Both PA63 and PA83 are endocytosed within an anthrax protective antigen mixed heptamer: a putative mechanism to overcome a furin deficiency.

abstract：:Peribacteroid membranes can be isolated in essentially pure form from 20-day lupin root nodules by osmotic shock of the purified membrane enclosed bacteroids. The ATPase (EC 3.6.1.3) associated with this membrane has an acid pH optimum (5.25) and is specific for ATP (Mg-ATP Km = 0.16 mM). The enzyme activity requires ...

journal_title：Archives of biochemistry and biophysics

authors： Domigan NM,Farnden KJ,Robertson JG,Monk BC

更新日期：1988-08-01 00:00:00

abstract：:A preliminary study (J.M. Mata, R. Assad, and B. Peterkofsky (1981) Arch. Biochem. Biophys. 206, 93-104) suggested that chick embryo limb bone microsomes took up and concentrated [14C]ascorbate in the presence of cofactors for prolyl hydroxylase. In the present study, we found that the apparent Km for ascorbate in the...

journal_title：Archives of biochemistry and biophysics

authors： Peterkofsky B,Tschank G,Luedke C

更新日期：1987-04-01 00:00:00

abstract：:The presence of a trifunctional beta-oxidation complex in pig heart and its relationship to the known long-chain enoyl-CoA hydratase (EC 4.2.1.74) from pig heart mitochondria were investigated. For this study, the complex was partially purified by chromatography on DEAE-cellulose in the absence of detergents and was p...

journal_title：Archives of biochemistry and biophysics

authors： Luo MJ,He XY,Sprecher H,Schulz H

更新日期：1993-07-01 00:00:00

abstract：:Studies of modification of hemoglobin and of sickle hemoglobin by alternative aspirins have been extended to a series of new bis esters with a variety of substituted bridging diacids and to a group of mono esters with polar acyl groups. Rates of hydrolysis of these alternative aspirins have also been examined, and the...

journal_title：Archives of biochemistry and biophysics

authors： Delaney EJ,Massil SE,Shi GY,Klotz IM

更新日期：1984-02-01 00:00:00

abstract：:Recently our group synthesized a new class of melanins obtained by the tyrosinase-catalyzed oxidation of opioid peptides (opiomelanins). Owing to the presence of the peptide moiety such pigments exhibit high solubility in hydrophilic solvents, which allows spectroscopic investigations. In particular, the absence of so...

journal_title：Archives of biochemistry and biophysics

authors： Mosca L,De Marco C,Fontana M,Rosei MA

更新日期：1999-11-01 00:00:00

abstract：:We have developed derivatives of mouse embryonic fibroblasts (10T1/2) and Chinese hamster ovary (AS52) cells that stably express high levels of murine prostaglandin synthase-1 or -2 (PGHS-1 or -2). The cDNAs were transferred using retroviral vectors and the resulting G418-resistant clones were analyzed for prostagland...

journal_title：Archives of biochemistry and biophysics

authors： Chulada PC,Loftin CD,Winn VD,Young DA,Tiano HF,Eling TE,Langenbach R

更新日期：1996-06-15 00:00:00

abstract：:Plasminogen activator inhibitor (PAI)-1 is a major fibrinolytic inhibitor. High PAI-1 is associated with increased renal and cardiovascular disease risk. Previous studies demonstrated PAI-1 down-regulation by 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃), but the molecular mechanism remains unknown. Here we show that exposur...

journal_title：Archives of biochemistry and biophysics

authors： Chen Y,Kong J,Sun T,Li G,Szeto FL,Liu W,Deb DK,Wang Y,Zhao Q,Thadhani R,Li YC

更新日期：2011-03-15 00:00:00

abstract：:The relative rates of transcription of the rat liver glutathione S-transferase Ya-Yc and Yb genes were determined in purified liver nuclei isolated at different times after phenobarbital or 3-methylcholanthrene administration. The transcriptional rates of the Ya-Yc and Yb genes were elevated approximately fivefold 8 a...

journal_title：Archives of biochemistry and biophysics

authors： Ding VD,Pickett CB

更新日期：1985-08-01 00:00:00

abstract：:Nonspecific lipid transfer protein (sterol carrier protein2) has previously been proposed to function as (i) a catalyst for intracellular movement of newly synthesized phospholipid, (ii) a cofactor in the biosynthesis and metabolism of cholesterol, and (iii) a cofactor in the feedback inhibition of cholesterol synthes...

journal_title：Archives of biochemistry and biophysics

authors： Crain RC,Clark RW

更新日期：1985-08-15 00:00:00

abstract：:Erythropoietin was purified to homogeneity from the culture medium of a baby hamster kidney cell line stably transfected with a human erythropoietin gene. A three-step procedure was used, which included affinity chromatography, ion-exchange chromatography, and reverse-phase chromatography. Purity of the protein was co...

journal_title：Archives of biochemistry and biophysics

authors： Broudy VC,Tait JF,Powell JS

更新日期：1988-09-01 00:00:00

abstract：:P-815 mouse mastocytoma cells express the K isozyme of pyruvate kinase and the specific activity of this enzyme is increased in response to N6,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate, 8-bromoadenosine 3':5'-cyclic monophosphate, cholera toxin, and epinephrine, all of which also elevate the intracellular con...

journal_title：Archives of biochemistry and biophysics

authors： Ngo JL,Ibsen KH

更新日期：1989-03-01 00:00:00

abstract：:The formation of alpha-hydroxyethyl radical from ethanol by deermouse microsomes supplemented with NADPH has been demonstrated with the EPR technique of spin trapping with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN) as the spin trap, in the presence of deferoxamine mesylate. Superoxide dismutase prevented the fo...

journal_title：Archives of biochemistry and biophysics

authors： Knecht KT,Thurman RG,Mason RP

更新日期：1993-06-01 00:00:00

abstract：:Myeloperoxidase (MPO) is expressed by myeloid cells for the purpose of catalyzing the formation of hypochlorous acid, from chloride ions and reaction with a hydrogen peroxide-charged heme covalently bound to the enzyme. Most peroxidase enzymes both plant and mammalian are inhibited by benzoic acid hydrazide (BAH)-cont...

journal_title：Archives of biochemistry and biophysics

authors： Huang J,Smith F,Panizzi JR,Goodwin DC,Panizzi P

更新日期：2015-03-15 00:00:00

abstract：:The importance of the sn-glycerol- 3-phosphate (G-3-P) electron transfer shuttle in hormonal regulation of gluconeogenesis was examined in hepatocytes from rats with decreased mitochondrial G-3-P dehydrogenase activity (thyroidectomized) or increased G-3-P dehydrogenase activity [triiodothyronine (T(3)) or dehydroepia...

journal_title：Archives of biochemistry and biophysics

authors： Kneer N,Lardy H

更新日期：2000-03-01 00:00:00

abstract：BACKGROUND:The main functions of the skin are to protect against environmental insults and prevent water loss, which are performed by the complex lipid- and protein matrix present in the outermost layers of the epithelium. The lipidome of these outer layers is mainly composed of ceramides, fatty acids, and cholesterol,...

journal_title：Archives of biochemistry and biophysics

authors： Muresan XM,Narzt MS,Woodby B,Ferrara F,Gruber F,Valacchi G

更新日期：2019-05-15 00:00:00

abstract：:The expression and activity of the phenobarbital (PB)-inducible P-450 isozymes, P-450b and P-450e, and the major 3-methylcholanthrene (MC)-inducible form, P-450c, were studied in primary cultures of adult rat hepatocytes in T1, Leibovitz L-15 (L-15), and a modification of Waymouth 752/1 (Way) media. P-450 isozymes in ...

journal_title：Archives of biochemistry and biophysics

authors： Turner NA,Wilson NM,Jefcoate CR,Pitot HC

更新日期：1988-05-15 00:00:00

abstract：:G. metallireducens bacterium has highly versatile respiratory pathways that provide the microorganism an enormous potential for many biotechnological applications. However, little is known about the structural and functional properties of its electron transfer components. In this work, the periplasmic cytochrome PpcA ...

journal_title：Archives of biochemistry and biophysics

authors： Portela PC,Fernandes TM,Dantas JM,Ferreira MR,Salgueiro CA

更新日期：2018-04-15 00:00:00

abstract：:The kinetic analysis of the enzymatic reaction catalyzed by glycogen phosphorylase b from the rabbit skeletal muscle has been carried out in the direction of glycogen synthesis under the conditions of the saturation of the enzyme by glycogen. The applicability of 12 different variants of the Monod-Wyman-Changeux model...

journal_title：Archives of biochemistry and biophysics

authors： Klinov SV,Kurganov BI

更新日期：1994-07-01 00:00:00

abstract：:Cell extracts of Methanobacterium thermoautotrophicum possess a methenyl-tetrahydromethanopterin (methenyl-H4MPT) cyclohydrolase. The enzyme catalyzes the hydrolysis of methenyl-H4MPT to formyltetrahydromethanopterin (formyl-H4MPT). The reaction is reversible and both the rate and extent of the reaction depend on the ...

journal_title：Archives of biochemistry and biophysics

authors： Donnelly MI,Escalante-Semerena JC,Rinehart KL Jr,Wolfe RS

更新日期：1985-11-01 00:00:00

abstract：:Cytochrome b5 reductase (cb5r), a member of the flavoprotein transhydrogenase family of oxidoreductase enzymes, catalyzes the transfer of reducing equivalents from the physiological electron donor, NADH, to two molecules of cytochrome b5. We have determined the correct nucleotide sequence for the putative full-length,...

journal_title：Archives of biochemistry and biophysics

authors： Roma GW,Crowley LJ,Barber MJ

更新日期：2006-08-01 00:00:00

abstract：:Under physiological conditions the uptake of transferrin-bound iron by reticulocytes involves transferrin binding to membrane receptors followed by endocytosis, release of iron from the transferrin within endosomes, and recycling of apotransferrin to the cell surface. However, as shown in the present work, iron uptake...

journal_title：Archives of biochemistry and biophysics

authors： Hodgson LL,Quail EA,Morgan EH

更新日期：1994-01-01 00:00:00

abstract：:Glycerol is a dietary component which is metabolized primarily by the liver and kidney where it is used mainly for glucose synthesis. The metabolism of glycerol is very similar to that of dihydroxyacetone which can be considered its more oxidized counterpart. The effects of these substrates on hepatic lipogenesis and ...

journal_title：Archives of biochemistry and biophysics

authors： Carmona A,Freedland RA

更新日期：1989-05-15 00:00:00

abstract：:Three alanine aminotransferases, two minor (AlaAT-1, AlaAT-3) and one major (AlaAT-2), were detected by native gel electrophoresis of leaf extracts from Panicum miliaceum L. AlaAT-2 was purified to homogeneity and a specific polyclonal antibody was raised against it which did not react with the other two forms of the ...

journal_title：Archives of biochemistry and biophysics

authors： Son D,Jo J,Sugiyama T

更新日期：1991-09-01 00:00:00

abstract：:Raspberries are a rich source of ellagitannins and anthocyanins. The aim of this work was to investigate whether raspberry consumption can improve vascular function and to understand which phenolic metabolites may be responsible for the effects. A 3 arm double-blind randomized controlled crossover human intervention t...

journal_title：Archives of biochemistry and biophysics

authors： Istas G,Feliciano RP,Weber T,Garcia-Villalba R,Tomas-Barberan F,Heiss C,Rodriguez-Mateos A

更新日期：2018-08-01 00:00:00

abstract：:Three distinct acid phosphatases were recently reported in avian breast muscle [J. H. Baxter and C. H. Suelter (1984) Arch. Biochem. Biophys. 228, 397-406]. Of the increased acid phosphatase activity in dystrophic muscle compared to normal muscle, 84% can be accounted for as a low-molecular-weight, cytosolic form. Thi...

journal_title：Archives of biochemistry and biophysics

authors： Baxter JH,Suelter CH

更新日期：1985-05-15 00:00:00

abstract：:A microsomal preparation from seedlings of Zea mays catalyzed the NAD+-dependent oxidative decarboxylation of several substrates, including 4alpha-carboxy-cholest-7-en-3beta-ol, synthesized according to a new procedure, giving the first in vitro evidence for this enzymatic activity in a higher plant. A GC assay has be...

journal_title：Archives of biochemistry and biophysics

authors： Rondet S,Taton M,Rahier A

更新日期：1999-06-15 00:00:00

abstract：:From mistletoe Viscum album L. extracts three chitin-binding lectin isoforms, cbML1, cbML2, and cbML3, were isolated and their primary structure determined. All three cbML isoforms are composed of two protein chains of 48 or 49 amino acid residues, linked by an intermolecular disulfide bond. The sequence of each singl...

journal_title：Archives of biochemistry and biophysics

authors： Stoeva S,Franz M,Wacker R,Krauspenhaar R,Guthöhrlein E,Mikhailov A,Betzel C,Voelter W

更新日期：2001-08-01 00:00:00

abstract：:The retinal pigment epithelium (RPE)-specific protein RPE65 is the major protein of the RPE microsomal membrane fraction. Though RPE65 lacks transmembrane domains or signal peptide, detergents are required for its maximally effective solubilization in isotonic buffers. However, in 0.75-1.0 M KCl, RPE65 is as soluble w...

journal_title：Archives of biochemistry and biophysics

authors： Tsilou E,Hamel CP,Yu S,Redmond TM

更新日期：1997-10-01 00:00:00

abstract：:Two forms of the beta-glucosidase amygdalin hydrolase (AH I and II), which catalyze the hydrolysis of (R)-amygdalin to (R)-prunasin and D-glucose, have been purified over 200-fold from mature black cherry (Prunus serotina Ehrh.) seeds. These proteins showed very similar molecular and kinetic properties but could be re...

journal_title：Archives of biochemistry and biophysics

authors： Kuroki GW,Poulton JE

更新日期：1986-06-01 00:00:00

abstract：:Histidinol dehydrogenase (EC 1.1.1.23) activity was determined in several plant species and in cultured plant cell lines. The enzyme was purified from cabbage (Brassica oleracea) to apparent homogeneity. To render complete purification, a new, specific histidinol-Sepharose 4B affinity chromatography was developed. The...

journal_title：Archives of biochemistry and biophysics

authors： Nagai A,Scheidegger A

更新日期：1991-01-01 00:00:00