Role of the snake venom toxin jararhagin in proinflammatory pathogenesis: in vitro and in vivo gene expression analysis of the effects of the toxin.

abstract：:G. metallireducens bacterium has highly versatile respiratory pathways that provide the microorganism an enormous potential for many biotechnological applications. However, little is known about the structural and functional properties of its electron transfer components. In this work, the periplasmic cytochrome PpcA ...

journal_title：Archives of biochemistry and biophysics

authors： Portela PC,Fernandes TM,Dantas JM,Ferreira MR,Salgueiro CA

更新日期：2018-04-15 00:00:00

abstract：:The glucose transporter of the bacterial phosphotransferase system mediates sugar transport across the cytoplasmic membrane concomitant with sugar phosphorylation. It consists of a cytoplasmic subunit IIA(Glc) and the transmembrane subunit IICB(Glc). IICB(Glc) was purified to homogeneity by urea/alkali washing of memb...

journal_title：Archives of biochemistry and biophysics

authors： Zhuang J,Gutknecht R,Flükiger K,Hasler L,Erni B,Engel A

更新日期：1999-12-01 00:00:00

abstract：:Uridine kinase is the rate-limiting enzyme in the pyrimidine salvage pathway of all mammalian cells. A cDNA for uridine kinase from mouse brain has been isolated, sequenced, and characterized. This is the first report of a complete nucleotide sequence for mammalian uridine kinase. The isolated cDNA is only 95% complet...

journal_title：Archives of biochemistry and biophysics

authors： Ropp PA,Traut TW

更新日期：1996-12-01 00:00:00

abstract：:The gene p8 encodes for a small cytoprotective protein with no apparent enzymatic activity being proposed to act as co-transcription factor whose expression is increased during inflammation. Recent data from astrocytes demonstrates that p8 suppression leads to induction of heme oxygenase 1 (HO-1). Here, we assessed th...

journal_title：Archives of biochemistry and biophysics

authors： Weis S,Bielow T,Sommerer I,Iovanna J,Malicet C,Mössner J,Hoffmeister A

更新日期：2015-01-01 00:00:00

abstract：:In this study, chemical chaperone like function of cyanidin-3-O-glucoside (C3G) was investigated through fluorescence spectroscopy, UV-visible spectroscopy, circular dichroism spectroscopy, confocal microscopy, scanning electron microscopy and molecular docking studies. Early and advanced glycation inhibitory effect w...

journal_title：Archives of biochemistry and biophysics

authors： Prasanna G,Jing P

更新日期：2018-04-02 00:00:00

abstract：:A glucose analog, N-(bromoacetyl)-D-glucosamine (GlcNBrAc), previously used to label the glucose binding sites of rat muscle Type II and bovine brain Type I hexokinases, also inactivates rat brain hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) with pseudo-first-order kinetics. Inactivation occurs predomina...

journal_title：Archives of biochemistry and biophysics

authors： Schirch DM,Wilson JE

更新日期：1987-05-01 00:00:00

abstract：:Two histone acetyltransferases, A and B, have been extracted and partially purified from yeast cells. The purification scheme included ammonium sulfate precipitation, and chromatography on DEAE-Sepharose and Sephadex G-200. The basic properties of both enzymes closely correspond to those of acetyltransferase A and B f...

journal_title：Archives of biochemistry and biophysics

authors： Lopez-Rodas G,Perez-Ortin JE,Tordera V,Salvador ML,Franco L

更新日期：1985-05-15 00:00:00

abstract：:Erythropoietin was purified to homogeneity from the culture medium of a baby hamster kidney cell line stably transfected with a human erythropoietin gene. A three-step procedure was used, which included affinity chromatography, ion-exchange chromatography, and reverse-phase chromatography. Purity of the protein was co...

journal_title：Archives of biochemistry and biophysics

authors： Broudy VC,Tait JF,Powell JS

更新日期：1988-09-01 00:00:00

abstract：:The mammalian aldehyde dehydrogenase-3 gene (ALDH3) exhibits several aspects of tissue-specific expression. Certain normal tissues, such as the cornea, constitutively express ALDH3 at very high levels. Other tissues, such as normal liver, do not express ALDH3. In liver, ALDH3 is inducible by polycyclic aromatic hydroc...

journal_title：Archives of biochemistry and biophysics

authors： Boesch JS,Miskimins R,Miskimins WK,Lindahl R

更新日期：1999-01-15 00:00:00

abstract：:Phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, Helix pomatia (gastropoda), and mussels, Mytilus edulis (bivalvia). Under denaturing conditions, both enzymes had a molecular mass of 82 kDa. In the presence of ATP-Mg2+, the enzymes were rapidly phosphorylated in vitro by th...

journal_title：Archives of biochemistry and biophysics

authors： Biethinger M,Hoffmann R,Hofer HW

更新日期：1991-06-01 00:00:00

abstract：:Stem cells maintain their self-renewal and multipotency capacities through a self-organizing network of transcription factors and intracellular pathways activated by extracellular signaling from the microenvironment or "niche" in which they reside in vivo. In the adult mammalian brain new neurons continue to be genera...

journal_title：Archives of biochemistry and biophysics

authors： Porlan E,Perez-Villalba A,Delgado AC,Ferrón SR

更新日期：2013-06-01 00:00:00

abstract：:Electron spin resonance (ESR) spectroscopy has been employed to examine the role of tetraperoxochromate (V) ions (CrO3-8) and other Cr(V) species in the generation of hydroxyl (OH) radicals in the reaction of Cr(VI) with H2O2 in biological media. In contrast to earlier suggestions, the present ESR studies using crysta...

journal_title：Archives of biochemistry and biophysics

authors： Shi XL,Dalal NS

更新日期：1990-08-15 00:00:00

abstract：:MICALs form a conserved multidomain protein family essential for cytoskeletal rearrangements. To complement structural information available, we produced the FAD-containing monooxygenase-like domain of human MICAL-1 (MICAL-MO) in forms differing for the presence and location of a His-tag, which only influences the pro...

journal_title：Archives of biochemistry and biophysics

authors： Zucchini D,Caprini G,Pasterkamp RJ,Tedeschi G,Vanoni MA

更新日期：2011-11-01 00:00:00

abstract：:Reductant-independent ATPase activity was initiated and studied for Azotobacter vinelandii and Clostridium pasteuranium nitrogenase proteins (Av1, Cp1 and Av2, Cp2, 1 designating the iron molybdenum protein and 2 the iron protein) and their heterologous crosses by two methods: (1) allowing dithionite to be depleted fr...

journal_title：Archives of biochemistry and biophysics

authors： Larsen C,Christensen S,Watt GD

更新日期：1995-11-10 00:00:00

abstract：:Quantitative estimation of cytosolic Ca2+- and phospholipid-dependent protein kinase (PKC) activity was performed by polyacrylamide gel electrophoresis under nondenaturating conditions (PAGE). With this method less than 50 micrograms of cytosol protein can be accurately quantitated for PKC activity. The amount of cyto...

journal_title：Archives of biochemistry and biophysics

authors： Fabbro D,Jungmann RA,Eppenberger U

更新日期：1985-05-15 00:00:00

abstract：:The oxidation of the heme iron of metmyoglobin by H2O2 yields an oxo ferryl complex (FeIV = O), similar to Compound II of peroxidases, as well as a protein radical; this high oxidation state of myoglobin is known as ferrylmyoglobin. The interaction of Trolox, a water-soluble vitamin E analog, with ferrylmyoglobin enta...

journal_title：Archives of biochemistry and biophysics

authors： Giulivi C,Romero FJ,Cadenas E

更新日期：1992-12-01 00:00:00

abstract：:We have previously shown that rat hepatic cytochromes P450f, P450g, P450h, and P450i possess a high degree of immunochemical and, presumably, structural relatedness. Polyclonal antibodies directed against cytochromes P450f and P450g were made monospecific by immunoabsorption against the cross-reactive proteins. The sp...

journal_title：Archives of biochemistry and biophysics

authors： Bandiera S,Ryan DE,Levin W,Thomas PE

更新日期：1986-08-01 00:00:00

abstract：:The reversible oxidation of protein cysteine residues is well recognized as an important regulatory mechanism in redox-dependent cell signaling. Cysteine oxidation is diverse in nature and involves various post-translational modifications (sulfenic acids, disulfides, etc.) and the specific functional or structural imp...

journal_title：Archives of biochemistry and biophysics

authors： Heppner DE,Janssen-Heininger YMW,van der Vliet A

更新日期：2017-02-15 00:00:00

abstract：:Dopamine beta-hydroxylase present in the soluble matrix of bovine adrenal medullary chromaffin granules contains biantennary complex oligosaccharides and high-mannose oligosaccharides in a molar ratio of approximately 2:1. The high-mannose oligosaccharides contain an average of six mannose residues. The largest biante...

journal_title：Archives of biochemistry and biophysics

authors： Margolis RK,Finne J,Krusius T,Margolis RU

更新日期：1984-02-01 00:00:00

abstract：:Three iodinated derivatives of ubiquitin have been synthesized and these derivatives have been characterized in the ubiquitin-dependent protein degradation system. With chloramine-T as the oxidant, a derivative containing monoiodotyrosine is formed in the presence of 1 M KI and a derivative containing diiodotyrosine i...

journal_title：Archives of biochemistry and biophysics

authors： Cox MJ,Haas AL,Wilkinson KD

更新日期：1986-11-01 00:00:00

abstract：:The portrait of osteoblasts and osteocytes has been subjected to a revision, since a large body of evidence is attributing these cells amazing roles both inside and outside the bone. The osteoblast, long confined to its bone building function, is actually a very eclectic cell, actively regulating osteoclast formation ...

journal_title：Archives of biochemistry and biophysics

authors： Capulli M,Paone R,Rucci N

更新日期：2014-11-01 00:00:00

abstract：:Cystalysin, the key virulence factor in the bacterium Treponema denticola responsible for periodontis, is a pyridoxal 5'-phosphate (PLP) enzyme which catalyzes, in addition to alpha,beta-elimination of L-cysteine, racemization and transamination of both enantiomers of alanine. In this paper several indicators have bee...

journal_title：Archives of biochemistry and biophysics

authors： Cellini B,Montioli R,Bossi A,Bertoldi M,Laurents DV,Voltattorni CB

更新日期：2006-11-01 00:00:00

abstract：:Under physiological conditions the uptake of transferrin-bound iron by reticulocytes involves transferrin binding to membrane receptors followed by endocytosis, release of iron from the transferrin within endosomes, and recycling of apotransferrin to the cell surface. However, as shown in the present work, iron uptake...

journal_title：Archives of biochemistry and biophysics

authors： Hodgson LL,Quail EA,Morgan EH

更新日期：1994-01-01 00:00:00

abstract：:Solubilized NADPH-cytochrome c (P450) reductase was purified to homogeneity from an extract of spearmint (Mentha spicata) glandular trichomes by dye-ligand interaction chromatography on Matrex-Gel Red A and affinity chromatography on 2', 5'-adenosine diphosphate agarose. SDS-PAGE of the purified enzyme preparation rev...

journal_title：Archives of biochemistry and biophysics

authors： Ponnamperuma K,Croteau R

更新日期：1996-05-01 00:00:00

abstract：:Human programmed cell death 5 (PDCD5) is a translocatory protein playing an important role in the apoptotic process of cells. Although there are accumulated data about PDCD5 function, the correlation of the structure with the function of PDCD5 has not been investigated. Here, we report the studies of structure-functio...

journal_title：Archives of biochemistry and biophysics

authors： Yao H,Xu L,Feng Y,Liu D,Chen Y,Wang J

更新日期：2009-06-15 00:00:00

abstract：:Vascular smooth muscle cell (VSMC) proliferation is a key event in the progression of atherosclerosis. Induction of both c-fos (through the transcription factor Elk-1) and c-jun, both immediate early genes, is important for the stimulation of VSMC proliferation and migration. It was earlier found that p38 mitogen-acti...

journal_title：Archives of biochemistry and biophysics

authors： Zhao M,Liu Y,Bao M,Kato Y,Han J,Eaton JW

更新日期：2002-04-15 00:00:00

abstract：:Using specific testosterone 16 alpha-hydroxylase activity as the basis for selection of fractions during purification, the cytochrome P-450 ("I"-P-450(16)alpha) has been isolated from livers of phenobarbital-treated 129/J female mice [K. Devore, N. Harada, and M. Negishi (1985) Biochemistry, 24, 5632-5637]. An antibod...

journal_title：Archives of biochemistry and biophysics

authors： Noshiro M,Serabjit-Singh CJ,Bend JR,Negishi M

更新日期：1986-02-01 00:00:00

abstract：:Glycophorin from human red blood cells was exposed to ozone in aqueous solution. Amino acid analysis of glycophorin exposed to a 10-fold molar excess of ozone showed that the only residue affected was methionine. Both methionine residues of the protein were oxidized to methionine sulfoxide. Exposure of the oxidized pr...

journal_title：Archives of biochemistry and biophysics

authors： Banerjee SK,Mudd JB

更新日期：1992-05-15 00:00:00

abstract：:Autophagy, a ubiquitous degradation pathway, is important for the survival and homeostasis of cells. Previous studies have demonstrated the role of autophagy in host defense against bacterial infection, but the importance of autophagy in the intestinal epithelium for the regulation of bacterial infection has not been ...

journal_title：Archives of biochemistry and biophysics

authors： Inoue J,Nishiumi S,Fujishima Y,Masuda A,Shiomi H,Yamamoto K,Nishida M,Azuma T,Yoshida M

更新日期：2012-05-01 00:00:00

abstract：:The mechanisms underlying the inhibition of bile acid-induced apoptosis by cyclic AMP (cAMP) were studied in 24-h-cultured rat hepatocytes. Taurolithocholate 3-sulfate (TLCS, 100 micromol/l) led to a sustained activation of mitogen activated protein (MAP) kinases (JNK, p38(MAPK), and ERKs), dephosphorylation of protei...

journal_title：Archives of biochemistry and biophysics

authors： Graf D,Reinehr R,Kurz AK,Fischer R,Häussinger D

更新日期：2003-07-01 00:00:00