Catalytic activity of cytochrome P4501A2 in reconstituted system with Emulgen 913.

abstract：:The dependence of respiratory flux via the alternative pathway on the redox poise of the ubiquinone (Q) pool was investigated in soybean cotyledon mitochondria. A marked nonlinear relationship was observed between Q-pool reduction level and O2 uptake via the alternative oxidase. Significant engagement of the alternati...

journal_title：Archives of biochemistry and biophysics

authors： Dry IB,Moore AL,Day DA,Wiskich JT

更新日期：1989-08-15 00:00:00

abstract：:Sesquiterpene cyclases (synthases) catalyze the conversion of the isoprenoid intermediate farnesyl diphosphate to various sesquiterpene structural types. In plants, many sesquiterpenes are produced as defensive chemicals (phytoalexins) or mediators of chemical communication (i.e., pollinator attractants). A number of ...

journal_title：Archives of biochemistry and biophysics

authors： Mercke P,Crock J,Croteau R,Brodelius PE

更新日期：1999-09-15 00:00:00

abstract：:Stationary phase inocula were more susceptible to the growth inhibitory effect of paraquat than were log phase inocula and this difference was exacerbated in strains overproducing superoxide dismutases (SOD). Glucose-6-phosphate dehydrogenase (G-6-PD), a member of the soxR regulon, was induced by paraquat promptly in ...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：1992-04-01 00:00:00

abstract：:Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in...

journal_title：Archives of biochemistry and biophysics

authors： Winnard PT,Esmon CT,Laue TM

更新日期：1989-02-15 00:00:00

abstract：:The flavoenzyme dihydroorotate dehydrogenase catalyzes the stereoselective oxidation of (S)-dihydroorotate to orotate in the fourth of the six conserved enzymatic reactions involved in the de novo pyrimidine biosynthetic pathway. Inhibition of pyrimidine metabolism by selectively targeting DHODHs has been exploited in...

journal_title：Archives of biochemistry and biophysics

authors： Reis RAG,Calil FA,Feliciano PR,Pinheiro MP,Nonato MC

更新日期：2017-10-15 00:00:00

abstract：:In the presence of glucose and ample oxygen, insect form African trypanosomes release pyruvate more than 100-fold more slowly than do bloodstream forms. This rate decrease could not be accounted for simply by an increased mitochondrial pyruvate oxidation rate as inhibiting mitochondrial respiration increases pyruvate ...

journal_title：Archives of biochemistry and biophysics

authors： Barnard JP,Pedersen PL

更新日期：1994-08-15 00:00:00

abstract：:Phagocytes generate superoxide (O2-.) and hydrogen peroxide (H2O2) and their interaction in an iron-catalyzed reaction to form hydroxyl radicals (OH.) (Haber-Weiss reaction) has been proposed. Deferoxamine chelates iron in a catalytically inactive form, and thus inhibition by deferoxamine has been employed as evidence...

journal_title：Archives of biochemistry and biophysics

authors： Klebanoff SJ,Waltersdorph AM

更新日期：1988-08-01 00:00:00

abstract：:The isolation and purification of a 21,000-Da (pI 4.9) novel protein from porcine anterior pituitary and whole human pituitary is described. Comparison of the NH2-terminal sequence of the first 77 and 81 residues of the human and porcine homologs shows only one conservative substitution at residue 12, namely an Ala fo...

journal_title：Archives of biochemistry and biophysics

authors： Seidah NG,Hsi KL,De Serres G,Rochemont J,Hamelin J,Antakly T,Cantin M,Chrétien M

更新日期：1983-09-01 00:00:00

abstract：:Cytochrome c has been purified to homogeneity from alligator liver (Alligator mississipiensis) using aluminum sulfate precipitation, CM-cellulose and gel-filtration chromatography, and reverse-phase HPLC. The protein exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an approxima...

journal_title：Archives of biochemistry and biophysics

authors： Barber MJ,Trimboli AJ,Clark M,Young C,Neame PJ

更新日期：1993-03-01 00:00:00

abstract：:Tripeptidyl-peptidase II (TPP II) is a cytosolic peptidase that has been implicated in fat formation and cancer, apparently independent of the enzymatic activity. In search for alternative functional regions, conserved motifs were identified and eleven signatures were constructed. Seven of the signatures covered previ...

journal_title：Archives of biochemistry and biophysics

authors： Eriksson S,Gutiérrez OA,Bjerling P,Tomkinson B

更新日期：2009-04-01 00:00:00

abstract：:Forskolin, a diterpene that exerts several pharmacological effects, activates adenylate cyclase in brain and in some other mammalian tissues. Properties of forskolin activation of adenylate cyclase from central nervous system of the dipterous Ceratitis capitata are described. The interaction of forskolin with the inse...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Municio AM

更新日期：1987-04-01 00:00:00

abstract：:Osteocytes sense extracellular mechanical stimuli and transduce them into biochemical signals to regulate bone remodeling. The function is also evidenced in orthodontic tooth movement. But the underlying mechanisms haven't been clarified. Autophagy is an evolutionarily conserved cellular catabolic process which affect...

journal_title：Archives of biochemistry and biophysics

authors： Li W,Zhao J,Sun W,Wang H,Pan Y,Wang L,Zhang WB

更新日期：2020-11-15 00:00:00

abstract：:Phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, Helix pomatia (gastropoda), and mussels, Mytilus edulis (bivalvia). Under denaturing conditions, both enzymes had a molecular mass of 82 kDa. In the presence of ATP-Mg2+, the enzymes were rapidly phosphorylated in vitro by th...

journal_title：Archives of biochemistry and biophysics

authors： Biethinger M,Hoffmann R,Hofer HW

更新日期：1991-06-01 00:00:00

abstract：:To establish a cell line expressing enhanced levels of beta-amyloid precursor protein (beta-APP), we constructed plasmid DNAs expressing beta-APP-751 mRNA and transfected them into COS-1 cells. Using a modified version of the reverse transcriptase polymerase chain reaction which is RNA sensitive to study the beta-APP ...

journal_title：Archives of biochemistry and biophysics

authors： Ramakrishna N,Smedman M,Gillam B

更新日期：1996-02-15 00:00:00

abstract：:Oxygenation of a tryptophan residue analog by ascorbate in the presence of catalytic amounts of iron(II) and ethylenediaminetetraacetic acid (EDTA) has been studied. Under physiological conditions, reaction of the tryptophan derivative (N-t-butoxycarbonyl-L-tryptophan) with Fe(II)-EDTA and ascorbate resulted mainly in...

journal_title：Archives of biochemistry and biophysics

authors： Uchida K,Enomoto N,Itakura K,Kawakishi S

更新日期：1990-05-15 00:00:00

abstract：:Appropriate dietary interventions may reduce the potentially damaging effects of free radicals generated during metabolism and various physiological conditions. We have investigated the effects of dietary vitamins C, E, beta-carotene, or selenium (Se) on the activity of endogenous antioxidant enzymes and respiratory c...

journal_title：Archives of biochemistry and biophysics

authors： Desai VG,Casciano D,Feuers RJ,Aidoo A

更新日期：2001-10-15 00:00:00

abstract：:The mechanisms underlying the inhibition of bile acid-induced apoptosis by cyclic AMP (cAMP) were studied in 24-h-cultured rat hepatocytes. Taurolithocholate 3-sulfate (TLCS, 100 micromol/l) led to a sustained activation of mitogen activated protein (MAP) kinases (JNK, p38(MAPK), and ERKs), dephosphorylation of protei...

journal_title：Archives of biochemistry and biophysics

authors： Graf D,Reinehr R,Kurz AK,Fischer R,Häussinger D

更新日期：2003-07-01 00:00:00

abstract：:A search of the chicken expressed sequence tag (EST) database identified 2 cDNA clones that appeared to represent members of the SULT1B and SULT1C enzyme families. These cDNAs were fully sequenced and found to contain full-length inserts. Phylogenetic analysis of the derived amino acid sequences clearly placed them as...

journal_title：Archives of biochemistry and biophysics

authors： Wilson LA,Reyns GE,Darras VM,Coughtrie MW

更新日期：2004-08-01 00:00:00

abstract：:NAD kinase from human neutrophils has been partially purified by sequential application of Red Agarose, ion-exchange, and gel-filtration chromatography. The enzyme has a broad pH optimum, 7.0-9.5, is strictly dependent upon the presence of Mg2+, and in the absence of calcium exhibits Km values of 0.6 and 0.9 mM for NA...

journal_title：Archives of biochemistry and biophysics

authors： Williams MB,Jones HP

更新日期：1985-02-15 00:00:00

abstract：:The earthworm-derived chemoattractant ES20 interacts with its G-protein-coupled receptors on the plasma membrane of vomeronasal (VN) sensory neurons of garter snakes, resulting in an increase in inositol trisphosphate [J. Biol. Chem. 269 (1994) 16867] and a rapid phosphorylation of the membrane-bound proteins, p42/44 ...

journal_title：Archives of biochemistry and biophysics

authors： Wang D,Liu W,Liu J,Chen P,Quan W,Halpern M

更新日期：2002-12-15 00:00:00

abstract：:The intracellular concentration of many steroids and xenobiotics is influenced by the membrane protein P-glycoprotein (Pgp). It has been inferred that the intracellular retention of many drugs that upregulate Pgp or modulate Pgp function might also be affected by Pgp. However, the ability of Pgp to influence the trans...

journal_title：Archives of biochemistry and biophysics

authors： Schuetz EG,Yasuda K,Arimori K,Schuetz JD

更新日期：1998-02-15 00:00:00

abstract：:The electron transfer reactions from FeII(CN)6(4-) and W(IV)(CN)8(4-) to the cupric ions in human copper, zinc superoxide dismutase were followed by the micro-stopped-flow method. The kinetic rate data clearly indicate that FeII(CN)6(4-) or W(IV)(CN)8(4-) first forms an adduct with the enzyme through the interaction w...

journal_title：Archives of biochemistry and biophysics

authors： Hirose J,Minakami M,Settu K,Tsukahara K,Ueda J,Ozawa T

更新日期：2000-11-15 00:00:00

abstract：:Interactions between loops 2, 2' and 4, known as the loop bundle, stabilize the active site of caspase-3. Loop 4 (L4) is of particular interest due to its location between the active site and the dimer interface. We have disrupted a salt bridge between K242 and E246 at the base of L4 to determine its role in overall c...

journal_title：Archives of biochemistry and biophysics

authors： Walters J,Swartz P,Mattos C,Clark AC

更新日期：2011-04-01 00:00:00

abstract：:In the presence of rat liver cytosol and homocysteine, trimethylselenonium ion (TMSe+) underwent time-dependent demethylation to dimethylselenide with the concurrent formation of methionine. Convenient methods were developed for assay of this activity using either radioactive methods based on the gamma emitting isotop...

journal_title：Archives of biochemistry and biophysics

authors： Goeger DE,Ganther HE

更新日期：1993-04-01 00:00:00

abstract：:The presence of a trifunctional beta-oxidation complex in pig heart and its relationship to the known long-chain enoyl-CoA hydratase (EC 4.2.1.74) from pig heart mitochondria were investigated. For this study, the complex was partially purified by chromatography on DEAE-cellulose in the absence of detergents and was p...

journal_title：Archives of biochemistry and biophysics

authors： Luo MJ,He XY,Sprecher H,Schulz H

更新日期：1993-07-01 00:00:00

abstract：:Fibrocartilage is found in tendon at sites where the tissue is subjected to transverse compressive loading in vivo. A significant characteristic of the tissue transition from tendon to fibrocartilage in bovine deep flexor tendon is increased gene expression, synthesis, and accumulation of both a large proteoglycan, ag...

journal_title：Archives of biochemistry and biophysics

authors： Robbins JR,Evanko SP,Vogel KG

更新日期：1997-06-15 00:00:00

abstract：:Heme and iron are essential to almost all forms of life. The strict maintenance of heme and iron homeostasis is essential to prevent cellular toxicity and the existence of systemic and intracellular regulation is fundamental. Cytosolic heme can be catabolized and detoxified by heme oxygenases (HOs). Interestingly, fre...

journal_title：Archives of biochemistry and biophysics

authors： Yanatori I,Richardson DR,Toyokuni S,Kishi F

更新日期：2019-09-15 00:00:00

abstract：:A marine snail digestive phospholipase A2 (mSDPL) was purified from delipidated hepatopancreas. Unlike known digestive phospholipases A2, which are 14 kDa proteins, the purified mSDPL has a molecular mass of about 30 kDa. It has a specific activity of about 180 U/mg measured at 50 degrees C and pH 8.5 using phosphatid...

journal_title：Archives of biochemistry and biophysics

authors： Zarai Z,Bacha AB,Horchani H,Bezzine S,Zouari N,Gargouri Y,Mejdoub H

更新日期：2010-02-15 00:00:00

abstract：:We previously found that vitamin K3 (menadione, 2-methyl-1,4-naphthoquinone) inhibits the activity of human mitochondrial DNA polymerase (pol) γ. In this study, we focused on plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone), and chemically synthesized novel plumbagins conjugated with C2:0 to C22:6 fatty acids (5-O-ac...

journal_title：Archives of biochemistry and biophysics

authors： Onodera T,Kuriyama I,Sakamoto Y,Kawamura M,Kuramochi K,Tsubaki K,Tabata A,Naganune H,Mizushina Y

更新日期：2015-05-01 00:00:00

abstract：:Earlier studies have shown that the Fur (ferric uptake regulation) protein acts as an anaerobic/aerobic repressor of MnSOD (sodA) expression. We found that the aerobic expression of sodA::lacZ fusion in a Fur- background to be threefold higher than in a Fur+ background. This effect of fur mutation was not seen in a st...

journal_title：Archives of biochemistry and biophysics

authors： Schrum LW,Hassan HM

更新日期：1994-03-01 00:00:00