当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Purification and characterization of the sesquiterpene cyclase aristolochene synthase from Penicillium roqueforti.

Purification and characterization of the sesquiterpene cyclase aristolochene synthase from Penicillium roqueforti.

Abstract：

:The sesquiterpene cyclase, aristolochene synthase, has been purified from Penicillium roqueforti by gel filtration and anion-exchange chromatography. Isolation was facilitated by a change in the elution behavior of the enzyme during gel filtration at different steps in the purification. The purified enzyme had a specific activity of 70 nmol/min/mg protein. The molecular weight as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was Mr 37,000. The native molecular weight as determined by gel filtration chromatography was Mr 48,000. The requirement for Mg2+ could be partially substituted with 0.01 mM Mn2+, but higher concentrations were inhibitory. Pyrophosphate, a competitive inhibitor of most terpene cyclases, had no effect on enzyme activity up to a concentration of 5.0 mM. The maximum activity was observed between pH 6.25 and pH 7.50, and the Km for farnesyl pyrophosphate was 0.55 +/- 0.06 microM.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Hohn TM,Plattner RD

doi

10.1016/0003-9861(89)90204-x

subject

Has Abstract

pub_date

1989-07-01 00:00:00

pages

137-43

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(89)90204-X

journal_volume

272

pub_type

杂志文章

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