Androgen receptor-mediated regulation of the anti-atherogenic enzyme CYP27A1 involves the JNK/c-jun pathway.


:CYP27A1, an enzyme with several important roles in cholesterol homeostasis and vitamin D₃ metabolism, has been ascribed anti-atherogenic properties. This study addresses an important problem regarding how this enzyme, involved in cholesterol metabolism in the liver and peripheral tissues, is regulated. Our results identify the human CYP27A1 gene as a new target for the JNK/c-jun pathway. Initial experiments showed that an inhibitor of c-Jun N-terminal kinase (JNK) downregulated basal CYP27A1 promoter activity whereas overexpression of JNK slightly enhanced promoter activity. Androgen receptor (AR)-mediated upregulation of mRNA levels and endogenous enzyme activity was recently reported. In the present study, the AR antagonist nilutamide blocked the androgen induction of CYP27A1. The present data revealed that inhibition of the JNK/c-jun pathway abolishes the AR-mediated effect on CYP27A1 transcription and enzyme activity, whereas overexpression of JNK markedly increased androgenic upregulation of CYP27A1. In conclusion, the current results indicate involvement of the JNK/c-jun pathway in AR-mediated upregulation of human CYP27A1. The link to JNK signaling is interesting since inflammatory processes may upregulate CYP27A1 to clear cholesterol from peripheral tissues.


Arch Biochem Biophys


Norlin M,Pettersson H,Tang W,Wikvall K




Has Abstract


2011-02-15 00:00:00














  • Secretion of a nonspecific lipid transfer protein by hepatoma cells in culture.

    abstract::Nonspecific lipid transfer protein (sterol carrier protein2) has previously been proposed to function as (i) a catalyst for intracellular movement of newly synthesized phospholipid, (ii) a cofactor in the biosynthesis and metabolism of cholesterol, and (iii) a cofactor in the feedback inhibition of cholesterol synthes...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Crain RC,Clark RW

    更新日期:1985-08-15 00:00:00

  • Characterization of Ceriporiopsis subvermispora bicupin oxalate oxidase expressed in Pichia pastoris.

    abstract::Oxalate oxidase (E.C. catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction that is coupled with the formation of hydrogen peroxide. Although there is currently no structural information available for oxalate oxidase from Ceriporiopsis subvermispora (CsOxOx), sequence data and ho...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Moussatche P,Angerhofer A,Imaram W,Hoffer E,Uberto K,Brooks C,Bruce C,Sledge D,Richards NG,Moomaw EW

    更新日期:2011-05-01 00:00:00

  • Biophysical and biochemical studies on glycoxidatively modified human low density lipoprotein.

    abstract::Methylglyoxal (MGO), a reactive dicarbonyl metabolite is a potent arginine directed glycating agent which has implications for diabetes-related complications. Dicarbonyl metabolites are produced endogenously and in a state of misbalance, they contribute to cell and tissue dysfunction through protein and DNA modificati...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Abidi M,Khan MS,Ahmad S,Kausar T,Nayeem SM,Islam S,Ali A,Alam K,Moinuddin

    更新日期:2018-05-01 00:00:00

  • Quantification of cysteine sulfinic acid decarboxylase in male and female rats: effect of adrenalectomy and methionine.

    abstract::Hepatic cysteine sulfinic acid decarboxylase (EC activity has been reported to decrease in response to both L-methionine (Met) feeding and adrenalectomy in rats. A series of experiments was conducted to (a) determine if CSAD depression was evident in female rats fed a methionine-supplemented diet; and (b) de...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jerkins AA,Steele RD

    更新日期:1992-05-01 00:00:00

  • Reaction of ozone with glycophorin in solution and in lipid vesicles.

    abstract::Glycophorin from human red blood cells was exposed to ozone in aqueous solution. Amino acid analysis of glycophorin exposed to a 10-fold molar excess of ozone showed that the only residue affected was methionine. Both methionine residues of the protein were oxidized to methionine sulfoxide. Exposure of the oxidized pr...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Banerjee SK,Mudd JB

    更新日期:1992-05-15 00:00:00

  • Expression profile and interactions of hnRNP A3 within hnRNP/mRNP complexes in mammals.

    abstract::The hnRNP A/B family contains abundant nuclear proteins with major roles in alternative splicing and the ability for nucleo-cytoplasmic shuttling. Compared to the best known members of this family (hnRNP A1, A2/B1), hnRNP A3 is a relatively less known protein. We report herein immunochemical studies with the hnRNP A3 ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Papadopoulou C,Boukakis G,Ganou V,Patrinou-Georgoula M,Guialis A

    更新日期:2012-07-15 00:00:00

  • A study of human furin specificity using synthetic peptides derived from natural substrates, and effects of potassium ions.

    abstract::We explored furin substrate requirements in addition to the motif R-X-K/R-R using synthetic fluorescent resonance energy transfer (FRET) decapeptides. These decapeptides were derived from furin cleavage sites in viral coat glycoproteins and human and bacterial protein precursors. The hydrolysis by furin of most substr...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Izidoro MA,Gouvea IE,Santos JA,Assis DM,Oliveira V,Judice WA,Juliano MA,Lindberg I,Juliano L

    更新日期:2009-07-15 00:00:00

  • Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.

    abstract::The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions. Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation. To understand ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shahid S,Ahmad F,Hassan MI,Islam A

    更新日期:2015-10-15 00:00:00

  • Galactosylsphingosine inhibition of the broad-specificity cytosolic beta-glucosidase of human liver.

    abstract::Glucosylsphingosine is a potent inhibitor of lysosomal glucocerebrosidase and the broad-specificity, cytosolic beta-glucosidase of human liver. In the present study, it was demonstrated that the broad-specificity beta-glucosidase was also inhibited by galactosylsphingosine. The inhibition was observed when the enzyme ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: LaMarco KL,Glew RH

    更新日期:1985-02-01 00:00:00

  • Age- and sex-related expression of cytochromes p450f and P450g in rat liver.

    abstract::We have previously shown that rat hepatic cytochromes P450f, P450g, P450h, and P450i possess a high degree of immunochemical and, presumably, structural relatedness. Polyclonal antibodies directed against cytochromes P450f and P450g were made monospecific by immunoabsorption against the cross-reactive proteins. The sp...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Bandiera S,Ryan DE,Levin W,Thomas PE

    更新日期:1986-08-01 00:00:00

  • Retinoids inhibit the oxidative modification of protein kinase C induced by oxidant tumor promoters.

    abstract::Recently we reported that oxidant tumor promoters can induce the oxidative modification of protein kinase C (PKC) resulting in either activation or inactivation of the kinase (R. Gopalakrishna and W. B. Anderson, Arch. Biochem. Biophys. 285, 382-387, 1991). Since retinoids previously have been shown to antagonize the ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gundimeda U,Hara SK,Anderson WB,Gopalakrishna R

    更新日期:1993-01-01 00:00:00

  • Activation of calcium-sensing receptor by allosteric agonists cinacalcet and AC-265347 abolishes the 1,25(OH)2D3-induced Ca2+ transport: Evidence that explains how the intestine prevents excessive Ca2+ absorption.

    abstract::Long-term high-calcium intake and intestinal calcium hyperabsorption are hazardous to the body. It is hypothesized that enterocytes possess mechanisms for preventing superfluous calcium absorption, including secretion of negative regulators of calcium absorption and utilization of calcium-sensing receptor (CaSR) to de...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wongdee K,Rodrat M,Keadsai C,Jantarajit W,Teerapornpuntakit J,Thongbunchoo J,Charoenphandhu N

    更新日期:2018-11-01 00:00:00

  • The same xenobiotic response element is required for constitutive and inducible expression of the mammalian aldehyde dehydrogenase-3 gene.

    abstract::The mammalian aldehyde dehydrogenase-3 gene (ALDH3) exhibits several aspects of tissue-specific expression. Certain normal tissues, such as the cornea, constitutively express ALDH3 at very high levels. Other tissues, such as normal liver, do not express ALDH3. In liver, ALDH3 is inducible by polycyclic aromatic hydroc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Boesch JS,Miskimins R,Miskimins WK,Lindahl R

    更新日期:1999-01-15 00:00:00

  • Alteration of pyruvate metabolism in African trypanosomes during differentiation from bloodstream into insect forms.

    abstract::In the presence of glucose and ample oxygen, insect form African trypanosomes release pyruvate more than 100-fold more slowly than do bloodstream forms. This rate decrease could not be accounted for simply by an increased mitochondrial pyruvate oxidation rate as inhibiting mitochondrial respiration increases pyruvate ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Barnard JP,Pedersen PL

    更新日期:1994-08-15 00:00:00

  • A novel variant of mouse MATE-1 H+/organic cation antiporter with a long hydrophobic tail.

    abstract::Mammalian multidrug and toxic compound extrusion 1 (MATE1) are polyspecific H+-coupled exporters of organic cations (OCs) and responsible for excretion of metabolic waste products and xenobiotics. Here, we report a novel variant of mouse MATE1, mMATE1b, that has a long carboxyl terminal hydrophobic tail homologous to ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kobara A,Hiasa M,Matsumoto T,Otsuka M,Omote H,Moriyama Y

    更新日期:2008-01-15 00:00:00

  • The amino-terminal region of the long-chain fatty acid transport protein FadL contains an externally exposed domain required for bacteriophage T2 binding.

    abstract::The fatty acid transport protein FadL from Escherichia coli is predicted to be rich in beta-structure and span the outer membrane multiple times to form a long-chain fatty acid specific channel. Proteolysis of FadL within whole cells, total membranes, and isolated outer membranes identified two trypsin-sensitive sites...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Cristalli G,DiRusso CC,Black PN

    更新日期:2000-05-15 00:00:00

  • Factors influencing pyrroline 5-carboxylate synthesis from glutamate by rat intestinal mucosa mitochondria.

    abstract::Factors influencing pyrroline 5-carboxylate (P5C) synthesis from glutamate by a subcellular fraction enriched in mitochondria of rat small intestinal mucosa have been studied. P5C synthesis decreased rapidly if this subcellular fraction was preincubated at 20 degrees C in the absence of substrates; this effect suggest...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Henslee JG,Wakabayashi Y,Small C,Jones ME

    更新日期:1983-10-15 00:00:00

  • Regulation of intestinal calbindin-D28K gene expression: a solution hybridization study.

    abstract::A solution hybridization assay employing specific synthetic oligodeoxynucleotide probes was developed to study the regulation of intestinal calbindin-D28K mRNA. The technique is rapid and quantitative and eliminates the need for sample transfer, blotting, autoradiography, and densitometry. Following validation of the ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Fullmer CS

    更新日期:1990-11-15 00:00:00

  • Interleukin-2 is one of the targets of 1,25-dihydroxyvitamin D3 in the immune system.

    abstract::Interleukin (IL)-2 knockout (KO) mice, which spontaneously develop symptoms of inflammatory bowel disease similar to ulcerative colitis in humans, were made vitamin D deficient (D-) or vitamin D sufficient (D+) or were supplemented with 1,25-dihydroxyvitamin D(3) (1,25D3). 1,25-Dihydroxyvitamin D3 supplementation, but...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Bemiss CJ,Mahon BD,Henry A,Weaver V,Cantorna MT

    更新日期:2002-06-15 00:00:00

  • Conditional regulation of the human CYP4X1 and CYP4Z1 genes.

    abstract::Cytochrome P450 genes (CYPs) encoding two new subfamilies designated CYP4X1 and CYP4Z1 were identified in the human genome and the Expressed Sequence Tags database. Partial cDNAs encoding both P450s were isolated from human kidney and used to determine tissue distribution. CYP4X1 was predominantly expressed in trachea...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Savas U,Hsu MH,Griffin KJ,Bell DR,Johnson EF

    更新日期:2005-04-15 00:00:00

  • Synthesis and properties of adenosine-5'-triphosphoro-gamma-1-(5-sulfonic acid)naphthyl ethylamidate: a fluorescent nucleotide substrate for DNA-dependent RNA polymerase from Escherichia coli.

    abstract::A new fluorescent ATP analog, adenosine-5'-triphosphoro-gamma-1-(5-sulfonic acid)naphthyl ethylamidate (gamma-1,5-EDANS)ATP, containing the fluorophore N-(aminoethyl)-5-naphthylamine-1-sulfonic acid attached via a gamma-phosphoamidate bond was synthesized in good yield. It has absorption maxima at 255 and 344 nm and a...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wu FY,Abdulwajid AW,Solaiman D

    更新日期:1986-05-01 00:00:00

  • The effect of Co(III)(NH3)4ATP on the kinetics of beef heart mitochondrial ATPase.

    abstract::Bidentate cobalt(III)tetraamine adenosine triphosphate [Co(NH3)4ATP] was investigated as an inhibitor of the beef heart mitochondrial F1-ATPase. The compound was found to have a mixed noncompetitive mechanism with a Ki of 0.4 mM and an alpha of 1.4 during ATP hydrolysis. Co(NH3)4ATP also noncompetitively inhibited ATP...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Steinke L,Schuster SM

    更新日期:1985-05-01 00:00:00

  • Acetone-inducible cytochrome P-450: purification, catalytic activity, and interaction with cytochrome b5.

    abstract::A procedure was developed for the purification of an acetone-inducible form of cytochrome P-450 (P-450ac) to electrophoretical homogeneity from liver microsomes of acetone-treated rats. The P-450ac preparation containing 16.0 to 16.5 nmol P-450/mg protein moved as a single protein band with an estimated molecular weig...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Patten CJ,Ning SM,Lu AY,Yang CS

    更新日期:1986-12-01 00:00:00

  • Mechanism of simultaneous iodination and coupling catalyzed by thyroid peroxidase.

    abstract::Thyroid peroxidase (TPO) simultaneously catalyzes two very different types of reaction in the thyroid gland- iodination and coupling. The present study addresses the mechanism of this simultaneous dual activity. Compound I, the two-electron oxidation product of TPO, exists in two different forms--an oxoferryl porphyri...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Taurog A,Dorris ML,Doerge DR

    更新日期:1996-06-01 00:00:00

  • Molecular diversity of calpastatin in human erythroid cells.

    abstract::According to differences in mobility on SDS-polyacrylamide gel electrophoresis, calpastatins (inhibitor proteins of the calcium-dependent proteinase calpain) are classified into the tissue type (100-120 kDa) and the erythrocyte type (70 kDa), which lacks the amino-terminal domains (domains L and 1). We investigated th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Takano E,Nosaka T,Lee WJ,Nakamura K,Takahashi T,Funaki M,Okada H,Hatanaka M,Maki M

    更新日期:1993-06-01 00:00:00

  • Effects of lipids on the interaction of SecA with model membranes.

    abstract::The effects of nonlamellar-prone lipids, diacylglycerol and phosphatidylethanolamine (PE), on the kinetic association of SecA with model membranes were examined by measuring changes in the intrinsic emission fluorescence with a stopped-flow apparatus. Upon interaction with standard liposomes composed of 50 mol% dioleo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ahn T,Kim JS,Lee BC,Yun CH

    更新日期:2001-11-01 00:00:00

  • Resonance Raman spectroscopic evaluation of skin carotenoids as a biomarker of carotenoid status for human studies.

    abstract::Resonance Raman spectroscopy (RRS) is a non-invasive method that has been developed to assess carotenoid status in human tissues including human skin in vivo. Skin carotenoid status has been suggested as a promising biomarker for human studies. This manuscript describes research done relevant to the development of thi...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Mayne ST,Cartmel B,Scarmo S,Jahns L,Ermakov IV,Gellermann W

    更新日期:2013-11-15 00:00:00

  • Synaptic plasma membrane structure and polarity of long-sleep and short-sleep mice.

    abstract::Membrane dielectric as a primary basis for effects of ethanol was examined in synaptic plasma membranes (SPM) of genetically selected ethanol-sensitive long-sleep (LS) and ethanol-resistant short-sleep (SS) mice. Multifrequency phase and modulation of fluorometry of diphenylhexatriene (DPH) was used to resolve structu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Schroeder F,Colles SM,Kreishman GP,Heyliger CE,Wood WG

    更新日期:1994-03-01 00:00:00

  • Isolation and biochemical characterization of hemorrhagic toxin f from the venom of Crotalus atrox (western diamondback rattlesnake).

    abstract::Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

    更新日期:1984-06-01 00:00:00

  • Aggregation and proton release of purple and white membranes following cleavage of the carboxyl-terminal tail of bacteriorhodopsin.

    abstract::Our results indicate that the previously reported decrease in proton release by proteolyzed purple membrane sheets was due merely to the aggregation state of these preparations and not to the loss of the carboxyl-terminal tail. Changes in H+/M412 ratios obtained for purple and white membrane preparations correlate wit...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Arrio B,Johannin G,Volfin P,Lefort-Tran M,Packer L,Robinson AE,Hrabeta E

    更新日期:1986-04-01 00:00:00