitutively expressed 160-kDa transmembrane NCAM isoform and 135-kDa GPI-anchored isoform before differentiation. During differentiation into multinucleated myotubes, the cells newly expressed 150-kDa GPI-anchored isoform and the level of 135-kDa GPI-anchored isoform increased. St...

journal_title：Archives of biochemistry and biophysics

authors： Mukasa R,Umeda M,Endo T,Kobata A,Inoue K

更新日期：1995-04-01 00:00:00

abstract：:The zinc-binding motif (HELLGH) of dipeptidyl peptidase III (DPP III) is different from the common zinc-binding motif (HExxH) of metallopeptidases. To clarify the importance of the zinc-binding motif part of DPP III for enzymatic activity, we measured the recovery of the enzyme activity of apo-Leu(453)-deleted dipepti...

journal_title：Archives of biochemistry and biophysics

authors： Hirose J,Kamigakiuchi H,Iwamoto H,Fujii H,Nakai M,Takenaka M,Kataoka R,Sugahara M,Yamamoto S,Fukasawa KM

更新日期：2004-11-01 00:00:00

abstract：:Cold plasma is partly ionized non-thermal plasma generated at atmospheric pressure. It has been recognized as an alternative approach in medicine for sterilization of wounds, promotion of wound healing, topical treatment of skin diseases with microbial involvement and treatment of cancer. Cold plasma used in wound the...

journal_title：Archives of biochemistry and biophysics

authors： Mohd Nasir N,Lee BK,Yap SS,Thong KL,Yap SL

更新日期：2016-09-01 00:00:00

abstract：:The dipeptidyl peptidase IV (DP IV, EC 3.4.14.5) gene from Flavobacterium meningosepticum was cloned by Southern and colony hybridizations using probes amplified by PCR, and expressed in Escherichia coli DH1. E. coli DH1 harboring pFDP-H1, which was a subclone derived from the positive clone pFDP-1, showed 3.5-fold hi...

journal_title：Archives of biochemistry and biophysics

authors： Kabashima T,Yoshida T,Ito K,Yoshimoto T

更新日期：1995-06-20 00:00:00

abstract：:While molecular adaptation to high temperature has been extensively studied, the effect of hydrostatic pressure on protein structure and enzymatic activity is still poorly understood. We have studied the influence of pressure on both the quaternary structure and enzymatic activity of the dodecameric TET3 peptidase fro...

journal_title：Archives of biochemistry and biophysics

authors： Rosenbaum E,Gabel F,Durá MA,Finet S,Cléry-Barraud C,Masson P,Franzetti B

更新日期：2012-01-15 00:00:00

abstract：:Kinetic analysis has shown that dimethylnitrosamine, dipropylnitrosamine, dibutylnitrosamine, and diphenylnitrosamine initially act as reversible competitive inhibitors with respect to the substrate, acetylthiocholine chloride. The inhibitor constants Ki vary from 21-30 microM for the aliphatic nitrosamines to 8.2 mic...

journal_title：Archives of biochemistry and biophysics

authors： Shuttleworth B,de Villiers DL,Whiteley CG

更新日期：1990-06-01 00:00:00

abstract：:To establish a cell line expressing enhanced levels of beta-amyloid precursor protein (beta-APP), we constructed plasmid DNAs expressing beta-APP-751 mRNA and transfected them into COS-1 cells. Using a modified version of the reverse transcriptase polymerase chain reaction which is RNA sensitive to study the beta-APP ...

journal_title：Archives of biochemistry and biophysics

authors： Ramakrishna N,Smedman M,Gillam B

更新日期：1996-02-15 00:00:00