当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > The effects of fur on the transcriptional and post-transcriptional regulation of MnSOD gene (sodA) in Escherichia coli.

The effects of fur on the transcriptional and post-transcriptional regulation of MnSOD gene (sodA) in Escherichia coli.

Abstract：

:Earlier studies have shown that the Fur (ferric uptake regulation) protein acts as an anaerobic/aerobic repressor of MnSOD (sodA) expression. We found that the aerobic expression of sodA::lacZ fusion in a Fur- background to be threefold higher than in a Fur+ background. This effect of fur mutation was not seen in a strain harboring the sodA+ gene instead of the sodA::lacZ fusion. However, we observed a proportionate increase in the concentrations of sodA::lacZ and sodA+ mRNAs in response to a mutation in the fur gene. These data suggest that the formation of active MnSOD is dependent on a functional fur gene. Indeed, we found that in a fur mutant iron was incorporated into SodA in place of manganese, thus creating inactive and/or partially active forms of the enzyme (i.e., Fe2SodA and/or Mn,FeSodA, respectively), resulting in little or no increase in total MnSOD activity. Thus, Fur plays the role of a repressor at the transcriptional level, but it also plays an indirect role at the post-transcriptional level where it affects the maturation of SodA into a fully active enzyme, Mn2SodA (MnSOD).

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Schrum LW,Hassan HM

doi

10.1006/abbi.1994.1115

subject

Has Abstract

pub_date

1994-03-01 00:00:00

pages

288-92

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(84)71115-5

journal_volume

309

pub_type

杂志文章

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